Globular proteins
... producing a pattern of spots with various intensities. By analysis of the x-ray diffraction pattern, spacing between different atoms of the molecule can be determined. X-ray diffraction pattern can give an idea about the electron densities in three dimensional space. Many proteins have been crystall ...
... producing a pattern of spots with various intensities. By analysis of the x-ray diffraction pattern, spacing between different atoms of the molecule can be determined. X-ray diffraction pattern can give an idea about the electron densities in three dimensional space. Many proteins have been crystall ...
CH 6: Proteins and Amino Acids
... Amino Acids • Diet must provide all 9 of the essential a.a. on a regular/daily basis for proteins to be made – Need all 20 a.a. to make most proteins – Animal sources of proteins contain all 9 essential ...
... Amino Acids • Diet must provide all 9 of the essential a.a. on a regular/daily basis for proteins to be made – Need all 20 a.a. to make most proteins – Animal sources of proteins contain all 9 essential ...
Improved recovery of DNA from polyacrylamide gels after in situ
... nondenaturing acrylamide gels following band shift assays (Papavassiliou, 1994). If the complexes are sufficiently stable it is also possible to do the reverse: apply footprinted DNA(-complexes) to a nondenaturing acrylamide gel to separate the protein – DNA complexes. The critical step common to bo ...
... nondenaturing acrylamide gels following band shift assays (Papavassiliou, 1994). If the complexes are sufficiently stable it is also possible to do the reverse: apply footprinted DNA(-complexes) to a nondenaturing acrylamide gel to separate the protein – DNA complexes. The critical step common to bo ...
B-PERfusions
... alternative to sonication for extracting recombinant proteins from the cytoplasm of Escherichia coli cells carrying expression vectors, especially when many proteins are to be processed in parallel. In this article I’ll summarize our extensive experience with B-PER for isolating fusion proteins in w ...
... alternative to sonication for extracting recombinant proteins from the cytoplasm of Escherichia coli cells carrying expression vectors, especially when many proteins are to be processed in parallel. In this article I’ll summarize our extensive experience with B-PER for isolating fusion proteins in w ...
PROTEIN STRUCTURE SIMILARITY CALCULATION AND VISUALIZATION
... Display/write the pairwise similarity between each protein file as lower triangular matrix for comparison purpose ...
... Display/write the pairwise similarity between each protein file as lower triangular matrix for comparison purpose ...
How to Select for Enzymes
... - General scheme for selection of enzymes from protein libraries > 1012 - Product formation as only selection criterion - Novel RNA-ligases from Zinc-finger library ...
... - General scheme for selection of enzymes from protein libraries > 1012 - Product formation as only selection criterion - Novel RNA-ligases from Zinc-finger library ...
say “cheese!”
... protein is caused by the specific amino acids that are linked together in the chain. These amino acids react with each other and cause the protein chain to twist and fold up into a large 3-D shape, forming a globular protein. The “R” groups (or side groups) of each amino acid can be either hydrophob ...
... protein is caused by the specific amino acids that are linked together in the chain. These amino acids react with each other and cause the protein chain to twist and fold up into a large 3-D shape, forming a globular protein. The “R” groups (or side groups) of each amino acid can be either hydrophob ...
Methods for Control of Microbial Growth
... • Incineration/ baking achieve sterility • Autoclaving: sterilization with live steam and pressure, very widely used • Pasteurization: flash heat treatment (63°C - 66°C for 30 minutes) that reduces the bio-burden of food materials (kills Salmonella and Listeria) • Boiling disinfection – does not ach ...
... • Incineration/ baking achieve sterility • Autoclaving: sterilization with live steam and pressure, very widely used • Pasteurization: flash heat treatment (63°C - 66°C for 30 minutes) that reduces the bio-burden of food materials (kills Salmonella and Listeria) • Boiling disinfection – does not ach ...
Bio A
... Carbohydrates are molecules that provide quick energy for the cell, and can be used for structure in certain organisms Carbohydrates are made up of carbon, hydrogen and oxygen which are shaped in a ring ...
... Carbohydrates are molecules that provide quick energy for the cell, and can be used for structure in certain organisms Carbohydrates are made up of carbon, hydrogen and oxygen which are shaped in a ring ...
Chapter 34-4B: Second Messengers
... Some of membrane proteins are receptors of signal transduction. Hormones, growth factors and neurotransmitters bind the signal transduction receptors located on the surface of target cell membranes. Signals are transferred to the inside of target cell through the transmembrane receptor protein. Sign ...
... Some of membrane proteins are receptors of signal transduction. Hormones, growth factors and neurotransmitters bind the signal transduction receptors located on the surface of target cell membranes. Signals are transferred to the inside of target cell through the transmembrane receptor protein. Sign ...
Prof. Dr. Harry F. Noller Prof. Dr. Ada Yonath
... synthesis is done in the ribosomes, a large number of which act simultaneously on the same mRNA synthesizing different proteins. These ribosomes, which are the focus of the Prize today, are giant cellular assemblies, each composed of two subunits comprising mainly RNA, in which more than 50 proteins ...
... synthesis is done in the ribosomes, a large number of which act simultaneously on the same mRNA synthesizing different proteins. These ribosomes, which are the focus of the Prize today, are giant cellular assemblies, each composed of two subunits comprising mainly RNA, in which more than 50 proteins ...
lab9
... negatively charged DNA pieces will move and migrate to the positive (+) pole depending on their molecular weight. ...
... negatively charged DNA pieces will move and migrate to the positive (+) pole depending on their molecular weight. ...
