Targeted disruption of the mouse E-Ras gene
... described elsewhere (Y. Tokuzawa et al., Mol. Cell. Bio., in press). pCAG-gw-IH was constructed as follows: cDNA of hygromycin resistance gene was amplified from pHPCAG with primers hyg.upper and hyg.lower. Additional PCR with primers attB1 and attB2 was performed and PCR product was inserted into p ...
... described elsewhere (Y. Tokuzawa et al., Mol. Cell. Bio., in press). pCAG-gw-IH was constructed as follows: cDNA of hygromycin resistance gene was amplified from pHPCAG with primers hyg.upper and hyg.lower. Additional PCR with primers attB1 and attB2 was performed and PCR product was inserted into p ...
Histone H3 phosphorylation is required for the initiation, but not
... To correlate the extent of H3 phosphorylation with OAinduced PCC, we exposed asynchronous cell populations to 0.5 µM OA for 2.5 hours. Under these conditions, over 70% of HeLa and IM cells appeared rounded up with compact nuclei characteristic of S-phase PCC. The nuclei of these cells displayed high ...
... To correlate the extent of H3 phosphorylation with OAinduced PCC, we exposed asynchronous cell populations to 0.5 µM OA for 2.5 hours. Under these conditions, over 70% of HeLa and IM cells appeared rounded up with compact nuclei characteristic of S-phase PCC. The nuclei of these cells displayed high ...
7-1 Life Is Cellular
... plants were made of cells. In 1839, Theodor Schwann stated that all animals were made of cells. In 1855, Rudolph Virchow concluded that new cells were created only from the reproduction of existing cells. These discoveries led to the cell theory. Slide 7 of 31 Copyright Pearson Prentice Hall ...
... plants were made of cells. In 1839, Theodor Schwann stated that all animals were made of cells. In 1855, Rudolph Virchow concluded that new cells were created only from the reproduction of existing cells. These discoveries led to the cell theory. Slide 7 of 31 Copyright Pearson Prentice Hall ...
BOSTON UNIVERSITY SCHOOL OF MEDICINE Thesis THE ROLE
... We know that new adult neurons are generated from neural stem cells or glial precursors (Gonzalez- Perez 2012), but there is still debate over which cells will give rise to new neurons. We know that many new neurons are born but only a select few will survive. It has been suggested that adult neurog ...
... We know that new adult neurons are generated from neural stem cells or glial precursors (Gonzalez- Perez 2012), but there is still debate over which cells will give rise to new neurons. We know that many new neurons are born but only a select few will survive. It has been suggested that adult neurog ...
LESSON—19 THE EXTERIOR OP THE CELL. A. Objectives
... B. recognition of the hormone insulin. C. recognition of self. D. recognition of a particle to be phagocytized. E. transport of glycine through a coupled channel. ...
... B. recognition of the hormone insulin. C. recognition of self. D. recognition of a particle to be phagocytized. E. transport of glycine through a coupled channel. ...
Role of Folylpolyglutamate Synthetase in the
... viding cultures (>4-fold higher than confluent cultures) was accompanied by an increase in extract FPGS activity (2.2-fold). The depletion of cellular folates produced comparable increases in both cellular methotrexate glutamylation and extract FPGS activity (approximately 1.8-fold). The addition of ...
... viding cultures (>4-fold higher than confluent cultures) was accompanied by an increase in extract FPGS activity (2.2-fold). The depletion of cellular folates produced comparable increases in both cellular methotrexate glutamylation and extract FPGS activity (approximately 1.8-fold). The addition of ...
Cell Cycle
... An example of an internal signal occurs at the M phase checkpoint In this case, anaphase does not begin if any kinetochores remain unattached to spindle microtubules Attachment of all of the kinetochores activates a regulatory complex, which then activates the enzyme separase Separase allow ...
... An example of an internal signal occurs at the M phase checkpoint In this case, anaphase does not begin if any kinetochores remain unattached to spindle microtubules Attachment of all of the kinetochores activates a regulatory complex, which then activates the enzyme separase Separase allow ...
Shroom3-mediated recruitment of Rho kinases to the apical cell
... by immunostaining for ZO-1, a tight-junction protein, appeared slightly curved and/or serrated (Fig. 1B). When Shrm-Full was expressed by removal of doxycycline, the apical junctions of the transfectants became tense and straightened (Fig. 1B). By contrast, expression of Shrm-ΔASD2 had no such effec ...
... by immunostaining for ZO-1, a tight-junction protein, appeared slightly curved and/or serrated (Fig. 1B). When Shrm-Full was expressed by removal of doxycycline, the apical junctions of the transfectants became tense and straightened (Fig. 1B). By contrast, expression of Shrm-ΔASD2 had no such effec ...
Shroom3-mediated recruitment of Rho kinases to the apical cell
... by immunostaining for ZO-1, a tight-junction protein, appeared slightly curved and/or serrated (Fig. 1B). When Shrm-Full was expressed by removal of doxycycline, the apical junctions of the transfectants became tense and straightened (Fig. 1B). By contrast, expression of Shrm-ΔASD2 had no such effec ...
