Identification of trichoplein, a novel keratin filament
... Lowicryl K4M resin (Polysciences, Warrington, PA) according to the manufacturer’s protocol. Immunolabeling was carried out as described (Kachi et al., 1999). Immunohistochemistry Mouse (liver, brain, skeletal muscle, blood vessels of skeletal muscle, and hair follicle) and rat (small intestine) tiss ...
... Lowicryl K4M resin (Polysciences, Warrington, PA) according to the manufacturer’s protocol. Immunolabeling was carried out as described (Kachi et al., 1999). Immunohistochemistry Mouse (liver, brain, skeletal muscle, blood vessels of skeletal muscle, and hair follicle) and rat (small intestine) tiss ...
Dendritic cells in humans—from fetus to adult
... significant advancements in in vitro clonal assays, xenotransplantation techniques and increasing recognition of the importance of studies in patients with hematological disorders, the factors regulating myeloid development in humans are gradually being revealed [reviewed by Doulatov et al. (56)]. A ...
... significant advancements in in vitro clonal assays, xenotransplantation techniques and increasing recognition of the importance of studies in patients with hematological disorders, the factors regulating myeloid development in humans are gradually being revealed [reviewed by Doulatov et al. (56)]. A ...
Advantages over Mechanical Dissociation of Cells
... (LCST) at 31 8C. Above the LCST (i.e., at the cell culture temperature of 37 8C), the surfaces are observed to be more hydrophobic (as observed by water contact angle), and many cell types will adhere and proliferate. Below the LCST (i.e., at room temperature) the surface rapidly hydrates, and cell ...
... (LCST) at 31 8C. Above the LCST (i.e., at the cell culture temperature of 37 8C), the surfaces are observed to be more hydrophobic (as observed by water contact angle), and many cell types will adhere and proliferate. Below the LCST (i.e., at room temperature) the surface rapidly hydrates, and cell ...
cell organelles
... In the diagram above, molecules are crossing the plasma membrane in the opposite direction of diffusion. Here, molecules are moving from a low concentration to a high concentration. Imagine you are in a crowd of people all going in one direction and you are going the opposite way. It would be easier ...
... In the diagram above, molecules are crossing the plasma membrane in the opposite direction of diffusion. Here, molecules are moving from a low concentration to a high concentration. Imagine you are in a crowd of people all going in one direction and you are going the opposite way. It would be easier ...
Standard PDF - Wiley Online Library
... for self-antigens escape, tolerance is maintained by peripheral tolerance mechanisms in lymph nodes, tonsil, or spleen. Peripheral tolerance can occur through different mechanisms like induction of anergy, induction of apoptosis, or suppression through regulatory T cells. If peripheral tolerance is ...
... for self-antigens escape, tolerance is maintained by peripheral tolerance mechanisms in lymph nodes, tonsil, or spleen. Peripheral tolerance can occur through different mechanisms like induction of anergy, induction of apoptosis, or suppression through regulatory T cells. If peripheral tolerance is ...
CLONING HUMAN BEINGS The Science of Animal Cloning Commissioned Paper
... not to be the case. Early experiments showed that nuclei of adult cells fused with fertilized eggs did not undergo nuclear swelling or nuclear division (Graham 1969). A careful series of experiments by McGrath and Solter in the mid 1980s showed that nuclei could be successfully exchanged between zyg ...
... not to be the case. Early experiments showed that nuclei of adult cells fused with fertilized eggs did not undergo nuclear swelling or nuclear division (Graham 1969). A careful series of experiments by McGrath and Solter in the mid 1980s showed that nuclei could be successfully exchanged between zyg ...
isolation and characterization of a cell wall
... C. monoica Strehlow used in this study was obtained from the University of Texas Culture Collection of Algae (UTEX 220). A wall-defective mutant clone 10A3-1 (referred to hereafter as cw-1) was obtained from UTEX 220 after mutagenesis with UV light. For genetic analysis the cw-1 mutant was crossed t ...
... C. monoica Strehlow used in this study was obtained from the University of Texas Culture Collection of Algae (UTEX 220). A wall-defective mutant clone 10A3-1 (referred to hereafter as cw-1) was obtained from UTEX 220 after mutagenesis with UV light. For genetic analysis the cw-1 mutant was crossed t ...
Polarity of Water Transport across Epidermal Cell
... giving a lower average coefficient of variation (15.2%, 5.8%, and 19.0% for half-time, cell volume [Vo], and hydraulic conductivity [Lp], respectively) than the PC method (25.4%, 22.0%, and 24.2%, respectively). Vo as determined from PC and PR tests was 1.1 to 2.7 nL and in the range of optically est ...
... giving a lower average coefficient of variation (15.2%, 5.8%, and 19.0% for half-time, cell volume [Vo], and hydraulic conductivity [Lp], respectively) than the PC method (25.4%, 22.0%, and 24.2%, respectively). Vo as determined from PC and PR tests was 1.1 to 2.7 nL and in the range of optically est ...
