Protein sequence databases
... The concept of protein superfamilies, families and domains is one of the oldest in computational biology. Back in the 60s, when the first protein sequence database was published as printed version, Margaret Dayhoff defined the basic principles of this discipline with only a small number of sequences ...
... The concept of protein superfamilies, families and domains is one of the oldest in computational biology. Back in the 60s, when the first protein sequence database was published as printed version, Margaret Dayhoff defined the basic principles of this discipline with only a small number of sequences ...
A 1-Megadalton Translocation Complex Containing
... located in the inner and outer envelope membranes. At the inner membrane, many Tic components have been identified and characterized, but it remains unclear how these Tic proteins are organized to form a protein-conducting channel or whether a stable Tic core complex that binds translocating preprot ...
... located in the inner and outer envelope membranes. At the inner membrane, many Tic components have been identified and characterized, but it remains unclear how these Tic proteins are organized to form a protein-conducting channel or whether a stable Tic core complex that binds translocating preprot ...
Purification and Physico-Chemical Analysis of Fractions from the
... toxicity of Ribi's endotoxin and produced a parallel decrease in the average sedimentation coefficient of the solute, although the 'acid hapten' obtained by this process (s = 1.4 S) retained certain antigenic characteristics of the parent endotoxin (Ribi et al. 1962). By using non-hydrolytic conditi ...
... toxicity of Ribi's endotoxin and produced a parallel decrease in the average sedimentation coefficient of the solute, although the 'acid hapten' obtained by this process (s = 1.4 S) retained certain antigenic characteristics of the parent endotoxin (Ribi et al. 1962). By using non-hydrolytic conditi ...
sol-gel chemistry of transition metal oxides
... One unique property of the sol-gel process the molecular precursor ...
... One unique property of the sol-gel process the molecular precursor ...
Enzyme
... substrates bind. Enzyme catalysis involves molecular motion and the collision of substrates with the active site. Temperature, pH and substrate concentration affect Students should be able to sketch graphs to the rate of activity of enzymes. show the expected effects of temperature, pH and substrate ...
... substrates bind. Enzyme catalysis involves molecular motion and the collision of substrates with the active site. Temperature, pH and substrate concentration affect Students should be able to sketch graphs to the rate of activity of enzymes. show the expected effects of temperature, pH and substrate ...
Fluorescent Probe Studies of Proteins
... Control cuvette. (No phosphorylase kinase added). The slight downward trend illustrates that some photochemical decomposition occurs as a result of illumination. Enzyme-catalyzed assay containing 5.7 µg/ml phosphorylase kinase + 0.1 mM Ca2+. Sample was kept in the cuvette well throughout the reactio ...
... Control cuvette. (No phosphorylase kinase added). The slight downward trend illustrates that some photochemical decomposition occurs as a result of illumination. Enzyme-catalyzed assay containing 5.7 µg/ml phosphorylase kinase + 0.1 mM Ca2+. Sample was kept in the cuvette well throughout the reactio ...
Proteome analysis of tobacco BY-2 cell culture - ETH E
... mature plastids. During fruit ripening, chloroplasts differentiate into nonphotosynthetic chromoplasts and during this process chlorophyll is degraded and large amounts of carotenoids accumulate. The aim of this work is to advance the understanding of the proteome and biochemical functions of differ ...
... mature plastids. During fruit ripening, chloroplasts differentiate into nonphotosynthetic chromoplasts and during this process chlorophyll is degraded and large amounts of carotenoids accumulate. The aim of this work is to advance the understanding of the proteome and biochemical functions of differ ...
Protein synthesis meets ABC ATPases: new roles for Rli1/ABCE1
... dual reporter system, indicating a lower efficiency of termination. Conversely, upregulation of Rli1 partly suppresses the increased read-through caused by certain mutations of eRF1. Although the mechanism by which Rli1 affects translation termination is not understood, the results of the Krebber la ...
... dual reporter system, indicating a lower efficiency of termination. Conversely, upregulation of Rli1 partly suppresses the increased read-through caused by certain mutations of eRF1. Although the mechanism by which Rli1 affects translation termination is not understood, the results of the Krebber la ...
chemistry 103 - chem.uwec.edu
... Example: Calculate the pH of a buffer system containing 1.0 M CH3CO2H and 1.0 M NaCH3CO2. What is the pH of the buffer after the addition of 0.10 moles of gaseous HCl to 1.00 liter of the buffer solution? The Ka for acetic acid is 1.8 x 10-5. Because acetic acid is a weak acid, we can ignore the sm ...
