The World of Cells Kinds of Cells Tour of a Eukaryotic Cell Transport
... ones, are not powerful enough to resolve many structures within cells. For example, a membrane is only 5 nanometers thick. Why not just add another magnifying stage to the microscope and so increase its resolving power? Because when two objects are closer than a few hundred nanometers, the light bea ...
... ones, are not powerful enough to resolve many structures within cells. For example, a membrane is only 5 nanometers thick. Why not just add another magnifying stage to the microscope and so increase its resolving power? Because when two objects are closer than a few hundred nanometers, the light bea ...
The pollen wall and tapetum are altered in the
... microsporocyte meiosis and primexine deposition appeared to occur normally in the RC7 line. At the tetrad stage, primexine appeared to be deposited on the microspores based on the regular undulation of the plasma membrane. Primexine with the callose wall may serve as a molecular filter, allowing the ...
... microsporocyte meiosis and primexine deposition appeared to occur normally in the RC7 line. At the tetrad stage, primexine appeared to be deposited on the microspores based on the regular undulation of the plasma membrane. Primexine with the callose wall may serve as a molecular filter, allowing the ...
Lamin proteins form an internal nucleoskeleton as well as a
... Fig. 1. Mapping the epitope recognized by monoclonal antibody 133A2. (A) Schematic diagrams of lamin A and C (which is identical to lamin A except for 6 amino acids at the C terminus) and of lamin A deletion mutants. The diagrams are not drawn to scale; central regions have been omitted (indicated b ...
... Fig. 1. Mapping the epitope recognized by monoclonal antibody 133A2. (A) Schematic diagrams of lamin A and C (which is identical to lamin A except for 6 amino acids at the C terminus) and of lamin A deletion mutants. The diagrams are not drawn to scale; central regions have been omitted (indicated b ...
Promega Notes 99: NIH Chemical Genomics Center: Small
... The P450 gene superfamily is involved in the metabolism and clearance of xenobiotics. For example, the NCGC used human CYP2C9 to measure the hydroxylation of deoxyluciferin (Luciferin-H; P450-Glo™ CYP2C9 Assay; Cat.# V8791) to luciferin. Luciferin was measured by luminescence after adding the lucife ...
... The P450 gene superfamily is involved in the metabolism and clearance of xenobiotics. For example, the NCGC used human CYP2C9 to measure the hydroxylation of deoxyluciferin (Luciferin-H; P450-Glo™ CYP2C9 Assay; Cat.# V8791) to luciferin. Luciferin was measured by luminescence after adding the lucife ...
Pulsatile Stretch Remodels Cell-to-Cell Communication in Cultured
... junction protein connexin43 (Cx43) and the fascia adherens junction protein N-cadherin was measured immunohistochemically in the same preparations. Pulsatile stretch caused dramatic upregulation of intercellular junction proteins after only 1 hour and a further increase after 6 hours (Cx43 signal in ...
... junction protein connexin43 (Cx43) and the fascia adherens junction protein N-cadherin was measured immunohistochemically in the same preparations. Pulsatile stretch caused dramatic upregulation of intercellular junction proteins after only 1 hour and a further increase after 6 hours (Cx43 signal in ...
Control of cell cycle transcription during G1 and S phases
... are highly conserved7–9,10. Intriguingly, recent work demonstrated that DNA checkpoint control depends on the same transcription factors responsible for commitment point regulation11. The dynamic changes in gene expression as a function of cell cycle progression are regulated by specific CDK activi ...
... are highly conserved7–9,10. Intriguingly, recent work demonstrated that DNA checkpoint control depends on the same transcription factors responsible for commitment point regulation11. The dynamic changes in gene expression as a function of cell cycle progression are regulated by specific CDK activi ...
The Plasma Membrane
... some small molecules from moving through the membrane. • They keep the membrane at an optimal level of fluidity. ...
... some small molecules from moving through the membrane. • They keep the membrane at an optimal level of fluidity. ...
Development
... is transiently induced in soybean roots by external treatment of the plants with chitin pentaose (Minami et al., 1996). These data support a hypothesis that (lipo)chitin oligosaccharides have mitogenic activity in various organisms. We were interested to know whether an intact LCO molecule is a prer ...
... is transiently induced in soybean roots by external treatment of the plants with chitin pentaose (Minami et al., 1996). These data support a hypothesis that (lipo)chitin oligosaccharides have mitogenic activity in various organisms. We were interested to know whether an intact LCO molecule is a prer ...
