(NH 3 ) 2 - GZ @ Science Class Online
... Carry out procedures to identify ions involves collecting primary data and using these observations to identify ions in a solution using a procedure provided. Identification of ions must be supported by experimental observations and identification of all precipitates formed. Ions to be identified wi ...
... Carry out procedures to identify ions involves collecting primary data and using these observations to identify ions in a solution using a procedure provided. Identification of ions must be supported by experimental observations and identification of all precipitates formed. Ions to be identified wi ...
Enzyme from Banana (Musa sp.) Extraction Procedures for Sensitive
... total protein concentration for the CEE with and without the cleaning step. This table also shows the specific activities, defined as enzyme activity divided by total protein concentration [35]. The second procedure (maceration) showed ca. 50% lower enzyme activity than the grinding in a blender (pr ...
... total protein concentration for the CEE with and without the cleaning step. This table also shows the specific activities, defined as enzyme activity divided by total protein concentration [35]. The second procedure (maceration) showed ca. 50% lower enzyme activity than the grinding in a blender (pr ...
IMMUNOCHEMICAL TECHNIQUES Antigens Antibodies
... of their mutual immunochemical relationships if the specific antisera are available. On the other hand, this method may be used for characterisation of antibodies using known purified antigens. ...
... of their mutual immunochemical relationships if the specific antisera are available. On the other hand, this method may be used for characterisation of antibodies using known purified antigens. ...
TD12 Characterization of DnaJ substrate specificity Reference
... G/E domain- contributes to stimulation of ATP hydrolysis (of DnaK) Zn binding site, amino acid ~144 C-terminal domain- amino acids 144-376, binds protein substrates Model: DnaJ first binds to unfolded protein substrates, and then hands them over to DnaK, triggering ATP hydrolysis as it delivers subs ...
... G/E domain- contributes to stimulation of ATP hydrolysis (of DnaK) Zn binding site, amino acid ~144 C-terminal domain- amino acids 144-376, binds protein substrates Model: DnaJ first binds to unfolded protein substrates, and then hands them over to DnaK, triggering ATP hydrolysis as it delivers subs ...
Mechanisms and applications of disulfide bond formation
... containing proteins in the cytoplasm of prokaryotes. Specifically, to examine in more detail the mechanism of action of PDI in catalyzing folding of disulfide bond-containing proteins a fluorescence screening of 75 mutants of the b’x domain was carried out and mutants that stabilize the b’x domain i ...
... containing proteins in the cytoplasm of prokaryotes. Specifically, to examine in more detail the mechanism of action of PDI in catalyzing folding of disulfide bond-containing proteins a fluorescence screening of 75 mutants of the b’x domain was carried out and mutants that stabilize the b’x domain i ...
Solution Structure of the Tandem Acyl Carrier Protein Domains from
... (ACP) domains in tandem. This conserved tandem arrangement has been implicated in both amplification of fatty acid production (additive effect) and in structural stabilization of the multidomain protein (synergistic effect). While the more accepted model is one in which domains act independently, re ...
... (ACP) domains in tandem. This conserved tandem arrangement has been implicated in both amplification of fatty acid production (additive effect) and in structural stabilization of the multidomain protein (synergistic effect). While the more accepted model is one in which domains act independently, re ...
A defined subset of adenylyl cyclases is regulated by bicarbonate ion.
... determine whether HCO3 - up regulated cyaB1595-859 specific activity by increasing homodimer formation we examined the ratio of the HCO3- and Cl- specific activities as a function of protein concentration. Interestingly, this ratio remained constant over the range of protein concentrations tested in ...
... determine whether HCO3 - up regulated cyaB1595-859 specific activity by increasing homodimer formation we examined the ratio of the HCO3- and Cl- specific activities as a function of protein concentration. Interestingly, this ratio remained constant over the range of protein concentrations tested in ...
Case Study: BPTI
... Most globular proteins are stabilized by their hydrophobic core which is formed by the internal packing of non-polar amino acid side chains. BPTI is no exception and due to its rather small overall size it also has a relatively small hydrophobic core. Thus, extra stability to support its three dimen ...
... Most globular proteins are stabilized by their hydrophobic core which is formed by the internal packing of non-polar amino acid side chains. BPTI is no exception and due to its rather small overall size it also has a relatively small hydrophobic core. Thus, extra stability to support its three dimen ...
Fingerprinting the Fungal Community
... from whatever other organisms are present in the sample. ...
... from whatever other organisms are present in the sample. ...
Hydrolases as Catalysts for Green Chemistry and
... The use of enzymes in industrial applications has been recognised for providing clean processes with minimal impact on the environment. This thesis presents studies on engineering of enzymes and enzymebased processes in the light of green chemistry and environmental sustainability, and focuses on th ...