... Example: Calculate the pH of a buffer system containing 1.0 M CH3CO2H and 1.0 M NaCH3CO2. What is the pH of the buffer after the addition of 0.10 moles of gaseous HCl to 1.00 liter of the buffer solution? The Ka for acetic acid is 1.8 x 10-5. Because acetic acid is a weak acid, we can ignore the sm ...
Adaptation of enzymes to temperature: searching for basic ``strategies``
... et al., 1955), Bullock concluded that, ‘‘Little can be said. . . about the mechanism or mechanisms by which some poikilothermic animals accomplish their relative independence of temperature.’’ The following decade witnessed considerable progress in the analysis of biochemical mechanisms of temperatu ...
... et al., 1955), Bullock concluded that, ‘‘Little can be said. . . about the mechanism or mechanisms by which some poikilothermic animals accomplish their relative independence of temperature.’’ The following decade witnessed considerable progress in the analysis of biochemical mechanisms of temperatu ...
published a paper
... possible that the PRPP product resulted from cross-contamination with these ribozymes. To eliminate this possibility, the pR1PR products and pR1-PRPP product were purified separately from an APM gel, reverse transcribed, and subjected to sequencing. Thirteen sequences derived from the pR1-PR product ...
... possible that the PRPP product resulted from cross-contamination with these ribozymes. To eliminate this possibility, the pR1PR products and pR1-PRPP product were purified separately from an APM gel, reverse transcribed, and subjected to sequencing. Thirteen sequences derived from the pR1-PR product ...
HiTrap Heparin HP,1 ml and 5 ml
... pH stability, long term refers to the pH interval where the medium is stable over a long period of time without adverse effects on its subsequent chromatographic performance. pH stability, short term refers to the pH interval for regeneration, cleaning-in-place and sanitization procedure ...
... pH stability, long term refers to the pH interval where the medium is stable over a long period of time without adverse effects on its subsequent chromatographic performance. pH stability, short term refers to the pH interval for regeneration, cleaning-in-place and sanitization procedure ...
Gel Electrophoresis and Amino Acid Analysis of the Nonprotein
... Gel Electrophoresis Sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) was performed by the Fling and Gregerson (1986) modification of the Laemmli (1970) procedure using a model SE 600 cell (Hoefer Scientific Instruments, San Francisco, CA). AcrylAide (FMC BioProducts, Rockland, ME ...
... Gel Electrophoresis Sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) was performed by the Fling and Gregerson (1986) modification of the Laemmli (1970) procedure using a model SE 600 cell (Hoefer Scientific Instruments, San Francisco, CA). AcrylAide (FMC BioProducts, Rockland, ME ...
Purification and Some: Characteristics of a Monomeric Racemase
... zyme solution was incubated in 0.1 M CHES-NaOH buffer (pH 9.0) and 30mM D-alanine for 30min in a final volume of 1 ml. The reaction was terminated by boiling the mixture for lOmin, and then the boiled mixture was incubated in 0.1 M CHES-NaOH buffer (pH 9.0), 2.5 mM NAD+, and 30 units of L-alanine de ...
... zyme solution was incubated in 0.1 M CHES-NaOH buffer (pH 9.0) and 30mM D-alanine for 30min in a final volume of 1 ml. The reaction was terminated by boiling the mixture for lOmin, and then the boiled mixture was incubated in 0.1 M CHES-NaOH buffer (pH 9.0), 2.5 mM NAD+, and 30 units of L-alanine de ...
CLINICAL CHEMISTRY CHAPTER 5
... • Labeled Immunoassays – Antigen or antibody is labeled ( tagged ) with a substance that can be detected later on and allows for the detection of an antibody – antigen ...
... • Labeled Immunoassays – Antigen or antibody is labeled ( tagged ) with a substance that can be detected later on and allows for the detection of an antibody – antigen ...
chem 100 class notes - Louisiana Tech University
... Classification of compounds as acids, bases and salts Most of the chemical compounds can be classified either as an acid, a base or a salt. Acids: Acids are compounds that increase the hydrogen ion concentration when dissolved in water. They usually have at least one removable hydrogen atom attached ...
... Classification of compounds as acids, bases and salts Most of the chemical compounds can be classified either as an acid, a base or a salt. Acids: Acids are compounds that increase the hydrogen ion concentration when dissolved in water. They usually have at least one removable hydrogen atom attached ...
Molecular design of the photosystem II light
... This hypothesis for the role of PsbS has been subject to intensive investigation. Evidence for proton binding has come from the identification of binding sites for DCCD (Dominici et al., 2002), and from the inhibition of qE by site-directed mutagenesis of potential proton binding residues (Li et al. ...
