CRISPR/Cas9 Screens Reveal Requirements for Host Cell Sulfation
... host processes, often redundantly (Shames and Finlay, 2012). The Gram-negative marine bacterium Vibrio parahaemolyticus is a leading cause worldwide of gastroenteritis linked to the consumption of contaminated seafood and is a cause of major economic losses for the aquaculture industry (reviewed in ...
... host processes, often redundantly (Shames and Finlay, 2012). The Gram-negative marine bacterium Vibrio parahaemolyticus is a leading cause worldwide of gastroenteritis linked to the consumption of contaminated seafood and is a cause of major economic losses for the aquaculture industry (reviewed in ...
The cell biology of neural stem and progenitor cells - MPI
... of neural progenitors during adult neurogenesis in the dentate gyrus of the hippocampus [35] and during neurogenesis in the developing cerebellum [36]. Extrapolating from these observations to embryonic neurogenesis in the neocortex, the intriguing possibility arises that the expansion of APs also i ...
... of neural progenitors during adult neurogenesis in the dentate gyrus of the hippocampus [35] and during neurogenesis in the developing cerebellum [36]. Extrapolating from these observations to embryonic neurogenesis in the neocortex, the intriguing possibility arises that the expansion of APs also i ...
The effect of temperature shifts on protein synthesis
... psychrophiles, psychrotrophs and mesophiles (Ratkowsky et al., 1982, 1983; Reichardt & Morita, 1982). Given the myriad of chemical reactions involved in growth, it would seem likely that the relationship between temperature and metabolic control must be quite complex. It is unclear how cells regulat ...
... psychrophiles, psychrotrophs and mesophiles (Ratkowsky et al., 1982, 1983; Reichardt & Morita, 1982). Given the myriad of chemical reactions involved in growth, it would seem likely that the relationship between temperature and metabolic control must be quite complex. It is unclear how cells regulat ...
Secondary Antibody Responses Immune Complex
... population. One possible explanation for this population is that some or all of these non-germinal center-derived cells are naive B cells that have been activated only upon secondary Ag exposure. If this were true (i.e., that a large portion of secondary Ab responses come from naive cells), it would ...
... population. One possible explanation for this population is that some or all of these non-germinal center-derived cells are naive B cells that have been activated only upon secondary Ag exposure. If this were true (i.e., that a large portion of secondary Ab responses come from naive cells), it would ...
Cell Cycle-Dependent Targeting of a Kinesin at the Plasma
... the KDZ. An example of such an event is shown in Figure 1A. At time point 135 min into mitosis, the cell plate matched with the KDZ at one side of the cell but not with the opposite side. In the following recordings, the entire phragmoplast and cell plate had moved slightly so that now both of the l ...
... the KDZ. An example of such an event is shown in Figure 1A. At time point 135 min into mitosis, the cell plate matched with the KDZ at one side of the cell but not with the opposite side. In the following recordings, the entire phragmoplast and cell plate had moved slightly so that now both of the l ...
changes in the ultrastructure of capsicum annuum l. seedlings roots
... more numerous, vacuoles containing electron-opaque formations (Figs 7, 8), similar to those described in the vacuoles of the cap. The lobate cell nuclei had irregular, folded contours with deep invaginations (Fig. 8), and at some place a partial degradation of the nucleus membrane occurred (Fig. 9). ...
... more numerous, vacuoles containing electron-opaque formations (Figs 7, 8), similar to those described in the vacuoles of the cap. The lobate cell nuclei had irregular, folded contours with deep invaginations (Fig. 8), and at some place a partial degradation of the nucleus membrane occurred (Fig. 9). ...
Cell wall arabinan is essential for guard cell function
... of two monoclonal antibodies, LM5 and LM6. These antibodies were raised against short-chain linear oligomers of (1–4)--Dgalactan (17) and (1–5)-␣-L-arabinan (18), respectively, and have been shown to bind specifically to linear stretches of three or more linked residues but do not recognize single ...
... of two monoclonal antibodies, LM5 and LM6. These antibodies were raised against short-chain linear oligomers of (1–4)--Dgalactan (17) and (1–5)-␣-L-arabinan (18), respectively, and have been shown to bind specifically to linear stretches of three or more linked residues but do not recognize single ...
2.5S NERVE GROWTH FACTOR (NGF) (non
... Cedarlane® purified NGF is prepared from male mouse submandibular gland, and is useful for the study of neuronal growth and differentiation in culture and in vivo. In the peripheral nervous system, Nerve Growth Factor (NGF) promotes the survival and growth of sympathetic and spinal sensory neurons1. ...
... Cedarlane® purified NGF is prepared from male mouse submandibular gland, and is useful for the study of neuronal growth and differentiation in culture and in vivo. In the peripheral nervous system, Nerve Growth Factor (NGF) promotes the survival and growth of sympathetic and spinal sensory neurons1. ...
1 Breast Cancer Cell Line Development and Authentication
... the establishment of cell repositories to house and distribute cells. It also led to the development of tumor specific cancer cell lines that created models of different types of human cancer and to an explosion of understanding of how cells work without the influence or perturbation of other cells. ...
