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Stable Isotope Labeling with Amino Acids in Cell Culture (SILAC)
Stable Isotope Labeling with Amino Acids in Cell Culture (SILAC)

... C labeled L-lysine, respectively). When the labeled analog of an amino acid is supplied to cells in culture instead of the natural amino acid, it is incorporated into all newly synthesized proteins. After a number of cell divisions, each instance of this particular amino acid will be replaced by its ...
Biochem Midterm - Website of Neelay Gandhi
Biochem Midterm - Website of Neelay Gandhi

HonBio Chapter 3 notes
HonBio Chapter 3 notes

... Nucleic Acids ...
Chap 3 - CRCBiologyY11
Chap 3 - CRCBiologyY11

... • There are two kinds of nucleic acids: 1. Deoxyribonucleic acid (DNA) – located in chromosomes in the nucleus of eukaryotic cells. Each nucleotide unit has: - a sugar (deoxyribose) part, - a phosphate part and - a N-containing base. The four different N-containing bases are adenine (A), thymine (T) ...
Differences between DNA and RNA • Ribonucleic acid is similar to
Differences between DNA and RNA • Ribonucleic acid is similar to

... DNA consists of 2 complementary strands, whereas RNA generally exists as a single strand. ...
CH 16 PPT
CH 16 PPT

... Helicase:catalyzes the untwisting of the DNA at the replication fork DNA polymerase:catalyzes the elongation of new DNA ...
Chapter 2
Chapter 2

... RNA plays an important role in the production of the particular protein which a cell needs. In the following, we shall endeavor to give a general outline as to how the genetic code in DNA finally produces the protein. The first part of the protein synthesis process is called transcription consisting ...
Structure and Function of DNA
Structure and Function of DNA

... opposite strand by base pairing with free-floating nucleotides. Each guanine (G) pairs with cytosine (C), while each thymine (T) pairs with an adenine (A). ...
Biochemistry
Biochemistry

... •Fats and oils – store long term energy Found in animal fats, vegetable oil, petroleum. ...
what is your dna alias
what is your dna alias

... bases together in a sequence on a DNA strand are called a ‘codon’. Because there are so many possible base sequences (i.e., codons), geneticists have developed a short-hand using our 26 letter alphabet. Remember that 3 bases together define a specific amino acid. And two or more amino acids make a p ...
Peptides and Proteins
Peptides and Proteins

... 20 amino acids are commonly found in protein. These 20 amino acids are linked together through “peptide bond forming peptides and proteins. - The chains containing less than 50 amino acids are called “peptides”, while those containing greater than 50 amino acids are called “proteins”. Peptide bond f ...
3-3-16 Biology Bell Work: Where does DNA replication take place
3-3-16 Biology Bell Work: Where does DNA replication take place

Amino Acids 20 – Bloodspot
Amino Acids 20 – Bloodspot

Sec_2_3 Carbon Compunds
Sec_2_3 Carbon Compunds

SLG MOCK MIDTERM – FOR PRACTICE ONLY
SLG MOCK MIDTERM – FOR PRACTICE ONLY

... contain errors, intentional or otherwise. It is up to the student to verify the information contained within. ...
Biochemical Thermodynamics
Biochemical Thermodynamics

...  ATP + Ser-OH  ADP + Ser-O-(P)  Often involved in activating or inactivating enzymes  Under careful control of enzymes called kinases and phosphatases ...
CH 5
CH 5

Chemistry of Life
Chemistry of Life

... • Stores energy from break down of glucose – Transfers phosphate groups to release energy = ...
Create the complementary strand for the following
Create the complementary strand for the following

... make proteins? -DNA is used as directions for making proteins.  -DNA cannot leave the nucleus, so in order to make proteins, RNA must be made.  -RNA is much like DNA however it only has one strand and instead of using the nucleotide Thymine (T) it uses the nucleotide Uracil (U). (A=U)  -RNA uses ...
investigating dna
investigating dna

... Every gene code has a unique and specific protein. Each protein has a specialized role within the cell or used elsewhere in the organism. It is important to understand that DNA does not directly code for proteins such as blood type or how much insulin to produce. Instead, there is two step process c ...
Name: Pd: _____ Date: Modeling Protein Structure Background
Name: Pd: _____ Date: Modeling Protein Structure Background

... by the DNA code. A chain of amino acids is called a polypeptide chain and is considered the primary structure of a protein. The amino and carboxyl groups of the amino acids along the chain will interact forming the secondary structure. The secondary structure is usually an alpha helix or beta-pleate ...
Slide 1
Slide 1

... Free RNA nucleotides ...
CSM 101 Fall 2010 Timeline
CSM 101 Fall 2010 Timeline

... Each colored section of the structure represents one tertiary structure, so in the picture there are four tertiary structures ...
Zoo/Bot 3333
Zoo/Bot 3333

... blot analysis. The probe used in this instance hybridizes to a DNA fragment linked to the disease gene, which shows polymorphism for this restriction enzyme. The autoradiogram of this blot is shown above, aligned with the family pedigree. 5. In the above example, which of the following are likely t ...
Lesson on Proteins
Lesson on Proteins

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Biosynthesis



Biosynthesis (also called biogenesis or anabolism) is a multi-step, enzyme-catalyzed process where substrates are converted into more complex products in living organisms. In biosynthesis, simple compounds are modified, converted into other compounds, or joined together to form macromolecules. This process often consists of metabolic pathways. Some of these biosynthetic pathways are located within a single cellular organelle, while others involve enzymes that are located within multiple cellular organelles. Examples of these biosynthetic pathways include the production of lipid membrane components and nucleotides.The prerequisite elements for biosynthesis include: precursor compounds, chemical energy (e.g. ATP), and catalytic enzymes which may require coenzymes (e.g.NADH, NADPH). These elements create monomers, the building blocks for macromolecules. Some important biological macromolecules include: proteins, which are composed of amino acid monomers joined via peptide bonds, and DNA molecules, which are composed of nucleotides joined via phosphodiester bonds.
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