Bacillus cereus
... and produces a large variation of enzymes. This species is good cloning host because it is able to house numerous plasmid vectors while remaining stable due to its unique external proteases. The organism does not have alkaline proteases; which allows for recombinant protein synthesis. Using Bacillus ...
... and produces a large variation of enzymes. This species is good cloning host because it is able to house numerous plasmid vectors while remaining stable due to its unique external proteases. The organism does not have alkaline proteases; which allows for recombinant protein synthesis. Using Bacillus ...
Ionic Compound Solubility Nitrates (NO3 ) Ionic Compound
... Definition A substance that is able to donate a H+ ion (a proton) and, hence, increases the concentration of H+(aq) when it dissolves in water. ...
... Definition A substance that is able to donate a H+ ion (a proton) and, hence, increases the concentration of H+(aq) when it dissolves in water. ...
Structure and Function of Mammalian Ribosomes
... the gradient (Noll, 1969). In low salt gradient conditions free 80 s couples as well as polysomes are stable. In high salt gradient conditions only polysomes and 80 s ribosomes still attached to mRNA snd carrying a peptidyl-tRNA are stable, whereas free 80 s couples dissociate into subunits. Samples ...
... the gradient (Noll, 1969). In low salt gradient conditions free 80 s couples as well as polysomes are stable. In high salt gradient conditions only polysomes and 80 s ribosomes still attached to mRNA snd carrying a peptidyl-tRNA are stable, whereas free 80 s couples dissociate into subunits. Samples ...
- Free Documents
... both cases it is the gain or loss oI phosphate groups that determines whether the protein is active or inactive. The largest class consists oI proteins that are activated or inactivated by . For these proteins, the switch is thrown in one Chapter phosphorylation discussed in direction by a protein k ...
... both cases it is the gain or loss oI phosphate groups that determines whether the protein is active or inactive. The largest class consists oI proteins that are activated or inactivated by . For these proteins, the switch is thrown in one Chapter phosphorylation discussed in direction by a protein k ...
Phosphorylation of the F1Fo ATP Synthase Я Subunit
... nonphosphorylatable strain (T262A) had an ATPase rate equivalent to wild type. Although T262E, like all of the  subunit mutants, was able to form the intact complex (F1Fo), this strain lacked a free F1 component found in wild-type and had a corresponding increase of lower-molecular-weight forms of ...
... nonphosphorylatable strain (T262A) had an ATPase rate equivalent to wild type. Although T262E, like all of the  subunit mutants, was able to form the intact complex (F1Fo), this strain lacked a free F1 component found in wild-type and had a corresponding increase of lower-molecular-weight forms of ...
Chapter 4 Aqueous Reactions and Solution Stoichiometry
... Zn (s) + 2 CuNO3 (aq) 2 Cu (s) + Zn(NO3)2 (aq) Cu (s) + 2 AgNO3 (aq) 2 Ag (s) + Cu(NO3)2 (aq) HCl (aq) + NaOH (aq) NaCl (aq) + H2O (l) ...
... Zn (s) + 2 CuNO3 (aq) 2 Cu (s) + Zn(NO3)2 (aq) Cu (s) + 2 AgNO3 (aq) 2 Ag (s) + Cu(NO3)2 (aq) HCl (aq) + NaOH (aq) NaCl (aq) + H2O (l) ...
ref. #27 of the TIBS article
... a-Helix formation in globular proteins has been studied both theoretically and experimentally for decades, while a lack of both high-resolution structures and suitable experimental techniques has hampered the study of helices in membrane proteins. We have developed a new experimental approach, glyco ...
... a-Helix formation in globular proteins has been studied both theoretically and experimentally for decades, while a lack of both high-resolution structures and suitable experimental techniques has hampered the study of helices in membrane proteins. We have developed a new experimental approach, glyco ...
Conformational changes in actin–myosin isoforms probed by Ni(II
... Crucial information concerning conformational changes that occur during the mechanochemical cycle of actin– myosin complexes is lacking due to the difficulties encountered in obtaining their three-dimensional structures. To obtain such information, we employed a solution-based approach through the re ...
... Crucial information concerning conformational changes that occur during the mechanochemical cycle of actin– myosin complexes is lacking due to the difficulties encountered in obtaining their three-dimensional structures. To obtain such information, we employed a solution-based approach through the re ...
