The Utilization of Magnesium by Certain Gram
... Inocula (0.1 ml.) for experiments with the Gram-negative organisms were taken from fully grown cultures in media that contained initially about 0.5 pg. Mg2+/ml. In these cultures the Mg2+was utilized completely; the culture fluid contained no residual Mg2+.As shown in the Results section, however, t ...
... Inocula (0.1 ml.) for experiments with the Gram-negative organisms were taken from fully grown cultures in media that contained initially about 0.5 pg. Mg2+/ml. In these cultures the Mg2+was utilized completely; the culture fluid contained no residual Mg2+.As shown in the Results section, however, t ...
CYP 2D6 Polymorphism
... Of the super-family of all cytochromes, the following families were confirmed in humans: CYP 1-5, 7, 8, 11, 17, 19, 21, 24, 26, 27, 39, 46, 51 ...
... Of the super-family of all cytochromes, the following families were confirmed in humans: CYP 1-5, 7, 8, 11, 17, 19, 21, 24, 26, 27, 39, 46, 51 ...
Developement of strategies for the isotopic labeling of methyl
... permits the suppression of spurious correlations, especially harmful for structural studies based on detection/analysis of nOes. To make an efficient use of the obtained high quality NMR spectra using this protocol, assignment of the methyl groups signals is mandatory. Two strategies were then propo ...
... permits the suppression of spurious correlations, especially harmful for structural studies based on detection/analysis of nOes. To make an efficient use of the obtained high quality NMR spectra using this protocol, assignment of the methyl groups signals is mandatory. Two strategies were then propo ...
Biochemical characterization of the initial steps of the Kennedy
... 5 min before the addition of ethanolamine or choline (0.1 mM). Direct EK and CK activity assays were performed by assessing the production of PtdEtn or PtdCho, using a modified method of Kim et al. [16]. Briefly, 2 μg of purified protein was incubated with a reaction mixture (total volume of 50 μl) ...
... 5 min before the addition of ethanolamine or choline (0.1 mM). Direct EK and CK activity assays were performed by assessing the production of PtdEtn or PtdCho, using a modified method of Kim et al. [16]. Briefly, 2 μg of purified protein was incubated with a reaction mixture (total volume of 50 μl) ...
Title of Document: HIGH-THROUGHPUT TIME-SERIES
... Research (TIGR) in Prof. John Quackenbush’s Lab. I would like to thank Mr. Ben Woodard of University of Maryland-Bio Scale-Up facility for allowing us to have access to their lab, house our GC-MS during the intermediate period of time and use some of the equipments required for our research. I woul ...
... Research (TIGR) in Prof. John Quackenbush’s Lab. I would like to thank Mr. Ben Woodard of University of Maryland-Bio Scale-Up facility for allowing us to have access to their lab, house our GC-MS during the intermediate period of time and use some of the equipments required for our research. I woul ...
Enzyme Mechanisms - Research Centers
... water-soluble coenzymes are derived from vitamins—typically B vitamins Typically the dietary form can be converted by a fairly short metabolic pathway into the coenzyme form, e.g. ...
... water-soluble coenzymes are derived from vitamins—typically B vitamins Typically the dietary form can be converted by a fairly short metabolic pathway into the coenzyme form, e.g. ...
Nucleotide sequence of the Tn10 encoded tetracycline resistance
... from Tn10 and gives the araino acid composition for the 43.3 kDa protein. The codon usage is clearly non-random and rather different from other E. coll proteins [19]. Very striking is the use of UUU 22 times out of 28 codons for Phe. A similar observation has been made for the transposon Tn3 genes [ ...
... from Tn10 and gives the araino acid composition for the 43.3 kDa protein. The codon usage is clearly non-random and rather different from other E. coll proteins [19]. Very striking is the use of UUU 22 times out of 28 codons for Phe. A similar observation has been made for the transposon Tn3 genes [ ...
Product Data Sheet
... Creatine plays a vital role in energy production by recycling ATP (adenosine triphosphate) in muscles. ATP is the body’s major molecule that produces “explosive” chemical energy within cells and is also known as “molecular currency” since it transfers energy within all cells of the body. Approximate ...
... Creatine plays a vital role in energy production by recycling ATP (adenosine triphosphate) in muscles. ATP is the body’s major molecule that produces “explosive” chemical energy within cells and is also known as “molecular currency” since it transfers energy within all cells of the body. Approximate ...
Biological ontologies for human functional annotation and
... model of cell signaling reactions in human. ... use of the GENIA ontology is to provide a basis for integrated view of multiple databases“ ...
... model of cell signaling reactions in human. ... use of the GENIA ontology is to provide a basis for integrated view of multiple databases“ ...
