Genetically Engineered Cell Lines: Characterisation and
... enzymes. Attempts were made to overcome this shortcoming by supplying the cell lines with an exogenous source of these enzymes, such as the post-mitochondrial supernatant of the liver. However, this approach was only partially successful. The exogenous enzyme preparations were difficult to standardi ...
... enzymes. Attempts were made to overcome this shortcoming by supplying the cell lines with an exogenous source of these enzymes, such as the post-mitochondrial supernatant of the liver. However, this approach was only partially successful. The exogenous enzyme preparations were difficult to standardi ...
The mechanism of leaf morphogenesis
... variable. Cells in a meristem are maintained at a particular size, but as cells are removed from the meristem this balance between size and division is shifted. Moreover, this shift occurs in a tissue-specific manner (in fact, in some ways defines tissue identity). The mechanism controlling this shift ...
... variable. Cells in a meristem are maintained at a particular size, but as cells are removed from the meristem this balance between size and division is shifted. Moreover, this shift occurs in a tissue-specific manner (in fact, in some ways defines tissue identity). The mechanism controlling this shift ...
A gain-of-function mutant of Munc18-1 stimulates secretory granule
... addition, Munc18-1 may undergo all three modes of interaction [25,38,39], and modes 2 and 3 are required for Munc18-1 to stimulate membrane fusion kinetics in vitro [25]. Mode 1 binding has only been observed with Munc18-1 and its role in exocytosis has been debated. It has been suggested that this ...
... addition, Munc18-1 may undergo all three modes of interaction [25,38,39], and modes 2 and 3 are required for Munc18-1 to stimulate membrane fusion kinetics in vitro [25]. Mode 1 binding has only been observed with Munc18-1 and its role in exocytosis has been debated. It has been suggested that this ...
INTERLEUKIN 6 DECREASES CELL
... and plasmacytomas (14-16), and EBVinfected human B cells (17), but it inhibits the proliferation of a number of breast carcinoma cell lines and of myelomonocytic Ml cells (11, 18, 19). Epithelial cells are connected to each other through a complex system ofjunctions (20-23). An important question co ...
... and plasmacytomas (14-16), and EBVinfected human B cells (17), but it inhibits the proliferation of a number of breast carcinoma cell lines and of myelomonocytic Ml cells (11, 18, 19). Epithelial cells are connected to each other through a complex system ofjunctions (20-23). An important question co ...
1-1 Test Bank Huether: Understanding Pathophysiology, 4th Edition
... A. Cholesterol decreases the membrane fluidity of the erythrocyte, which reduces its ability to carry oxygen. B. Cholesterol decreases the membrane fluidity of erythrocytes, which reduces its ability to carry hemoglobin. C. Cholesterol increases the membrane fluidity of erythrocytes, which allows bi ...
... A. Cholesterol decreases the membrane fluidity of the erythrocyte, which reduces its ability to carry oxygen. B. Cholesterol decreases the membrane fluidity of erythrocytes, which reduces its ability to carry hemoglobin. C. Cholesterol increases the membrane fluidity of erythrocytes, which allows bi ...
Ophiobolin A from Bipolaris oryzae Perturbs Motility and Membrane
... plasma membrane) was lethal to the sperm cells in concentrations below 2000 ng/mL. Similarly to the effect seen in boar spermatozoa, OPA inhibited the motility and reduced the viability of mouse sperms in the work of Zeng et al., and, besides these effects, the inhibition of sperm capacitation was a ...
... plasma membrane) was lethal to the sperm cells in concentrations below 2000 ng/mL. Similarly to the effect seen in boar spermatozoa, OPA inhibited the motility and reduced the viability of mouse sperms in the work of Zeng et al., and, besides these effects, the inhibition of sperm capacitation was a ...
The evodevo of multinucleate cells, tissues, and organisms, and an
... however Burkholder and McVeigh 1941) and in algal clades as different as the Rhodophyceae (e.g., Griffithsia), Xanthophyceae (e.g., Vaucheria), Chlorophyceae (e.g., Pediastrum), Ulvophyceae (e.g., Cladophora), and Charophyceae (e.g., Nitella) raises a number of important but as yet unresolved questio ...