... the establishment of cell repositories to house and distribute cells. It also led to the development of tumor specific cancer cell lines that created models of different types of human cancer and to an explosion of understanding of how cells work without the influence or perturbation of other cells. ...
Herpesvirus Glycoproteins Undergo Multiple Antigenic Changes
... were possibly distinct populations at different stages of infection. To compare them more directly we used mAb SC-9A5, which like BN-1A7 recognizes gB only before capsid release [29] but is IgG3 and so allows isotype-specific co-staining with MG-1A12 (IgG2a). After 1h at 37uC. SC-9A5+ and MG-1A12+ v ...
... were possibly distinct populations at different stages of infection. To compare them more directly we used mAb SC-9A5, which like BN-1A7 recognizes gB only before capsid release [29] but is IgG3 and so allows isotype-specific co-staining with MG-1A12 (IgG2a). After 1h at 37uC. SC-9A5+ and MG-1A12+ v ...
Human Body II
... is real evidence that in the next decades the way that medicine will treat diseases requiring the replacement of organs or tissues will also change. The tissue created in the laboratory will be genetically identical to the patient's, so there will be no rejection. Once this is achieved, transplants ...
... is real evidence that in the next decades the way that medicine will treat diseases requiring the replacement of organs or tissues will also change. The tissue created in the laboratory will be genetically identical to the patient's, so there will be no rejection. Once this is achieved, transplants ...
Tissue Engineered Human Skin Equivalents
... cell proliferation and differentiation is through in vitro cell culture bioreactors that simulate biochemical and mechanical signals and regulate tissue development [14]. Bioreactors have three major components: metabolically active cells that express their differentiated phenotype and produce ECM; ...
... cell proliferation and differentiation is through in vitro cell culture bioreactors that simulate biochemical and mechanical signals and regulate tissue development [14]. Bioreactors have three major components: metabolically active cells that express their differentiated phenotype and produce ECM; ...
A conserved role for kinesin-5 in plant mitosis
... The mitotic spindle separates replicated chromosomes, a hallmark process for the eukaryotic cell. Early observations on the mitotic spindle revealed that the spindle is reliably a bipolar structure in all eukaryotes and differs among taxa primarily in the nature of the poles. In particular, most ani ...
... The mitotic spindle separates replicated chromosomes, a hallmark process for the eukaryotic cell. Early observations on the mitotic spindle revealed that the spindle is reliably a bipolar structure in all eukaryotes and differs among taxa primarily in the nature of the poles. In particular, most ani ...
A conserved role for kinesin-5 in plant mitosis
... The mitotic spindle separates replicated chromosomes, a hallmark process for the eukaryotic cell. Early observations on the mitotic spindle revealed that the spindle is reliably a bipolar structure in all eukaryotes and differs among taxa primarily in the nature of the poles. In particular, most ani ...
... The mitotic spindle separates replicated chromosomes, a hallmark process for the eukaryotic cell. Early observations on the mitotic spindle revealed that the spindle is reliably a bipolar structure in all eukaryotes and differs among taxa primarily in the nature of the poles. In particular, most ani ...
Biological effects of 6 mT static magnetic fields: A comparative study
... exposure to static MF at flux densities below 2 T were not unequivocally found to have adverse health consequences. However, at the current state of knowledge, the biological effects, both in vivo and in vitro, connected to MFs are not univocally interpreted. A deeper knowledge of the negative and/o ...
... exposure to static MF at flux densities below 2 T were not unequivocally found to have adverse health consequences. However, at the current state of knowledge, the biological effects, both in vivo and in vitro, connected to MFs are not univocally interpreted. A deeper knowledge of the negative and/o ...
T cell responses to Chlamydia trachomatis
... with the IFN-γ-mediated increase in MHC expression. Zhong et al. [28] have identified a protease-like factor that is expressed by Chlamydia and secreted into the host cell cytosol. This factor has been shown to be both necessary and sufficient for the degradation of host transcription factors requir ...
... with the IFN-γ-mediated increase in MHC expression. Zhong et al. [28] have identified a protease-like factor that is expressed by Chlamydia and secreted into the host cell cytosol. This factor has been shown to be both necessary and sufficient for the degradation of host transcription factors requir ...
Isolation of Chinese Hamster Ovary Cell Mutants Requiring the
... was able later to isolate mutants with altered colcemid-binding affinity (14, 17). A similar approach has been used in our own laboratory to isolate CHO mutants with clearly defined alterations in a- and ~-tubulin (6-8). Over the last few years, Horwitz and her co-workers (24, 25) have shown that ta ...
... was able later to isolate mutants with altered colcemid-binding affinity (14, 17). A similar approach has been used in our own laboratory to isolate CHO mutants with clearly defined alterations in a- and ~-tubulin (6-8). Over the last few years, Horwitz and her co-workers (24, 25) have shown that ta ...
Intracellular adhesion molecule-1 modulates β-chemokines
... costimulation. A similar set of molecules with perhaps overlapping functions are the LFA-3/CD2 cell-surface antigens. LFA-3 is expressed on the surface of macrophages and dendritic cells and could complement T-cell costimulation through linking the T cell to the APC. To date, however, no direct effe ...
