A1984SX34500001
... proposed, for example, that histamine's ability to inhibit its own release might constitute the basis of an inhibitory feedback loop that would serve to inhibit the extent or intensity of allergic responses. Subsequent experiments (summarized in reference 2) provided suggestive evidence in favor of ...
... proposed, for example, that histamine's ability to inhibit its own release might constitute the basis of an inhibitory feedback loop that would serve to inhibit the extent or intensity of allergic responses. Subsequent experiments (summarized in reference 2) provided suggestive evidence in favor of ...
RGD-dependent Linkage between Plant Cell Wall
... GRGESP (0.5 mg/ml). The growth curves in Fig. 3 monitored by wet weight indicate that cells grown either in the absence or presence of SPGDRG or GRGESP had normal doubling times of "~24-26 h. In marked contrast, these same cells cultured in the presence of GRGDSP had grown to 3--4 times their starti ...
... GRGESP (0.5 mg/ml). The growth curves in Fig. 3 monitored by wet weight indicate that cells grown either in the absence or presence of SPGDRG or GRGESP had normal doubling times of "~24-26 h. In marked contrast, these same cells cultured in the presence of GRGDSP had grown to 3--4 times their starti ...
1 Collaborative Control of Cell Cycle Progression by the
... with T7 promoter on the 5’ end and SP6 promoter on the 3’ end. The PCR product was gel purified, and 1ug of purified DNA template was used in an in vitro transcription reaction with T7 RNA polymerase. The in vitro transcription reaction was performed for 1 hour at 37C. At the end of the reaction, 10 ...
... with T7 promoter on the 5’ end and SP6 promoter on the 3’ end. The PCR product was gel purified, and 1ug of purified DNA template was used in an in vitro transcription reaction with T7 RNA polymerase. The in vitro transcription reaction was performed for 1 hour at 37C. At the end of the reaction, 10 ...
Approaches for Monitoring Nuclear Translation
... The nuclear membrane is the defining feature of eukaryotes. It divides the cell into two functionally specialized compartments, and it has been widely assumed that translation is restricted to only one: the cytoplasm. However, recent results suggest that some translation takes place in nuclei (1,2). ...
... The nuclear membrane is the defining feature of eukaryotes. It divides the cell into two functionally specialized compartments, and it has been widely assumed that translation is restricted to only one: the cytoplasm. However, recent results suggest that some translation takes place in nuclei (1,2). ...
Ordinal Position of Neurons in Cat Striate Cortex
... was exclusively associated with a single ordinal position. In particular, a large proportion of S-, Sh-, C-, and N-O and cone cells showed a monosynaptic input from the thalamus (groups M and Cl+,)). 5. For the three classes of cell, S, Sh, and C, with sufficient numbers in groups MY cl+n~ and D, a ...
... was exclusively associated with a single ordinal position. In particular, a large proportion of S-, Sh-, C-, and N-O and cone cells showed a monosynaptic input from the thalamus (groups M and Cl+,)). 5. For the three classes of cell, S, Sh, and C, with sufficient numbers in groups MY cl+n~ and D, a ...
Leaf initiation: the integration of growth and cell division
... division. For example, promotion of cell proliferation by overexpression of a cyclinD2 led to an increased growth rate but plant form was normal (Cockcoft et al., 2000). Repression of cell proliferation can lead to the generation of smaller plants (e.g., by overexpression of E2F/DP factors or CDK in ...
... division. For example, promotion of cell proliferation by overexpression of a cyclinD2 led to an increased growth rate but plant form was normal (Cockcoft et al., 2000). Repression of cell proliferation can lead to the generation of smaller plants (e.g., by overexpression of E2F/DP factors or CDK in ...
Fall/Winter 2014 Newsletter - Hickory Veterinary Hospital
... Chronic pain can lead to a significantly decreased quality of life for many pets. Sometimes this pain can be managed with medications, dietary supplements, and /or physical therapy, but other times, this is not possible. Often, owners are looking for a different approach to helping their pet feel be ...