Reprogramming of gene expression following nuclear transfer to the
... molecular marker can be measured obscured the analysis of such reprogramming experiments. To overcome these difficulties, we have performed nuclear transfers using the immediate precursor of the egg, the oocyte. The egg is blocked in metaphase II of meiosis and resumes development as soon as a nucleus ...
... molecular marker can be measured obscured the analysis of such reprogramming experiments. To overcome these difficulties, we have performed nuclear transfers using the immediate precursor of the egg, the oocyte. The egg is blocked in metaphase II of meiosis and resumes development as soon as a nucleus ...
Recent Advances in αβ T Cell Biology: Wnt Signaling
... differentiation of functional IFN-γ-secreting Th1 cells during infection with L. major [49]. Differentiation of other Th cells is also influenced by Notch signaling. It is shown that Notch binds to the promoter of GATA3, a master regulator of Th2 differentiation, thus inducing the expression of the ...
... differentiation of functional IFN-γ-secreting Th1 cells during infection with L. major [49]. Differentiation of other Th cells is also influenced by Notch signaling. It is shown that Notch binds to the promoter of GATA3, a master regulator of Th2 differentiation, thus inducing the expression of the ...
Functional characterization of cohesin subunit SCC1 in
... SCC1 orthologue. TbSCC1 is expressed prior to DNA synthesis at late G1, remains in the nucleus throughout S- and G2-phases of the cell cycle and disappears at anaphase. Depletion of SCC1 by RNAi or expression of a non-cleavable SCC1 resulted in karyokinesis failure. Using the dominant negative pheno ...
... SCC1 orthologue. TbSCC1 is expressed prior to DNA synthesis at late G1, remains in the nucleus throughout S- and G2-phases of the cell cycle and disappears at anaphase. Depletion of SCC1 by RNAi or expression of a non-cleavable SCC1 resulted in karyokinesis failure. Using the dominant negative pheno ...
The Aromatic-L-Amino Acid Decarboxylase Inhibitor Carbidopa Is
... Arlington Heights, IL) with the Rediprime DNA Labeling System (Amersham) and purified on a NENSORB 20 cartridge. Posthybridization washes were: 5⫻ SSC (twice for 2 min at 24°C); 2⫻ SSC, 0.1% SDS (four times for 5 min at 24°C); and 0.1⫻ SSC, 0.1% SDS (three times for 15 min at 50°C). Autoradiography ...
... Arlington Heights, IL) with the Rediprime DNA Labeling System (Amersham) and purified on a NENSORB 20 cartridge. Posthybridization washes were: 5⫻ SSC (twice for 2 min at 24°C); 2⫻ SSC, 0.1% SDS (four times for 5 min at 24°C); and 0.1⫻ SSC, 0.1% SDS (three times for 15 min at 50°C). Autoradiography ...
PDF
... one would expect shifting of cell genesis order within a phase (A-B or B-A, or C-D-E or E-C-D, etc.), but not between phases (never AC-B, C-B-E, etc.). Finally, the most extreme form of competence control is where RPCs pass sequentially through periods to make each cell type separately. In our examp ...
... one would expect shifting of cell genesis order within a phase (A-B or B-A, or C-D-E or E-C-D, etc.), but not between phases (never AC-B, C-B-E, etc.). Finally, the most extreme form of competence control is where RPCs pass sequentially through periods to make each cell type separately. In our examp ...
Defining retinal progenitor cell competence in
... one would expect shifting of cell genesis order within a phase (A-B or B-A, or C-D-E or E-C-D, etc.), but not between phases (never AC-B, C-B-E, etc.). Finally, the most extreme form of competence control is where RPCs pass sequentially through periods to make each cell type separately. In our examp ...
... one would expect shifting of cell genesis order within a phase (A-B or B-A, or C-D-E or E-C-D, etc.), but not between phases (never AC-B, C-B-E, etc.). Finally, the most extreme form of competence control is where RPCs pass sequentially through periods to make each cell type separately. In our examp ...
Microtubule cortical array organization and plant cell morphogenesis
... their organization. Interphase microtubule cortical arrays assume a variety of configurations that vary by cell type and shape. In cells that are destined to undergo rapid axial elongation, such as those in the root axis or the etiolated hypocotyl, the cortical array assumes a high degree of order, ...