... The use of enzymes in industrial applications has been recognised for providing clean processes with minimal impact on the environment. This thesis presents studies on engineering of enzymes and enzymebased processes in the light of green chemistry and environmental sustainability, and focuses on th ...
Lactococcus lactis as expression host for the biosynthetic
... protein chemistry [1,2]. Its emission maximum (λmax ) and the quantum yield (Q) are highly sensitive parameters for monitoring changes in the structure and dynamics of a protein. In numerous studies, the intrinsic protein fluorescence was used to monitor processes like enzyme kinetics, protein–ligan ...
... protein chemistry [1,2]. Its emission maximum (λmax ) and the quantum yield (Q) are highly sensitive parameters for monitoring changes in the structure and dynamics of a protein. In numerous studies, the intrinsic protein fluorescence was used to monitor processes like enzyme kinetics, protein–ligan ...
A Low-Fluorine Solution with the F/Ba Mole Ratio of 2 for the
... processed YBCO film. It can be observed that this sample is composed of plate-like c-axis oriented grains with a small amount of nanoscale pores and Cu-rich surface particles. The domination of the c-axis oriented grains could be confirmed by XRD θ/2θ characterization, as shown in Figure 3(b). The i ...
... processed YBCO film. It can be observed that this sample is composed of plate-like c-axis oriented grains with a small amount of nanoscale pores and Cu-rich surface particles. The domination of the c-axis oriented grains could be confirmed by XRD θ/2θ characterization, as shown in Figure 3(b). The i ...
Use of a Sec signal peptide library from Bacillus subtilis for the
... machinery. For example, >150 Sec-specific SPs have been identified for Bacillus subtilis alone. As the best SP for a target protein of choice cannot be predicted a priori, screening of homologous SPs has been shown to be a powerful tool for different expression organisms. While SP libraries between ...
... machinery. For example, >150 Sec-specific SPs have been identified for Bacillus subtilis alone. As the best SP for a target protein of choice cannot be predicted a priori, screening of homologous SPs has been shown to be a powerful tool for different expression organisms. While SP libraries between ...
Kinetic Role of Helix Caps in Protein Folding Is Context
... of the dramatic effect of C-cap glycine expected from its frequent occurrence in this structural motif (7, 23). This difference could be related to whether the C-cap is at the chain terminus, but that relationship has not been previously investigated, and the peptide results have apparently discoura ...
... of the dramatic effect of C-cap glycine expected from its frequent occurrence in this structural motif (7, 23). This difference could be related to whether the C-cap is at the chain terminus, but that relationship has not been previously investigated, and the peptide results have apparently discoura ...
Carbamoyl Phosphate Synthetase: Closure of the B
... between Leu 650 to Ile 775. Following this refinement cycle, the R-factor decreased to 35.0%. With over 5800 amino acid residues in the asymmetric unit, the goal of the modelbuilding process was to lower the R-factor as much as possible using an “averaged” R,β-heterodimer before finally rebuilding t ...
... between Leu 650 to Ile 775. Following this refinement cycle, the R-factor decreased to 35.0%. With over 5800 amino acid residues in the asymmetric unit, the goal of the modelbuilding process was to lower the R-factor as much as possible using an “averaged” R,β-heterodimer before finally rebuilding t ...
Book Review Werner Callebaut and Diego Rasskin
... Part IV of the book (“Modularity of Mind and Culture”) takes us into a different realm. The first three contributions deal with modularity of mind. In the first Calabretta and Parisi discuss the contention between connectionism and modularism. They describe two artificial life simulations where modu ...
... Part IV of the book (“Modularity of Mind and Culture”) takes us into a different realm. The first three contributions deal with modularity of mind. In the first Calabretta and Parisi discuss the contention between connectionism and modularism. They describe two artificial life simulations where modu ...
Biosynthesis of Glucosyl Glycerol, a Compatible Solute, Using
... Expression of pETMFAS and the Purification of the Recombinant MFAS Recombinant E. coli BL21 harboring pETMFAS were grown in 1 L of LB medium supplemented with ampicillin (0.1 mg/mL) at 37 °C with agitation. The cells were harvested by centrifugation at 4 °C after a 3-h induction of the mfas gene by ...
... Expression of pETMFAS and the Purification of the Recombinant MFAS Recombinant E. coli BL21 harboring pETMFAS were grown in 1 L of LB medium supplemented with ampicillin (0.1 mg/mL) at 37 °C with agitation. The cells were harvested by centrifugation at 4 °C after a 3-h induction of the mfas gene by ...
