Aurintricarboxylic Acid Rescues PC12 Cells and Sympathetic
... (1990) . The nucleic acid concentration was determined by UV absorbance at 260 run . The same amount of nucleic acid from each sample (10-30 kg, depending on the experiment) was subjected to electrophoresis on a 1 .2 % agarose gel . Subsequently, the gel was incubated at 37°C with RNase A (20 pg/ml) ...
... (1990) . The nucleic acid concentration was determined by UV absorbance at 260 run . The same amount of nucleic acid from each sample (10-30 kg, depending on the experiment) was subjected to electrophoresis on a 1 .2 % agarose gel . Subsequently, the gel was incubated at 37°C with RNase A (20 pg/ml) ...
Plant cell wall Composition
... provides these cells with structural support and protection. A major function of the cell wall, in multicellular organisms, is to act as a pressure vessel, preventing overexpansion when water enters the cell. In other word the creation of a stable osmotic environment by preventing osmotic lysis and ...
... provides these cells with structural support and protection. A major function of the cell wall, in multicellular organisms, is to act as a pressure vessel, preventing overexpansion when water enters the cell. In other word the creation of a stable osmotic environment by preventing osmotic lysis and ...
Regeneration in plants and animals: dedifferentiation
... mature muscle fibers was confirmed by tracking fluorescently labeled single muscle fibers [24], and by observing mononucleation of muscle fibers, which was reported as the structural marker of a prior state of the cell in classical experiments [25,26]. In support of these cells changing their identi ...
... mature muscle fibers was confirmed by tracking fluorescently labeled single muscle fibers [24], and by observing mononucleation of muscle fibers, which was reported as the structural marker of a prior state of the cell in classical experiments [25,26]. In support of these cells changing their identi ...
Chapter 2: Basic Biological Principles Lesson 2: Structural and
... The answer to these questions is clear once you know how a cell functions. To carry out life processes, a cell must be able to quickly pass substances into and out of the cell. For example, it must be able to pass nutrients and oxygen into the cell and waste products out of the cell. Anything that e ...
... The answer to these questions is clear once you know how a cell functions. To carry out life processes, a cell must be able to quickly pass substances into and out of the cell. For example, it must be able to pass nutrients and oxygen into the cell and waste products out of the cell. Anything that e ...
Microbiology
... 6 describe the differences in bacterial cell wall structure that are the basis of the Gram staining technique. ...
... 6 describe the differences in bacterial cell wall structure that are the basis of the Gram staining technique. ...
loading control antibodies for western blotting
... Western blotting requires such controls: it is widely used for the semi-quantification of protein levels under of a set of different experimental parameters. Protein standards are required to make sense of Western blotting results, and check that any increases and decreases in target proteins are ac ...
... Western blotting requires such controls: it is widely used for the semi-quantification of protein levels under of a set of different experimental parameters. Protein standards are required to make sense of Western blotting results, and check that any increases and decreases in target proteins are ac ...
Determination of the Order of Gene Function in Yeast Nuclear Division Pathway Using cs and ts Mutants.
... cold-sensitivestep; DNA synthesis is required before the CDC44 function in a dependent pathway. In the case of CDC45, exactly the same conclusion can be drawn since shift from cold to HU allows 60% of the cells to pass to a second cell cycle as opposed to none when the shift is from HU to cold; DNA ...
... cold-sensitivestep; DNA synthesis is required before the CDC44 function in a dependent pathway. In the case of CDC45, exactly the same conclusion can be drawn since shift from cold to HU allows 60% of the cells to pass to a second cell cycle as opposed to none when the shift is from HU to cold; DNA ...
Cytokinin Functions
... ability to promote cytokinesis. Hall and deRopp reported that kinetin could be formed from DNA degradation products in 1955 (Hall and deRopp, 1955). 1961: Miller isolated the first naturally occurring cytokinin from corn (Miller, 1961). It was later called zeatin. Almost simultaneous with Miller, Le ...
... ability to promote cytokinesis. Hall and deRopp reported that kinetin could be formed from DNA degradation products in 1955 (Hall and deRopp, 1955). 1961: Miller isolated the first naturally occurring cytokinin from corn (Miller, 1961). It was later called zeatin. Almost simultaneous with Miller, Le ...
