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Glycoblocks: a schematic three-dimensional
Glycoblocks: a schematic three-dimensional

... Visualizing interactions with Glycoblocks. In the figure, the structure of a heavily glycosylated fungal glycosylhydrolase (PDB code 5fjj), reported by Agirre et al. (2016). (a) View of the interactions of a high-mannose tree. The glycan connected to Asn323 is perhaps the only example of a threedime ...
Grafting Techniques towards Production of Peptide-Tethered
Grafting Techniques towards Production of Peptide-Tethered

... size [18–21]. Moreover, peptides are generally biocompatible and do not cause severe immune responses, particularly those with smaller sequences, as they are composed of naturally occurring or metabolically degradable amino acids. In general, the compositional homology between peptide drug candidate ...
Adjeitey_Cyril _Nii-Klu_2013_ thesis
Adjeitey_Cyril _Nii-Klu_2013_ thesis

... UCP1 is a member of the mitochondrial transmembrane anion carrier protein superfamily and is required to mediate adaptive thermogenesis in brown adipose tissue (BAT). Once activated, UCP1 uncouples mitochondrial respiration from ATP synthesis, thereby wasting the protonmotive force formed across the ...
Structural Studies on Sulfated Glycopeptides from the Carbohydrate
Structural Studies on Sulfated Glycopeptides from the Carbohydrate

... due which is remote from the innermost xylose. This new structure was confirmed by two-dimensional hom- region. The high field ‘H NMR spectra of the linkage glycoonuclear Hartmann-Hahn spectroscopy. The molar ra-peptides isolated from rat chondrosarcoma proteoglycans retio of the isolated nonsulfate ...
12. Molecular Recognition: The Thermodynamics of
12. Molecular Recognition: The Thermodynamics of

... From The Molecules of Life by Kuriyan, Konforti & Wemmer © Garland Publishing, 2009. Distribution Prohibited. ...
Amino Acids
Amino Acids

... • Collagen & elastin are examples of common, wellcharacterized fibrous proteins that serve structural functions in the body • E.g., collagen & elastin found as components in skin, connective tissue, blood vessel walls, & sclera and cornea of eye. • Each fibrous protein exhibits special mechanical pr ...
File
File

... 7. Effect of pH on Enzymes Enzymes work best within a range of pH depending on the type of enzyme. The pH that the enzymes works best at is called it’s Optimum pH If the pH is too high, the enzymes active site changes shape (denatured) What is the optimum pH of this enzyme ? ...
Modulation of functional properties of bifunctional S- Adenosylmethionine decarboxylase/Ornithine decarboxylase of
Modulation of functional properties of bifunctional S- Adenosylmethionine decarboxylase/Ornithine decarboxylase of

... Trishydroxy (methyl-amino) methane / Hydrochloric acid ...
A tribute to sulfur - Wiley Online Library
A tribute to sulfur - Wiley Online Library

... was found that the active site Cys residue is located in the middle of a highly flexible loop of 12 amino acids, which may be able to adapt to varied geometries in acceptor proteins. The 3D structure of C-DES from Synechocystis has now also become available [52]. Contrary to the NifS type Cys desulf ...
(9E10): sc-40 - Santa Cruz Biotechnology
(9E10): sc-40 - Santa Cruz Biotechnology

... carcinomas. The presence of three sequence motifs in the c-Myc COOH terminus, including the leucine zipper, the helix-loop-helix and a basic region, provided initial evidence for a sequence-specific binding function. A basic region helix-loop-helix leucine zipper motif (bHLH-Zip) protein, designated ...
Efficient Phosphodiester Hydrolysis by
Efficient Phosphodiester Hydrolysis by

... model substrate BDNPP were monitored spectrophotometrically for the absorbance increase at 400 nm due to the formation of 2,4-dinitrophenolate over time, under conditions of excess complex at 25 °C, and the reaction mixtures were monitored until saturation was reached. In these experiments, all of t ...
Microsoft Word
Microsoft Word

... specificity [17]. Replacement of two amino acids, N64Y/V65L decreased the size of the pocket and gave rise to discrimination against TMG-cap by steric hindrance. However, it was noted that dual specific A. suum eIF4E does contain Y64 and L65 residues [18]. Unfortunately, discrimination between TMG-c ...
Supplemental Methods
Supplemental Methods

... 75% ACN in PBS/CHAPS per well. The total elapsed time during the wash steps was approximately 10 min (i.e., shorter than the half off-time of the anti-peptide antibodies). Finally, the bead-antibody complex reached the elution plate where the target peptides were eluted in 13 L of 0.1% formic acid. ...


