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18. plant growth - Development of e
18. plant growth - Development of e

... Typical growth regions in plants are the apices of shoot and root. Such growing regions are known as apical meristems, primary meristems or regions of primary growth. These apical meristems are responsible for the increase in length, differentiation of various appendages and formation of plant tissu ...
PDF
PDF

... genes, designated as Hro-hes (accession# AY144625) and Tru-hes (accession# AY144624). Each fragment contains an intron (126 and 324 bp, respectively) at a site that is conserved with respect to other organisms (Fig. 2). For Tru-hes, we obtained only a partial sequence within the bHLH domain. For Hro ...
2 StainsInMicro
2 StainsInMicro

... bacterial cells. For this reason, using basic stains may not be the best way to discern the cell morphology (shape). Acidic Stains Acidic stains have negatively charged chromatophores that are repelled by the bacterial cell envelope. They are usually mixed together with a small amount of liquid cult ...
Dishevelled 2 signaling promotes self
Dishevelled 2 signaling promotes self

... new insights into the disease.Several groups have identified a sub-population of stemlike, cancer cells in brain tumors, using biomarker analysis and culturing techniques similar to those used to characterize normal neural stem cells. These cells demonstrate a significant tumor-initiating ability, ...
Microtubule-Dependent Regulation of α2B Adrenergic Receptors in
Microtubule-Dependent Regulation of α2B Adrenergic Receptors in

... antibody was from the Berkley Antibody (Richmond, CA); Cy-3conjugated donkey anti-mouse IgG was from Jackson ImmunoResearch (West Grove, PA); monoclonal anti-b-tubulin was from Amersham (Arlington Heights, IL); and rhodamine-conjugated phalloidin was from Molecular Probes (Eugene, OR). Colchicine an ...
Mitochondria in Lymphocytes of Normal and
Mitochondria in Lymphocytes of Normal and

... occasionally fail to take the dye, while some fading of the stain occurs." Although Wiseman's paper is the only one dealing with the lymphocyte in particular, many others have shown that large numbers of mitochondria are present in young blood cells (2, 5). As part of a comparative cytological study ...
Electron Microscope Observations of Brucella abortus
Electron Microscope Observations of Brucella abortus

... therefore that the known differences between slant-grown and cell-grown brucellas, if they involve changes in surface structures, are associated with modifications which are not detectable with the electron microscope. The changes in serum sensitivity in brucellas may be more closely related to diff ...
Prokaryotic
Prokaryotic

... 3. Eukaryotic—complex, contains a nucleus, many organelles 4. Prokaryotic—small, simple, no organelles are ...
Primordial germ cells in the mouse embryo during
Primordial germ cells in the mouse embryo during

... examined some embryos at this stage, both as an additional control for the specificity of our staining procedure and to follow the pattern of ALP staining into the period of somitogenesis. In the whole-mount staining of presomite to 4-somite embryos, a dense population of ALP-positive cells was demo ...
Homeostasis, Transport, and Bioenergetics
Homeostasis, Transport, and Bioenergetics

... A. Diffusion is the movement of molecules from an area of higher concentration to one of lower concentration by random molecular motion. B. Diffusion is the movement of molecules from an area of lower concentration to one of higher concentration by random molecular motion. C. Diffusion is the moveme ...
Oxygen accessibility and iron levels are critical
Oxygen accessibility and iron levels are critical

... Laboratories) and Lee’s medium14 and tested in a microdilution test. Drug solutions (100 mL), 90 mL of medium and 10 mL of semisynchronized cells (107 cells/mL) were added to the wells of a 96well plate. Final drug concentrations were between 0.0037 and 128 mg/L. Controls included medium without any ...
ochromonas malhamensis
ochromonas malhamensis

... ribonuclease (E .C . 2 .7.7 .16 ribonucleate nucleotido-2'-transferase) were done on Ochromonas cultures in the same experimental treatments and under identical assay conditions as the cytochemical study . During starvation, the acid hydrolase specific activities were consistently twice those found ...
Sample preparation for Transmission electron microscopy (TEM
Sample preparation for Transmission electron microscopy (TEM

... plus a monitor, or on a layer of imaging plate, or to be detected by a sensor such as a CCD camera. ...
CH 01 FINAL
CH 01 FINAL

... arise only by the division of preexisting cells. Cell division is the only path to immortality. Nondividing cells can live for as long as a hundred years, but they always eventually die. Viruses are the one apparent exception to the cell theory, but since they can replicate only inside cells, their ...
A Cell Type-specific Constitutive Point Mutant of the
A Cell Type-specific Constitutive Point Mutant of the