Production and Utilization of Acetate in Mammals
... of acetyl-CoA synthetase (EC 6.2.1.1) and acetyl-CoA hydrolase (EC 3.1.2.1) were assayed in tissues from rats and sheep. In addition, the concentrations of acetate in blood and liver were measured, as well as the rates of acetate production by tissue slices and mitochondrial fractions of these tissu ...
... of acetyl-CoA synthetase (EC 6.2.1.1) and acetyl-CoA hydrolase (EC 3.1.2.1) were assayed in tissues from rats and sheep. In addition, the concentrations of acetate in blood and liver were measured, as well as the rates of acetate production by tissue slices and mitochondrial fractions of these tissu ...
H. Draw a dipeptide from two amino acids
... compound and a brief statement as to why you think your answer is reasonable. 17. In general, what are the potential problems associated with a strict vegetarian diet? 18. How many ketone structures can be drawn that are isomeric with the aldehyde pentanal? 19. Aldehydes and ketones have lower boili ...
... compound and a brief statement as to why you think your answer is reasonable. 17. In general, what are the potential problems associated with a strict vegetarian diet? 18. How many ketone structures can be drawn that are isomeric with the aldehyde pentanal? 19. Aldehydes and ketones have lower boili ...
Internal Assessment: Fermentation Biology Higher Level
... The sugar splitting proceeds efficiently in aerobic or anaerobic environments. Glycolysis is the metabolic pathway that is common to all organisms on Earth. (Miller, & Levine, 2006) However, when oxygen is not present, the process of glycolysis is combined with different pathways. When combining the ...
... The sugar splitting proceeds efficiently in aerobic or anaerobic environments. Glycolysis is the metabolic pathway that is common to all organisms on Earth. (Miller, & Levine, 2006) However, when oxygen is not present, the process of glycolysis is combined with different pathways. When combining the ...
CHRYSAORA QUINQUECIRRHA) Research Article
... Furthermore, collagen is used as a moisturizer in cosmetic creams [14]. Moisturizers are employed for the therapy of so-called ‘dry skin’. Lipids work by the principle of occlusion, whereas humectants, such as collagen, attract water in the stratum corneum. In contrast to low molecular weight humect ...
... Furthermore, collagen is used as a moisturizer in cosmetic creams [14]. Moisturizers are employed for the therapy of so-called ‘dry skin’. Lipids work by the principle of occlusion, whereas humectants, such as collagen, attract water in the stratum corneum. In contrast to low molecular weight humect ...
Enzyme Inhibition and Bioapplications
... proposed on proteolysis in isolated lysosomes to establish the mechanism of degradation and proteolysis inhibition. Further, role of lysosomal enzymes is proposed for liver regeneration and recovery after nitroimidazole treatment. The emerging state of art is presented on role of enzyme inhibition i ...
... proposed on proteolysis in isolated lysosomes to establish the mechanism of degradation and proteolysis inhibition. Further, role of lysosomal enzymes is proposed for liver regeneration and recovery after nitroimidazole treatment. The emerging state of art is presented on role of enzyme inhibition i ...
Journal of Bacteriology
... The hypothesis is put forward that the amount of this murein precursor is regulated by feedback inhibition. A considerable amount of information is available concerning the structure (24) and synthesis (11, 22) of the murein of Escherichia coli. The two murein precursors, uridine diphosphate-N-acety ...
... The hypothesis is put forward that the amount of this murein precursor is regulated by feedback inhibition. A considerable amount of information is available concerning the structure (24) and synthesis (11, 22) of the murein of Escherichia coli. The two murein precursors, uridine diphosphate-N-acety ...
Mitochondria as a Pharmacological Target
... very active metabolically, such as muscles, liver, brain, and cardiac and skeletal muscles, contain the largest number of mitochondria and are most susceptible to drugs acting on mitochondria and to mitochondrial pathologies. In major mammalian tissues, 80 to 90% of ATP is generated by mitochondria ...
... very active metabolically, such as muscles, liver, brain, and cardiac and skeletal muscles, contain the largest number of mitochondria and are most susceptible to drugs acting on mitochondria and to mitochondrial pathologies. In major mammalian tissues, 80 to 90% of ATP is generated by mitochondria ...
Phospholipids of some marine microalgae: Identification, antivirus
... 3- Extraction and determination of total lipids Total lipids of marine algae (10 g) were extracted with 100 ml methanol: chloroform solvent (2:1, v/v) mixture [12]. After filtration, the mixture was evaporated at 40oC to a minimum volume (15 ml) and dried under N2. Then, the total lipids content wer ...
... 3- Extraction and determination of total lipids Total lipids of marine algae (10 g) were extracted with 100 ml methanol: chloroform solvent (2:1, v/v) mixture [12]. After filtration, the mixture was evaporated at 40oC to a minimum volume (15 ml) and dried under N2. Then, the total lipids content wer ...
