Extracellular Mg concentration and Ca blockers modulate the initial
... USA) and 0.05 mM 2-mercaptoethanol (Sigma Aldrich). After 24 h, non-adherent cells were removed and fresh medium was added. Adherent cells were used in the experiments after 10 days of culture [21]. Mouse lymphocyte-like cell line D10.G4.1 (cat. no. TIB224, American Type Culture Collection, Manassas ...
... USA) and 0.05 mM 2-mercaptoethanol (Sigma Aldrich). After 24 h, non-adherent cells were removed and fresh medium was added. Adherent cells were used in the experiments after 10 days of culture [21]. Mouse lymphocyte-like cell line D10.G4.1 (cat. no. TIB224, American Type Culture Collection, Manassas ...
Myocardial tissue engineering with cells derived from human
... the engineered channels of the scaffolds generated for this report, and synchronized beating was observed as early as one day after cell seeding, which suggests that individual cells interacted with the features of the scaffold, and that inter-channel coupling mechanisms were quickly established. Th ...
... the engineered channels of the scaffolds generated for this report, and synchronized beating was observed as early as one day after cell seeding, which suggests that individual cells interacted with the features of the scaffold, and that inter-channel coupling mechanisms were quickly established. Th ...
Cagayan State University SCIENCE HIGH SCHOOL Sanchez Mira
... Define the meaning of protoplasm and its composition. Compare the different properties of protoplasm. Differentiate the properties of protoplasm. Identify the different parts and functions of a cell. Discuss the cell theory. Enumerate the different types of cell. Explain the cell divis ...
... Define the meaning of protoplasm and its composition. Compare the different properties of protoplasm. Differentiate the properties of protoplasm. Identify the different parts and functions of a cell. Discuss the cell theory. Enumerate the different types of cell. Explain the cell divis ...
Ultrastructure of cell types of the olfactory epithelium in a catfish
... lamella. These cells are also columnar and the free broad end is provided with a number of cilia (figures 1, 9). The length of the cilia could not be measured but the diameter is about 0·25 µm. These are typical kinocilia possessing 9 + 2 axial pattern of microtubules. The basal bodies are present b ...
... lamella. These cells are also columnar and the free broad end is provided with a number of cilia (figures 1, 9). The length of the cilia could not be measured but the diameter is about 0·25 µm. These are typical kinocilia possessing 9 + 2 axial pattern of microtubules. The basal bodies are present b ...
cytochemical localization of acid phosphatases in euglena gracilis
... that some constitutive acid phosphatase activity is associated with the paramylum bodies. The appearance of a lead sulfide precipitate in the absence of added substrate, probably indicates the existence of an endogenous source of phosphate in the paramylum bodies. In induced cell preparations treate ...
... that some constitutive acid phosphatase activity is associated with the paramylum bodies. The appearance of a lead sulfide precipitate in the absence of added substrate, probably indicates the existence of an endogenous source of phosphate in the paramylum bodies. In induced cell preparations treate ...
Hemolytic Anemias
... • A group of disorders leading to anemia caused by a reduction in red cell life span. • RBC’s normally survive 60 - 120 days . ...
... • A group of disorders leading to anemia caused by a reduction in red cell life span. • RBC’s normally survive 60 - 120 days . ...
Lab 4
... Lab 4 Lab 3 Goals and Objectives • Learn methods of differential staining: – Use two or more stains and categorize cells into groups – Gram Staining – Separates bacteria in two different groups – Gram positive and Gram negative – Important first test for bacterial identification ...
... Lab 4 Lab 3 Goals and Objectives • Learn methods of differential staining: – Use two or more stains and categorize cells into groups – Gram Staining – Separates bacteria in two different groups – Gram positive and Gram negative – Important first test for bacterial identification ...
2014 Biology STAAR EOC Review
... When HIV attacks a helper T cell, it binds to the cell membrane and enters the cell. Once the virus is inside the cell, it uses the cell’s structures to make new viruses. Then the virus destroys the cell and the new viruses are released into the bloodstream. They travel throughout the blood, infecti ...
... When HIV attacks a helper T cell, it binds to the cell membrane and enters the cell. Once the virus is inside the cell, it uses the cell’s structures to make new viruses. Then the virus destroys the cell and the new viruses are released into the bloodstream. They travel throughout the blood, infecti ...
CELL BIOLOGY - BIOL 303 EXAM 1 There is only 1 correct answer
... In SDS polyacrylamide-gel electrophoresis individual protein migrate in the electrical field at rates determined by their molecular weight: the larger the protein the more it is retarded by the complex meshwork of polyactylarnide molecules that constitutes the gel and therefore the more slowly it mi ...