... Chronic pain can lead to a significantly decreased quality of life for many pets. Sometimes this pain can be managed with medications, dietary supplements, and /or physical therapy, but other times, this is not possible. Often, owners are looking for a different approach to helping their pet feel be ...
Trypanosome TOR complex 2 functions in cytokinesis
... thus leading to cell mass accumulation. Metazoans seemed to be subjected to more complex regulation through the involvement of the insulin/insulin-like growth factor system, which couples TOR activation to energy levels, growth factors or variation in oxygen concentration in order to orchestrate ove ...
... thus leading to cell mass accumulation. Metazoans seemed to be subjected to more complex regulation through the involvement of the insulin/insulin-like growth factor system, which couples TOR activation to energy levels, growth factors or variation in oxygen concentration in order to orchestrate ove ...
A Mechanism for Asymmetric Cell Division Resulting in Proliferative
... AKT3). To do so, we obtained HCT116 colorectal cancer cells with adeno-associated virus (AAV)-mediated disruption of the AKT1 and AKT2 gene loci (i.e., AKT1/2/ cells; ref. 2). Importantly, AKT1/2/ cells do not express either AKT1 or AKT2, nor do they express AKT3, and thus survive and proliferat ...
... AKT3). To do so, we obtained HCT116 colorectal cancer cells with adeno-associated virus (AAV)-mediated disruption of the AKT1 and AKT2 gene loci (i.e., AKT1/2/ cells; ref. 2). Importantly, AKT1/2/ cells do not express either AKT1 or AKT2, nor do they express AKT3, and thus survive and proliferat ...
Role of bilateral zones of ingressing superficial cells
... substratum for mesodermal cell migration, in various species of amphibians. They found dense extracellular fibrils appearing just prior to the onset of gastrulation in the three species of urodele studied, but far fewer in the two anuran species looked at, possibly implying that migration plays diff ...
... substratum for mesodermal cell migration, in various species of amphibians. They found dense extracellular fibrils appearing just prior to the onset of gastrulation in the three species of urodele studied, but far fewer in the two anuran species looked at, possibly implying that migration plays diff ...
Intercellular communication in the early embryo of
... 50min after fertilization. The plane of cleavage is meridional and coincides with the plane of bilateral symmetry of the larva. The blastomeres are of equal size and according to Conklin's (1905) nomenclature they are termed AB2 and AB2. The second plane of cleavage is perpendicular to the first and ...
... 50min after fertilization. The plane of cleavage is meridional and coincides with the plane of bilateral symmetry of the larva. The blastomeres are of equal size and according to Conklin's (1905) nomenclature they are termed AB2 and AB2. The second plane of cleavage is perpendicular to the first and ...
Peroxiredoxin-controlled G-CSF signalling at the endoplasmic
... phagocytes the Nox system mainly serves to produce high levels of H2O2 for the so-called oxidative burst, a major weapon in host defence against bacteria. In non-phagocytic cells, H2O2 has long been considered as an unwanted by-product of cell metabolism, potentially hazardous because of the damagin ...
... phagocytes the Nox system mainly serves to produce high levels of H2O2 for the so-called oxidative burst, a major weapon in host defence against bacteria. In non-phagocytic cells, H2O2 has long been considered as an unwanted by-product of cell metabolism, potentially hazardous because of the damagin ...
Examining the link between chromosomal instability and aneuploidy
... maintain them during cell division is currently unknown. In some cases, the unusually high numbers of chromosomes are segregated faithfully, and the aneuploid karyotype displays little variation over time (Storchova and Pellman, 2004). However, in other cases, aneuploid cells are genetically unstabl ...
... maintain them during cell division is currently unknown. In some cases, the unusually high numbers of chromosomes are segregated faithfully, and the aneuploid karyotype displays little variation over time (Storchova and Pellman, 2004). However, in other cases, aneuploid cells are genetically unstabl ...
