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Step A
Step A

... Cell Challenge • As a group, quietly discuss each question and agree upon one correct answer. The group with the most correct answers will win extra credit. ...
Imaging the fate of histone Cse4 reveals de novo replacement in S
Imaging the fate of histone Cse4 reveals de novo replacement in S

... Despite this simplicity, the architecture of Cse4 nucleosomes has become the subject of much debate. Cse4 nucleosomes have been reported to differ from the canonical nucleosome not only by the replacement of both molecules of histone H3 by the Cse4 variant, but also by the presence of chaperone Scm3 ...
Cytokinesis in Higher Plants
Cytokinesis in Higher Plants

... in diameter (74). The smaller dark vesicles predominate initially, whereas the larger light vesicles appear more numerous at a later stage of cell plate formation. Both size calculations and the occurrence of hourglass-shaped putative vesicle fusion intermediates suggest that pairwise fusion of smal ...
Endoplasmic reticulum stress-induced programmed cell death in
Endoplasmic reticulum stress-induced programmed cell death in

... expression levels, compared with untreated cells. Fig. 1. Expression of ER proteins in response to CPA treatment. Protein extracts from untreated and CPA-treated soybean cells were analyzed by western blotting using antiBiP (A), anti-CRT (B) and anti-caspase 12 (C) antibodies. Quantitative analyses ...
Tensile Properties of Arabidopsis Cell Walls Depend on Both a
Tensile Properties of Arabidopsis Cell Walls Depend on Both a

... statistical comparisons used the loge transformations. For mur2-1, the modulus was not significantly different from wild type, whereas the difference in strength was significant at the 5% level. Comparing strength and modulus of the other mutants with wild type, differences were highly significant ( ...
Evolutionary aspects of non-cell-autonomous regulation in vascular
Evolutionary aspects of non-cell-autonomous regulation in vascular

... to our knowledge, only one exception, namely, the unique structure of expanded PD in angle and apical meristems of Selaginella willdenovii (Wochok and Clayton, 1976). A prominent feature of PD in angiosperms is their participation in the cell-to-cell spread of viruses. Interestingly, there are very ...
Guidelines for Building Self-Organizing Maps
Guidelines for Building Self-Organizing Maps

... of observations.  Cells adjust their weights with each  placement by becoming more similar to cells in their  immediate vicinity which have had assignments. – Create a list of observations with values on a number of  variables (dimensions) – Compute the similarity among observations based on all  va ...
Inhibition of Cyclin-dependent Kinase Activity Triggers Neuronal
Inhibition of Cyclin-dependent Kinase Activity Triggers Neuronal

Intercourse Between Cell Wall and Cytoplasm Exemplified by
Intercourse Between Cell Wall and Cytoplasm Exemplified by

... however, the discovery in the 1990s that active Yariv disrupts growth has made it an invaluable probe for studying arabinogalactan protein function in vivo. A pioneering demonstration of the biological effect of active Yariv reagent on plant development was the inhibition of proliferation of suspens ...
2, 3, and 4-Electrode Experiments
2, 3, and 4-Electrode Experiments

... roughly—B. Three-electrode setups have a distinct experimental advantage over two electrode setups: they measure only one half of the cell. That is, the potential changes of the working electrode are measured independent of changes that may occur at the counter electrode. This isolation allows for a ...
Cell Structure and Function Chapter 4 Outline Cell Theory Cell Size
Cell Structure and Function Chapter 4 Outline Cell Theory Cell Size

... Cell theory states all organisms are composed of cells and that cells come only from preexisting cells. – One of unifying concepts of biology. ...
Ophiobolin A from Bipolaris oryzae Perturbs Motility and Membrane
Ophiobolin A from Bipolaris oryzae Perturbs Motility and Membrane

... Filamentous fungi can produce large number of secondary metabolites with promising biological effects [1]. Molecular identification of these compounds and characterization of their biological activities are among the most important goals of studies working on the interface of chemistry and biology r ...
Posttranscriptional Gene Silencing-2015
Posttranscriptional Gene Silencing-2015

