MPN+, a putative catalytic motif found in a subset of MPN domain
... 1). These proteins are the shortest MPN protein identified so far and most likely correspond to the structural core region of the domain. Since the finding of prokaryotic MPN proteins was highly unexpected, the validity of the assignment was confirmed by profile searches starting from the bacterioph ...
... 1). These proteins are the shortest MPN protein identified so far and most likely correspond to the structural core region of the domain. Since the finding of prokaryotic MPN proteins was highly unexpected, the validity of the assignment was confirmed by profile searches starting from the bacterioph ...
PeptidePicker: a Tool for Determining Most Appropriate Peptides for
... residues if they are predicted to yield high ion signals, even though peptides that contain cysteine or methionine are undesirable in an MRM assay because these amino acids can easily undergo chemical modification such as oxidation. This makes them "non-unique" because multiple peptide forms can be ...
... residues if they are predicted to yield high ion signals, even though peptides that contain cysteine or methionine are undesirable in an MRM assay because these amino acids can easily undergo chemical modification such as oxidation. This makes them "non-unique" because multiple peptide forms can be ...
Functional and structural roles of parasite-specific inserts in the bifunctional S-adenosylmethionine decarboxylase/ornithine
... 4.2.5.2) Dot-blot Westem analyses of SE-FPLC fractions. The collected size-exclusion chromatography fractions were transferred to nitrocellulose membranes using a BioDot apparatus (Bio-Rad) and analysed by dot blot Western. The membranes were blocked in 3% w/v low fat milk powder in lxPBS for 16 hou ...
... 4.2.5.2) Dot-blot Westem analyses of SE-FPLC fractions. The collected size-exclusion chromatography fractions were transferred to nitrocellulose membranes using a BioDot apparatus (Bio-Rad) and analysed by dot blot Western. The membranes were blocked in 3% w/v low fat milk powder in lxPBS for 16 hou ...
Exam_2005 - The University of Sydney
... Which of the following enzymes is most likely to catalyse a ‘rate limiting step’ in a pathway High Vmax enzyme that catalyses irreversible conversion of SP with the prevailing [S] being about the same as the Km of the enzyme. High Vmax enzyme that catalyses reversible conversion of SP with the pre ...
... Which of the following enzymes is most likely to catalyse a ‘rate limiting step’ in a pathway High Vmax enzyme that catalyses irreversible conversion of SP with the prevailing [S] being about the same as the Km of the enzyme. High Vmax enzyme that catalyses reversible conversion of SP with the pre ...
MODified™ Protein Domain Binding Kit Manual
... epigenetic marks play a role in the regulation of gene expression and chromatin state1-4. These histone modifications are recognized and bound by specific proteins that are coined ‘writers’ ‘readers’ and ‘erasers’. Many of these proteins contain highly conserved domains which serve as the building b ...
... epigenetic marks play a role in the regulation of gene expression and chromatin state1-4. These histone modifications are recognized and bound by specific proteins that are coined ‘writers’ ‘readers’ and ‘erasers’. Many of these proteins contain highly conserved domains which serve as the building b ...
B130_Immunohistochemical identification of PAPP-A in
... analysis of PAPP-A from atherosclerotic plaques. Assay 5H9 – 7A6 was used to detect PAPP-A/proMBP heterotetramer, whereas assay with MAbs 10A5 and 7A6 specific to PAPP-A was able to detect both – PAPP-A in PAPP-A/proMBP complex and homodimeric form of the protein. We have demonstrated the absence of ...
... analysis of PAPP-A from atherosclerotic plaques. Assay 5H9 – 7A6 was used to detect PAPP-A/proMBP heterotetramer, whereas assay with MAbs 10A5 and 7A6 specific to PAPP-A was able to detect both – PAPP-A in PAPP-A/proMBP complex and homodimeric form of the protein. We have demonstrated the absence of ...