... In SDS polyacrylamide-gel electrophoresis individual protein migrate in the electrical field at rates determined by their molecular weight: the larger the protein the more it is retarded by the complex meshwork of polyactylarnide molecules that constitutes the gel and therefore the more slowly it mi ...
Microautophagy and macropexophagy may occur
... presence of glucose (Fig. 2A). Organelle degradation, demonstrated by the accumulation of peroxisomal AO into vacuoles by immunocytochemistry, was evident already after 30 min of incubation (Fig. 2F). Additionally, exposure of cells to nitrogen-limiting conditions showed the expected morphology of o ...
... presence of glucose (Fig. 2A). Organelle degradation, demonstrated by the accumulation of peroxisomal AO into vacuoles by immunocytochemistry, was evident already after 30 min of incubation (Fig. 2F). Additionally, exposure of cells to nitrogen-limiting conditions showed the expected morphology of o ...
Bacterial Cell Morphogenesis Does Not Require a Preexisting
... colonies appeared (Figure 4B, right). These colonies contained many walled cells with typical rod-shape morphology (Figure 4Dii). The colony-purified strain was able to grow with normal rod shape on NA plates (without osmoprotectants) in the presence of IPTG (and xylose, for the expression of cdsA) ...
... colonies appeared (Figure 4B, right). These colonies contained many walled cells with typical rod-shape morphology (Figure 4Dii). The colony-purified strain was able to grow with normal rod shape on NA plates (without osmoprotectants) in the presence of IPTG (and xylose, for the expression of cdsA) ...
Alfred Hershey and Martha Chase (presented by Kristy)
... Delbruck and Luria, Hershey used their findings to produce his skillful experimentation along with Martha Chase. ...
... Delbruck and Luria, Hershey used their findings to produce his skillful experimentation along with Martha Chase. ...
User Manual - System Biosciences
... applications, advantages, and limitations that can be complementary to other modalities. For example, a cell-based technique (with GFP or RFP) has limitations for whole body in vivo imaging studies, whereas techniques involved in imaging at the organ level (with Luciferase or TK) do not have the res ...
... applications, advantages, and limitations that can be complementary to other modalities. For example, a cell-based technique (with GFP or RFP) has limitations for whole body in vivo imaging studies, whereas techniques involved in imaging at the organ level (with Luciferase or TK) do not have the res ...
C-Cure®: first-in-class reparative therapy for heart failure treatment
... mechanism called metastasis. Cancer generally forms a solid tumor mass (such as lung or colon cancers). Some cancers like leukemia (blood cancer) or lymphoma (bone marrow cancer) do not form masses, and are called liquid tumors. Cancer treatments in the last century have always been a balance betwee ...
... mechanism called metastasis. Cancer generally forms a solid tumor mass (such as lung or colon cancers). Some cancers like leukemia (blood cancer) or lymphoma (bone marrow cancer) do not form masses, and are called liquid tumors. Cancer treatments in the last century have always been a balance betwee ...
Loss of Growth Factor Dependence and Conversion of Transforming
... ABSTRACT Cell lines with varying tumorigenic and metastatic potentials have been obtained by transformation of KIT1•¿ fibroblasts using radiation or transfection with 1-24 H-ras. We have observed an inverse relationship between metastatic potential and dependence on serum for growth. The effects o ...
... ABSTRACT Cell lines with varying tumorigenic and metastatic potentials have been obtained by transformation of KIT1•¿ fibroblasts using radiation or transfection with 1-24 H-ras. We have observed an inverse relationship between metastatic potential and dependence on serum for growth. The effects o ...
SET1 - CBSE
... Ans.. Divergent evolution/ Homologous organs, Similar in origin but perform different function ...
... Ans.. Divergent evolution/ Homologous organs, Similar in origin but perform different function ...
Physico-chemical characteristics of cell walls from Arabidopsis
... percentage of total wall galactose released by the endo(1,4)-b-D-galactanase gradually decreased during the cell culture. The a-L-arabinofuranosidase released about 50% of total wall arabinose at the three growth phases, but bD-galactosidase released no galactose. In order to test whether these enzy ...
... percentage of total wall galactose released by the endo(1,4)-b-D-galactanase gradually decreased during the cell culture. The a-L-arabinofuranosidase released about 50% of total wall arabinose at the three growth phases, but bD-galactosidase released no galactose. In order to test whether these enzy ...