T. Takahashi, B. C. Vo Ngo, L. Xiao, G. Arya, and M. J. Heller
... While considerable attempts have been made to recreate the high turnover rates of enzymes using synthetic enzyme mimics, most have failed and only a few have produced minimal reaction rates that can barely be considered catalytic. One particular approach we have focused on is the use of short-sequen ...
... While considerable attempts have been made to recreate the high turnover rates of enzymes using synthetic enzyme mimics, most have failed and only a few have produced minimal reaction rates that can barely be considered catalytic. One particular approach we have focused on is the use of short-sequen ...
PDF Full-text
... 1. Introduction Silk, which is secreted by silkworms, spiders, mites, and pseudo-scorpions, is a generally fibrous protein that has attracted considerable attention due to its inherent optical and outstanding mechanical properties [1–4]. It has been widely used in high quality textile industries, bu ...
... 1. Introduction Silk, which is secreted by silkworms, spiders, mites, and pseudo-scorpions, is a generally fibrous protein that has attracted considerable attention due to its inherent optical and outstanding mechanical properties [1–4]. It has been widely used in high quality textile industries, bu ...
Diagnostic protocol for
... will fluoresce bright green under the ultraviolet light of the microscope. If the positive control with known bacterium fluoresces and the negative controls of normal serum and PBS do not, examine the sample windows for bacterial cell wall fluorescence, looking for the cells with the size and form o ...
... will fluoresce bright green under the ultraviolet light of the microscope. If the positive control with known bacterium fluoresces and the negative controls of normal serum and PBS do not, examine the sample windows for bacterial cell wall fluorescence, looking for the cells with the size and form o ...
KHARKOV STATE MEDICAL UNIVERSITY
... externally in otolaryngology, stomatology and ophthalmology. It may be used for neutralization of acids got on the mucosas and integuments. Hydrogen is one of six elements – organogens (C, H, O, N, P, S), which constitute 97,4% of human body mass. Hydrogen makes up about 10 % of human body mass. Hyd ...
... externally in otolaryngology, stomatology and ophthalmology. It may be used for neutralization of acids got on the mucosas and integuments. Hydrogen is one of six elements – organogens (C, H, O, N, P, S), which constitute 97,4% of human body mass. Hydrogen makes up about 10 % of human body mass. Hyd ...
Urine Sediment Guide
... the urine, proteinuria can indicate both renal and nonrenal disease. If significant proteinuria is detected and there is an inactive sediment, urine protein:creatinine ratio (UPC) should be performed for protein quantification for accurate assessment and monitoring. ...
... the urine, proteinuria can indicate both renal and nonrenal disease. If significant proteinuria is detected and there is an inactive sediment, urine protein:creatinine ratio (UPC) should be performed for protein quantification for accurate assessment and monitoring. ...
PPTX - Bonham Chemistry
... To perform a step mash with an acid rest: – Add hot water to your grain bill to achieve an appropriate rest temperature for an acid rest. Hold the mash at this temperature until the proper pH is achieved . Note this may take more than one hour. – Add hot water infusion or direct heat to raise the ma ...
... To perform a step mash with an acid rest: – Add hot water to your grain bill to achieve an appropriate rest temperature for an acid rest. Hold the mash at this temperature until the proper pH is achieved . Note this may take more than one hour. – Add hot water infusion or direct heat to raise the ma ...
TD Sheet - Eu-anti-P-MBP-Draft 0.1
... LIMITED WARRANTY: PerkinElmer BioSignal Inc., a subsidiary of PerkinElmer Life and Analytical Sciences, warrants that, at the time of shipment, the products sold by it are free from defects in material and workmanship and conform to specifications which accompany the product. PerkinElmer BioSignal I ...
... LIMITED WARRANTY: PerkinElmer BioSignal Inc., a subsidiary of PerkinElmer Life and Analytical Sciences, warrants that, at the time of shipment, the products sold by it are free from defects in material and workmanship and conform to specifications which accompany the product. PerkinElmer BioSignal I ...
a curated database for protein phosphorylation sites in prokaryotes
... its critical roles in various cellular processes such as signal transduction. Thus, an integrative data resource of the prokaryotic phosphorylation will be useful for further analysis. In this study, we presented a curated database of phosphorylation sites in prokaryotes (dbPSP, Database URL: http:/ ...
... its critical roles in various cellular processes such as signal transduction. Thus, an integrative data resource of the prokaryotic phosphorylation will be useful for further analysis. In this study, we presented a curated database of phosphorylation sites in prokaryotes (dbPSP, Database URL: http:/ ...