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Measuring forces and stresses in situ in living tissues
Measuring forces and stresses in situ in living tissues

... KEY WORDS: Mechanics, Morphogenesis, Quantitative biology ...
emboj2011361-sup
emboj2011361-sup

... to 250 ms. Analysis of signal intensities was done in the growth region by measuring average intensities at the plasma membrane. Measured values were corrected for cytoplasmic background. From the corrected values the mean average intensity value was calculated. For velocity and frequency measuremen ...
Cell shape and plasma membrane alterations after - An
Cell shape and plasma membrane alterations after - An

The Rice TAL Effector–Dependent Resistance
The Rice TAL Effector–Dependent Resistance

... triplicate, and the data are presented as means 6 SD. (C) and (D) The expression of Xa10 in IRBB10A at 12 HAI with water (M) or PXO99A strains harboring pHM1 (V), pHM1avrXa10 (Wt), pHM1avrXa10NLS (NLS), or pHM1avrXa10AD (AD). RNA gel blot analysis (C) and real-time RT-PCR (D) were performed similarl ...
Scalable Recombinant Adeno-Associated Virus Production
Scalable Recombinant Adeno-Associated Virus Production

... spectrum of diseases (Aucoin et al., 2008). AAV is a dependovirus whose full life cycle is complemented by a helper virus, most often adenovirus or herpes simplex virus (Muzyczka and Berns, 2001). In addition, AAV wild-type infections lack pathological effects, conferring an added measure of safety ...
Single-Cell Isolation and Gene Analysis: Pitfalls and
Single-Cell Isolation and Gene Analysis: Pitfalls and

Extracellular Electrical Fields Direct Wound Healing and Regeneration
Extracellular Electrical Fields Direct Wound Healing and Regeneration

... disturbs the aforementioned processes, while enhancement increases the rate of wound closure and the extent of regeneration. EFs are applied to humans in the clinic, to provide an overwhelming signal for the enhancement of healing of chronic wounds. Although clinical trials, spanning a course of dec ...
Toll-like receptor signaling in cell proliferation and survival
Toll-like receptor signaling in cell proliferation and survival

... transcription factor activation. In addition to driving inflammatory responses, TLRs also regulate cell proliferation and survival which serves to expand useful immune cells and integrate inflammatory responses and tissue repair processes. In this context, central TLR signaling molecules, such as the ...
Transient pluripotent cell populations in vivo
Transient pluripotent cell populations in vivo

... origin and identity of pluripotent cell lines are likely to affect their growth and differentiation characteristics in vitro. Recognition of pluripotent cell lines in vitro, identification of their closest embryological equivalents and investigation of their interrelationships within and across spec ...
Cells in Physcomitrella patens
Cells in Physcomitrella patens

... changed more than four-fold and their expression correlated with the reprogramming phase. Gene ontology (GO) and pathway enrichment analysis of differentially expressed genes (DEGs) identified a set of significantly enriched GO terms and pathways, most of which were associated with photosynthesis, p ...
PPT - 서울대 Biointelligence lab
PPT - 서울대 Biointelligence lab

... rates, and a neuron that distinguish between sequences could transmit a larger number of possible signals.  It is less clear how a temporal code could be ‘read’ by downstream neurons.  1. Temporal integration code: a neuron’s firing rate at any particular moment reflects the summed firing rates of ...
Cell Biology - Educational Services
Cell Biology - Educational Services

... In 1858, after using microscopes much better than Hooke’s first microscope, Rudolf Virchow developed the hypothesis that cells only come from other cells. For example, bacteria, which are single-celled organisms, divide in half (after they grow some) to make new bacteria. In the same way, your body ...
fine structure and morphogenic movements in the
fine structure and morphogenic movements in the

... another mesodermal cell. Some correlations between fine structure and the mechanics of gastrulation are discussed, and a theory of invagination is proposed, based on contraction and expansion of the dense layer and the tight junctions at distal cell surfaces. INTRODUCTION The ordered movement of emb ...
Paclitaxel-induced microtubule stabilization causes mitotic block
Paclitaxel-induced microtubule stabilization causes mitotic block