T Regulatory Cells 1 Inhibit a Th2
... clones, whereas OVA-specific IgG1 and IgG2a responses were not inhibited (Fig. 1). This specific effect of Tr1 clones on IgE production was completely reversed after injection of blocking antiIL-10R Abs, confirming the importance of this cytokine in the regulatory effect of Tr1 clones (17). In contr ...
... clones, whereas OVA-specific IgG1 and IgG2a responses were not inhibited (Fig. 1). This specific effect of Tr1 clones on IgE production was completely reversed after injection of blocking antiIL-10R Abs, confirming the importance of this cytokine in the regulatory effect of Tr1 clones (17). In contr ...
Probing the invasiveness of prostate cancer cells in a 3D
... Fig. 1 shows the high-aspect-ratio Tepuis constructed to ascertain three-dimensional invasiveness. The Tepuis have an aspect ratio of about 3∶1, because they are 100 μm wide and 320 μm high. Two prostate cancer cell lines were used in this study (16): PC-3 cells derived from bone marrow, which are h ...
... Fig. 1 shows the high-aspect-ratio Tepuis constructed to ascertain three-dimensional invasiveness. The Tepuis have an aspect ratio of about 3∶1, because they are 100 μm wide and 320 μm high. Two prostate cancer cell lines were used in this study (16): PC-3 cells derived from bone marrow, which are h ...
Mechanism of artificial transformation of E. coli with plasmid DNA
... naturally in many species such as Micrococcus, Haemophilus and Bacillus1,2; all these organisms have proteins on their exterior surface whose function is to bind to DNA in their environment and transport it into the cell. However, it is still a rare event for most bacteria to naturally take up DNA f ...
... naturally in many species such as Micrococcus, Haemophilus and Bacillus1,2; all these organisms have proteins on their exterior surface whose function is to bind to DNA in their environment and transport it into the cell. However, it is still a rare event for most bacteria to naturally take up DNA f ...
Cell polarity and tissue patterning in plants - Development
... An advantage of the "canalization hypothesis' is that it suggests various experimental approaches to cellular patterning (Sachs, 1981) and the one to be followed here is to search for additional cellular expressions of the same canalized controls of polarization. The 'canalization hypothesis' consid ...
... An advantage of the "canalization hypothesis' is that it suggests various experimental approaches to cellular patterning (Sachs, 1981) and the one to be followed here is to search for additional cellular expressions of the same canalized controls of polarization. The 'canalization hypothesis' consid ...
Document
... A cell is the basic structural and functional unit of all living things. The human body consists of trillions and trillions of cells. But cells are too small to see with the human eye. The invention of the microscope allowed scientists to discover that cells existed. In 1665, an English scientist na ...
... A cell is the basic structural and functional unit of all living things. The human body consists of trillions and trillions of cells. But cells are too small to see with the human eye. The invention of the microscope allowed scientists to discover that cells existed. In 1665, an English scientist na ...
Transport Within Cells
... the smallest living unit of matter. They are membrane covered structures that contains all of the materials necessary for life. You are composed of cells. Some living organisms are only 1 cell. They are called unicellular. There are both plants and animals that are unicellular. Most plants and anima ...
... the smallest living unit of matter. They are membrane covered structures that contains all of the materials necessary for life. You are composed of cells. Some living organisms are only 1 cell. They are called unicellular. There are both plants and animals that are unicellular. Most plants and anima ...
A natural anti-T-cell receptor monoclonal antibody protects against
... encephalitogenic T cells; and these T cells and antibodies protected from EAE when passively transferred into naïve mice. The mechanisms of action of regulatory anti-TCR T cells have been analyzed extensively and may involve anergy (Kumar et al., 1995), deletion (Madakamutil et al., 2003) or immune ...