... by immunostaining for ZO-1, a tight-junction protein, appeared slightly curved and/or serrated (Fig. 1B). When Shrm-Full was expressed by removal of doxycycline, the apical junctions of the transfectants became tense and straightened (Fig. 1B). By contrast, expression of Shrm-ΔASD2 had no such effec ...
Nonredox 5-Lipoxygenase Inhibitors Require Glutathione
... 1993). N-hydroxyureas and hydroxamates are weak redox active compounds and it is presumed that the 5-LO inhibitory action of these drugs might be related in part to these properties (Riendeau et al., 1991; Rouzer et al., 1991). The search for specific and highly potent nonredox 5-LO inhibitors led t ...
... 1993). N-hydroxyureas and hydroxamates are weak redox active compounds and it is presumed that the 5-LO inhibitory action of these drugs might be related in part to these properties (Riendeau et al., 1991; Rouzer et al., 1991). The search for specific and highly potent nonredox 5-LO inhibitors led t ...
University of Zurich - Institute of Molecular Life Sciences
... exchanger, actin, Rac1, Pak1 and CtBP1 but not heparan sulfate, which had been suggested to be involved in virus attachment to Chinese hamster ovary cells (99). These results mirror the infectious pathway into epithelial cells for the species B1 Ad3 (3). This is remarkable since Ad3 had been suggest ...
... exchanger, actin, Rac1, Pak1 and CtBP1 but not heparan sulfate, which had been suggested to be involved in virus attachment to Chinese hamster ovary cells (99). These results mirror the infectious pathway into epithelial cells for the species B1 Ad3 (3). This is remarkable since Ad3 had been suggest ...
Transplantation of porcine umbilical cord Matrix cells
... M.L. Weiss et al. / Experimental Neurology 182 (2003) 288 –299 ...
... M.L. Weiss et al. / Experimental Neurology 182 (2003) 288 –299 ...
I SEMESTER CYTOLOGY AND GENETICS
... The aim of this course is to provide an introduction to recombinant DNA technology. It helps the students to understand how the principles of molecular biology have been used to develop techniques in recombinant DNA technology. The objective of the course is to familiarize the student with the basic ...
... The aim of this course is to provide an introduction to recombinant DNA technology. It helps the students to understand how the principles of molecular biology have been used to develop techniques in recombinant DNA technology. The objective of the course is to familiarize the student with the basic ...
Get PDF file - Botanik in Bonn
... apparatus (GA), GA-based secretory vesicles, and ribosomes. It gains control over the original cell periphery-based structures, such as the actin cytoskeleton and endosomes. This provides the Energide with the tools necessary to regulate not only its own motility, the distribution of organelles and ...
... apparatus (GA), GA-based secretory vesicles, and ribosomes. It gains control over the original cell periphery-based structures, such as the actin cytoskeleton and endosomes. This provides the Energide with the tools necessary to regulate not only its own motility, the distribution of organelles and ...
Two-step process for photoreceptor formation inDrosophila
... outer PRCs, for instance in mis-expression experiments with rough or seven-up18,19. However, in these cases, the transformation occurs much earlier in the disc and concerns only R7 cells. Although this has not been addressed, we predict that, in this case, the projections of transformed R7 cells are ...
... outer PRCs, for instance in mis-expression experiments with rough or seven-up18,19. However, in these cases, the transformation occurs much earlier in the disc and concerns only R7 cells. Although this has not been addressed, we predict that, in this case, the projections of transformed R7 cells are ...
LvDelta induces mesoderm and endoderm
... Primary antibodies included 6a9 (Ettensohn and McClay, 1988) and 6e10 (Ingersoll, 1993) (both are markers for skeletogenic mesoderm), SMC2 and SMC1 (a blastocoelar cell marker and a marker specific for prospective secondary mesenchyme cells (SMCs) in the vegetal plate, respectively) (Hodor, 1998; Sw ...
... Primary antibodies included 6a9 (Ettensohn and McClay, 1988) and 6e10 (Ingersoll, 1993) (both are markers for skeletogenic mesoderm), SMC2 and SMC1 (a blastocoelar cell marker and a marker specific for prospective secondary mesenchyme cells (SMCs) in the vegetal plate, respectively) (Hodor, 1998; Sw ...
MCAS Review - Pittsfield Public Schools
... C. Explain how the structure of the cell membrane allows it to perform the functions described in part (B). • C.) The structure of the cell membrane allows it to perform its functions. The lipid bilayer forms a strong flexible barrier between the cell and its surroundings which repels water and lar ...
... C. Explain how the structure of the cell membrane allows it to perform the functions described in part (B). • C.) The structure of the cell membrane allows it to perform its functions. The lipid bilayer forms a strong flexible barrier between the cell and its surroundings which repels water and lar ...
Plant stem cells: divergent pathways and common themes in shoots
... REPRESSOR OF GAI RNAi RNA interference SCR SCARECROW SHR SHORTROOT STM SHOOT MERISTEMLESS WUS WUSCHEL ...