Chemical Level of Organization
... • Secondary structure: alpha helix or pleated sheet created by H-bonds • Tertiary structure: folded 3-D shape due to H, ionic, & covalent bonds, polar and non-polar attractions • Quaternary structure: attractions between separate protein molecules Proteins - Activity • Activity affected by 3-D shape ...
... • Secondary structure: alpha helix or pleated sheet created by H-bonds • Tertiary structure: folded 3-D shape due to H, ionic, & covalent bonds, polar and non-polar attractions • Quaternary structure: attractions between separate protein molecules Proteins - Activity • Activity affected by 3-D shape ...
Ms. Robyn Klemptner
... Antibody-based affinity capture Chemical derivatization Metal ion-based affinity capture Thus, more sensitive and reliable method required = DENDRIMERS! Novel proteome investigation in plants since dendrimerbased enrichment techniques have yet to be applied to plant studies. (Meimoun et al., 2 ...
... Antibody-based affinity capture Chemical derivatization Metal ion-based affinity capture Thus, more sensitive and reliable method required = DENDRIMERS! Novel proteome investigation in plants since dendrimerbased enrichment techniques have yet to be applied to plant studies. (Meimoun et al., 2 ...
Representation and Manipulation of 3D Molecular Structures
... • Data mining for conformational properties and intermolecular interactions (CSD & PDB) • Data mining for information about intermolecular interactions (CSD & PDB) • Further understanding of the nature of protein structure and its relationship to amino acid sequence (PDB) • Homology modeling (compar ...
... • Data mining for conformational properties and intermolecular interactions (CSD & PDB) • Data mining for information about intermolecular interactions (CSD & PDB) • Further understanding of the nature of protein structure and its relationship to amino acid sequence (PDB) • Homology modeling (compar ...
Field Analyzer
... Pulsed field gel electrophoresis is a collection of techniques to increase the resolution of DNA fragments to extend to very large DNA fragments (>>100 kbp) based on the size-dependent relaxation properties of DNA DNA elongates along the direction of the electric field (E) DNA relaxes when the field ...
... Pulsed field gel electrophoresis is a collection of techniques to increase the resolution of DNA fragments to extend to very large DNA fragments (>>100 kbp) based on the size-dependent relaxation properties of DNA DNA elongates along the direction of the electric field (E) DNA relaxes when the field ...
Protein-Misfolding Diseases
... General facts on protein folding Causes of misfolding and protein aggregation Cellular consequences of protein aggregation Protein-misfolding diseases Amyloidoses Amyloid fibril formation Neurodegenerative diseases and Alzheimer’s disease Therapy solutions Curing Alzheimer’s disease ...
... General facts on protein folding Causes of misfolding and protein aggregation Cellular consequences of protein aggregation Protein-misfolding diseases Amyloidoses Amyloid fibril formation Neurodegenerative diseases and Alzheimer’s disease Therapy solutions Curing Alzheimer’s disease ...
Binding Kinetics of Protein Lipid Interactions Using OpenSPR
... measurements were obtained using OpenSPR. Tests were run at 20 µL/min using HBS running buffer. LIP-1 Sensors were first cleaned with 40 mM Octyl βD-glucopyranoside and 20 mM CHAPS. 3. Liposomes (0.17 µg/mL) were run over the chip surface until 1 nm of immobilization was observed. An injection of 1% ...
... measurements were obtained using OpenSPR. Tests were run at 20 µL/min using HBS running buffer. LIP-1 Sensors were first cleaned with 40 mM Octyl βD-glucopyranoside and 20 mM CHAPS. 3. Liposomes (0.17 µg/mL) were run over the chip surface until 1 nm of immobilization was observed. An injection of 1% ...
Lecture
... Function: Support Examples: Keratin is the protein of hair, horns, feathers, and other skin appendages. Insects and spiders use silk fibers to make their cocoons and webs, respectively. Collagen and elastin proteins provide a fibrous framework in animal connective tissues. ...
... Function: Support Examples: Keratin is the protein of hair, horns, feathers, and other skin appendages. Insects and spiders use silk fibers to make their cocoons and webs, respectively. Collagen and elastin proteins provide a fibrous framework in animal connective tissues. ...
AMINO ACIDS IN PROTEINS
... Biologically active proteins Complex tertiary structure often with several types of secondary interactions within the same polypeptide chain ...
... Biologically active proteins Complex tertiary structure often with several types of secondary interactions within the same polypeptide chain ...
Poster - Protein Information Resource
... The PIRSF protein classification system reflects evolutionary relationship of full-length proteins and domains. PIRSF families are extensively curated using a bioinformatics infrastructure implemented in a J2EE framework. Expert manual curation includes membership, annotation of specific biological ...
... The PIRSF protein classification system reflects evolutionary relationship of full-length proteins and domains. PIRSF families are extensively curated using a bioinformatics infrastructure implemented in a J2EE framework. Expert manual curation includes membership, annotation of specific biological ...
Capillary Electrophoresis
... Separating trace amounts of ionic species can be a highly difficult and tedious process. The separation process depends on the ions having different migration speeds. The velocity of ion migration [m/s], is defined by:: ...
... Separating trace amounts of ionic species can be a highly difficult and tedious process. The separation process depends on the ions having different migration speeds. The velocity of ion migration [m/s], is defined by:: ...
Proteins
... The subunit of a more complex protein has now been formed. It may be globular or fibrous. It now has its functional shape or conformation. ...
... The subunit of a more complex protein has now been formed. It may be globular or fibrous. It now has its functional shape or conformation. ...