K 2
... demonstrated that strain with capsule non K1/K2 isolates and only one in K2 such as (K1, K2) were virulent to serotypes, human. whereas serotypes without through using specific primer . capsule are less virulent or without Although this gene is high important virulent. for Klebsiella pneumonia which ...
... demonstrated that strain with capsule non K1/K2 isolates and only one in K2 such as (K1, K2) were virulent to serotypes, human. whereas serotypes without through using specific primer . capsule are less virulent or without Although this gene is high important virulent. for Klebsiella pneumonia which ...
Single Processing Center Models for Human Dicer and Bacterial
... contains two domains related to the bacterial dsRNAspecific endonuclease, RNase III, which is known to function as a homodimer. Based on an X-ray structure of the Aquifex aeolicus RNase III, models of the enzyme interaction with dsRNA, and its cleavage at two composite catalytic centers, have been p ...
... contains two domains related to the bacterial dsRNAspecific endonuclease, RNase III, which is known to function as a homodimer. Based on an X-ray structure of the Aquifex aeolicus RNase III, models of the enzyme interaction with dsRNA, and its cleavage at two composite catalytic centers, have been p ...
... than fins, and their hydrolysates had the highest antioxidant activity. Also, jumbo squid arm-derived collagen hydrolyzed with protease XIV showed the highest antimutagenic activity. The four hydrolysates obtained showed low antiproliferative activity, however they are susceptible for further studie ...
Abstract Background The present study aimed to compare the
... components of ginseng mainly include ginsenosides, polysaccharides, fatty acids, and amino acids [10]. A recent study showed that the amino acids of ginseng are candidate therapeutic agents with antidepressant, blood pressure reduction, immunity strengthening, and myocardium- and liver-protective ac ...
... components of ginseng mainly include ginsenosides, polysaccharides, fatty acids, and amino acids [10]. A recent study showed that the amino acids of ginseng are candidate therapeutic agents with antidepressant, blood pressure reduction, immunity strengthening, and myocardium- and liver-protective ac ...
Non-ideal Plastic Behavior
... products to have standardized nutritional labels. One of the major reasons for introducing these regulations was so that consumers could make informed choices about their diet. Nutritional labels state the total calorific value of the food, as well as total fat, saturated fat, cholesterol, sodium, c ...
... products to have standardized nutritional labels. One of the major reasons for introducing these regulations was so that consumers could make informed choices about their diet. Nutritional labels state the total calorific value of the food, as well as total fat, saturated fat, cholesterol, sodium, c ...
Further studies on the new coomassie brilliant blue G-250 - K-REx
... dye was observed for different pH values against a distilled water blank. In the absence of any added base the visible spectrum of the dye shows a maximum in the 460 nm range, a valley in the 585-590 region and another ...
... dye was observed for different pH values against a distilled water blank. In the absence of any added base the visible spectrum of the dye shows a maximum in the 460 nm range, a valley in the 585-590 region and another ...
Intestinal peptidases form functional complexes with the neutral
... where proteins are hydrolysed into small peptides (2–10 amino acids long) by intestinal proteases. Subsequent digestion occurs via the membrane-bound brush-border peptidases, which further hydrolyse small oligopeptides to produce di-/tri-peptides and single amino acids [3,4]. These digestion end-pro ...
... where proteins are hydrolysed into small peptides (2–10 amino acids long) by intestinal proteases. Subsequent digestion occurs via the membrane-bound brush-border peptidases, which further hydrolyse small oligopeptides to produce di-/tri-peptides and single amino acids [3,4]. These digestion end-pro ...
1. INTRODUCTION The dried cells of
... proteins, biochemicals, lipids, polysaccharides and colorants (Athukorala et al., 2006). Among microalgal species, Spirulina utilized as food in the area around Lake Chad for long time ago and marketed as a healthy aliment in the United States and Japan (Borowizka, 1998). Spirulina (Arthrospira) is ...
... proteins, biochemicals, lipids, polysaccharides and colorants (Athukorala et al., 2006). Among microalgal species, Spirulina utilized as food in the area around Lake Chad for long time ago and marketed as a healthy aliment in the United States and Japan (Borowizka, 1998). Spirulina (Arthrospira) is ...
Amino Acid Analysis Amino acid analysis refers to the methodology
... glassware used for hydrolysis must be very clean to avoid erroneous results. Glove powders and fingerprints on hydrolysis tubes may cause contamination. To clean glass hydrolysis tubes, boil tubes for 1 hour in 1 M hydrochloric acid or soak tubes in concentrated nitric acid or in a mixture of equal ...