Plant hormones and phototropism
... response. The structural and physiological mechanisms involved in this response still pose questions for plant researchers. Our lab today will help us explore what is happening in this response. The hormone involved in this response is an auxin. This chemical messenger is found in the apical meriste ...
... response. The structural and physiological mechanisms involved in this response still pose questions for plant researchers. Our lab today will help us explore what is happening in this response. The hormone involved in this response is an auxin. This chemical messenger is found in the apical meriste ...
A Tour of the Cell
... Antibiotics: Drugs that Target Bacterial Cells • Most antibiotics kill bacteria while minimally harming the human host by binding to structures found only on bacterial cells. • Some antibiotics bind to the bacterial ribosome, leaving human ribosomes unaffected. • Other antibiotics target enzymes fou ...
... Antibiotics: Drugs that Target Bacterial Cells • Most antibiotics kill bacteria while minimally harming the human host by binding to structures found only on bacterial cells. • Some antibiotics bind to the bacterial ribosome, leaving human ribosomes unaffected. • Other antibiotics target enzymes fou ...
Introduction to Biology - Phillips Scientific Methods
... a ring. Although centrioles may help organize microtubule assembly, they are not essential for this function in all eukaryotes (centrosomes of most plants lack centrioles entirely). b. Cilia and flagella – are extensions of eukaryotic cells, and are composed of, and move by, microtubules. Cilia are ...
... a ring. Although centrioles may help organize microtubule assembly, they are not essential for this function in all eukaryotes (centrosomes of most plants lack centrioles entirely). b. Cilia and flagella – are extensions of eukaryotic cells, and are composed of, and move by, microtubules. Cilia are ...
Laser-Micropipet Combination for Single-Cell Analysis
... (20 nM) and/or Oregon Green (500 nM) diacetates were made in buffer A with glucose (10 mM) immediately prior to use and added to the cell chamber after removing the wash buffer. The cells were incubated at room temperature in the dark for 30 min to load the fluorescent compounds. The cells were then ...
... (20 nM) and/or Oregon Green (500 nM) diacetates were made in buffer A with glucose (10 mM) immediately prior to use and added to the cell chamber after removing the wash buffer. The cells were incubated at room temperature in the dark for 30 min to load the fluorescent compounds. The cells were then ...
Daratumumab-mediated lysis of primary multiple
... Despite recent treatment improvements, multiple myeloma remains an incurable disease. Since antibody-dependent cell-mediated cytotoxicity is an important effector mechanism of daratumumab, we explored the possibility of improving daratumumab-mediated cell-mediated cytotoxicity by blocking natural ki ...
... Despite recent treatment improvements, multiple myeloma remains an incurable disease. Since antibody-dependent cell-mediated cytotoxicity is an important effector mechanism of daratumumab, we explored the possibility of improving daratumumab-mediated cell-mediated cytotoxicity by blocking natural ki ...
CELL STRUCTURE_2012
... Protein assisted movement down a concentration gradient molecule binds to its carrier protein, potentially changing its shape, and is carried to the other side ...
... Protein assisted movement down a concentration gradient molecule binds to its carrier protein, potentially changing its shape, and is carried to the other side ...
Isolation and Pure Culture of a Freshwater Magnetic Spirillum in
... buffering characteristics, temperature, oxygen concentration, chemical reducing agents, and various iron and nitrogen sources were determined, and the results were used as a basis in constructing an isolation medium. Isolation and growth of strain MS-1. Inocula for isolating magnetic bacteria were o ...
... buffering characteristics, temperature, oxygen concentration, chemical reducing agents, and various iron and nitrogen sources were determined, and the results were used as a basis in constructing an isolation medium. Isolation and growth of strain MS-1. Inocula for isolating magnetic bacteria were o ...
Quiz Show A
... why normal cells grown in a Petri dish tend to stop growing once they have covered the bottom of the dish? a. The cells lack cyclin. b. The Petri dish inhibits growth. c. Contact with other cells stops cell growth. d. Most cells grown in Petri dishes have defective p53. ...
... why normal cells grown in a Petri dish tend to stop growing once they have covered the bottom of the dish? a. The cells lack cyclin. b. The Petri dish inhibits growth. c. Contact with other cells stops cell growth. d. Most cells grown in Petri dishes have defective p53. ...