... their organization. Interphase microtubule cortical arrays assume a variety of configurations that vary by cell type and shape. In cells that are destined to undergo rapid axial elongation, such as those in the root axis or the etiolated hypocotyl, the cortical array assumes a high degree of order, ...
Plankton Biol. Ecol. 52(2), Page 67, 2005
... by an order of magnitude (Kobori & Taga 1979; Hernandez ...
... by an order of magnitude (Kobori & Taga 1979; Hernandez ...
Developmental plasticity, cell fate specification and morphogenesis
... and compensate for perturbations in the positions and numbers of cells. Removing blastomeres, rearranging them or making chimaeras of more than one embryo can all result in the formation of a blastocyst, indicating a flexibility in cell potential until the 32-cell stage [4–7]. This ability of cells ...
... and compensate for perturbations in the positions and numbers of cells. Removing blastomeres, rearranging them or making chimaeras of more than one embryo can all result in the formation of a blastocyst, indicating a flexibility in cell potential until the 32-cell stage [4–7]. This ability of cells ...
Rewiring the retinal ganglion cell gene regulatory
... expressed in the developing retina at overlapping times but in largely distinct, interspersed RPC subpopulations (Vetter and Brown, 2001; Akagi et al., 2004; Hatakeyama and Kageyama, 2004; Le et al., 2006; Ohsawa and Kageyama, 2008; Trimarchi et al., 2008). The bHLH factors collaborate with homeobox ...
... expressed in the developing retina at overlapping times but in largely distinct, interspersed RPC subpopulations (Vetter and Brown, 2001; Akagi et al., 2004; Hatakeyama and Kageyama, 2004; Le et al., 2006; Ohsawa and Kageyama, 2008; Trimarchi et al., 2008). The bHLH factors collaborate with homeobox ...
Brassinosteroids Regulate Plasma Membrane Anion Channels in
... BRs. V-ATPases are supposed to translocate osmolytes from the cytosol to the vacuole. Mutation in the DET3 gene encoding a V-ATPase reduced the effects of BR (Schumacher et al. 1999) and it would be interesting to see the impact of BRs on the recently described tonoplast proteomic analysis of A. tha ...
... BRs. V-ATPases are supposed to translocate osmolytes from the cytosol to the vacuole. Mutation in the DET3 gene encoding a V-ATPase reduced the effects of BR (Schumacher et al. 1999) and it would be interesting to see the impact of BRs on the recently described tonoplast proteomic analysis of A. tha ...
Mast cells and dendritic cells form synapses that facilitate antigen
... the control, and negative controls were run with all IHC reactions (Fig. S1). IHC revealed that it is common to find MCs and DCs in close proximity (Fig. 1 A, arrows). To quantify the potential for cell–cell contacts, we determined the percentage of MCs that were immediately adjacent to a DC and fou ...
... the control, and negative controls were run with all IHC reactions (Fig. S1). IHC revealed that it is common to find MCs and DCs in close proximity (Fig. 1 A, arrows). To quantify the potential for cell–cell contacts, we determined the percentage of MCs that were immediately adjacent to a DC and fou ...
Herceptin Resistance Database for Understanding
... against Herceptin, several studies have been carried out taking tissue sample or cell line based model systems17,18. Unfortunately, at present we do not have a single platform, where one can correlate experiments done with Herceptin with all the genomic factors such as mutation, expression and CNV, ...
... against Herceptin, several studies have been carried out taking tissue sample or cell line based model systems17,18. Unfortunately, at present we do not have a single platform, where one can correlate experiments done with Herceptin with all the genomic factors such as mutation, expression and CNV, ...
Biology: Concepts and Connections, 6e (Campbell)
... 12) Diffusion does not require the cell to expend ATP. Therefore, diffusion is considered a type of A) exocytosis. B) phagocytosis. C) passive transport. D) active transport. E) endocytosis. Answer: C 13) Osmosis can be defined as A) the diffusion of water. B) the diffusion of nonpolar molecules. C) ...
... 12) Diffusion does not require the cell to expend ATP. Therefore, diffusion is considered a type of A) exocytosis. B) phagocytosis. C) passive transport. D) active transport. E) endocytosis. Answer: C 13) Osmosis can be defined as A) the diffusion of water. B) the diffusion of nonpolar molecules. C) ...