Multipotent stem cells from the dorsal aorta
... later in life during postnatal growth or regeneration. Because of their origin, they would remain capable of differentiating into other tissue types when naturally or experimentally relocated into a different tissue. To prove this hypothesis, we transplanted quail or mouse embryonic aorta into chick ...
... later in life during postnatal growth or regeneration. Because of their origin, they would remain capable of differentiating into other tissue types when naturally or experimentally relocated into a different tissue. To prove this hypothesis, we transplanted quail or mouse embryonic aorta into chick ...
S tem cells and cardiac disorders: an appraisal
... GATA-4 and NKX2.5, in mouse embryos containing null alleles of MEF2c, cardiomyocyte differentiation was disrupted, the ventricle failed to form and a subset of cardiac muscle genes (including ANF) were not expressed [15]. While these cardiac transcription factors have been the focus of much research ...
... GATA-4 and NKX2.5, in mouse embryos containing null alleles of MEF2c, cardiomyocyte differentiation was disrupted, the ventricle failed to form and a subset of cardiac muscle genes (including ANF) were not expressed [15]. While these cardiac transcription factors have been the focus of much research ...
of Limb Morphogenesis in a Model System
... begun to exhibit the cartilage tissue found in the stage 25 proximal mesoblast. Since a population of cells in this stage of the limb are not homogeneous with respect to cytodifferentiation as are chondrocytes from stage 30, or morphogenetic expression, as are stage 19 cells, they must contain eithe ...
... begun to exhibit the cartilage tissue found in the stage 25 proximal mesoblast. Since a population of cells in this stage of the limb are not homogeneous with respect to cytodifferentiation as are chondrocytes from stage 30, or morphogenetic expression, as are stage 19 cells, they must contain eithe ...
tetraploidy checkpoint - The Journal of Cell Biology
... coverslip with a diamond scribe, the drug was washed out and time-lapse observations were resumed. None of the cells exiting mitosis in the presence of the drug showed any furrowing activity (Video 1). Nevertheless, all 20 binucleates followed went through mitosis by 18 h after drug removal (Fig. 1 ...
... coverslip with a diamond scribe, the drug was washed out and time-lapse observations were resumed. None of the cells exiting mitosis in the presence of the drug showed any furrowing activity (Video 1). Nevertheless, all 20 binucleates followed went through mitosis by 18 h after drug removal (Fig. 1 ...
pGLO LAB EDITED by ME
... did not already have, then the bacteria will be “transformed” and will exhibit new traits. The molecular biologist begins by treating the bacteria cells with a solution of calcium chloride in order to make cells that will more readily take up DNA—these are competent cells. The positive charges of th ...
... did not already have, then the bacteria will be “transformed” and will exhibit new traits. The molecular biologist begins by treating the bacteria cells with a solution of calcium chloride in order to make cells that will more readily take up DNA—these are competent cells. The positive charges of th ...
Effect of lithium and sodium cations on the
... Monovalent cations are very important in yeast metabolism. However, high internal concentrations of Na+ or its analogue Li+ are generally toxic to cells [1]. Lithium is highly toxic to yeast when grown in galactose medium, mainly because phosphoglucomutase, a key enzyme of galactose metabolism, is i ...
... Monovalent cations are very important in yeast metabolism. However, high internal concentrations of Na+ or its analogue Li+ are generally toxic to cells [1]. Lithium is highly toxic to yeast when grown in galactose medium, mainly because phosphoglucomutase, a key enzyme of galactose metabolism, is i ...
BHS 116.2: Physiology II Date: 2/6/13 Notetaker: Stephanie Cullen
... o Metabolizing fats Chylomicrons enter systemic circulation right from the GI tract via the lymph system Primary source of lipids after a meal Eventually go to the liver and are processed In b/t meals, tissues need fat so use lipoproteins VLDL: rich in triglycerides LDL: primary lipid pa ...
... o Metabolizing fats Chylomicrons enter systemic circulation right from the GI tract via the lymph system Primary source of lipids after a meal Eventually go to the liver and are processed In b/t meals, tissues need fat so use lipoproteins VLDL: rich in triglycerides LDL: primary lipid pa ...
bop8e_chapter03 REVISED W16 Part 2
... Microtubule Arrays and the Cell Cycle • Changes in the distribution of microtubules during the cell cycle and cell wall formation during cytokinesis • (a) During interphase, and in enlarging and differentiating cells, the microtubules lie just inside the plasma membrane • (b) Just before prophase, ...
... Microtubule Arrays and the Cell Cycle • Changes in the distribution of microtubules during the cell cycle and cell wall formation during cytokinesis • (a) During interphase, and in enlarging and differentiating cells, the microtubules lie just inside the plasma membrane • (b) Just before prophase, ...
Why don`t Cells Grow Indefinitely Lab
... 1. Cut out the three cell models. Fold and glue together all sides of each model. You will have three structures that resemble open boxes, as in Figure 1. Imagine that each cell model has a sixth side and is a closed box. These models represent a cell at three different stages of growth. The younges ...
