From Sequence to Structure
... acceptor. Histidine is perhaps the most versatile of all the amino acids in this regard, which explains why it is also the residue most often found in enzyme active sites. It has two titratable –N–H groups, each with pKa values around 6. When one of these –N–H groups loses a proton, however, the pKa ...
... acceptor. Histidine is perhaps the most versatile of all the amino acids in this regard, which explains why it is also the residue most often found in enzyme active sites. It has two titratable –N–H groups, each with pKa values around 6. When one of these –N–H groups loses a proton, however, the pKa ...
Chemistry 433 BIOCHEMISTRY LABORATORY MANUAL
... The preferred page layout for lab report submission has the body of your paper in double-spaced text.) Many scientists have their own preferred ways of writing papers. Most scientists, however, use an iterative process of writing, in which they write the paper, and then rewrite it several times befo ...
... The preferred page layout for lab report submission has the body of your paper in double-spaced text.) Many scientists have their own preferred ways of writing papers. Most scientists, however, use an iterative process of writing, in which they write the paper, and then rewrite it several times befo ...
Aqueous Solutions
... Figure 4.2: Polar water molecules interact with the positive and negative ions of a salt assisting in the dissolving process. ...
... Figure 4.2: Polar water molecules interact with the positive and negative ions of a salt assisting in the dissolving process. ...
EFFECT OF LEWIS ACID IN TiCl4/MgCl2/THF/AlCl3 CATALYST
... For polymerization reaction, TEA was used as cocatalyst and hexane was used as a medium solvent. The Al/Ti molar ratio was 140. The activity result for addition of CaCl2, ZnCl2and FeCl2was 979, 1009 and 1476 kgPE/molTi.h, respectively. The addition of FeCl2 in TiCl4/MgCl2/THF/AlCl3 catalyst system e ...
... For polymerization reaction, TEA was used as cocatalyst and hexane was used as a medium solvent. The Al/Ti molar ratio was 140. The activity result for addition of CaCl2, ZnCl2and FeCl2was 979, 1009 and 1476 kgPE/molTi.h, respectively. The addition of FeCl2 in TiCl4/MgCl2/THF/AlCl3 catalyst system e ...
Enzymes for Pharma Applications
... Fungal Lactase: Lactose intolerance is a very common disorder in infants and older persons. Lactazyme (lactase) helps in digestion of lactose. Lactazyme is a purified enzyme preparation isolated from selected strain of Aspergillus Oryzae by fermentation process. Lactazyme hydrolyzes lactose t produc ...
... Fungal Lactase: Lactose intolerance is a very common disorder in infants and older persons. Lactazyme (lactase) helps in digestion of lactose. Lactazyme is a purified enzyme preparation isolated from selected strain of Aspergillus Oryzae by fermentation process. Lactazyme hydrolyzes lactose t produc ...
Rabbit Reticulocyte Lysate Technical Manual
... templates, and 2) coupled transcription/translation systems for DNA templates. Many factors influence the choice of an in vitro translation system; some of these considerations are summarized in Section 11.B, Related Products. The reaction conditions provided below are optimized for the Luciferase C ...
... templates, and 2) coupled transcription/translation systems for DNA templates. Many factors influence the choice of an in vitro translation system; some of these considerations are summarized in Section 11.B, Related Products. The reaction conditions provided below are optimized for the Luciferase C ...
The Early Interaction of the Outer Membrane Protein PhoE with
... MC4100 (F⫺ ⌬lacU169 araD136 rpsL thi relA) was used for the spheroplast labeling experiments and for the isolation of lysate and inner membrane vesicles (19). MC4100 and RC354c, containing a deletion in the skp gene (17), were used for the isolation of periplasmic extracts (20). BG87 (21) was used f ...
... MC4100 (F⫺ ⌬lacU169 araD136 rpsL thi relA) was used for the spheroplast labeling experiments and for the isolation of lysate and inner membrane vesicles (19). MC4100 and RC354c, containing a deletion in the skp gene (17), were used for the isolation of periplasmic extracts (20). BG87 (21) was used f ...
Molecular Machines
... Jeffrey Brinker and coworkers at The molecular elevator stems Sandia National Laboratories in from one of the first molecular Albuquerque, US, have used this constructions to be presented as a principle to make light-switchable mechanical device: the molecular valves for opening and closing the shut ...
... Jeffrey Brinker and coworkers at The molecular elevator stems Sandia National Laboratories in from one of the first molecular Albuquerque, US, have used this constructions to be presented as a principle to make light-switchable mechanical device: the molecular valves for opening and closing the shut ...
ProteoChem`s Current Catalogue.
... Protein Crosslinkers Protein crosslinkers are chemical compounds used to covalently bind, or conjugate, biomolecules together. In proteomics, "protein crosslinking" refers to utilizing protein crosslinkers to conjugate peptides or proteins together or to immobilize proteins to a solid support. ...
