Document
... # It is used to purify enveloped viruses & ribosomes. # It will also separate cell organelles from crude cellular extracts. # It will also purify exosomes. ...
... # It is used to purify enveloped viruses & ribosomes. # It will also separate cell organelles from crude cellular extracts. # It will also purify exosomes. ...
Forensic Science
... •The smaller DNA fragments will move at a faster rate on the gel plate than the larger ones. •The fragments are then transferred to a nylon membrane in a process called Southern blotting. •To visualize the RFLPs, the nylon sheet is treated with radioactive probes containing a base sequence complemen ...
... •The smaller DNA fragments will move at a faster rate on the gel plate than the larger ones. •The fragments are then transferred to a nylon membrane in a process called Southern blotting. •To visualize the RFLPs, the nylon sheet is treated with radioactive probes containing a base sequence complemen ...
Tutorial - 2: Anti-cancer agents: Antimetabolites, Antibiotics, Plant
... 3. Write the mechanism of action, uses and side effects of one DNA polymerase/ DNA chain elongation inhibitors as an anticancer agent. 4. The anti-cancer drug 6-mercaptopurine is rapidly metabolized in the liver by xanthene oxidase (XO) enzyme into the inactive metabolite (6-thiouric acid). Briefly ...
... 3. Write the mechanism of action, uses and side effects of one DNA polymerase/ DNA chain elongation inhibitors as an anticancer agent. 4. The anti-cancer drug 6-mercaptopurine is rapidly metabolized in the liver by xanthene oxidase (XO) enzyme into the inactive metabolite (6-thiouric acid). Briefly ...
Chapter 16 The Molecular Basis of Inheritance
... – Supported the semiconservative model of DNA replication EXPERIMENT Matthew Meselson and Franklin Stahl cultured E. coli bacteria for several generations on a medium containing nucleotide precursors labeled with a heavy isotope of nitrogen, 15N. The bacteria incorporated the heavy nitrogen into the ...
... – Supported the semiconservative model of DNA replication EXPERIMENT Matthew Meselson and Franklin Stahl cultured E. coli bacteria for several generations on a medium containing nucleotide precursors labeled with a heavy isotope of nitrogen, 15N. The bacteria incorporated the heavy nitrogen into the ...
Amino acids and insulin act additively to regulate components of the
... Results: Incubation of C2C12 myotubes with 0.2 × physiological amino acids concentration (0.2 × PC AA), relative to 1.0 × PC AA, significantly increased total proteolysis and the expression of 14-kDa E2 ubiquitin conjugating enzyme (p < 0.05). The proteasome inhibitor MG132 blocked the rise in prote ...
... Results: Incubation of C2C12 myotubes with 0.2 × physiological amino acids concentration (0.2 × PC AA), relative to 1.0 × PC AA, significantly increased total proteolysis and the expression of 14-kDa E2 ubiquitin conjugating enzyme (p < 0.05). The proteasome inhibitor MG132 blocked the rise in prote ...
Chapter 8
... – MOTION energy in the movement of objects. The faster they move, the more energy. Wind is motion energy. When a car comes to a total stop, releases all motion energy in uncontrolled instant. – SOUND -movement of energy through substances in longitudinal waves. Sound produced when force causes objec ...
... – MOTION energy in the movement of objects. The faster they move, the more energy. Wind is motion energy. When a car comes to a total stop, releases all motion energy in uncontrolled instant. – SOUND -movement of energy through substances in longitudinal waves. Sound produced when force causes objec ...
Dinazyme C/S
... Reactions are carried out by numerous enzymes with different roles. Industrial enzymes originate from biological systems which contribute to sustainable development through being isolated from microorganisms which are fermented using primarily renewable resources. Small amounts of enzymes are requir ...
... Reactions are carried out by numerous enzymes with different roles. Industrial enzymes originate from biological systems which contribute to sustainable development through being isolated from microorganisms which are fermented using primarily renewable resources. Small amounts of enzymes are requir ...
The Structure and Function of Large Biological Molecules
... • Many herbivores, from cows to termites, have symbiotic relationships with these microbes ...
... • Many herbivores, from cows to termites, have symbiotic relationships with these microbes ...
Assessment of the mathematical issues involved
... administering a large quantity of a specific amino acid on protein synthesis is a difficult task. There is a large body of literature, which has recently been reviewed in [4], indicating that leucine flooding doses promote protein synthesis in vitro when other amino acids are plentiful. One should b ...
... administering a large quantity of a specific amino acid on protein synthesis is a difficult task. There is a large body of literature, which has recently been reviewed in [4], indicating that leucine flooding doses promote protein synthesis in vitro when other amino acids are plentiful. One should b ...
Intro to and Thermodynamics In Metabolism:
... No structures. Know the energy production of the “high energy compounds”: ATP, PEP, 1,3-BPG and phosphocreatine. Understand the difference between substrate-level and oxidative phosphorylation. Don’t worry about the diagram showing where all of the Phosphate comes from and goes to. Know the terms th ...