... however Burkholder and McVeigh 1941) and in algal clades as different as the Rhodophyceae (e.g., Griffithsia), Xanthophyceae (e.g., Vaucheria), Chlorophyceae (e.g., Pediastrum), Ulvophyceae (e.g., Cladophora), and Charophyceae (e.g., Nitella) raises a number of important but as yet unresolved questio ...
Comparison of cytotoxicity and wound healing effect of
... promotes epithelial healing[3,4]. Carboxymethylcellulose (CMC), a high-molecular-weight polysaccharide, is one of the most common viscous polymers used in artificial tears to achieve their prolonged residence efficacious in the treatment of aqueous tear-deficient dry eye symptoms and ocular surface ...
... promotes epithelial healing[3,4]. Carboxymethylcellulose (CMC), a high-molecular-weight polysaccharide, is one of the most common viscous polymers used in artificial tears to achieve their prolonged residence efficacious in the treatment of aqueous tear-deficient dry eye symptoms and ocular surface ...
Mechanisms for interaction between RF fields and biological tissue
... effective over a fairly broad range of frequencies, n, Vibrations by RF Fields close to those given by ot ¼ 1, where o ¼ 2pn and t is A number of interaction mechanisms that have the average time the dipoles take to re-orient. Since been considered are based on the excitation of t ¼ 4 1011 s for ...
... effective over a fairly broad range of frequencies, n, Vibrations by RF Fields close to those given by ot ¼ 1, where o ¼ 2pn and t is A number of interaction mechanisms that have the average time the dipoles take to re-orient. Since been considered are based on the excitation of t ¼ 4 1011 s for ...
VCE_MFST1500-4
... organisms whose growth on foods usually can be recognized. The main part of the growth commonly is white, but it may be colored, dark, or smokey. Colored spores are typical of some types of mature mold growth and may give color to part or all of the growth. Holds normally reproduce by spore formatio ...
... organisms whose growth on foods usually can be recognized. The main part of the growth commonly is white, but it may be colored, dark, or smokey. Colored spores are typical of some types of mature mold growth and may give color to part or all of the growth. Holds normally reproduce by spore formatio ...
mRNA delivery for
... growth [32], and in several cancers where it interferes with the maturation of members of the let7 family of miRNAs [33–35], which regulate Hmga2 and downstream targets such as Snai1 that promote metastasis and invasion [36–39]. We transfected fibroblasts five times at 48-hour intervals with Lin28-e ...
... growth [32], and in several cancers where it interferes with the maturation of members of the let7 family of miRNAs [33–35], which regulate Hmga2 and downstream targets such as Snai1 that promote metastasis and invasion [36–39]. We transfected fibroblasts five times at 48-hour intervals with Lin28-e ...
Fermentation of Tomato Juice by Cell Immobilized Lactobacillus
... was found that tomato juice was a proper medium for lactic acid fermentation, and the probiotic tomato juice obtained could serve as a health beverage for vegetarians or consumers who are allergic to dairy products. Immobilized L. acidophilus has been used in banana puree fermentation [13,14], and i ...
... was found that tomato juice was a proper medium for lactic acid fermentation, and the probiotic tomato juice obtained could serve as a health beverage for vegetarians or consumers who are allergic to dairy products. Immobilized L. acidophilus has been used in banana puree fermentation [13,14], and i ...
yellowish discloration to the tissues of the body (jaundice or icterus)
... “Bilirubin” is a normal bile pigment formed from the breakdown of hemoglobin; “hemoglobin” is the compound in the redblood cells that carries oxygen to the tissues of the body; the liver takes up the hemoglobin following normal or abnormal breakdown of red-blood cells and processes it to form bile ...
... “Bilirubin” is a normal bile pigment formed from the breakdown of hemoglobin; “hemoglobin” is the compound in the redblood cells that carries oxygen to the tissues of the body; the liver takes up the hemoglobin following normal or abnormal breakdown of red-blood cells and processes it to form bile ...