A Diffusible Signal from Arbuscular Mycorrhizal Fungi Elicits a
... short contact with ungerminated spores, the medium triggered a detectable [Ca21]cyt change (Fig. 3). The maximal amplitude of the Ca21 peak was reached after 8 d, and thereafter only a slight modulation of the Ca21 signal was recorded (Fig. 3). The fungal medium, renewed after 8 d and collected at 1 ...
... short contact with ungerminated spores, the medium triggered a detectable [Ca21]cyt change (Fig. 3). The maximal amplitude of the Ca21 peak was reached after 8 d, and thereafter only a slight modulation of the Ca21 signal was recorded (Fig. 3). The fungal medium, renewed after 8 d and collected at 1 ...
Basic Science for Clinicians
... Especially interesting to the scope of this review is the involvement of Hh in the morphogenesis of the heart and blood vessels. A clue to this involvement was the ability of Shh to induce vascular endothelial growth factor and angiopoietins in human fibroblasts,30 but a more definite answer came fr ...
... Especially interesting to the scope of this review is the involvement of Hh in the morphogenesis of the heart and blood vessels. A clue to this involvement was the ability of Shh to induce vascular endothelial growth factor and angiopoietins in human fibroblasts,30 but a more definite answer came fr ...
Mesoderm induction in Xenopus laevis:responding
... (1986) have correlated such a depression in cytokeratin gene expression with mesoderm induction, pointing out that the induction process diverts cells away from their original epidermal specification. The suppression of epidermal differentiation observed in response to XTC-MIF may therefore represen ...
... (1986) have correlated such a depression in cytokeratin gene expression with mesoderm induction, pointing out that the induction process diverts cells away from their original epidermal specification. The suppression of epidermal differentiation observed in response to XTC-MIF may therefore represen ...
Sickle Cell Workshop
... In HbA HbS individuals, half their hemoglobin will sickle when the oxygen tension becomes very low. These sickled cells are removed from the body by the spleen, along with the merozoites inside of them. Thus, heterozygotes remove the infected cells from their body before the protozoans can produce a ...
... In HbA HbS individuals, half their hemoglobin will sickle when the oxygen tension becomes very low. These sickled cells are removed from the body by the spleen, along with the merozoites inside of them. Thus, heterozygotes remove the infected cells from their body before the protozoans can produce a ...
Preparing Plant Tissue Culture Medium Micronutrients
... cut into small pieces that will give rise to many more individual plants. Soil or hydroponically grown plants only need fertilizer (a source of K, N and P plus trace minerals), water, air and light to grow, because they can make their own sugar, amino acids, etc. • Plant tissue cultures need an outs ...
... cut into small pieces that will give rise to many more individual plants. Soil or hydroponically grown plants only need fertilizer (a source of K, N and P plus trace minerals), water, air and light to grow, because they can make their own sugar, amino acids, etc. • Plant tissue cultures need an outs ...
Peroxiredoxin-controlled G-CSF signalling at the endoplasmic
... of H2O2 for the so-called oxidative burst, a major weapon in host defence against bacteria. In non-phagocytic cells, H2O2 has long been considered as an unwanted by-product of cell metabolism, potentially hazardous because of the damaging effects on proteins, lipids, carbohydrates and nucleic acids ...
... of H2O2 for the so-called oxidative burst, a major weapon in host defence against bacteria. In non-phagocytic cells, H2O2 has long been considered as an unwanted by-product of cell metabolism, potentially hazardous because of the damaging effects on proteins, lipids, carbohydrates and nucleic acids ...
Cell culture
Cell culture is the process by which cells are grown under controlled conditions, generally outside of their natural environment. In practice, the term ""cell culture"" now refers to the culturing of cells derived from multicellular eukaryotes, especially animal cells, in contrast with other types of culture that also grow cells, such as plant tissue culture, fungal culture, and microbiological culture (of microbes). The historical development and methods of cell culture are closely interrelated to those of tissue culture and organ culture. Viral culture is also related, with cells as hosts for the viruses. The laboratory technique of maintaining live cell lines (a population of cells descended from a single cell and containing the same genetic makeup) separated from their original tissue source became more robust in the middle 20th century.