... Protein Crosslinkers Protein crosslinkers are chemical compounds used to covalently bind, or conjugate, biomolecules together. In proteomics, "protein crosslinking" refers to utilizing protein crosslinkers to conjugate peptides or proteins together or to immobilize proteins to a solid support. ...
Refining the Definition of Plant Mitochondrial
... Tom20 (Perry et al., 2006; Lister et al., 2007). However, the NMR structure of plant Tom20 reveals a similar hydrophobic binding pocket. This has been highlighted as a case of convergent evolution of a receptor that uses a similar mechanism of binding to recognize presequences (Lister and Whelan, 20 ...
... Tom20 (Perry et al., 2006; Lister et al., 2007). However, the NMR structure of plant Tom20 reveals a similar hydrophobic binding pocket. This has been highlighted as a case of convergent evolution of a receptor that uses a similar mechanism of binding to recognize presequences (Lister and Whelan, 20 ...
2_5 Slides
... Possible research questions to investigate (independent variable)? • What is the effect of substrate concentration? • What is the effect of temperature? • What is the effect of pH? • Which type of yeast has a higher concentration of catalase? Important things to consider: • How are you going to vary ...
... Possible research questions to investigate (independent variable)? • What is the effect of substrate concentration? • What is the effect of temperature? • What is the effect of pH? • Which type of yeast has a higher concentration of catalase? Important things to consider: • How are you going to vary ...
The hepatitis C virus Core protein is a potent nucleic acid chaperone
... previous reports (22), this enhancement is also not a general property of DNA binding proteins nor of positively charged peptides since the single-stranded DNA binding protein T4gp32 or the DNCp7 protein (pI = 9.9) were unable to promote duplex formation at similar concentrations (Fig. 2B, lanes 16± ...
... previous reports (22), this enhancement is also not a general property of DNA binding proteins nor of positively charged peptides since the single-stranded DNA binding protein T4gp32 or the DNCp7 protein (pI = 9.9) were unable to promote duplex formation at similar concentrations (Fig. 2B, lanes 16± ...
Preparation of Lentiviral expression construct DNA
... than a similar competitor kit (Fig.1). HTS compatible - Optimized for use on 96-well plate readers for high-throughput screening assays. Single strip (8-well) assay can also be performed. Fast - 3.5 hours from preparation to detection. Quantitative – The active and purified human recombinant p53 pro ...
... than a similar competitor kit (Fig.1). HTS compatible - Optimized for use on 96-well plate readers for high-throughput screening assays. Single strip (8-well) assay can also be performed. Fast - 3.5 hours from preparation to detection. Quantitative – The active and purified human recombinant p53 pro ...
University of Groningen Cross-linking of dimeric CitS and GltS
... pBADCitS situated in between the enterokinase site and CitS encoding sequences yielding plasmid pCitSBAD1. Plasmid pCitS-BAD260 was obtained by replacing a Bpu1102I-XbaI fragment of pBADCitS with the corresponding fragment including the BAD encoding sequence of pSB260. Plasmids pBADGlts and pN356C e ...
... pBADCitS situated in between the enterokinase site and CitS encoding sequences yielding plasmid pCitSBAD1. Plasmid pCitS-BAD260 was obtained by replacing a Bpu1102I-XbaI fragment of pBADCitS with the corresponding fragment including the BAD encoding sequence of pSB260. Plasmids pBADGlts and pN356C e ...
How Translocons Select Transmembrane Helices
... Membrane protein assembly. (a) The machinery of membrane protein assembly. (Step 1) A ribosome translating the mRNA of a protein targeted for secretion across or insertion into membranes and a signal of a recognition particle (SRP), which is a GTPase. The structures of ribosomes are reviewed in Refe ...
... Membrane protein assembly. (a) The machinery of membrane protein assembly. (Step 1) A ribosome translating the mRNA of a protein targeted for secretion across or insertion into membranes and a signal of a recognition particle (SRP), which is a GTPase. The structures of ribosomes are reviewed in Refe ...
Digestive Enzymes Plus
... matter; they are essential to maintain all the body functions working properly. Each enzyme has a specific function in the body that no other enzyme can carry out. Digestive enzymes are specifically involved in the process of digestion. They are considered proteolytic enzymes since they only work on ...
... matter; they are essential to maintain all the body functions working properly. Each enzyme has a specific function in the body that no other enzyme can carry out. Digestive enzymes are specifically involved in the process of digestion. They are considered proteolytic enzymes since they only work on ...
BIOCHEMISTRY
... Pathology laboratory is to provide biochemical information for the management of patients. Biochemical tests are used extensively in medicine, both in relation to diseases that have an obvious metabolic basis (diabetes mellitus, hypo- or hyperthyroidism, etc.) and those in which biochemical changes ...
