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Nucleotide sequences of immunoglobulin heavy and light chain V
Nucleotide sequences of immunoglobulin heavy and light chain V

... complexes (1). The epitope recognized by this murine autoantibody is distinct from Sm or RNP epitopes (1—3). F78 is a unique tool for characterization of snRNPs because of the distinct nature of the snRNP complex it recognizes. Immunoprecipitation studies have revealed that this antibody recognizes ...
Application Note #14 - GE Healthcare Life Sciences
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... produced the sequence Val17-Gly-Ser-Asn-Val-, Val being at position 17 of the HM2 chain. Cleavage at position 47 with trypsin followed by RP-HPLC separation of the two resulting peptides yielded a fragment whose sequence was found to match the C-terminal sequence from positions 48 to 64. Finally, ma ...
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Electron Transport Chain (ETC)
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biochemistry module elective course contents
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MECHANISTIC STUDIES ON THE MONOAMINE OXIDASE B

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... Proteins – General: • > 50 AA • Linus Pauling – 1954 Nobel Prize  α-helix and β-pleated sheet • Fredrick Sanger – 1958  Primary structure of beef insulin ...
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Biosynthesis



Biosynthesis (also called biogenesis or anabolism) is a multi-step, enzyme-catalyzed process where substrates are converted into more complex products in living organisms. In biosynthesis, simple compounds are modified, converted into other compounds, or joined together to form macromolecules. This process often consists of metabolic pathways. Some of these biosynthetic pathways are located within a single cellular organelle, while others involve enzymes that are located within multiple cellular organelles. Examples of these biosynthetic pathways include the production of lipid membrane components and nucleotides.The prerequisite elements for biosynthesis include: precursor compounds, chemical energy (e.g. ATP), and catalytic enzymes which may require coenzymes (e.g.NADH, NADPH). These elements create monomers, the building blocks for macromolecules. Some important biological macromolecules include: proteins, which are composed of amino acid monomers joined via peptide bonds, and DNA molecules, which are composed of nucleotides joined via phosphodiester bonds.
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