Parallels between tissue repair and embryo morphogenesis
... Building tissues and organs during embryogenesis involves a series of exquisite morphogenetic episodes that are driven by the marriage of regulated proliferative events with a series of precisely orchestrated tissue contractions, foldings and migrations. Slowly, a miniature model of the adult form r ...
... Building tissues and organs during embryogenesis involves a series of exquisite morphogenetic episodes that are driven by the marriage of regulated proliferative events with a series of precisely orchestrated tissue contractions, foldings and migrations. Slowly, a miniature model of the adult form r ...
Epitaxially Lifted Off (ELO) Light Weight, Low Cost GaAs Solar Cells
... based materials over the entire substrate area with excellent success. ELO can be used to realize ultra light high efficiency solar cells for both space and terrestrial applications. The ELO process also provides the ability to reuse the substrate numerous times. This provides tremendous benefits in ...
... based materials over the entire substrate area with excellent success. ELO can be used to realize ultra light high efficiency solar cells for both space and terrestrial applications. The ELO process also provides the ability to reuse the substrate numerous times. This provides tremendous benefits in ...
Midterm I Mock Exam Key
... A) Sexual reproduction results in less genetic variability than asexual reproduction. B) Asexual reproduction may be an advantage to organisms that are isolated from potential mates. C) Sexual reproduction results in offspring that are genetically identical to the parent. D) Asexual reproduction typ ...
... A) Sexual reproduction results in less genetic variability than asexual reproduction. B) Asexual reproduction may be an advantage to organisms that are isolated from potential mates. C) Sexual reproduction results in offspring that are genetically identical to the parent. D) Asexual reproduction typ ...
From indolence to persistence
... Bacterial persisters are dormant cells that arise heterogeneously in an antibiotic-susceptible population during entry into stationary phase. These cells are able to tolerate antibiotic treatment and are often involved in chronic and recurrent infections, but the signals that trigger their formation ...
... Bacterial persisters are dormant cells that arise heterogeneously in an antibiotic-susceptible population during entry into stationary phase. These cells are able to tolerate antibiotic treatment and are often involved in chronic and recurrent infections, but the signals that trigger their formation ...
Bud Formation by the Yeast Saccharomyces
... Cells bearing the cdc4-6 allele were studied, since such cells at the nonpermissive temperature were found to produce budlike structures from several sites on the cell surface ; this property made multiple budding events easy to discern . Exponentially growing cultures of the cdc4-6 mutant strains G ...
... Cells bearing the cdc4-6 allele were studied, since such cells at the nonpermissive temperature were found to produce budlike structures from several sites on the cell surface ; this property made multiple budding events easy to discern . Exponentially growing cultures of the cdc4-6 mutant strains G ...
CONTRIBUTION OF STEM CELLS AND DIFFERENTIATED CELLS
... leukaemia. The cells that are capable of initiating human acute myeloid leukaemia when transplanted into immunocompromised mice have a phenotype that is similar to haematopoietic stem cells35; however, it is also possible to generate myeloid leukaemia, in a more benign form, by targeting restricted ...
... leukaemia. The cells that are capable of initiating human acute myeloid leukaemia when transplanted into immunocompromised mice have a phenotype that is similar to haematopoietic stem cells35; however, it is also possible to generate myeloid leukaemia, in a more benign form, by targeting restricted ...
It is essential for students to know the three major tenets of the cell
... In the development of most multicellular organisms, a single cell (fertilized egg) gives rise to many different types of cells, each with a different structure and corresponding function. ○ The fertilized egg gives rise to a large number of cells through cell division, but the process of cell divi ...
... In the development of most multicellular organisms, a single cell (fertilized egg) gives rise to many different types of cells, each with a different structure and corresponding function. ○ The fertilized egg gives rise to a large number of cells through cell division, but the process of cell divi ...
Communicating Research to the General Public
... diagnosis would have been to figure out whether you had a bacterial infection. If so, the doctor would have prescribed an antibiotic dose for 3-7 days. Antibiotics are “wonder drugs” that have been used to treat bacterial infections since the 1940s. Penicillin, arguably the most popular antibiotic, ...
... diagnosis would have been to figure out whether you had a bacterial infection. If so, the doctor would have prescribed an antibiotic dose for 3-7 days. Antibiotics are “wonder drugs” that have been used to treat bacterial infections since the 1940s. Penicillin, arguably the most popular antibiotic, ...
cells
... describe and give examples of chemical reactions required to sustain life (…role of enzymes) describe the relationship between structure and function explain how homeostasis is maintained within living systems; ...
... describe and give examples of chemical reactions required to sustain life (…role of enzymes) describe the relationship between structure and function explain how homeostasis is maintained within living systems; ...
