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04Johnson
04Johnson

... • Carbohydrates are monomers that make up the structural framework of cells and play a critical role in energy storage  a carbohydrate is any molecule that contains the elements C, H, and O in a 1:2:1 ratio  the sizes of carbohydrates varies • simple carbohydrates – made up of one or two monomers ...
Product: Cat. No.: Lot No.: Synonyms: Size: Storage: Usage: Product
Product: Cat. No.: Lot No.: Synonyms: Size: Storage: Usage: Product

... growth factor (HGF), platelet derived growth factor (PDGF), and c-Kit receptors. Also known as Ruk (regulator of ubiquitous kinase) and SETA (SH3 domain-containing gene expressed in tumorigenic astrocytes), CIN85 is an ubiquitously expressed adaptor protein with three SH3 domains. It interacts with ...
Protein functions part 2 File
Protein functions part 2 File

... various ways to form the secondary structure of the protein  Two main types of secondary structure are found in proteins - the beta pleated sheet and the alpha helix  The alpha helix forms when the linear chain coils into a right handed helix  The beta pleated sheet forms when the linear chain fo ...
Proteins - Downtown Magnets High School
Proteins - Downtown Magnets High School

... interact with one another and crystallize into a fiber; capacity to carry oxygen is greatly reduced. ...
CH03_Lecture
CH03_Lecture

... • Once thought newly made proteins folded spontaneously • Chaperone proteins help protein fold correctly • Deficiencies in chaperone proteins implicated in certain diseases – Cystic fibrosis is a hereditary disorder • In some individuals, protein appears to have correct amino acid sequence but fails ...
Cell Membrane Structure & Function
Cell Membrane Structure & Function

... – 1.Membrane selects what substances will enter – 2.Take up molecules present in high concentration – 3 Part of protein extends through bilayer – 4.May be non polar helix beta-pleated sheets of non polar amino acids – 5.Non polar portion held within interior of bilayer – 6.Polar ends protrude from b ...
Lecture8
Lecture8

... • Strategy: plasmid encoded protein that is crucial for survival in the cell culture • Usually antibiotics or essential metabolite has to be added (expensive!) • Risk of gene transfer (e.g., MRSA) • Solution: Integration of the DNA on chromosome ...
Molecules of Life! - Highline Public Schools
Molecules of Life! - Highline Public Schools

... • They are formed by many amino acids linked together • Many polypeptides together make proteins • Proteins are long chains, and are made up of many polypeptides because they have so many different functions in the body ...
Bioanalytical chemistry 8. Gel electrophoresis and blotting
Bioanalytical chemistry 8. Gel electrophoresis and blotting

... (A) a polyacrylamide gel is used to fractionate single-stranded DNA. In the size range 50 to 200 nucleotides, DNA molecules that differ in size by only a single nucleotide can be separated from each other. (B) an agarose gel with medium-sized pores is used to separate double-stranded DNA molecules. ...
Introduction: Proteins are one of the three major classes of biological
Introduction: Proteins are one of the three major classes of biological

... Introduction: Proteins are one of the three major classes of biological macromolecules that perform a variety of functions in a cell. Among other things, they catalyze chemical reactions that drive biological processes. They also provide the physical support for life and pass signals that tell cells ...
Mass spectrometry - Justin Benesch
Mass spectrometry - Justin Benesch

... • The first analyser is used to select an ion population (precursor/parent ions) • The second analyser separates dissociation products (product/daughter ions) • The spectra obtained are often referred to as MS/MS or MS2 spectra • Many different instrument geometries to achieve this: e.g. Q-ToF, ToF-T ...
Chapter5 The Structure and Functionof Macromolecules Discussion
Chapter5 The Structure and Functionof Macromolecules Discussion

... acid. Explain how amino acids may be grouped according to the physical and chemical properties of the R group. 15. Explain what determines protein conformation and why it is important. 16. Explain how the primary structure of a protein is determined. ...
Intro to Biotechnology Chapter 6 Key Points: 6.1: Sources of
Intro to Biotechnology Chapter 6 Key Points: 6.1: Sources of

