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Biology Unit 2 Organic Notes The Chemistry of Carbon Organic
Biology Unit 2 Organic Notes The Chemistry of Carbon Organic

... Organic chemistry is the study of all compounds that contain bonds between carbon atoms. Macromolecules Macromolecules are formed by a process known as polymerization. Monomers ...
Lh6Ch04aProt
Lh6Ch04aProt

... Structural hierarchy in proteins Structure and function of fibrous proteins Structure analysis of globular proteins Protein folding and denaturation ...
How Do Molecules Cross the Plasma Membrane? 1. Indicate the
How Do Molecules Cross the Plasma Membrane? 1. Indicate the

... 1. Indicate the types of molecules that can diffuse through the lipid bilayer of the plasma membrane, then explain why this can occur. ...
Ion exchange chromatography File
Ion exchange chromatography File

... Resins with low degree of cross-linking are more permeable to high molecular weight compounds, but they are less rigid and swell more when placed in buffer Sulphonation of cross-linked polystyrene results in sulphonated polystyrene resin such as Dowex 50- strong acidic exchanger Basic exchangers are ...
Jalview Homework
Jalview Homework

... “ClustalW Multiple Alignment”. You must be connected to the internet in order for the data to be sent to the server that will perform the multiple alignment. 7. A window telling you the status of the job will appear and then a window will appear displaying the results. Scroll through the alignment a ...
Concept review: Chromatography (applied to protein purification)
Concept review: Chromatography (applied to protein purification)

... • 1. Cell disruption should be performed at cold temperatures. Keep the sample on ice as much as possible and use chilled solutions. This will decrease the activity of the proteases for the simple reasons that all chemical reactions occur more slowly at low temperature. • 2. Add protease inhibitors ...
Bio301 Biochemistry I
Bio301 Biochemistry I

... At high ionic strengths the solubilities ofproteins as well as those of most other substances, decrease. This effect is known as salting out. You have given 1.0 M solutions of NaCl, (NH4) 2SO4 and K3PO4.In which of these solutions would you expect that a protein be most soluble? Least soluble? ...
Understanding the Significance of Proteins, Lipids
Understanding the Significance of Proteins, Lipids

... (carbohydrate) in a creative way. Students should not draw the model on poster board only. They must at least construct one out of the three molecules in a unique way while also comparing it with the two other molecules. For example, if a group of 2 students construct a protein or amino acid, they m ...
DNA and Proteins
DNA and Proteins

... 8. tRNA then goes and finds the corresponding Amino Acid and brings them to the ribosome to be synthesized into protein. 9. The process of converting codons into anticodons and then amino acids is called Translation. More specifically, the mRNA creates codes for the proteins from DNA. These codes oc ...
Biochemistry Webquest
Biochemistry Webquest

... A _________ chain contains hundreds or thousands of _________ linked by _________ bonds – The amino acid _________ causes the _________ to assume a particular _________ If for some reason a protein’s shape is altered, it can no longer function – _________ will cause _________ chains to unravel and l ...
Stress response of some lactic acid bacteria with
Stress response of some lactic acid bacteria with

... S-layer proteins (two strains, Stefan et al., accepted for publication). LAB strains were subjected to different stress conditions: lowest/highest incubation temperatures at which LAB were able to grow (ranging from 15 to 42°C), and addition to the growth medium of NaCl up to 7% (w/v). Growth was fo ...
Biochemistry
Biochemistry

... If the train is the whole polymer, what would be the small groups that make up the train? If the necklace is the polymer, what are the monomers that make up the necklace? ...
The antibody validation experiments were essentially performed as
The antibody validation experiments were essentially performed as

... 7. The growth medium was removed and 40 µl lysis buffer (150 mM NaCl, 50 mM Tris pH 8, 1% Triton X-100, 0.5% Sodium deoxycholate, 0.1% SDS) was added to each well. The plate was then incubated for 30 min on ice. During the incubation the plate was vortexed repeatedly. ...
Macromolecules II
Macromolecules II