Chapter 4 - University of Amsterdam
... products of peroxisomal β-oxidation, i.e. shortened fatty acids and acetyl units to the mitochondria. Additional (related) roles of carnitine include modulation of the acylCoA/CoA ratio, storage of energy (in the form of acetyl-carnitine) and excretion of poorly metabolizable, toxic, acyl groups (4) ...
... products of peroxisomal β-oxidation, i.e. shortened fatty acids and acetyl units to the mitochondria. Additional (related) roles of carnitine include modulation of the acylCoA/CoA ratio, storage of energy (in the form of acetyl-carnitine) and excretion of poorly metabolizable, toxic, acyl groups (4) ...
Glycation by Ascorbic Acid Causes Loss of Activity of Ribulose
... Glycation is a process whereby sugar molecules form a covalent adduct with protein amino groups. In this study, we used ascorbic acid (AsA) as a glycating agent and purified cucumber (Cucumis sativus L.) ribulose-1,5-bisphosphate carboxylase/oxygenase (Rubisco) as a model protein in chloroplast tiss ...
... Glycation is a process whereby sugar molecules form a covalent adduct with protein amino groups. In this study, we used ascorbic acid (AsA) as a glycating agent and purified cucumber (Cucumis sativus L.) ribulose-1,5-bisphosphate carboxylase/oxygenase (Rubisco) as a model protein in chloroplast tiss ...
... Pgc1α gene Adeno-associated vector serotype 1 carrying Pparδ gene Mixture of an adeno-associated vector 1 the Pgc1α gene and an adeno1 carrying the rat Mixture of an adeno-associated vector 1 the Pgc1α gene and an adeno1 carrying the Pparδ gene American Diabetes Association Adenosine diphosphate Pro ...
FAT/CD36 is located on the outer mitochondrial membrane
... of mitochondrial fatty-acid transport, in which FAT/CD36 is positioned on the OMM, upstream of long-chain acyl-CoA synthetase, thereby contributing to the regulation of mitochondrial fatty-acid transport. We further support this model by providing evidence that FAT/CD36 is not located in mitochondri ...
... of mitochondrial fatty-acid transport, in which FAT/CD36 is positioned on the OMM, upstream of long-chain acyl-CoA synthetase, thereby contributing to the regulation of mitochondrial fatty-acid transport. We further support this model by providing evidence that FAT/CD36 is not located in mitochondri ...
Regulation of Phenylalanine and Tyrosine
... hyperbolic with K , values of 0.31 r n M for chorismate and 0.015 mM for prephenate, respectively. The substrate saturation curve of the complexed prephenate dehydratase I was sigmoid; a Km value of 0-18 mM was calculated for prephenate. Chorismate mutase, prephenate dehydratase and prephenate dehyd ...
... hyperbolic with K , values of 0.31 r n M for chorismate and 0.015 mM for prephenate, respectively. The substrate saturation curve of the complexed prephenate dehydratase I was sigmoid; a Km value of 0-18 mM was calculated for prephenate. Chorismate mutase, prephenate dehydratase and prephenate dehyd ...
Metabolism
Metabolism (from Greek: μεταβολή metabolē, ""change"") is the set of life-sustaining chemical transformations within the cells of living organisms. These enzyme-catalyzed reactions allow organisms to grow and reproduce, maintain their structures, and respond to their environments. The word metabolism can also refer to all chemical reactions that occur in living organisms, including digestion and the transport of substances into and between different cells, in which case the set of reactions within the cells is called intermediary metabolism or intermediate metabolism.Metabolism is usually divided into two categories: catabolism, the breaking down of organic matter by way of cellular respiration, and anabolism, the building up of components of cells such as proteins and nucleic acids. Usually, breaking down releases energy and building up consumes energy.The chemical reactions of metabolism are organized into metabolic pathways, in which one chemical is transformed through a series of steps into another chemical, by a sequence of enzymes. Enzymes are crucial to metabolism because they allow organisms to drive desirable reactions that require energy that will not occur by themselves, by coupling them to spontaneous reactions that release energy. Enzymes act as catalysts that allow the reactions to proceed more rapidly. Enzymes also allow the regulation of metabolic pathways in response to changes in the cell's environment or to signals from other cells.The metabolic system of a particular organism determines which substances it will find nutritious and which poisonous. For example, some prokaryotes use hydrogen sulfide as a nutrient, yet this gas is poisonous to animals. The speed of metabolism, the metabolic rate, influences how much food an organism will require, and also affects how it is able to obtain that food.A striking feature of metabolism is the similarity of the basic metabolic pathways and components between even vastly different species. For example, the set of carboxylic acids that are best known as the intermediates in the citric acid cycle are present in all known organisms, being found in species as diverse as the unicellular bacterium Escherichia coli and huge multicellular organisms like elephants. These striking similarities in metabolic pathways are likely due to their early appearance in evolutionary history, and their retention because of their efficacy.