... This hypothesis for the role of PsbS has been subject to intensive investigation. Evidence for proton binding has come from the identification of binding sites for DCCD (Dominici et al., 2002), and from the inhibition of qE by site-directed mutagenesis of potential proton binding residues (Li et al. ...
Protein Applications in Sports Nutrition—Part I
... computed, the upper limit of the recommended protein intake was determined to be 1.33 g$kg21$d21. These amounts are 49–66% greater than the RDA (43). Table 1 outlines several published studies that have reported protein requirements for both endurance and strength/power athletes. In summary, optimal ...
... computed, the upper limit of the recommended protein intake was determined to be 1.33 g$kg21$d21. These amounts are 49–66% greater than the RDA (43). Table 1 outlines several published studies that have reported protein requirements for both endurance and strength/power athletes. In summary, optimal ...
Turnip yellow mosaic virus RNA can be separated into two distinct
... translate monocistronic mRNAs more readily than polycistronic ones . Up until now the existing evidence has indicated that the turnip yellow mosaic virus (TYMV) does not have a multicomponent genome. Centrifugation of TYMV in gradients of caesium chloride resolves a number of minor nucleoprotein ban ...
... translate monocistronic mRNAs more readily than polycistronic ones . Up until now the existing evidence has indicated that the turnip yellow mosaic virus (TYMV) does not have a multicomponent genome. Centrifugation of TYMV in gradients of caesium chloride resolves a number of minor nucleoprotein ban ...
AccuStart™ II Mouse Genotyping Kit
... The AccuStart II Mouse Genotyping Kit is designed for fast and easy preparation of PCR-ready DNA extracts and endpoint PCR analysis from tissues such as tail snips and ear punches commonly used for genotyping knockout and transgenic animals. The kit combines Extracta™ DNA Prep for PCR - Tissue with ...
... The AccuStart II Mouse Genotyping Kit is designed for fast and easy preparation of PCR-ready DNA extracts and endpoint PCR analysis from tissues such as tail snips and ear punches commonly used for genotyping knockout and transgenic animals. The kit combines Extracta™ DNA Prep for PCR - Tissue with ...
Mutating the second glutamate in the amidase active site
... mg/mL in 90 µL aliquots with 100 mM Tris (Fluka, USA). Substrates were added to the samples at 10-fold molar excess concentration for 30 min before denaturation. To this solution 10 µL of a mixture of 4 M urea (Sigma, USA) and 300 mM dithiotreitol (DTT) was added to a final concentration of 6 M and ...
... mg/mL in 90 µL aliquots with 100 mM Tris (Fluka, USA). Substrates were added to the samples at 10-fold molar excess concentration for 30 min before denaturation. To this solution 10 µL of a mixture of 4 M urea (Sigma, USA) and 300 mM dithiotreitol (DTT) was added to a final concentration of 6 M and ...
Final Study Guide (Semester 2) Answer Key
... CuSO4(aq) + 2KOH(aq) Cu(OH)2(s ) + K2SO4(aq) Which compounds above are strong electrolytes? CuSO4 , KOH , K2SO4 a. Which chemical above is not soluble in water? Cu(OH)2 c. Which chemical above is the precipitate? Cu(OH)2 2. Solutions of Barium nitrate and potassium sulfate are mixed. ***The first ...
... CuSO4(aq) + 2KOH(aq) Cu(OH)2(s ) + K2SO4(aq) Which compounds above are strong electrolytes? CuSO4 , KOH , K2SO4 a. Which chemical above is not soluble in water? Cu(OH)2 c. Which chemical above is the precipitate? Cu(OH)2 2. Solutions of Barium nitrate and potassium sulfate are mixed. ***The first ...
Membrane Topology of Cytochrome P450 2B4 in
... Injection of P450 2B4 beneath a DPPE monolayer caused a dramatic increase in the interfacial pressure within 60 s. A similar rapid pressure increase was seen when b5 was injected beneath DPPC monolayers. The pressure increased by as much as 1.5 mN in the first 20 s after injection at high subphase c ...
... Injection of P450 2B4 beneath a DPPE monolayer caused a dramatic increase in the interfacial pressure within 60 s. A similar rapid pressure increase was seen when b5 was injected beneath DPPC monolayers. The pressure increased by as much as 1.5 mN in the first 20 s after injection at high subphase c ...
The world of proteases Diversity and function
... Protease activity can be measured in many ways using different substrates and different reaction conditions There is not one correct way to measure protease activity An activity number is always dependent on the exact assay conditions Therefore, activity units/protease assays cannot be used to evalu ...
... Protease activity can be measured in many ways using different substrates and different reaction conditions There is not one correct way to measure protease activity An activity number is always dependent on the exact assay conditions Therefore, activity units/protease assays cannot be used to evalu ...