PhD Thesis - Cox Group
... an organism and when metabolized they liberate large quantities of ATP. They are essential components of phospholipids and play a basic structural role in assembly of the cell membrane.31 Fatty acids are synthesized from simple building blocks particularly the two carbon containing acetate in the fo ...
... an organism and when metabolized they liberate large quantities of ATP. They are essential components of phospholipids and play a basic structural role in assembly of the cell membrane.31 Fatty acids are synthesized from simple building blocks particularly the two carbon containing acetate in the fo ...
Glycogen Metabolism
... Pyridoxal phosphate (PLP) is held at the active site by a Schiff base linkage, formed by reaction of the aldehyde of PLP with the e-amino group of a lysine residue. ...
... Pyridoxal phosphate (PLP) is held at the active site by a Schiff base linkage, formed by reaction of the aldehyde of PLP with the e-amino group of a lysine residue. ...
kbook or W METABOLIC DISEASE
... these essential nutrients in more detail. You may already be able to list some food sources rich in these nutrients, in this course we will also explore the functions of nutrients in the body and how much of each nutrient you need to keep your body running smoothly. In addition to maintaining specif ...
... these essential nutrients in more detail. You may already be able to list some food sources rich in these nutrients, in this course we will also explore the functions of nutrients in the body and how much of each nutrient you need to keep your body running smoothly. In addition to maintaining specif ...
Improvement of Aspergillus nidulans penicillin production by
... Next, a cyclase converts the tripeptide into a ring structure, isopenicillin N (IPN), the first intermediate with antibiotic activity. The enzyme requires Fe2 þ , molecular oxygen and ascorbate. The enzyme is called IPN synthase and is encoded by ipnA (pcbC). Finally, in A. nidulans and P. chrysogenu ...
... Next, a cyclase converts the tripeptide into a ring structure, isopenicillin N (IPN), the first intermediate with antibiotic activity. The enzyme requires Fe2 þ , molecular oxygen and ascorbate. The enzyme is called IPN synthase and is encoded by ipnA (pcbC). Finally, in A. nidulans and P. chrysogenu ...
Hydrophobins from Aspergillus species cannot be clearly divided
... are located before or after the conserved cysteine spacing pattern. Beauvais et al. (2007) have classified AFUA_8G05890 and AFUA_5G01490 as hydrophobins. As AFUA_8G05890 has 11 cysteines, no signal sequence and both proteins lack the conserved cysteine pattern, we disregard these proteins as hydroph ...
... are located before or after the conserved cysteine spacing pattern. Beauvais et al. (2007) have classified AFUA_8G05890 and AFUA_5G01490 as hydrophobins. As AFUA_8G05890 has 11 cysteines, no signal sequence and both proteins lack the conserved cysteine pattern, we disregard these proteins as hydroph ...
Predicting DNA-binding sites of proteins from amino acid sequence
... Background: Understanding the molecular details of protein-DNA interactions is critical for deciphering the mechanisms of gene regulation. We present a machine learning approach for the identification of amino acid residues involved in protein-DNA interactions. Results: We start with a Naïve Bayes c ...
... Background: Understanding the molecular details of protein-DNA interactions is critical for deciphering the mechanisms of gene regulation. We present a machine learning approach for the identification of amino acid residues involved in protein-DNA interactions. Results: We start with a Naïve Bayes c ...
Purification and Characterization of Chorismate
... be removed by gel filtration and it was lost during ammonium sulfate precipitation. Chorismate synthase activity eluted at a conductivity of 9.7 msiemens/cm from DEAE-Sepharose, well separated from the bulk protein. Fractions containing chorismate synthase activity could then be applied directly to ...
... be removed by gel filtration and it was lost during ammonium sulfate precipitation. Chorismate synthase activity eluted at a conductivity of 9.7 msiemens/cm from DEAE-Sepharose, well separated from the bulk protein. Fractions containing chorismate synthase activity could then be applied directly to ...
Amendment Determination 2007
... Treatment of type 2 diabetes, in combination with insulin, in a patient: (a) whose glycosylated haemoglobin (HbA1c) prior to initiation of insulin is greater than 7%, despite treatment with rosiglitazone maleate and at least 1 other oral anti-diabetic agent; or (b) in the case of patients who have c ...
... Treatment of type 2 diabetes, in combination with insulin, in a patient: (a) whose glycosylated haemoglobin (HbA1c) prior to initiation of insulin is greater than 7%, despite treatment with rosiglitazone maleate and at least 1 other oral anti-diabetic agent; or (b) in the case of patients who have c ...
Ribosomal Stalk Protein L12: Structure, Function and
... two RNAs, 23S rRNA (~2900 nucleotides) and 5S rRNA (~120 nucleotides) along with 33 proteins. The small subunit (30S) contains only one RNA component called the 16S rRNA (~1500 nucleotides) and about 21 proteins. Eukaryotic ribosomes are larger and much more complex than the prokaryotic ribosomes. T ...
... two RNAs, 23S rRNA (~2900 nucleotides) and 5S rRNA (~120 nucleotides) along with 33 proteins. The small subunit (30S) contains only one RNA component called the 16S rRNA (~1500 nucleotides) and about 21 proteins. Eukaryotic ribosomes are larger and much more complex than the prokaryotic ribosomes. T ...