... encephalitogenic T cells; and these T cells and antibodies protected from EAE when passively transferred into naïve mice. The mechanisms of action of regulatory anti-TCR T cells have been analyzed extensively and may involve anergy (Kumar et al., 1995), deletion (Madakamutil et al., 2003) or immune ...
intracellular accumulations
... 4. An abnormal exogenous substance is deposited and accumulates because the cell has neither the enzymatic machinery to degrade the substance nor the ability to transport it to other sites. (e.g. Accumulations of carbon or silica particles) ...
... 4. An abnormal exogenous substance is deposited and accumulates because the cell has neither the enzymatic machinery to degrade the substance nor the ability to transport it to other sites. (e.g. Accumulations of carbon or silica particles) ...
What the Distribution of Cell Lengths in the Root Meristem Does and
... A persistent controversy about the root meristem is whether all cells divide. Some authors have argued that cells exit the cell cycle anywhere within the meristem and have introduced the term ``proliferative fraction'' to account for this variable proportion of dividing cells (Clowes 1976). Values r ...
... A persistent controversy about the root meristem is whether all cells divide. Some authors have argued that cells exit the cell cycle anywhere within the meristem and have introduced the term ``proliferative fraction'' to account for this variable proportion of dividing cells (Clowes 1976). Values r ...
Molecular role of GATA binding protein 4 (GATA
... Methods: Streptozotocin-induced diabetic rats and H9c2 embryonic rat cardiomyocytes treated with a high concentration of glucose (a D-glucose concentration of 30 mM was used and cells were cultured for 24 hr) were used to examine the effect of hyperglycemia on GATA-4 accumulation in the nucleus. cTn ...
... Methods: Streptozotocin-induced diabetic rats and H9c2 embryonic rat cardiomyocytes treated with a high concentration of glucose (a D-glucose concentration of 30 mM was used and cells were cultured for 24 hr) were used to examine the effect of hyperglycemia on GATA-4 accumulation in the nucleus. cTn ...
1 Developing accurate models of the human airways 1 2 Marshall L
... basal cell population (1, 8). These cellular junctions form a tight but selective barrier in the paracellular space between epithelial cells, separating the lumen of the airways from the underlying tissue. Tight junctions are located closest to the lumen and form a bel ...
... basal cell population (1, 8). These cellular junctions form a tight but selective barrier in the paracellular space between epithelial cells, separating the lumen of the airways from the underlying tissue. Tight junctions are located closest to the lumen and form a bel ...
calcium homoestasis
... 1. Osteoblasts are the differentiated bone forming cells and secrete bone matrix on which Ca++ and PO precipitate. 2. Osteocytes, the mature bone cells are enclosed in bone matrix. 3. Osteoclasts is a large multinucleated cell derived from monocytes whose function is to resorb bone. • These cells ar ...
... 1. Osteoblasts are the differentiated bone forming cells and secrete bone matrix on which Ca++ and PO precipitate. 2. Osteocytes, the mature bone cells are enclosed in bone matrix. 3. Osteoclasts is a large multinucleated cell derived from monocytes whose function is to resorb bone. • These cells ar ...
Tissue engineering
Tissue engineering is the use of a combination of cells, engineering and materials methods, and suitable biochemical and physicochemical factors to improve or replace biological functions. While it was once categorized as a sub-field of biomaterials, having grown in scope and importance it can be considered as a field in its own right.While most definitions of tissue engineering cover a broad range of applications, in practice the term is closely associated with applications that repair or replace portions of or whole tissues (i.e., bone, cartilage, blood vessels, bladder, skin, muscle etc.). Often, the tissues involved require certain mechanical and structural properties for proper functioning. The term has also been applied to efforts to perform specific biochemical functions using cells within an artificially-created support system (e.g. an artificial pancreas, or a bio artificial liver). The term regenerative medicine is often used synonymously with tissue engineering, although those involved in regenerative medicine place more emphasis on the use of stem cells or progenitor cells to produce tissues.