... No structures. Know the energy production of the “high energy compounds”: ATP, PEP, 1,3-BPG and phosphocreatine. Understand the difference between substrate-level and oxidative phosphorylation. Don’t worry about the diagram showing where all of the Phosphate comes from and goes to. Know the terms th ...
Enzymes
... performed to measure enzyme activity. • Assays for blood enzymes are routinely performed in clinical labs. • Assays are often done by monitoring the rate at which a characteristic color of a product forms or the color of a substrate decreases. - For reactions involving H+ ions, the rate of change in ...
... performed to measure enzyme activity. • Assays for blood enzymes are routinely performed in clinical labs. • Assays are often done by monitoring the rate at which a characteristic color of a product forms or the color of a substrate decreases. - For reactions involving H+ ions, the rate of change in ...
Biotech Lect-10 - ASAB-NUST
... Microbial Biotechnology in Food and Agriculture • reduction in the reliance on chemical treatments to control weeds by engineering herbicide tolerance into crops • production of products that have high yield and enhanced nutritional value ...
... Microbial Biotechnology in Food and Agriculture • reduction in the reliance on chemical treatments to control weeds by engineering herbicide tolerance into crops • production of products that have high yield and enhanced nutritional value ...
The citric acid cycle is the
... of any molecule that can be transformed into an acetyl group or dicarboxylic acid. • The cycle is also an important source of precursors, not only for the storage forms of fuels, but also for the building blocks of many other molecules such as amino acids, nucleotide bases, cholesterol, and porphyri ...
... of any molecule that can be transformed into an acetyl group or dicarboxylic acid. • The cycle is also an important source of precursors, not only for the storage forms of fuels, but also for the building blocks of many other molecules such as amino acids, nucleotide bases, cholesterol, and porphyri ...
fuels and tissues
... formation of 2, 3 bis-phosphoglycerate for maintenance of low affinity form of haemoglobin ...
... formation of 2, 3 bis-phosphoglycerate for maintenance of low affinity form of haemoglobin ...
A Novel Activity for Fungal Nitronate Monooxygenase: Detoxification
... Nitronate monooxygenase (NMO; E.C. 1.13.12.16) is an FMN dependent enzyme that oxidizes alkyl nitronates to their corresponding carbonyl compounds and nitrite. While the kinetic and mechanistic properties of NMO from Neurospora crassa and Williopsis saturnus var. Mrakii have been extensively charact ...
... Nitronate monooxygenase (NMO; E.C. 1.13.12.16) is an FMN dependent enzyme that oxidizes alkyl nitronates to their corresponding carbonyl compounds and nitrite. While the kinetic and mechanistic properties of NMO from Neurospora crassa and Williopsis saturnus var. Mrakii have been extensively charact ...
An ACP-Independent Fatty Acid Synthesis Pathway in Archaea
... archaeal sequences into two monophyletic groups, strongly suggesting that the cenancestor was endowed with these two enzymes. In contrast, KAS is widespread in bacteria and absent in archaea. Thiolases and HMGS catalyze the removal of an acetyl-CoA moiety from an acyl-CoA chain or the opposite conde ...
... archaeal sequences into two monophyletic groups, strongly suggesting that the cenancestor was endowed with these two enzymes. In contrast, KAS is widespread in bacteria and absent in archaea. Thiolases and HMGS catalyze the removal of an acetyl-CoA moiety from an acyl-CoA chain or the opposite conde ...
7-cellular-respiration
... many other pathways because they produce the energy needed for them as ATP. ATP = adenosine triphosphate ...
... many other pathways because they produce the energy needed for them as ATP. ATP = adenosine triphosphate ...
Metabolism & Enzymes - T.R. Robinson High School
... 3-D structure of enzyme fits substrate substrate binding cause enzyme to change shape leading to a tighter fit ...
... 3-D structure of enzyme fits substrate substrate binding cause enzyme to change shape leading to a tighter fit ...
Document
... – Some are obtained directly from food – Others are made from intermediates in glycolysis and the Krebs cycle ...
... – Some are obtained directly from food – Others are made from intermediates in glycolysis and the Krebs cycle ...
Biosynthesis
Biosynthesis (also called biogenesis or anabolism) is a multi-step, enzyme-catalyzed process where substrates are converted into more complex products in living organisms. In biosynthesis, simple compounds are modified, converted into other compounds, or joined together to form macromolecules. This process often consists of metabolic pathways. Some of these biosynthetic pathways are located within a single cellular organelle, while others involve enzymes that are located within multiple cellular organelles. Examples of these biosynthetic pathways include the production of lipid membrane components and nucleotides.The prerequisite elements for biosynthesis include: precursor compounds, chemical energy (e.g. ATP), and catalytic enzymes which may require coenzymes (e.g.NADH, NADPH). These elements create monomers, the building blocks for macromolecules. Some important biological macromolecules include: proteins, which are composed of amino acid monomers joined via peptide bonds, and DNA molecules, which are composed of nucleotides joined via phosphodiester bonds.