... glassware used for hydrolysis must be very clean to avoid erroneous results. Glove powders and fingerprints on hydrolysis tubes may cause contamination. To clean glass hydrolysis tubes, boil tubes for 1 hour in 1 M hydrochloric acid or soak tubes in concentrated nitric acid or in a mixture of equal ...
Supporting Information - Royal Society of Chemistry
... Figure S2. Changes in the absorption maxima are plotted against the ratio of enzyme to complex 3. The dotted line is connecting the data points; it is not a fitted curve. Inhibition data for the complexes: For determining the Ki values of complexes 2-4, the concentrations of different compounds requ ...
... Figure S2. Changes in the absorption maxima are plotted against the ratio of enzyme to complex 3. The dotted line is connecting the data points; it is not a fitted curve. Inhibition data for the complexes: For determining the Ki values of complexes 2-4, the concentrations of different compounds requ ...
ARIUS MACULATUS EAST COAST OF INDIA
... Stargazers and some species of Shark, Ratfish, Catfish, Surgeonfish and Blenny are known or suspected to be venomous [1]. The production of toxins by aquatic animals is an important strategy that guarantees its survival in a highly competitive ecosystem. In this way, these animals defend themselves ...
... Stargazers and some species of Shark, Ratfish, Catfish, Surgeonfish and Blenny are known or suspected to be venomous [1]. The production of toxins by aquatic animals is an important strategy that guarantees its survival in a highly competitive ecosystem. In this way, these animals defend themselves ...
as a PDF - CiteSeerX
... activity assay, the activation appeared to be complete within a few min (Fig. 2). The formation of the intermediate form became predominant when the initial pepsinogen concentration decreased. Released peptides were also detected as two bands. The amount of peptide in the high molecular weight pepti ...
... activity assay, the activation appeared to be complete within a few min (Fig. 2). The formation of the intermediate form became predominant when the initial pepsinogen concentration decreased. Released peptides were also detected as two bands. The amount of peptide in the high molecular weight pepti ...
Progesterone-stimulated intracellular calcium increase in human
... 1988) and further incubated for 15 min. [Ca21]i was measured as previously described using a spectrofluorimetric method (Baldi et al., 1991; Falsetti et al., 1993). Spermatozoa were transferred to a quartz cuvette in a total volume of 2 ml containing a total of 53106 spermatozoa. Fluorescence was me ...
... 1988) and further incubated for 15 min. [Ca21]i was measured as previously described using a spectrofluorimetric method (Baldi et al., 1991; Falsetti et al., 1993). Spermatozoa were transferred to a quartz cuvette in a total volume of 2 ml containing a total of 53106 spermatozoa. Fluorescence was me ...
Ni recovery using KOH, NaOH, and NH4OH in the presence of
... constants. Thermodynamically, the most preferred metal to be complexed is Ni, followed by Cu, Zn, or Pb. EDTA is able to solubilize heavy metal ions bonded onto sedimented solids. Therefore the complexity of the EDTA-metal interactions depends on metal concentrations, pH, and nature of the sediment ...
... constants. Thermodynamically, the most preferred metal to be complexed is Ni, followed by Cu, Zn, or Pb. EDTA is able to solubilize heavy metal ions bonded onto sedimented solids. Therefore the complexity of the EDTA-metal interactions depends on metal concentrations, pH, and nature of the sediment ...
Introduction to NMR spectroscopy
... size expressed in terms of tumbling (correlation) time -> there is an experimental limit to the size of the proteins one can determine by NMR ! we need tricks, more tricks.... ...
... size expressed in terms of tumbling (correlation) time -> there is an experimental limit to the size of the proteins one can determine by NMR ! we need tricks, more tricks.... ...
Shigella boydii Involved in O-antigen Synthesis By
... serotype B14 consists of repeating units with the structure [6-D-Galp14-DGlcpA16-D-Galp14-D-Galp14-D-GlcpNAc1]n . None of the genes in the S. boydii serotype B14 O-antigen gene cluster, which includes putative GT genes, have been functionally characterized. We have developed the technolog ...
... serotype B14 consists of repeating units with the structure [6-D-Galp14-DGlcpA16-D-Galp14-D-Galp14-D-GlcpNAc1]n . None of the genes in the S. boydii serotype B14 O-antigen gene cluster, which includes putative GT genes, have been functionally characterized. We have developed the technolog ...