... 1. Cut out the three cell models. Fold and glue together all sides of each model. You will have three structures that resemble open boxes, as in Figure 1. Imagine that each cell model has a sixth side and is a closed box. These models represent a cell at three different stages of growth. The younges ...
Slide 1
... The crucial step in the staining process is the decolorizing step. The most accepted theory relies on the fact that the PPG is found in layers and the stain molecules are trapped within the many layers of the GP CW when they form the complex with the mordant Iodine ...
... The crucial step in the staining process is the decolorizing step. The most accepted theory relies on the fact that the PPG is found in layers and the stain molecules are trapped within the many layers of the GP CW when they form the complex with the mordant Iodine ...
Low-cost `plastic` solar cells: a dream becoming a reality Alan Heeger
... the two components on a length scale of a few nanometres, roughly a hundred angstroms (1 angstrom is equal to 0.1 nanometre). A conceptual sketch of this nanomorphology is shown in Figure 2 a. As demonstrated in Figure 2 b, this remarkable nano-structure can already be constructed. How was it formed ...
... the two components on a length scale of a few nanometres, roughly a hundred angstroms (1 angstrom is equal to 0.1 nanometre). A conceptual sketch of this nanomorphology is shown in Figure 2 a. As demonstrated in Figure 2 b, this remarkable nano-structure can already be constructed. How was it formed ...
Reovirus for the treatment of acute myeloid leukaemia
... fibroblasts increased expression of both CD54 and CD55 was observed. The efficacy of Cavatak virotherapy was increased after co-culture with CD40L positive cells. CLL cell lines are susceptible to Cavatak virotherapy but human primary cells were resistant. In our model of lymph node resistant diseas ...
... fibroblasts increased expression of both CD54 and CD55 was observed. The efficacy of Cavatak virotherapy was increased after co-culture with CD40L positive cells. CLL cell lines are susceptible to Cavatak virotherapy but human primary cells were resistant. In our model of lymph node resistant diseas ...
Bioorthogonal chemical imaging of metabolic activities in live
... The mammalian hippocampus is an active region of brain metabolism involved in learning, memory formation, and has strong implications in many neurological diseases. Organotypic hippocampal slice culture provides a complex tissue model that mimics the structure and activity in vivo with multiple cell ...
... The mammalian hippocampus is an active region of brain metabolism involved in learning, memory formation, and has strong implications in many neurological diseases. Organotypic hippocampal slice culture provides a complex tissue model that mimics the structure and activity in vivo with multiple cell ...
Ultrastructural and physiological changes in root cells of Sorghum
... expect structural changes in these cells, which may explain the low salt tolerance of the Sorghum species. At the tissue level, roots show a broad spectrum of structural adaptations to environmental changes. NaCl salinity or Fe deficiency can, for example, lead to reduced growth and to a shorter elo ...
... expect structural changes in these cells, which may explain the low salt tolerance of the Sorghum species. At the tissue level, roots show a broad spectrum of structural adaptations to environmental changes. NaCl salinity or Fe deficiency can, for example, lead to reduced growth and to a shorter elo ...
as a PDF
... cell mass of blastocyst [day 3.5 (E3.5) preimplantation embryo] and maintained as undifferentiated stem cells on a feeder layer of mouse embryonic fibroblasts (MEFs). Undifferentiated ES cells can also be maintained without a feeder layer, if they are cultured on gelatin-coated dishes in a serum-con ...
... cell mass of blastocyst [day 3.5 (E3.5) preimplantation embryo] and maintained as undifferentiated stem cells on a feeder layer of mouse embryonic fibroblasts (MEFs). Undifferentiated ES cells can also be maintained without a feeder layer, if they are cultured on gelatin-coated dishes in a serum-con ...
NIPS April 1998 notebook
... membrane from the basolateral surface into the cell where it is processed. Whereas some of this basolateral membrane is used for apical secretion, particularly of the sIgA complex, a significant amount is cycled back to the basolateral surface after intracellular processing. This basolateral membran ...
... membrane from the basolateral surface into the cell where it is processed. Whereas some of this basolateral membrane is used for apical secretion, particularly of the sIgA complex, a significant amount is cycled back to the basolateral surface after intracellular processing. This basolateral membran ...
Finals – Study Guide
... that is flexible and interacts with the environment. Only certain cells have a cell wall, which is rigid and provides shape and support to cells. 15. They enable plants to convert solar energy into energy-rich molecules that cells can use. 16. endoplasmic reticulum 17. mitochondrion ...
... that is flexible and interacts with the environment. Only certain cells have a cell wall, which is rigid and provides shape and support to cells. 15. They enable plants to convert solar energy into energy-rich molecules that cells can use. 16. endoplasmic reticulum 17. mitochondrion ...
3. Bacterial Cytology
... often much thicker than the cell itself. This slide illustrates a technique called ‘negative staining’. In this technique, the stain binds to the bacterial cells and to the residual culture medium adhering to the microscope slide , but not to the capsules themselves. The result is that the capsules ...