... Energy Gained From Protein Eating 1 gram of protein provides your body with 4 Calories. ...
Isaac C. Sanchez
Isaac C. Sanchez

... Computer/Analytical models for the Cold Denaturation of Proteins Computer simulation of cell aggregation & growth onto a tissue ...
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... LIPIDS- Store Energy • Lipids are formed from one glycerol molecule and 3 fatty acids • 3 fatty acids + glycerol ...
Protein Modeling
Protein Modeling

... The Protein Data Bank (PDB) is a repository for the 3-D structural data of large biological molecules, such as proteins and nucleic acids. Obtained by X-ray crystallography or NMR spectroscopy. Submitted by biologists and biochemists from around the world. ...
Lecture_11_2005
Lecture_11_2005

... • Goal: to be able to determine or predict the structure of every protein. – Direct determination - X-ray crystallography and nuclear magentic resonance (NMR). – Prediction • Comparative modeling • Threading/Fold recognition • Ab initio ...
PURExpress® Δ Ribosome Kit
PURExpress® Δ Ribosome Kit

... PURExpress® is based on the PURE System Technology originally developed by Dr. Takuya Ueda at the University of Tokyo and commercialized as the PURESYSTEM® by BioComber (Tokyo, Japan). Licensed from BioComber (Tokyo, Japan) under Patent Nos. 7,118,883; WO2005-105994 and JP2006-340694. For research u ...
Enzymes
Enzymes

... o Involves making and breaking covalent bonds Predicating Chemical Reactions - Free energy (G) o Energy that can be extracted from a system to do work or drive a chemical reaction when temperature is uniform (as in a cell) (expressed as kcal/mole) o measure of a systems instability or tendency to ch ...
Molecules of Life
Molecules of Life

... energy. Fats can be saturated, which means that they are a solid at room temperature like lard, or unsaturated, which are liquids at room temperature like vegetable oil. All lipids are hydrophobic. This means that they do not mix with water. If you make salad dressing using oil and water, you can sh ...
Ch. 2 The Chemistry of Life
Ch. 2 The Chemistry of Life

... - pH scale - _________________ system to indicate the _______________ of ____ ions in ______________, ranges from _______ - Acid – any ______________ that forms _____ ions in ____________ - __________ solutions have __________ concentration of ____ ions than pure _________ & have ____ values _______ ...
Lecture_10
Lecture_10

... Christian Anfinsen placed the enzyme ribonuclease, which degrades RNA, in a solution containing urea or guanidinium chloride and β-mercaptoethanol. Urea or guanidinium chloride destroyed all noncovalent bonds, while the β-mercaptoethanol destroyed the disulfide bonds. The enzyme displayed no enzy ...
Note 7.4 - Controlling Gene Expression
Note 7.4 - Controlling Gene Expression

... the availability to function. There are three methods for controlling the functionality of a protein. The initial protein produced is in an inactive form, controlled by processing mechanisms. (Insulin is produced in an inactive form called proinsulin) Processing mechanisms can remove specific sectio ...
MolBioIntro
MolBioIntro

... The ability to respire, turning nutrients into energy. For life on Earth, this process is glycolysis, and the storage of energy is maintained by ATP. Sensitivity to the outside world, and an ability to react. Lions maul you if you poke them with a stick, plants will grow their roots out, E. coli bac ...
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QPNC-PAGE

QPNC-PAGE, or quantitative preparative native continuous polyacrylamide gel electrophoresis, is a high-resolution technique applied in biochemistry and bioinorganic chemistry to separate proteins by isoelectric point. This standardized variant of native gel electrophoresis is used by biologists to isolate active or native metalloproteins in biological samples and to resolve properly and improperly folded metal cofactor-containing proteins or protein isoforms in complex protein mixtures.
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