Site-Directed Mutagenesis of Predicted Active Site Residues in
... of total protein from the cell lysate was assayed in a final volume of 250 ml using 30 nM N-acetyl-L-aspartyl-L-[3,4-3H]glutamate in 50 mM Tris, pH 7.4, at 37°C, 0.1% Triton X-100, 150 mM potassium phosphate, and 1 mM CoCl2 (Robinson et al., 1987). Total protein was assayed using the bicinchoninic a ...
... of total protein from the cell lysate was assayed in a final volume of 250 ml using 30 nM N-acetyl-L-aspartyl-L-[3,4-3H]glutamate in 50 mM Tris, pH 7.4, at 37°C, 0.1% Triton X-100, 150 mM potassium phosphate, and 1 mM CoCl2 (Robinson et al., 1987). Total protein was assayed using the bicinchoninic a ...
Molecular Cloning, Characterization, and mRNA Expression of
... Preparation of fatty acid sodium salt and fatty acid-BSA complex was made according to the method of Alvaro et al. (2010). Briefly, 10 mg of fatty acids (oleic acid, linoleic acid, α-linolenic acid, palmitic acid and arachidonic acid) (Sigma-Aldrich, St. Louis, USA) were mixed with 0.5 ml EtOH and 5 ...
... Preparation of fatty acid sodium salt and fatty acid-BSA complex was made according to the method of Alvaro et al. (2010). Briefly, 10 mg of fatty acids (oleic acid, linoleic acid, α-linolenic acid, palmitic acid and arachidonic acid) (Sigma-Aldrich, St. Louis, USA) were mixed with 0.5 ml EtOH and 5 ...
File
... Hydration of ethaneWater is added to ethane by passing a mixture of steam and ethane over a phosphoric acid in silica gel catalyst at 300°C, 60 atm. ...
... Hydration of ethaneWater is added to ethane by passing a mixture of steam and ethane over a phosphoric acid in silica gel catalyst at 300°C, 60 atm. ...
Cellular Respiration
... • The enzyme lactate dehydrogenase converts pyruvate into lactic acid and converts NADH into NAD+. • Usually blood can remove the lactate, however if this does not happen muscle fatigue results. ...
... • The enzyme lactate dehydrogenase converts pyruvate into lactic acid and converts NADH into NAD+. • Usually blood can remove the lactate, however if this does not happen muscle fatigue results. ...
Analysis of distribution of bases in the coding
... amino acid frequencies for different species is small, too. In other words, the distributions of frequencies of amino acids for 90 species are similar. This conclusion has been confirmed by a direct calculation of the amino acid frequencies for each of the 90 species (data not shown here for saving ...
... amino acid frequencies for different species is small, too. In other words, the distributions of frequencies of amino acids for 90 species are similar. This conclusion has been confirmed by a direct calculation of the amino acid frequencies for each of the 90 species (data not shown here for saving ...
Biochemical Aspects of Digestion of Lipids
... Absorption of Lipids by Intestinal Mucosal Cells Mixed micelles*: Disc-shaped clusters of amphipathic lipids. Arranged with their hydrophobic groups on the inside and their hydrophilic groups on the outside. Micelle includes end products of lipid digestion, bile salts and fatso ...
... Absorption of Lipids by Intestinal Mucosal Cells Mixed micelles*: Disc-shaped clusters of amphipathic lipids. Arranged with their hydrophobic groups on the inside and their hydrophilic groups on the outside. Micelle includes end products of lipid digestion, bile salts and fatso ...
ch_02_lecture_outline_b
... • Convert strong (completely dissociated) acids or bases into weak (slightly dissociated) ones • Carbonic acid-bicarbonate system ...
... • Convert strong (completely dissociated) acids or bases into weak (slightly dissociated) ones • Carbonic acid-bicarbonate system ...
Document
... • Convert strong (completely dissociated) acids or bases into weak (slightly dissociated) ones • Carbonic acid-bicarbonate system ...
... • Convert strong (completely dissociated) acids or bases into weak (slightly dissociated) ones • Carbonic acid-bicarbonate system ...
Energy
... of atoms in molecules Rearrangement of atoms will either store or release energy chemical reaction = rearrangement of atoms ...
... of atoms in molecules Rearrangement of atoms will either store or release energy chemical reaction = rearrangement of atoms ...
Molecular Docking Studies of Isorhamnetin from Corchorus olitorius
... 5% to 7% in urban sub-Saharan [8]. Type II diabetes (T2DM) is a group of metabolic disorders arising from impaired insulin action and insulin secretion, leading to hyperglycemia. T2DM destroys many of the body system, most especially blood vessels [4]. T2DM may be in part as a result of increased he ...
... 5% to 7% in urban sub-Saharan [8]. Type II diabetes (T2DM) is a group of metabolic disorders arising from impaired insulin action and insulin secretion, leading to hyperglycemia. T2DM destroys many of the body system, most especially blood vessels [4]. T2DM may be in part as a result of increased he ...