Isoenzymes in Clinical Diagnosis
... several tissues2 have been studied for many years and have established the usefulness of serum enzyme tests. Unfortunately, many of the best studied and most easily detected enzymes occur in more than one organ. Furthermore, some organs like liver and skeletal muscle contain high concentrations of m ...
... several tissues2 have been studied for many years and have established the usefulness of serum enzyme tests. Unfortunately, many of the best studied and most easily detected enzymes occur in more than one organ. Furthermore, some organs like liver and skeletal muscle contain high concentrations of m ...
Chapter 4 Aqueous Reactions and Solution Stoichiometry
... Analyze: Our task is to write a net ionic equation for a precipitation reaction, given the names of the reactants present in solution. Plan: We first need to write the chemical formulas of the reactants and products and to determine which product is insoluble. Then we write and balance the molecular ...
... Analyze: Our task is to write a net ionic equation for a precipitation reaction, given the names of the reactants present in solution. Plan: We first need to write the chemical formulas of the reactants and products and to determine which product is insoluble. Then we write and balance the molecular ...
Reactive cysteine in proteins: Protein folding - Genoma
... on inorganic redox cofactors (NAD+, FAD, heme, Cu, Fe and other transition metals) for redox activity. In contrast, for other proteins, amino acids, mainly cysteines, are responsible for this property. Free cysteine possesses low reactivity to undergo redox transitions (Wood et al., 2003b). However, ...
... on inorganic redox cofactors (NAD+, FAD, heme, Cu, Fe and other transition metals) for redox activity. In contrast, for other proteins, amino acids, mainly cysteines, are responsible for this property. Free cysteine possesses low reactivity to undergo redox transitions (Wood et al., 2003b). However, ...
Modulation of the Oligomerization State of the Bovine F1
... Construction of Bacterial Expression Plasmids—The expression plasmid pRKIF1 contains the coding sequence for the entire bovine IF1 (11). Plasmids expressing IF1-H49K and IF1 truncated at the N terminus by 31 residues [IF1 (residues 32– 84)] were made from pRKIF1 by polymerase chain reaction using th ...
... Construction of Bacterial Expression Plasmids—The expression plasmid pRKIF1 contains the coding sequence for the entire bovine IF1 (11). Plasmids expressing IF1-H49K and IF1 truncated at the N terminus by 31 residues [IF1 (residues 32– 84)] were made from pRKIF1 by polymerase chain reaction using th ...
Fibrinogen Bern I: Substitution y 337 Asn + Lys Is
... EGTA, pH 7.4. Fragment D, (2 mg/mL) was incubated for 3 hours at 37°C with 0.3 U/mL plasminogen (Kabi) and 6 U/mL streptokinase (Kabi). The resulting fragmentsD,, D,, and D, were reduced with 2% dithiothreitol (Fluka) and separated by isoelectric focusing and sodium dodecyl sulfate (SDS) polyacrylam ...
... EGTA, pH 7.4. Fragment D, (2 mg/mL) was incubated for 3 hours at 37°C with 0.3 U/mL plasminogen (Kabi) and 6 U/mL streptokinase (Kabi). The resulting fragmentsD,, D,, and D, were reduced with 2% dithiothreitol (Fluka) and separated by isoelectric focusing and sodium dodecyl sulfate (SDS) polyacrylam ...
Chapter 3 Kinetic analysis of ribozyme cleavage
... There is an interesting array of catalytic nucleic acids known. These include a limited number of naturally occurring ribozymes, the hairpin, hammerhead, hepatitis delta virus (HDV), VS, group I and II introns, and RNase P (1). The number of ribozymes has been increased by in vitro evolution to incl ...
... There is an interesting array of catalytic nucleic acids known. These include a limited number of naturally occurring ribozymes, the hairpin, hammerhead, hepatitis delta virus (HDV), VS, group I and II introns, and RNase P (1). The number of ribozymes has been increased by in vitro evolution to incl ...