Demonstration by single-cell PCR that Reed–Sternberg cells and
... the standard B95.8 genome (Table 2). Interestingly, these point mutations in the LMP-1 gene have already been described in HD and nasopharyngeal carcinoma (Knecht et al., 1993). As suggested in a previous report (Meggetto et al., 1997), the results of the present study further support the suggestion ...
... the standard B95.8 genome (Table 2). Interestingly, these point mutations in the LMP-1 gene have already been described in HD and nasopharyngeal carcinoma (Knecht et al., 1993). As suggested in a previous report (Meggetto et al., 1997), the results of the present study further support the suggestion ...
the Text
... overall growth. The main differences between greenhouse and outdoor conditions are higher fluence (more photons) and the presence UV-A and UV-B photons outdoors. Depending on ambient weather conditions outdoor plants might also experience cooler nights or warmer days than the greenhouse plants. Cove ...
... overall growth. The main differences between greenhouse and outdoor conditions are higher fluence (more photons) and the presence UV-A and UV-B photons outdoors. Depending on ambient weather conditions outdoor plants might also experience cooler nights or warmer days than the greenhouse plants. Cove ...
yellowish discloration to the tissues of the body (jaundice or icterus)
... “Bilirubin” is a normal bile pigment formed from the breakdown of hemoglobin; “hemoglobin” is the compound in the redblood cells that carries oxygen to the tissues of the body; the liver takes up the hemoglobin following normal or abnormal breakdown of red-blood cells and processes it to form bile ...
... “Bilirubin” is a normal bile pigment formed from the breakdown of hemoglobin; “hemoglobin” is the compound in the redblood cells that carries oxygen to the tissues of the body; the liver takes up the hemoglobin following normal or abnormal breakdown of red-blood cells and processes it to form bile ...
Abscisic acid
... impaired ABA response and reduced drought resistance. They found two copies of PYR1 fit snugly together in plant cells, and they are receptors for ABA. Each copy of the PYR1 molecule has an internal open space like the inside of a tin can, and when a ABA molecule comes along, it fits into one of the ...
... impaired ABA response and reduced drought resistance. They found two copies of PYR1 fit snugly together in plant cells, and they are receptors for ABA. Each copy of the PYR1 molecule has an internal open space like the inside of a tin can, and when a ABA molecule comes along, it fits into one of the ...
The role of Pex3p in early events of peroxisome biogenesis in
... rescue of peroxisomes in H. polymorpha pex3 cells. pex3 cells lack morphologically detectable peroxisomal membrane remnants ("ghosts") and thus, hypothetically, a peroxisomal membrane template for peroxisome re-assembly. However, reintroduction of WT Pex3p in the mutant led to the rapid reappearance ...
... rescue of peroxisomes in H. polymorpha pex3 cells. pex3 cells lack morphologically detectable peroxisomal membrane remnants ("ghosts") and thus, hypothetically, a peroxisomal membrane template for peroxisome re-assembly. However, reintroduction of WT Pex3p in the mutant led to the rapid reappearance ...
Expression of collagenolytic/gelatinolytic metalloproteinases
... culture were performed essentially as described.23 Epithelial cells and stromal fibroblasts were plated at desired densities in 16-mm diameter wells of 24-well cluster dishes. Cell adherence and spreading was facilitated by inclusion of 10% calf scrum (Hyclone, Logan, UT) in the medium, but after 16 ...
... culture were performed essentially as described.23 Epithelial cells and stromal fibroblasts were plated at desired densities in 16-mm diameter wells of 24-well cluster dishes. Cell adherence and spreading was facilitated by inclusion of 10% calf scrum (Hyclone, Logan, UT) in the medium, but after 16 ...
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... can be obtained without expertise in image processing. Second, the verification of previously, manually or automatically acquired results is possible. Third, the summary information produced allows for manual discarding of unreliable results. Fourth, in addition to basic enumeration of objects, the ...