... (SDS-PAGE) Tricine SDS-PAGE was carried out according to the procedure of Schägger & von Jagow (1987) with some modifications. The separating gel, spacer gel, and stacking gel were well prepared and solidified. For supernatant, the highest protein concentration sample was diluted to 1mg/mL. Precipit ...
pdf-1 - The Wolfson Centre for Applied Structural Biology
pdf-1 - The Wolfson Centre for Applied Structural Biology

... serum. Produced as part of the secondary immune response to an antigen, this class of immunoglobulin constitutes approximately 75% of total serum Ig. IgG is the only class of Ig that can cross the placenta in humans, and it is largely responsible for protection of the newborn during the first months ...
Linear Polymer
Linear Polymer

... • Structural: e.g. collagen in teehth and bone; keratin in skin, hair and nails. • Contractile proteins: actin and myosin in muscles allow contraction and therefore movement. • Hormones: many hormones have a protein structure (e.g. insulin, glucagon, growth hormone). • Transport: for example, haemog ...
Immersive Projection for Biochemical Pattern Matching
Immersive Projection for Biochemical Pattern Matching

... turn stabilises to form the secondary structure (alpha helixes or beta sheets). This stabilised structure then folds to form the tertiary structure, the fully folded, stable state of the protein. The interactions involved in stabilising this folded state of the protein primarily consist of weak van ...
Fluorescent Reporter Proteins for the Tonoplast
Fluorescent Reporter Proteins for the Tonoplast

... plants expressing the RFP-based reporters (Fig. 1). In mature leaves, spRFP was exclusively detected in the apoplast (Fig. 2, A and B), as previously observed in tobacco epidermal cells (Zheng et al., 2005). In contrast, spRFP-AFVY and spL-RFP were both localized exclusively in the lumina of the lar ...
1aUnit Two Handouts - Dunmore High School
1aUnit Two Handouts - Dunmore High School

... A sodium hydroxide solution is added to a phosphoric acid solution. Assume that 2 of the hydrogens in phosphoric acid react. ...
UNIFORM PARTICLES WITH A LARGE SURFACE AREA FORMED
UNIFORM PARTICLES WITH A LARGE SURFACE AREA FORMED

... product, is rather difficult. In contrast, the properties of samples of the ferric gel prepared by hydrolysis with urea were surprisingly different in many aspects. The sedimentation of the precipitates in the aqueous media was very rapid, their filtration and washing was easy, and after drying, the ...
Sequence and Tissue Distribution of a Second Protein of Hepatic
Sequence and Tissue Distribution of a Second Protein of Hepatic

... were then exposed to XAR-5 film (Eastman Kodak Co.) at -70°C. Quantitation of message levels for Cx32 and Cx26 in mouse and rat liver used similar autoradiogmms to those in Fig. 7 (see Nicholson and Zhang, 1988). Equivalent levels of total RNA, as determined by A260were loaded on the original glyoxa ...
Supporting Information for Angew. Chem. Int. Ed. Z52444 © Wiley
Supporting Information for Angew. Chem. Int. Ed. Z52444 © Wiley

... A, C, F, and J) were purchased from the Sigma-Aldrich Chemical Company, Milwaukee, WI. Non-commercially available enantiopure alcohols prepared by palladium-catalyzed oxidative kinetic resolution (Figure 1 entries E,[2] I,[3] and G1,4]) were compared by optical rotation to known values. The previous ...
Investigating The Metal Binding Sites In Znta, A Zinc Transporting
Investigating The Metal Binding Sites In Znta, A Zinc Transporting

... Dr.Kavitha Chendil Kumar, Dr.Pankhuree Verma, Ms.Shilpa Ramanujam and Mr.Dinesh ...
Background for the Recombinant DNA Lab
Background for the Recombinant DNA Lab

... Amplification of the SNP Region Using PCR Next, you will set up a PCR reaction designed to amplify the region of DNA surrounding the SNP (Fig. 1). We need to use PCR so we will have many copies of the particular DNA we are interested in; we do not want to try to experiment with the entire chromosome ...
Solubility and Solubility Equilibrium
Solubility and Solubility Equilibrium

... ions occur when a metal atom is joined by a coordinate covalent bond to neutral molecules and/or ions. For a complex ion, the metal is in the center and the species that bonds to it with coordinate covalent bond are called a ligands. This comes into play regarding solubility because it’s a way of ma ...
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QPNC-PAGE

QPNC-PAGE, or quantitative preparative native continuous polyacrylamide gel electrophoresis, is a high-resolution technique applied in biochemistry and bioinorganic chemistry to separate proteins by isoelectric point. This standardized variant of native gel electrophoresis is used by biologists to isolate active or native metalloproteins in biological samples and to resolve properly and improperly folded metal cofactor-containing proteins or protein isoforms in complex protein mixtures.
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