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gln.val.tyr.ala lys.arg.glu.trp met.his.leu.asp cys.pro.gly.asn F-A-D
gln.val.tyr.ala lys.arg.glu.trp met.his.leu.asp cys.pro.gly.asn F-A-D

... You have isolated an octapeptide with the amino acid composition (Lys2, Asp, Tyr, Phe, Gly, Ser, Ala) Reaction of the intact peptide with FDNB yields DNP-alanine. Cleavage with trypsin yields peptides with compositions (Lys, Ala, Ser) and (Gly, Phe, Lys) plus a dipeptide. Reaction with chymotrypsin ...
Molecules of Life Powerpoint
Molecules of Life Powerpoint

... • A fourth class of lipids is the waxes, each of which is composed of a single fatty acid linked to a long-chain alcohol. • Waxes have an important “sealing” function in the living world. • Almost all plant surfaces exposed to air, for example, have a protective covering made largely of wax. ...
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... • Lipids are the one class of large biological molecules that do not form polymers • The unifying feature of lipids is having little or no affinity for water • Lipids are hydrophobic because they consist mostly of hydrocarbons, which form nonpolar covalent bonds ...
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...  What determines Protein Conformation? The sequence of the polypeptide  Denaturaton The loss of protein conformation  What causes the denaturation? Physical & Environmental alteration such as pH, temperature, salt concentration ...
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... a. What enzyme complex is involved in this disease and what are its components? What reaction does this complex catalyze? Branched chain keto acid dehydrogenase. Three components can be affected: E1-keto acid decarboxylase (thiamine dependent) either alpha (IA) or beta (IB); E2-dihydrolipoyl acyltr ...
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... favorable form that it will assume. However, for other proteins, a particular sequence may have several forms that are just as energetically favorable as another. These proteins require a helper molecule, known as a chaperone, to help it fold correctly. ...
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... Cholesterol: An essential lipid molecule needed for cell membranes. Lipids act as a barrier that contains the liquid parts of the cell. They also act as chemical messengers in the form of hormones. ...
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... Genetic code is univeral (almost) • All organisms so far examined use the code as originally deduced (or something very close to it). • The rare exceptions involve limited differences. – e.g. In RNA derived from mitochondrial DNA, UGA encodes Trp instead of serving as a stop codon. – Thus UGA and U ...
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... their cells are making new proteins and other nitrogen compounds. Several conditions result in a negative nitrogen balance-the excretion of more nitrogen than is consumed.During starvation and certain diseases, the carbon skeleton of amino acids derived from the breakdor,rmof muscle proteins must be ...
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... Proteins show 4 levels of structural organisation: 1. Primary structure = amino acid sequence • Determined by the genetic code of the mRNA. 2. Secondary structure = folding and twisting of a single polypeptide chain. • Result of weak H-bond and electrostatic interactions. • e.g., -helix (coiled) an ...
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... bonds; they are classified by the number of amino acids in the chain. ◦ Dipeptide: A molecule containing two amino acids joined by a peptide bond. ◦ Tripeptide: A molecule containing three amino acids joined by peptide bonds. ◦ Polypeptide: A macromolecule containing many amino acids joined by pepti ...
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24.9 Synthesis of Amino Acids
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... 24.9 Synthesis of Amino Acids Plants and bacteria produce all of their amino acids using NH4+ and NO3–. • Humans can synthesize 9 of the 20 amino acids found in their proteins. • Nonessential amino acids are synthesized in the body, while essential amino acids must be obtained from diet. ...
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... Major interfering agents: Detergents, nucleic acids, particulates, lipid droplets Highly susceptible to contamination by buffers, biological materials and salts Protein amino acid composition is extremely important, thus the choice of a standard is very difficult, especially for purified proteins Ab ...
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Biosynthesis



Biosynthesis (also called biogenesis or anabolism) is a multi-step, enzyme-catalyzed process where substrates are converted into more complex products in living organisms. In biosynthesis, simple compounds are modified, converted into other compounds, or joined together to form macromolecules. This process often consists of metabolic pathways. Some of these biosynthetic pathways are located within a single cellular organelle, while others involve enzymes that are located within multiple cellular organelles. Examples of these biosynthetic pathways include the production of lipid membrane components and nucleotides.The prerequisite elements for biosynthesis include: precursor compounds, chemical energy (e.g. ATP), and catalytic enzymes which may require coenzymes (e.g.NADH, NADPH). These elements create monomers, the building blocks for macromolecules. Some important biological macromolecules include: proteins, which are composed of amino acid monomers joined via peptide bonds, and DNA molecules, which are composed of nucleotides joined via phosphodiester bonds.
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