... can be obtained without expertise in image processing. Second, the verification of previously, manually or automatically acquired results is possible. Third, the summary information produced allows for manual discarding of unreliable results. Fourth, in addition to basic enumeration of objects, the ...
Cellular Structure and Function
... under a microscope when he happened to see them dividing and forming new cells. He realized that living cells produce new cells through division. Based on this realization, Virchow proposed that living cells arise only from other living cells. The ideas of all three scientists—Schwann, Schleiden, an ...
... under a microscope when he happened to see them dividing and forming new cells. He realized that living cells produce new cells through division. Based on this realization, Virchow proposed that living cells arise only from other living cells. The ideas of all three scientists—Schwann, Schleiden, an ...
Cell Cycle-specific Effects of Tumor Necrosis
... acid buffer (Calbiochem. La Jolla. Calif.) and 2 mm MgCI2, pH 6.4 and 0°.DAPI was synthesized and kindly provided by Dr. Jan Kapuscinski of the Sloan-Kettering Institute. At 0.1% Triton X-100 and in the presence of serum proteins (from the original suspen sion), the cells do not lyse (unless subjec ...
... acid buffer (Calbiochem. La Jolla. Calif.) and 2 mm MgCI2, pH 6.4 and 0°.DAPI was synthesized and kindly provided by Dr. Jan Kapuscinski of the Sloan-Kettering Institute. At 0.1% Triton X-100 and in the presence of serum proteins (from the original suspen sion), the cells do not lyse (unless subjec ...
HISTOLOGICAL AND SEM STUDIES ON ORGANOGENESIS IN
... (Góralski et al., 2005) still displayed callus proliferation and shoot regeneration potential after being maintained more than 2 years. The differences in regeneration efficiency (number of shoots per explant) between our previous studies (Góralski et al., 2005) and the new culture were not statisti ...
... (Góralski et al., 2005) still displayed callus proliferation and shoot regeneration potential after being maintained more than 2 years. The differences in regeneration efficiency (number of shoots per explant) between our previous studies (Góralski et al., 2005) and the new culture were not statisti ...
TCR After Rapid Identification of Pathogenic Vaccines Autoimmune
... Based on findings obtained by CDR3 spectratyping, bands representing candidate spectratypes were cut out from the gel and extracted cDNA was reamplified by nested PCR. PCR products were then cloned and the nucleotide sequences of the clones were determined. The results are listed in Tables 1 and 2. ...
... Based on findings obtained by CDR3 spectratyping, bands representing candidate spectratypes were cut out from the gel and extracted cDNA was reamplified by nested PCR. PCR products were then cloned and the nucleotide sequences of the clones were determined. The results are listed in Tables 1 and 2. ...
Carbohydrates Chapter 16
... - The glycosaminoglycan hyaluronic acid contains alternating residues of D-glucuronic acid and N-acetylglucosamine Forming clear, highly viscous solutions that serve as lubricants in the synovial fluid of joints and giving the vitreous humor of the eye its jelly-like consistency Essential compon ...
... - The glycosaminoglycan hyaluronic acid contains alternating residues of D-glucuronic acid and N-acetylglucosamine Forming clear, highly viscous solutions that serve as lubricants in the synovial fluid of joints and giving the vitreous humor of the eye its jelly-like consistency Essential compon ...
Tissue engineering
Tissue engineering is the use of a combination of cells, engineering and materials methods, and suitable biochemical and physicochemical factors to improve or replace biological functions. While it was once categorized as a sub-field of biomaterials, having grown in scope and importance it can be considered as a field in its own right.While most definitions of tissue engineering cover a broad range of applications, in practice the term is closely associated with applications that repair or replace portions of or whole tissues (i.e., bone, cartilage, blood vessels, bladder, skin, muscle etc.). Often, the tissues involved require certain mechanical and structural properties for proper functioning. The term has also been applied to efforts to perform specific biochemical functions using cells within an artificially-created support system (e.g. an artificial pancreas, or a bio artificial liver). The term regenerative medicine is often used synonymously with tissue engineering, although those involved in regenerative medicine place more emphasis on the use of stem cells or progenitor cells to produce tissues.