... REPRESSOR OF GAI RNAi RNA interference SCR SCARECROW SHR SHORTROOT STM SHOOT MERISTEMLESS WUS WUSCHEL ...
Evidence for Gal3p`s Cytoplasmic Location
... the native GAL80 promoter on a single-copy plasmid (pGP15) were grown in medium containing glycerol–lactic acid to early log phase and then induced with galactose for 3 h. Direct fluorescence microscopy showed Gal80GFP as a diffuse signal throughout the cell, with the nuclear signal (Fig. 3A) being ...
... the native GAL80 promoter on a single-copy plasmid (pGP15) were grown in medium containing glycerol–lactic acid to early log phase and then induced with galactose for 3 h. Direct fluorescence microscopy showed Gal80GFP as a diffuse signal throughout the cell, with the nuclear signal (Fig. 3A) being ...
Allogeneic bilayered tissue-engineered skin
... allograft is readily available, concerns regarding the graft rejection and pathogen transmission render it less popular ...
... allograft is readily available, concerns regarding the graft rejection and pathogen transmission render it less popular ...
The neural tube origin of ventral root sheath cells in
... The host hens' eggs were prepared as follows: a window was cut with a scalpel blade and the embryo floated up to the level of the shell by adding calcium- and magnesiumfree Tyrode's solution (CMF), to which had been added a solution of lOOOOi.u. ml"1 penicillin and 1 mgmT 1 Streptomycin in 0-9% sali ...
... The host hens' eggs were prepared as follows: a window was cut with a scalpel blade and the embryo floated up to the level of the shell by adding calcium- and magnesiumfree Tyrode's solution (CMF), to which had been added a solution of lOOOOi.u. ml"1 penicillin and 1 mgmT 1 Streptomycin in 0-9% sali ...
The Role of Exopolysaccharides in Adhesion of
... pelleted by centrifugation at 30000g for 20 min, washed and resuspended in YE medium. After overnight incubation, bacteria were serially diluted and plated onto solid medium. Mutants were selected on the basis of their non-mucoid colonies. One such mutant, a non-capsulate variant (S61NM), was used s ...
... pelleted by centrifugation at 30000g for 20 min, washed and resuspended in YE medium. After overnight incubation, bacteria were serially diluted and plated onto solid medium. Mutants were selected on the basis of their non-mucoid colonies. One such mutant, a non-capsulate variant (S61NM), was used s ...
Adaptively Sampled Distance Fields
... • A cell becomes a candidate for further subdivision when all of the following are true: • it is a leaf cell of level L, or 2L, or 3L, etc. • it can not be trivially determined to be an interior or exterior cell • it does not satisfy a specified error criterion • its level is below a specified maxim ...
... • A cell becomes a candidate for further subdivision when all of the following are true: • it is a leaf cell of level L, or 2L, or 3L, etc. • it can not be trivially determined to be an interior or exterior cell • it does not satisfy a specified error criterion • its level is below a specified maxim ...
Interaction of ZPR1 with translation elongation factor
... CA). Positive clones were examined by automated sequencing (model 373A; Applied Biosystems, Foster City, CA). The S. pombe zpr11 gene was isolated using a similar procedure (Galcheva-Gargova et al., 1998). Subsequently, the open reading frame encoding S. cerevisiae ZPR1 (YGR211w) was identified in t ...
... CA). Positive clones were examined by automated sequencing (model 373A; Applied Biosystems, Foster City, CA). The S. pombe zpr11 gene was isolated using a similar procedure (Galcheva-Gargova et al., 1998). Subsequently, the open reading frame encoding S. cerevisiae ZPR1 (YGR211w) was identified in t ...
Modification of Newcastle Disease Virus Release and
... and fragmentation of cell nuclei. This c.p.e, was also found in lectin-treated cells infected with the avirulent NDV strains F and Queensland which normally induce only limited cell damage. The~ possible mechanisms underlying the varied effects of different lectins on N D V release and cytopathogeni ...
... and fragmentation of cell nuclei. This c.p.e, was also found in lectin-treated cells infected with the avirulent NDV strains F and Queensland which normally induce only limited cell damage. The~ possible mechanisms underlying the varied effects of different lectins on N D V release and cytopathogeni ...
Tissue engineering
Tissue engineering is the use of a combination of cells, engineering and materials methods, and suitable biochemical and physicochemical factors to improve or replace biological functions. While it was once categorized as a sub-field of biomaterials, having grown in scope and importance it can be considered as a field in its own right.While most definitions of tissue engineering cover a broad range of applications, in practice the term is closely associated with applications that repair or replace portions of or whole tissues (i.e., bone, cartilage, blood vessels, bladder, skin, muscle etc.). Often, the tissues involved require certain mechanical and structural properties for proper functioning. The term has also been applied to efforts to perform specific biochemical functions using cells within an artificially-created support system (e.g. an artificial pancreas, or a bio artificial liver). The term regenerative medicine is often used synonymously with tissue engineering, although those involved in regenerative medicine place more emphasis on the use of stem cells or progenitor cells to produce tissues.