... The undifferentiated state of malignant cells is correlated with a decrease in miRNA expression c13orf25 miRNA is the first non-coding oncogene, is upregulated by c-Myc, and is involved in leukemia development ...
knockout, knock-in, and conditional mutant mice
knockout, knock-in, and conditional mutant mice

... usually is needed to obtain homologous recombination at practical frequencies. The relationship between sequence divergence and targeting rates has not been examined systematically, but about 0.5% sequence divergence was shown to result in a 20-fold decrease in targeting frequency for constructs tar ...
Truncated products of the vestigial proliferation gene
Truncated products of the vestigial proliferation gene

... of vg in all imaginal discs leads to ectopic proliferation of wing tissue.8 All vg mutants characterized so far have been considered to be hypomorph with a wing phentoype due to cell death, as a consequence of a lack of normal Vg product. Here we propose an alternative hypothesis based on the fact t ...
Quantitative phase microscopy – a new tool for investigating
Quantitative phase microscopy – a new tool for investigating

... over designated periods of time.20 It is important to appreciate that methodologies previously established for the measurement of cell growth in culture are either destructive or extremely laborious. These include cell size measurement with fluorescence activated cell sorting (FACS, which requires r ...
Concentration gradient
Concentration gradient

... – a transport process that uses an energy source produced by the cell for the movement of a polar substance through a membrane – substance(s) moves UP a concentration gradient – uses pumps for primary active transport – uses carriers for secondary active transport ...
Cellular Force Microscopy for in Vivo
Cellular Force Microscopy for in Vivo

... indentation depth. The stiffness obtained reflects not only cell wall elastic properties but also turgor pressure (Smith et al., 1998; Wang et al., 2004), cell and indenter geometry (Bolduc et al., 2006), and mechanical stresses prior to indentation (Zamir and Taber, 2004). For that reason, we will ...
Changes in Cell Morphology and the Cellular Localization of Protein
Changes in Cell Morphology and the Cellular Localization of Protein

Chapter 12 Cytoskeleton
Chapter 12 Cytoskeleton

... cytoplasmic fluid Myosin-binding sites exposed ...
CRISPR/Cas9 Screens Reveal Requirements for Host Cell Sulfation
CRISPR/Cas9 Screens Reveal Requirements for Host Cell Sulfation

MORPHOLOGICAL ALTERATIONS
MORPHOLOGICAL ALTERATIONS

... Describe the nuclear and cytoplasmic features of necrosis. Define and briefly describe the patterns of Tissue Necrosis including: Coagulative Necrosis, Liquefactive Necrosis,Gangrenous Necrosis, Caseous Necrosis, Fat Necrosis and Fibrinoid Necrosis. ...
Intercellular signalling and the multiplication of prokaryotes
Intercellular signalling and the multiplication of prokaryotes

... Tissue cultures of cells taken from higher, differentiated organisms normally need complex (and  mainly polypeptidic or proteinaceous) extracellular growth factors for successful cell division (and  even survival (Raff, 1992)). These factors are nowadays usually referred to as cytokines (Callard &  ...
Protein Expression in the Drosophila Schneider 2 Cell System
Protein Expression in the Drosophila Schneider 2 Cell System

... copies of target DNA in the resulting drug-selected polyclonal population. Although the correlation is generally not 1:1, the greater the target copy number per cell, the more target protein expressed. Despite this, the number of copies for any given cell within a polyclonal population can vary up t ...
The plant formin AtFH4 interacts with both actin and microtubules
The plant formin AtFH4 interacts with both actin and microtubules

... unknown function located between the transmembrane domain and the FH1 domain (Fig. 1A). We have named this region the group Ie (GOE) domain. The GOE domain corresponds to the automatically generated ProDom (Bru et al., 2005) domains PD038281 and PD224441, which are mutually related. The GFPAtFH4D1 c ...
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Cell growth

The term cell growth is used in the contexts of cell development and cell division (reproduction). When used in the context of cell division, it refers to growth of cell populations, where a cell, known as the ""mother cell"", grows and divides to produce two ""daughter cells"" (M phase). When used in the context of cell development, the term refers to increase in cytoplasmic and organelle volume (G1 phase), as well as increase in genetic material (G2 phase) following the replication during S phase.
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