... often much thicker than the cell itself. This slide illustrates a technique called ‘negative staining’. In this technique, the stain binds to the bacterial cells and to the residual culture medium adhering to the microscope slide , but not to the capsules themselves. The result is that the capsules ...
- Annals of Gastroenterology
... - Thank you for the great suggestion. Per your advice, we have now elaborated more fully on the background and aim of this paper in our introduction section. The introduction section has been rewritten as follows: “Polyethylene glycol (PEG) 3350 and Electrolytes for Oral Solution (GoLYTELY®, Braintr ...
... - Thank you for the great suggestion. Per your advice, we have now elaborated more fully on the background and aim of this paper in our introduction section. The introduction section has been rewritten as follows: “Polyethylene glycol (PEG) 3350 and Electrolytes for Oral Solution (GoLYTELY®, Braintr ...
Unit III - Cells - Lesson Module
... Each individual cell exists in a fluid environment, and the cytoplasm within the cell also has a fluid environment. The presence of a liquid makes it possible for substances (such as nutrients, oxygen, and waste products) to move into and out of the cell. A cell membrane is semipermeable (selectivel ...
... Each individual cell exists in a fluid environment, and the cytoplasm within the cell also has a fluid environment. The presence of a liquid makes it possible for substances (such as nutrients, oxygen, and waste products) to move into and out of the cell. A cell membrane is semipermeable (selectivel ...
Negative Controls of Cell Proliferation: Human
... \B). Addition of DHT to either female or male CD serumsupplemented media generated a biphasic proliferative response (Fig. \B); the peak was reached at 3 x 10~'" M. Concentrations above 3 x 10"'" M DHT resulted in progressively lower cell yields. As a result of this type of experiment, heretofore, w ...
... \B). Addition of DHT to either female or male CD serumsupplemented media generated a biphasic proliferative response (Fig. \B); the peak was reached at 3 x 10~'" M. Concentrations above 3 x 10"'" M DHT resulted in progressively lower cell yields. As a result of this type of experiment, heretofore, w ...
digestion of carbohydrates
... rehydration fluid allows uptake of sodium to replenish body sodium chloride which is deficient due to diarrhea 2. Uniport System Releases Glucose into Blood The same intestinal epithelial cells have a different transport mechanism on the membrane facing capillaries. Intestinal cells release glucose ...
... rehydration fluid allows uptake of sodium to replenish body sodium chloride which is deficient due to diarrhea 2. Uniport System Releases Glucose into Blood The same intestinal epithelial cells have a different transport mechanism on the membrane facing capillaries. Intestinal cells release glucose ...
Mechanisms of endothelial cell swelling from lactacidosis studied in
... contribute to pHi regulation; among these, the Na⫹dependent Cl⫺/HCO3⫺ exchanger (NCBE) has been found activated in acidosis in bovine aortic endothelium (4). In addition, Na⫹-HCO3⫺ cotransport is activated in corneal endothelium (1, 2, 10). Na ⫹-independent Cl⫺/HCO3⫺ exchange (CBE) is known to maint ...
... contribute to pHi regulation; among these, the Na⫹dependent Cl⫺/HCO3⫺ exchanger (NCBE) has been found activated in acidosis in bovine aortic endothelium (4). In addition, Na⫹-HCO3⫺ cotransport is activated in corneal endothelium (1, 2, 10). Na ⫹-independent Cl⫺/HCO3⫺ exchange (CBE) is known to maint ...
Modelling and parameter estimation of bacterial growth with
... Measure the lag (LN) by means of the fitted bi-phasic linear function generated by N initial cells: y(t) = y0 + max( µ(t-LN), 0) y0=lnN We call the obtained L-value as the “geometrical” lag, due to its definition. If N=1, we arrive at the single cell lag time, L1. It is not the same as the physiolog ...
... Measure the lag (LN) by means of the fitted bi-phasic linear function generated by N initial cells: y(t) = y0 + max( µ(t-LN), 0) y0=lnN We call the obtained L-value as the “geometrical” lag, due to its definition. If N=1, we arrive at the single cell lag time, L1. It is not the same as the physiolog ...
Tissue engineering
Tissue engineering is the use of a combination of cells, engineering and materials methods, and suitable biochemical and physicochemical factors to improve or replace biological functions. While it was once categorized as a sub-field of biomaterials, having grown in scope and importance it can be considered as a field in its own right.While most definitions of tissue engineering cover a broad range of applications, in practice the term is closely associated with applications that repair or replace portions of or whole tissues (i.e., bone, cartilage, blood vessels, bladder, skin, muscle etc.). Often, the tissues involved require certain mechanical and structural properties for proper functioning. The term has also been applied to efforts to perform specific biochemical functions using cells within an artificially-created support system (e.g. an artificial pancreas, or a bio artificial liver). The term regenerative medicine is often used synonymously with tissue engineering, although those involved in regenerative medicine place more emphasis on the use of stem cells or progenitor cells to produce tissues.