Molecular identification of three Arabidopsis thaliana mitochondrial
... In addition to respiration and cellular energy supply, plant mitochondria fulfil a range of metabolic tasks, some of which are essential to several plant-specific processes such as photorespiration, C4 and CAM (crassulacean acid metabolism) photosynthesis, the utilization of carbon, nitrogen and lip ...
... In addition to respiration and cellular energy supply, plant mitochondria fulfil a range of metabolic tasks, some of which are essential to several plant-specific processes such as photorespiration, C4 and CAM (crassulacean acid metabolism) photosynthesis, the utilization of carbon, nitrogen and lip ...
Protein kinase inhibition: natural and synthetic variations on a theme
... the conformation of the core. The physiological relevance of this mode of inhibition is also unclear since in the cell, where ATP concentrations are high, ATP may displace Tyr1158 and lead to structural instability. It is clear, however, that the adenine binding pocket can be an important ...
... the conformation of the core. The physiological relevance of this mode of inhibition is also unclear since in the cell, where ATP concentrations are high, ATP may displace Tyr1158 and lead to structural instability. It is clear, however, that the adenine binding pocket can be an important ...
Coordination and Chemistry of Stable Cu (II) Complexes in the Gas
... ligands. Data are presented for copper(II) in association with 20 different ligands, including water, ammonia, pyridine, tetrahydrofuran, and benzene. For each [Cu·Ln]2+ system, two important quantities are identified: (i) the minimum number of ligands required to form a stable unit and (ii) the val ...
... ligands. Data are presented for copper(II) in association with 20 different ligands, including water, ammonia, pyridine, tetrahydrofuran, and benzene. For each [Cu·Ln]2+ system, two important quantities are identified: (i) the minimum number of ligands required to form a stable unit and (ii) the val ...
Disallowed Ramachandran Conformations of Amino Acid Residues
... that, except in one case, main-chain–main-chain/ main-chain–side-chain/side-chain–side-chain hydrogen bonds were formed (using the criteria for N– – –O and O– – –O distance of 2.4 to 3.6 Å). The only exception was in the case of carbonoxypeptidase (5CPA) Ser57, in which the side-chain had a relativ ...
... that, except in one case, main-chain–main-chain/ main-chain–side-chain/side-chain–side-chain hydrogen bonds were formed (using the criteria for N– – –O and O– – –O distance of 2.4 to 3.6 Å). The only exception was in the case of carbonoxypeptidase (5CPA) Ser57, in which the side-chain had a relativ ...
Isr J Chem (2010) - Weizmann Institute of Science
... dynamic tunnel and in eubacteria they emerge into a shelter formed by ribosome-bound trigger-factor which acts as a “chaperone” to prevent aggregation and misfolding. The peptidyl transferase center (PTC) is the primary catalytic core of the ribosome. It is responsible for promoting the chemical cat ...
... dynamic tunnel and in eubacteria they emerge into a shelter formed by ribosome-bound trigger-factor which acts as a “chaperone” to prevent aggregation and misfolding. The peptidyl transferase center (PTC) is the primary catalytic core of the ribosome. It is responsible for promoting the chemical cat ...
Biological Membrane Structure By Solid-State NMR
... restricted molecular motions result in broad NMR spectra. This contribution will first present an overview of the different techniques used to study lipid bilayers, namely 31P, 2H and 13C solid-state NMR spectroscopy. On the other hand, the study of the structure of membrane peptides and proteins is ...
... restricted molecular motions result in broad NMR spectra. This contribution will first present an overview of the different techniques used to study lipid bilayers, namely 31P, 2H and 13C solid-state NMR spectroscopy. On the other hand, the study of the structure of membrane peptides and proteins is ...
The First Steps of Chemical Evolution towards the
... know that the first fossile cells have been assigned an age of 3.5 billion years [3], which means that chemical evolution leading from the first organic molecules to the first celllike structures took not more than 300 million years. About the atmosphere of the primitive earth, many new findings hav ...