... of diabetes. c. Use of the foxglove plant to make digitalis, a drug used regulate an irregular heart rate. Some products are produced in such small amount that it is impractical to harness them from nature. Ex. t-PA (tissue plasminogen activator) –dissolves blood clots. It is now given to patients a ...
Slide 1
Slide 1

... disease-causing form (PrpSc). The two structures exhibit two different, classic protein motifs, called "alpha helices," and "beta sheets." Alpha helices, seen here in the normal prion (left), consist of linked aminoacid building blocks that spiral around like a coiled spring. Beta sheets form when a ...
protein - CSU, Chico
protein - CSU, Chico

... Fruitarian: ...
Proteomics - OpenWetWare
Proteomics - OpenWetWare

... Step 1: Isolate cell or other protein source Step 2: Lyse cells and isolate proteins Step 3: Break up proteins into smaller (but still ...
Estimating the Recovery Kinetics of tER Sites
Estimating the Recovery Kinetics of tER Sites

... The transitional Endoplasmic Reticulum (tER) site is a small spot on the Endoplasmic Reticulum that proteins must pass through on their way from the ER to the Golgi apparatus. Through fluorescence recovery after photobleaching (FRAP) imaging techniques the rate proteins attached to specific genes tr ...
No Slide Title
No Slide Title

... Coiled-coil, three separate polypeptides called  chains are supertwisted Provide strength (stronger than ??) Connective tissue (tendons, cartilage, organic matrix of bone, cornea) ...
Chlamydomonas reinhardtii strains carrying the stb1-1
Chlamydomonas reinhardtii strains carrying the stb1-1

... paromomycin resistant clones were screened by zymogram (a). Three complementated strains were obtained from 23 resistant clones for I97 and 4 complementated strains from 15 resistant clones for I73. The gel in (a) show the presence of PhoB in the complementated strain I97C1 (lane 2) and the lack of ...
What are proteins?
What are proteins?

... More ubiquitin added create a multiubiquitin protease complex (Proteasome) • Ubiquitin is ultimately released to be reused in another cycle. ...
Presentazione di PowerPoint
Presentazione di PowerPoint

... • rapeseed and linseed should be tested to evaluate the real potentials and the influence of some specific factors: polyphenolic compounds, amino acid composition, specific constituent (e.g. mucilage, starch..) • the “melt” rheological behavior of oilseed protein is not controlled (cross-linking, ki ...
2.22 Protein Synthesis.docx
2.22 Protein Synthesis.docx

... polypeptide. As shown below, this is a fairly involved process. DNA contains the genetic code that is used as a template to create mRNA in a process known as transcription. The mRNA then moves out of the nucleus into the cytoplasm where it serves as the template for translation, where tRNAs bring in ...
Learning Objectives handouts
Learning Objectives handouts

... 16. Explain what determines protein structure and why it is important. 17. Explain how the primary structure of a protein is determined. 18. Name two types of secondary protein structure. Explain the role of hydrogen bonds in maintaining secondary structure. 19. Explain how weak interactions and dis ...
Simian immunodeficiency virus (SIV) (isolate 216.94.A2) gp120
Simian immunodeficiency virus (SIV) (isolate 216.94.A2) gp120

... Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA. Please contact us for any concerns or special requirements. ...
Blank Jeopardy
Blank Jeopardy

... Maltose, Isomaltose) ...
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QPNC-PAGE

QPNC-PAGE, or quantitative preparative native continuous polyacrylamide gel electrophoresis, is a high-resolution technique applied in biochemistry and bioinorganic chemistry to separate proteins by isoelectric point. This standardized variant of native gel electrophoresis is used by biologists to isolate active or native metalloproteins in biological samples and to resolve properly and improperly folded metal cofactor-containing proteins or protein isoforms in complex protein mixtures.
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