AP Biology Chapter 8 Introduction to Metabolism Guided Notes
... The Activation Energy Barrier • Every chemical reaction between molecules involves bond ___________ and bond __________ • The initial energy needed to start a chemical reaction is called the free energy of activation, or ____________________________ • Activation energy is often supplied in the form ...
... The Activation Energy Barrier • Every chemical reaction between molecules involves bond ___________ and bond __________ • The initial energy needed to start a chemical reaction is called the free energy of activation, or ____________________________ • Activation energy is often supplied in the form ...
Nutrition: Protein
... T he combination of protein and carbohydrate, consumed as soon as possible after exercise, is better than either protein or carbohydrate alone for promoting muscle recovery from exercise. ...
... T he combination of protein and carbohydrate, consumed as soon as possible after exercise, is better than either protein or carbohydrate alone for promoting muscle recovery from exercise. ...
Bio-fermentation Technology to Improve Efficiency of Swine Nutrition*
... fermentation. Briefly, sugars from sugar cane are extracted and used as a substrate for fermentation by microorganisms specifically selected for synthesizing targeting amino acids. After these microorganisms excrete targeting amino acids to broth, drying process and filtration are followed to obtain ...
... fermentation. Briefly, sugars from sugar cane are extracted and used as a substrate for fermentation by microorganisms specifically selected for synthesizing targeting amino acids. After these microorganisms excrete targeting amino acids to broth, drying process and filtration are followed to obtain ...
IOSR Journal of Pharmacy and Biological Sciences (IOSR-JPBS) e-ISSN: 2278-3008.
... change may be a result of changes in the activity of enzymes. The activity of plant polyphenoloxidases can cause enzymatic browning of fruits and vegetables and render food unfit for consumption due to formation of benzoquinone. Proteases degrade polypeptides and facilitate seed germination, leaf se ...
... change may be a result of changes in the activity of enzymes. The activity of plant polyphenoloxidases can cause enzymatic browning of fruits and vegetables and render food unfit for consumption due to formation of benzoquinone. Proteases degrade polypeptides and facilitate seed germination, leaf se ...
Document
... Hold samples. Await histology. Remember to ask for fat stains as important indicators of metabolic disease (e.g. fatty acid oxidation defects). Remember Guthrie cards are kept by National Testing Centre for organic acid analysis etc (via Tandem Mass Spectrometry). It is also possible to do DNA studi ...
... Hold samples. Await histology. Remember to ask for fat stains as important indicators of metabolic disease (e.g. fatty acid oxidation defects). Remember Guthrie cards are kept by National Testing Centre for organic acid analysis etc (via Tandem Mass Spectrometry). It is also possible to do DNA studi ...
Intro to Carbon-based Molecules: Organic Chemistry
... Cell membrane with proteins & phospholipids Copyright Cmassengale ...
... Cell membrane with proteins & phospholipids Copyright Cmassengale ...
Human carboxylesterase 1: from drug metabolism to drug discovery
... alcohol are abused together [8]. When the active site of hCE1 is occupied by either naloxone or homatropine in our crystal structures, there is little room for compounds larger than water to enter the active site via the top of the catalytic gorge. We have proposed that for hCE1 to be able to transe ...
... alcohol are abused together [8]. When the active site of hCE1 is occupied by either naloxone or homatropine in our crystal structures, there is little room for compounds larger than water to enter the active site via the top of the catalytic gorge. We have proposed that for hCE1 to be able to transe ...
Induced-fit of the peptidyl-transferase center of the
... peptide bond formation with full-length substrates, the accommodation step (during which the 3' acceptor arm of an A-site tRNA moves into the PTC) must be excluded from the chemical step, a (still unresolved) difficulty at the origin of some controversy in the literature (Bieling et al. 2006; Johans ...
... peptide bond formation with full-length substrates, the accommodation step (during which the 3' acceptor arm of an A-site tRNA moves into the PTC) must be excluded from the chemical step, a (still unresolved) difficulty at the origin of some controversy in the literature (Bieling et al. 2006; Johans ...
Biosynthesis
Biosynthesis (also called biogenesis or anabolism) is a multi-step, enzyme-catalyzed process where substrates are converted into more complex products in living organisms. In biosynthesis, simple compounds are modified, converted into other compounds, or joined together to form macromolecules. This process often consists of metabolic pathways. Some of these biosynthetic pathways are located within a single cellular organelle, while others involve enzymes that are located within multiple cellular organelles. Examples of these biosynthetic pathways include the production of lipid membrane components and nucleotides.The prerequisite elements for biosynthesis include: precursor compounds, chemical energy (e.g. ATP), and catalytic enzymes which may require coenzymes (e.g.NADH, NADPH). These elements create monomers, the building blocks for macromolecules. Some important biological macromolecules include: proteins, which are composed of amino acid monomers joined via peptide bonds, and DNA molecules, which are composed of nucleotides joined via phosphodiester bonds.