... know that the first fossile cells have been assigned an age of 3.5 billion years [3], which means that chemical evolution leading from the first organic molecules to the first celllike structures took not more than 300 million years. About the atmosphere of the primitive earth, many new findings hav ...
Physical and chemical interactions between aphids and plants
... phytopathogens are more likely functions of sieve plate plugging. The lectin character of the structural phloem protein PP2 in Cucurbita (Read and Northcote, 1983), which takes part in forming sieve plate plugs, also suggests that sealing acts a barrier against bacteria, viruses, and fungi. This att ...
... phytopathogens are more likely functions of sieve plate plugging. The lectin character of the structural phloem protein PP2 in Cucurbita (Read and Northcote, 1983), which takes part in forming sieve plate plugs, also suggests that sealing acts a barrier against bacteria, viruses, and fungi. This att ...
Euglena gracilis Rhodoquinone:Ubiquinone Ratio and
... In-gel Digestion and ESI-Q-TOF-MS/MS Analysis—Protein spots of interest were cut from the gel, washed twice with 50% (v/v) acetonitrile, and incubated successively with 100% acetonitrile, 100 mM NH4HCO3, and 100 mM 1:1 NH4HCO3:acetonitrile. After vacuum drying, the gel pieces were reswollen with 10 ...
... In-gel Digestion and ESI-Q-TOF-MS/MS Analysis—Protein spots of interest were cut from the gel, washed twice with 50% (v/v) acetonitrile, and incubated successively with 100% acetonitrile, 100 mM NH4HCO3, and 100 mM 1:1 NH4HCO3:acetonitrile. After vacuum drying, the gel pieces were reswollen with 10 ...
MS Word
... Basal GTP binding and hydrolysis (K1, k2 and K1’, k2’). The chemical step is rate-limiting for the basal GTPase reaction of cpSRP54, because the maximal rate constant of GTP hydrolysis (0.017 min-1; Figure 2A) is 4 104 -fold slower than the rate at which GTP dissociates from the enzyme active site ...
... Basal GTP binding and hydrolysis (K1, k2 and K1’, k2’). The chemical step is rate-limiting for the basal GTPase reaction of cpSRP54, because the maximal rate constant of GTP hydrolysis (0.017 min-1; Figure 2A) is 4 104 -fold slower than the rate at which GTP dissociates from the enzyme active site ...
ResearchBlurb_JoVE_v2 - Stanford Microfluidics Laboratory
... level governed by the properties of the initial LE mixture. As they focus, sample ions in plateau mode also displace each other and separate into adjoining pure zones. ...
... level governed by the properties of the initial LE mixture. As they focus, sample ions in plateau mode also displace each other and separate into adjoining pure zones. ...
Comparison of the Structure of the Extrinsic 33 kDa Protein from
... directly by a MALDI-TOF mass spectrometer in order to determine their cleavage sites. Since whether a peptide fragment can be detected by the MALDI-TOF mass spectrometer depends on the matrix employed and the molecular mass of that fragment, two different matrices were used. One was a mixture of sin ...
... directly by a MALDI-TOF mass spectrometer in order to determine their cleavage sites. Since whether a peptide fragment can be detected by the MALDI-TOF mass spectrometer depends on the matrix employed and the molecular mass of that fragment, two different matrices were used. One was a mixture of sin ...
Protein relative abundance patterns associated with sucrose
... found that healthy oral sites from different people were broadly similar with respect to gene function, even though there was an extensive individual variation in their taxonomic profiles. That pattern may also extend to dysbiotic communities. In that case, shifts in community-wide protein relative ...
... found that healthy oral sites from different people were broadly similar with respect to gene function, even though there was an extensive individual variation in their taxonomic profiles. That pattern may also extend to dysbiotic communities. In that case, shifts in community-wide protein relative ...