... domains of hGMRa (283 amino acids). A full description of the templates and primers used is available upon request. Extracellular truncations of mGMRa were generated by PCR on the pRUFNeo/FmGMRa construct with primers designed to amplify the entire construct except for the desired extracellular sequ ...
to a prolonged period of sucrose deprivation
to a prolonged period of sucrose deprivation

... the cell dry weight and total fatty acids (Fig. 5). The increase in the cell dry weight was attributable to a rapid accumulation of sucrose in the vacuolar reservoir and starch in plastids (not shown), whereas the increase in total cell fatty acids was attributable to the synthesis of new cytoplasmi ...
Cell and Membrane Practice - Hatboro
Cell and Membrane Practice - Hatboro

... Some cells, such as human nerve and muscle cells, contain many more mitochondria than do other cells, such as skin cells. Why do some cells have more mitochondria than others? A. ...
FROM THE LEAVES OF PHYLLOSTACHYS BAMBUSOIDES
FROM THE LEAVES OF PHYLLOSTACHYS BAMBUSOIDES

... In order to evaluate the role of ethyl acetate fraction (PB-EtAC) obtained from the Phyllostachys bambusoides leaves in the modulation of immune responses, detailed studies were carried out using a panel of in vivo assays. Oral administration of PB-EtAC (50–200 mg/Kg) stimulated the IgM and IgG titr ...
The CENP-O complex requirement varies among different cell types
The CENP-O complex requirement varies among different cell types

... Chicken DT40 cells with KO of each CENP-O complex protein were viable, although they exhibited subtle mitotic defects (Minoshima et al. 2005; Okada et al. 2006; Hori et al. 2008b). However, the requirement for the CENP-O complex proteins for the viability of other cell types remained uncertain. Thus ...
the specificity and stability of the triton
the specificity and stability of the triton

... These conclusions, that a suitable extraction with a non-ionic detergent can separate the cell into two useful fractions and that the cytoskeletal frameworks so produced faithfully reflect the spatial properties of the living cell, will be readily accepted by most. Indeed, to some, the accumulated b ...
Platelet releasate increases the proliferation and
Platelet releasate increases the proliferation and

... to obtain a final concentration of 1000  106 platelets/ml. This represented a 491% increase over the average physiological concentration of collected platelets (214  37  106 platelets/ml). The platelets were activated with 1 National Institute of Health (NIH) U/ml of bovine thrombin (Sigma) for 3 ...
Laboratory Diagnosis of Viral Diseases
Laboratory Diagnosis of Viral Diseases

... CMV (pp65 antigenaemia test) ...
Symplasmic fields in the tunica of the shoot
Symplasmic fields in the tunica of the shoot

... how these gradients and hypothetical signal networks are positioned in the cellular matrix of the AM. Importantly, the proliferating AM restricts pattern formation to its periphery and maintains an undifferentiated centre (e.g. Sawhney et al., 1981; Steeves and Sussex, 1989) which suggests the prese ...
WLC4 91-92
WLC4 91-92

... o In same cell scenario, if another user is much closer to the base than the desired user, its adjacent channel signal can cause significant interference. o Assume the ratio of distance from the two sources to the base (D1/D2), is equal to 20, then SIR =20-n which for n=4 is equal to -52dB. o If Rx ...
Symplasmic fields in the tunica of the shoot
Symplasmic fields in the tunica of the shoot

... how these gradients and hypothetical signal networks are positioned in the cellular matrix of the AM. Importantly, the proliferating AM restricts pattern formation to its periphery and maintains an undifferentiated centre (e.g. Sawhney et al., 1981; Steeves and Sussex, 1989) which suggests the prese ...
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Tissue engineering



Tissue engineering is the use of a combination of cells, engineering and materials methods, and suitable biochemical and physicochemical factors to improve or replace biological functions. While it was once categorized as a sub-field of biomaterials, having grown in scope and importance it can be considered as a field in its own right.While most definitions of tissue engineering cover a broad range of applications, in practice the term is closely associated with applications that repair or replace portions of or whole tissues (i.e., bone, cartilage, blood vessels, bladder, skin, muscle etc.). Often, the tissues involved require certain mechanical and structural properties for proper functioning. The term has also been applied to efforts to perform specific biochemical functions using cells within an artificially-created support system (e.g. an artificial pancreas, or a bio artificial liver). The term regenerative medicine is often used synonymously with tissue engineering, although those involved in regenerative medicine place more emphasis on the use of stem cells or progenitor cells to produce tissues.
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