... costimulation. A similar set of molecules with perhaps overlapping functions are the LFA-3/CD2 cell-surface antigens. LFA-3 is expressed on the surface of macrophages and dendritic cells and could complement T-cell costimulation through linking the T cell to the APC. To date, however, no direct effe ...
hnpcc recommendations B.pub - Association for Clinical Genetic
... variation in fixation of the epitope or effects of epitope retrieval, which are not biological phenomena. Geneticists wish to know if there is an abnormal expression pattern that reflects genetic abnormalities, and this requires identification of a well fixed part of the tumour where the intensity o ...
... variation in fixation of the epitope or effects of epitope retrieval, which are not biological phenomena. Geneticists wish to know if there is an abnormal expression pattern that reflects genetic abnormalities, and this requires identification of a well fixed part of the tumour where the intensity o ...
1. INTRODUCTION The skin is the largest organ in the human body
... The design of a wound dressing depends largely on appropriate selection of material. A number of materials have been experimentally and /or clinically studied. Irrespective of the type of material used, it must possess the following characteristics, (Hutmacher 2001) ...
... The design of a wound dressing depends largely on appropriate selection of material. A number of materials have been experimentally and /or clinically studied. Irrespective of the type of material used, it must possess the following characteristics, (Hutmacher 2001) ...
Lecture 6 Notes CH.6
... The Endomembrane System is defined by the interconnections and sharing of membrane between the system components ...
... The Endomembrane System is defined by the interconnections and sharing of membrane between the system components ...
Somite-derived cells replace ventral aortic
... embryonic ECs originate from two different mesodermal lineages (Pardanaud et al., 1996). One, from the splanchnic mesoderm, gives rise to the endothelial network of the visceral organs. This EC production arises in situ (Pardanaud et al., 1989) and exhibits a dual hemangiopoietic potential, i.e. it ...
... embryonic ECs originate from two different mesodermal lineages (Pardanaud et al., 1996). One, from the splanchnic mesoderm, gives rise to the endothelial network of the visceral organs. This EC production arises in situ (Pardanaud et al., 1989) and exhibits a dual hemangiopoietic potential, i.e. it ...
K-Ras is essential for normal fetal liver erythropoiesis
... 10 000 c-kit⫹ fetal liver cells were plated in 96-well plates in serum-free medium (X-VIVO 10; CAMBREX, Walkersville, MD) in the presence of varying concentrations of either EPO or KitL alone or in combination. After 48 hours in culture, cells were pulsed with tritiated thymidine (New Life Sciences, ...
... 10 000 c-kit⫹ fetal liver cells were plated in 96-well plates in serum-free medium (X-VIVO 10; CAMBREX, Walkersville, MD) in the presence of varying concentrations of either EPO or KitL alone or in combination. After 48 hours in culture, cells were pulsed with tritiated thymidine (New Life Sciences, ...
Functions of LIM proteins in cell polarity and chemotactic motility
... of the actin cytoskeleton (Hacker et al., 1997). We studied the dynamics of GFP±LimC and GFP±LimD distribution in Dictyostelium cells during uptake of TRITC±dextran. At the beginning of the sequence shown in Figure 4A, a GFP±LimC-rich membrane invaginates (0 s). The protrusion of the GFP±LimC-rich m ...
... of the actin cytoskeleton (Hacker et al., 1997). We studied the dynamics of GFP±LimC and GFP±LimD distribution in Dictyostelium cells during uptake of TRITC±dextran. At the beginning of the sequence shown in Figure 4A, a GFP±LimC-rich membrane invaginates (0 s). The protrusion of the GFP±LimC-rich m ...
INTERLEUKIN 6 DECREASES CELL
... and plasmacytomas (14-16), and EBVinfected human B cells (17), but it inhibits the proliferation of a number of breast carcinoma cell lines and of myelomonocytic Ml cells (11, 18, 19). Epithelial cells are connected to each other through a complex system ofjunctions (20-23). An important question co ...
... and plasmacytomas (14-16), and EBVinfected human B cells (17), but it inhibits the proliferation of a number of breast carcinoma cell lines and of myelomonocytic Ml cells (11, 18, 19). Epithelial cells are connected to each other through a complex system ofjunctions (20-23). An important question co ...
Tissue engineering
Tissue engineering is the use of a combination of cells, engineering and materials methods, and suitable biochemical and physicochemical factors to improve or replace biological functions. While it was once categorized as a sub-field of biomaterials, having grown in scope and importance it can be considered as a field in its own right.While most definitions of tissue engineering cover a broad range of applications, in practice the term is closely associated with applications that repair or replace portions of or whole tissues (i.e., bone, cartilage, blood vessels, bladder, skin, muscle etc.). Often, the tissues involved require certain mechanical and structural properties for proper functioning. The term has also been applied to efforts to perform specific biochemical functions using cells within an artificially-created support system (e.g. an artificial pancreas, or a bio artificial liver). The term regenerative medicine is often used synonymously with tissue engineering, although those involved in regenerative medicine place more emphasis on the use of stem cells or progenitor cells to produce tissues.