Structural and mechanistic studies of enolase George H Reed
... Some of the several acidic and basic residues that line the active site are highlighted in the view of the Mg2+-enolase complex shown in Figure 4 [15]. An earlier structure of the substrate/product complex was obtained by soaking substrate into crystals of Mg2+-enolase at p H 6 in 3M(NH4)zSO4 [16]. ...
... Some of the several acidic and basic residues that line the active site are highlighted in the view of the Mg2+-enolase complex shown in Figure 4 [15]. An earlier structure of the substrate/product complex was obtained by soaking substrate into crystals of Mg2+-enolase at p H 6 in 3M(NH4)zSO4 [16]. ...
Late events of translation initiation in bacteria: a kinetic analysis
... used to monitor the conformational changes during initial binding of the EF-Tu ternary complex, codon recognition and release of aa-tRNA from EF-Tu followed by accommodation in the A-site (Figure 3B). Fluorescence changes of the GTP derivative, mant-GTP, were used to monitor the GTPase activation of ...
... used to monitor the conformational changes during initial binding of the EF-Tu ternary complex, codon recognition and release of aa-tRNA from EF-Tu followed by accommodation in the A-site (Figure 3B). Fluorescence changes of the GTP derivative, mant-GTP, were used to monitor the GTPase activation of ...
Glycogen Metabolism
... with α(14) glycosidic linkage This is repeated until a short linear glucose polymer (glycogen primer) with α(14) glycosidic linkages is built up on Glycogenin. ...
... with α(14) glycosidic linkage This is repeated until a short linear glucose polymer (glycogen primer) with α(14) glycosidic linkages is built up on Glycogenin. ...
Reprint
... II (MPO-Fe4+-OH) and can oxidize AH by one-electron transfer with formation of A•-radicals (reaction 3, Fig. 1) [12, 16, 19]. Compound II is catalytically inactive in hypohalous acid formation, but, like Compound I, it can cause one-electron oxidation of substrate (AH) with regeneration of the nativ ...
... II (MPO-Fe4+-OH) and can oxidize AH by one-electron transfer with formation of A•-radicals (reaction 3, Fig. 1) [12, 16, 19]. Compound II is catalytically inactive in hypohalous acid formation, but, like Compound I, it can cause one-electron oxidation of substrate (AH) with regeneration of the nativ ...
Quantitative Analysis of the Kinetics of End
... hydrolysis can proceed with no evident dissociation of RecA monomers (Neuendorf & Cox, 1986; Shan et al., 1997; Shan & Cox, 1996). A simple model arises. ATP hydrolysis occurs everywhere, resulting in dissociation only for monomers at the disassembling end, and occurring with a probability that is s ...
... hydrolysis can proceed with no evident dissociation of RecA monomers (Neuendorf & Cox, 1986; Shan et al., 1997; Shan & Cox, 1996). A simple model arises. ATP hydrolysis occurs everywhere, resulting in dissociation only for monomers at the disassembling end, and occurring with a probability that is s ...
General introduction - University of Amsterdam
... One of the main differences between C. albicans and the other described pathogenic fungi is that, in contrast with an environmental habitat and an inhalation route of infection of the latter, the normal habitat of C. albicans is the human body. C. albicans resides in the gastrointestinal tract and or ...
... One of the main differences between C. albicans and the other described pathogenic fungi is that, in contrast with an environmental habitat and an inhalation route of infection of the latter, the normal habitat of C. albicans is the human body. C. albicans resides in the gastrointestinal tract and or ...
1 High resolution metabolomics with acyl
... Thioester compounds containing coenzyme A (acyl-CoA) are key metabolites in intermediary metabolism. The most prominent of which is acetyl-CoA whose levels regulate critical cellular processes such as energy metabolism, protein acetylation, lipid synthesis and catabolism, and even autophagy (1-4). O ...
... Thioester compounds containing coenzyme A (acyl-CoA) are key metabolites in intermediary metabolism. The most prominent of which is acetyl-CoA whose levels regulate critical cellular processes such as energy metabolism, protein acetylation, lipid synthesis and catabolism, and even autophagy (1-4). O ...
patrick_tb_ch16
... Dihydropteroate is the product from this enzyme catalysed reaction. L-glutamic acid is added to dihydropteroate to form dihydrofolate. Neither are mimicked by the sulfonamides. The sulfonamides are derivatives of para-aminosulfonic acid and so this is not mimicked either. Page reference: 385 a. Dihy ...
... Dihydropteroate is the product from this enzyme catalysed reaction. L-glutamic acid is added to dihydropteroate to form dihydrofolate. Neither are mimicked by the sulfonamides. The sulfonamides are derivatives of para-aminosulfonic acid and so this is not mimicked either. Page reference: 385 a. Dihy ...
Biosynthesis
Biosynthesis (also called biogenesis or anabolism) is a multi-step, enzyme-catalyzed process where substrates are converted into more complex products in living organisms. In biosynthesis, simple compounds are modified, converted into other compounds, or joined together to form macromolecules. This process often consists of metabolic pathways. Some of these biosynthetic pathways are located within a single cellular organelle, while others involve enzymes that are located within multiple cellular organelles. Examples of these biosynthetic pathways include the production of lipid membrane components and nucleotides.The prerequisite elements for biosynthesis include: precursor compounds, chemical energy (e.g. ATP), and catalytic enzymes which may require coenzymes (e.g.NADH, NADPH). These elements create monomers, the building blocks for macromolecules. Some important biological macromolecules include: proteins, which are composed of amino acid monomers joined via peptide bonds, and DNA molecules, which are composed of nucleotides joined via phosphodiester bonds.