... Pathology laboratory is to provide biochemical information for the management of patients. Biochemical tests are used extensively in medicine, both in relation to diseases that have an obvious metabolic basis (diabetes mellitus, hypo- or hyperthyroidism, etc.) and those in which biochemical changes ...
Significance of Intestinal Digestion of Dietary Protein
... amount of intact feed or feed that has been preincubated in the rumen is placed in bags. The bags are then preincubated in a pepsin-HCl solution or directly introduced into the duodenum and subsequently collected from the ileum, or more typically from the feces. Bags are then washed to remove endoge ...
... amount of intact feed or feed that has been preincubated in the rumen is placed in bags. The bags are then preincubated in a pepsin-HCl solution or directly introduced into the duodenum and subsequently collected from the ileum, or more typically from the feces. Bags are then washed to remove endoge ...
Click
... Question 21 is an extra credit questions on pharmaceutical chemistry. The points earned from this question will be added to your total score for part A of this exam, but cannot raise the score above 100. 21. (6) After each term in write one choice from the choices list that is the best match. The ch ...
... Question 21 is an extra credit questions on pharmaceutical chemistry. The points earned from this question will be added to your total score for part A of this exam, but cannot raise the score above 100. 21. (6) After each term in write one choice from the choices list that is the best match. The ch ...
The p101 subunit of PI3Kγ restores activation by Gβ mutants
... and 1 mM β-glycerophosphate (vesicle buffer) as described previously [24,45] with some modifications. A 30 μl lipid vesicle mixture, containing 320 μM phosphatidylethanolamine, 300 μM phosphatidylserine, 140 μM phosphatidylcholine and 30 μM sphingomyelin supplemented with 40 μM PtdIns(4,5)P2 was dri ...
... and 1 mM β-glycerophosphate (vesicle buffer) as described previously [24,45] with some modifications. A 30 μl lipid vesicle mixture, containing 320 μM phosphatidylethanolamine, 300 μM phosphatidylserine, 140 μM phosphatidylcholine and 30 μM sphingomyelin supplemented with 40 μM PtdIns(4,5)P2 was dri ...
The Amino Acid Sequence Contains Leucine-Rich
... 1,200g for 15 minutes to sediment platelets, which were subsequently washed three times in 10 mmol/L HEPES, pH 7.6 (1 mmol/L in EDTA, 0.15 mol/L in NaCI). GPV was extracted from platelet plasma membrane by incubating platelets (2 x lo9platelets/ Bernard-Soulier syndrome, a rare inherited bleeding di ...
... 1,200g for 15 minutes to sediment platelets, which were subsequently washed three times in 10 mmol/L HEPES, pH 7.6 (1 mmol/L in EDTA, 0.15 mol/L in NaCI). GPV was extracted from platelet plasma membrane by incubating platelets (2 x lo9platelets/ Bernard-Soulier syndrome, a rare inherited bleeding di ...
NMR-BIO kits for large proteins labeling
... Detection of long-range nOes in supramolecular assemblies: NMR-Bio scrambling free labeling solutions are optimized for the extraction of precise and long-range nOe distance restraints between methyl probes in perdeut erated proteins. Compared to standard 2-keto acids, NMR-Bio acetolact ate precurso ...
... Detection of long-range nOes in supramolecular assemblies: NMR-Bio scrambling free labeling solutions are optimized for the extraction of precise and long-range nOe distance restraints between methyl probes in perdeut erated proteins. Compared to standard 2-keto acids, NMR-Bio acetolact ate precurso ...
Role of Streptococcus sanguinis sortase A in bacterial
... bacterium initiates aggregation by other oral bacteria and maturation of dental plaque. Additionally, S. sanguinis is frequently isolated from infective endocarditis patients. We investigated the functions of sortase A (SrtA), which cleaves LPXTG-containing proteins and anchors them to the bacterial ...
... bacterium initiates aggregation by other oral bacteria and maturation of dental plaque. Additionally, S. sanguinis is frequently isolated from infective endocarditis patients. We investigated the functions of sortase A (SrtA), which cleaves LPXTG-containing proteins and anchors them to the bacterial ...
Acetate kinase from CIostridiurn acetobutylicurn : a highly specific
... loaded onto a C 10/20 column containing 8 ml Procion Blue HE3B (Pharmacia) equilibrated with imidazole buffer. The column was washed with 40 ml of the same buffer, and the enzyme was eluted by an ATP gradient (0-1 mM ATP in imidazole buffer; 80 ml) at a maximal flow rate of 10 ml h-'. The fractions ...
... loaded onto a C 10/20 column containing 8 ml Procion Blue HE3B (Pharmacia) equilibrated with imidazole buffer. The column was washed with 40 ml of the same buffer, and the enzyme was eluted by an ATP gradient (0-1 mM ATP in imidazole buffer; 80 ml) at a maximal flow rate of 10 ml h-'. The fractions ...