Magic Lysis Buffer Improves the Efficiency of
... Immunoprecipitation (IP) - Tandem Mass Spectrometry (IP-LC/MS/MS) has long suffered from contamination with non-specific protein interactions that suppress true bait-prey binding partners. In addition, caution must be taken about the stringency of lysis buffer since it can strip the bait protein of ...
... Immunoprecipitation (IP) - Tandem Mass Spectrometry (IP-LC/MS/MS) has long suffered from contamination with non-specific protein interactions that suppress true bait-prey binding partners. In addition, caution must be taken about the stringency of lysis buffer since it can strip the bait protein of ...
The Diversity of Viruses, Prokaryotes and Protists 2
... Resistant to extreme conditions Can survive for “extraordinarily” long periods ...
... Resistant to extreme conditions Can survive for “extraordinarily” long periods ...
Nephrogenesis
... Microculture of metanephrogenic unit. To study MET, a nephrogenic unit as defined by a single ureteric bud with induced adherent mesenchyme is transferred in collagens. Schematic view illustrates in vitro MET and early nephrogenesis.These processes are documented by electron microscopy and by molec ...
... Microculture of metanephrogenic unit. To study MET, a nephrogenic unit as defined by a single ureteric bud with induced adherent mesenchyme is transferred in collagens. Schematic view illustrates in vitro MET and early nephrogenesis.These processes are documented by electron microscopy and by molec ...
Accompanying protein alterations in malignant cells with a
... Utilizing parental and drug-resistant ovarian carcinoma cell lines that have acquired point mutations in -tubulin and p53, we studied the level of expression and modification of proteins involved in apoptosis and MT integrity. Extending previous results, we demonstrated phosphorylation of pro-survi ...
... Utilizing parental and drug-resistant ovarian carcinoma cell lines that have acquired point mutations in -tubulin and p53, we studied the level of expression and modification of proteins involved in apoptosis and MT integrity. Extending previous results, we demonstrated phosphorylation of pro-survi ...
1 Collaborative Control of Cell Cycle Progression by the
... analysis. Each experimental replicate was performed using freshly generated and circularized single strand RNA. ...
... analysis. Each experimental replicate was performed using freshly generated and circularized single strand RNA. ...
Microbiology Laboratory Safety and Basic Procedures Safety in a
... chemoheterotrophs. For example, nutrient agar consists of beef extract, peptone, and agar all dissolved in water; it can be used to culture many commonly encountered organisms. Certain other organisms have more complicated requirements for certain compounds and are called fastidious chemoheterotroph ...
... chemoheterotrophs. For example, nutrient agar consists of beef extract, peptone, and agar all dissolved in water; it can be used to culture many commonly encountered organisms. Certain other organisms have more complicated requirements for certain compounds and are called fastidious chemoheterotroph ...
Multiple mechanisms, roles and controls of K+ transport in
... estimate the magnitude of this parameter. One method simply adds up the concentration of cytoplasmic solutes, assuming that the activity coefficients are similar to those in free solutions of similar composition. However, a fraction of any cation pools in the cell are involved in balancing the charg ...
... estimate the magnitude of this parameter. One method simply adds up the concentration of cytoplasmic solutes, assuming that the activity coefficients are similar to those in free solutions of similar composition. However, a fraction of any cation pools in the cell are involved in balancing the charg ...
Microtubule-Dependent Regulation of α2B Adrenergic Receptors in
... nucleotide-sensitive high-affinity receptor-agonist interactions (Williams and Lefkowitz, 1977; Gerhardt et al., 1990). To evaluate the ability of Gpp(NH)p to modulate radiolabeled agonist binding, [125I]PIC incubations were performed in the absence (control) or presence of increasing concentrations ...
... nucleotide-sensitive high-affinity receptor-agonist interactions (Williams and Lefkowitz, 1977; Gerhardt et al., 1990). To evaluate the ability of Gpp(NH)p to modulate radiolabeled agonist binding, [125I]PIC incubations were performed in the absence (control) or presence of increasing concentrations ...
Cell culture
Cell culture is the process by which cells are grown under controlled conditions, generally outside of their natural environment. In practice, the term ""cell culture"" now refers to the culturing of cells derived from multicellular eukaryotes, especially animal cells, in contrast with other types of culture that also grow cells, such as plant tissue culture, fungal culture, and microbiological culture (of microbes). The historical development and methods of cell culture are closely interrelated to those of tissue culture and organ culture. Viral culture is also related, with cells as hosts for the viruses. The laboratory technique of maintaining live cell lines (a population of cells descended from a single cell and containing the same genetic makeup) separated from their original tissue source became more robust in the middle 20th century.