Muscle Cells - Circulation Research
Muscle Cells - Circulation Research

... RT-PCR to confirm the presence of the sense message; for a second set of samples, the 5' amplimer was used during the reverse transcription step to examine the possibility that an antisense message was transcribed. As seen in Figure 4, both sense and antisense products were found for all ages. Discu ...
The New Introductory Biology Core at UNM
The New Introductory Biology Core at UNM

... sequence of four courses that will be required of all biology majors. This new Biology Core will consist of a sequence of four courses: 201. Molecular and Cell Biology. (4) The scientific method, the role of water in cell biology, carbon and molecular diversity, macromolecules, introduction to metab ...
Populus endobetamannanase PtrMAN6 plays a role in coordinating
Populus endobetamannanase PtrMAN6 plays a role in coordinating

... structure and stockpile the majority of photosynthesisfixed carbon and solar energy. CWs can be generally classified into the primary cell wall (PCW) and secondary cell wall (SCW), which are formed through different processes and are regulated via different pathways. The PCW begins to form along wit ...
Root cytoskeleton: its role in perception of and response to gravity
Root cytoskeleton: its role in perception of and response to gravity

... gravity is also indicated by their active role in signal transduction across the cell periphery (Sastry and Horwitz 1993; Pavalko and Otey 1994) and in controlling the spatial organization of MFs (Miyamoto et al. 1995). Importantly, the integrity of F-actin networks was shown to a€ect formation of t ...
Functional analysis of cardiomyocytes carrying mutations in SCN5A
Functional analysis of cardiomyocytes carrying mutations in SCN5A

... calcium ions from intracellular stores (sarcoplasmic reticulum), binding of calcium to sarcomeric proteins and finally in cardiac muscle contraction. The most important ions, which take part in the action potential generation, are sodium, calcium and potassium. The rapid flow of sodium ions into the ...
Assembly and function of AP-3 complexes in cells expressing
Assembly and function of AP-3 complexes in cells expressing

... but the 2 hinge and ear domains (3A2); two 3A deletion mutants (3A 807–831 and 3A807 stop); and a 3A point mutant (3A817AAA). The 3A 807–831 mutant is missing a fragment of the distal part of the hinge, including the clathrin binding domain (amino acids 817–822), whereas the 3A807stop muta ...
Idh paper revised II - G3: Genes | Genomes | Genetics
Idh paper revised II - G3: Genes | Genomes | Genetics

... death. This death is induced by a pulse of the steroid hormone ecdysone that takes place at the end of the prepupal period. For many years, it has been thought that the ecdysone direct response gene Eip93F (E93) plays a critical role in initiating salivary gland cell death. This conclusion was ...
Prm1p, a Pheromone-regulated Multispanning Membrane Protein
Prm1p, a Pheromone-regulated Multispanning Membrane Protein

... host cell plasma membrane, whereas the SNARE complex assembles from subunits associated with different bilayers— their final structures bear remarkable similarities (Weber et al., 1998). In each case, the assembled fusase has domains in- ...
Bacteria Section 18.2 Summary – pages 484-495
Bacteria Section 18.2 Summary – pages 484-495

... Strong/Thick cell wall 2. The above bacteria grows in chains and the cells are round. What is the name of this bacteria? Strepto-coccus ...
Sample
Sample

... 8. How are active transport and facilitated diffusion similar? Both use an integral protein carrier to move a substance through the membrane. ...
Cell shrinkage and apoptosis: a role for potassium and sodium ion
Cell shrinkage and apoptosis: a role for potassium and sodium ion

... apoptotic body formation (Figure 1A). These changes can be readily detected in most cells by their light scattering properties on a flow cytometer (Figure 1B), where changes in cell volume are thought to be the consequence of extrusion of water from the cell in early apoptosis, prior to apoptotic bo ...
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Cell growth

The term cell growth is used in the contexts of cell development and cell division (reproduction). When used in the context of cell division, it refers to growth of cell populations, where a cell, known as the ""mother cell"", grows and divides to produce two ""daughter cells"" (M phase). When used in the context of cell development, the term refers to increase in cytoplasmic and organelle volume (G1 phase), as well as increase in genetic material (G2 phase) following the replication during S phase.
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