Synaptic basis for intense thalamocortical activation of feedforward
... The response of most FS cells to thalamocortical input began as a strong excitatory conductance (Ge), followed 1–3 ms later by an even stronger inhibitory conductance (Gi). This is illustrated for an example cell in Figure 2a. Thus, at depolarized holding potentials (between reversal potentials for ...
... The response of most FS cells to thalamocortical input began as a strong excitatory conductance (Ge), followed 1–3 ms later by an even stronger inhibitory conductance (Gi). This is illustrated for an example cell in Figure 2a. Thus, at depolarized holding potentials (between reversal potentials for ...
DNA Strand Breaks Produced by Etoposide
... multidrug-resistant MCF-7 cell line indicating that other biochemical factors, such as activation of VP-16, are also involved in drug resistance and suggesting that the resistance is multifactorial. ...
... multidrug-resistant MCF-7 cell line indicating that other biochemical factors, such as activation of VP-16, are also involved in drug resistance and suggesting that the resistance is multifactorial. ...
Relation of Interferon Production to the Limited Replication of
... high p.f.u.: HA ratio. Thus, the limited growth of NDV in L cells could be circumvented by neutralizing the IFN with specific antiserum. However, the effect of antiserum was either not appreciable (single cycle growth) or not distinct (multiple cycle growth) if neutralization was only partial (Table ...
... high p.f.u.: HA ratio. Thus, the limited growth of NDV in L cells could be circumvented by neutralizing the IFN with specific antiserum. However, the effect of antiserum was either not appreciable (single cycle growth) or not distinct (multiple cycle growth) if neutralization was only partial (Table ...
Docosahexaenoic acid improves endogen antioxidant
... suggested as a general measurement cell injury. The aim of this study, therefore, was to analyze the effect of DHA supplementation on the modulation of the redox cellular balance in retinal cells. Reactive oxygen species (ROS) are intimately involved in the oxidative damage of retina and are used by ...
... suggested as a general measurement cell injury. The aim of this study, therefore, was to analyze the effect of DHA supplementation on the modulation of the redox cellular balance in retinal cells. Reactive oxygen species (ROS) are intimately involved in the oxidative damage of retina and are used by ...
Racial differences in B cell receptor signaling pathway activation
... measured in viable B cells (defined as amine aqua negative, forward scatter/side scatter low, CD20+, c-poly (ADP-ribose) polymerase (c-PARP) negative cells, Figure 1) at several time points following modulation with anti-IgD in PBMCs collected from 10 healthy donors (Table 1). The “Fold” metric (see ...
... measured in viable B cells (defined as amine aqua negative, forward scatter/side scatter low, CD20+, c-poly (ADP-ribose) polymerase (c-PARP) negative cells, Figure 1) at several time points following modulation with anti-IgD in PBMCs collected from 10 healthy donors (Table 1). The “Fold” metric (see ...
cytochemical localization of acid phosphatases in euglena gracilis
... that some constitutive acid phosphatase activity is associated with the paramylum bodies. The appearance of a lead sulfide precipitate in the absence of added substrate, probably indicates the existence of an endogenous source of phosphate in the paramylum bodies. In induced cell preparations treate ...
... that some constitutive acid phosphatase activity is associated with the paramylum bodies. The appearance of a lead sulfide precipitate in the absence of added substrate, probably indicates the existence of an endogenous source of phosphate in the paramylum bodies. In induced cell preparations treate ...
Par-complex proteins promote proliferative
... (Takahashi et al., 1995; Chenn and McConnell, 1995; Haydar et al., 2003; Noctor et al., 2004; Haubensak et al., 2004). Par3 knockdown decreases clone size in vitro The continuing decrease in Par3 expression during development prompted us to examine a potential role for this complex in VZ progenitors ...
... (Takahashi et al., 1995; Chenn and McConnell, 1995; Haydar et al., 2003; Noctor et al., 2004; Haubensak et al., 2004). Par3 knockdown decreases clone size in vitro The continuing decrease in Par3 expression during development prompted us to examine a potential role for this complex in VZ progenitors ...
Tissue engineering
Tissue engineering is the use of a combination of cells, engineering and materials methods, and suitable biochemical and physicochemical factors to improve or replace biological functions. While it was once categorized as a sub-field of biomaterials, having grown in scope and importance it can be considered as a field in its own right.While most definitions of tissue engineering cover a broad range of applications, in practice the term is closely associated with applications that repair or replace portions of or whole tissues (i.e., bone, cartilage, blood vessels, bladder, skin, muscle etc.). Often, the tissues involved require certain mechanical and structural properties for proper functioning. The term has also been applied to efforts to perform specific biochemical functions using cells within an artificially-created support system (e.g. an artificial pancreas, or a bio artificial liver). The term regenerative medicine is often used synonymously with tissue engineering, although those involved in regenerative medicine place more emphasis on the use of stem cells or progenitor cells to produce tissues.