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Identification of trichoplein, a novel keratin filament
Identification of trichoplein, a novel keratin filament

... similarity to trichohyalin, plectin and myosin heavy chain, and is a K8/18-binding protein. Among interactions between trichoplein and various IF proteins that we tested using two-hybrid methods, trichoplein interacted significantly with K16 and K18, and to some extent with K5, K6a, K8 and K14. In i ...
High sensitivity of embryonic stem cells to
High sensitivity of embryonic stem cells to

... involved in the ubiquitination and proteasome pathway was found to be expressed in human ES cells and to be decreased upon differentiation (24). Therefore, we propose that the ubiqutin proteasomal system in ES cells is required not only for fine tuning as a negative regulator through degradation, bu ...
PDF
PDF

... Fig. 2. Mesoderm induction by a pellet of XTC cells but not by a pellet of XL cells. Isolated Xenopus blastula animal pole regions were pressed against a pellet of XTC or XL cells and allowed to develop for 65 h at 18-22 °C in modified L15 medium containing 10 % foetal calf serum. After fixation and ...
Mechanobiology of bone cells
Mechanobiology of bone cells

... Downloaded from www.osteologie-journal.de on 2017-06-17 | IP: 88.99.165.207 For personal or educational use only. No other uses without permission. All rights reserved. ...
repair (healing)
repair (healing)

... • Following vasoconstriction, platelets adhere to damaged endothelium and discharge adenosine diphosphate (ADP), promoting thrombocyte clumping, which dams the Wound • The inflammatory phase is initiated by the release of numerous cytokines by platelets. • Fibrinogen is cleaved into fibrin and the f ...
A. diffuser
A. diffuser

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In-situ and label-free optical monitoring of the adhesion and
In-situ and label-free optical monitoring of the adhesion and

... al., 2007)) are available. Moreover, these substrata can be easily surface-modified or coated with a thin layer of virtually any material, provided it is transparent at the wavelength of the guided light. This gives a tremendous flexibility to the technique. ...
Batteries (cells with spontaneous redox reactions)
Batteries (cells with spontaneous redox reactions)

... Can’t recharge because Zn2+ diffuses away. Acidic NH4+ corrodes Zn, leading to poor shelf life. ...
Lecture Slides
Lecture Slides

... Lectures by Chris C. Romero, updated by Edward J. Zalisko © 2010 Pearson Education, Inc. ...
Basic Theories for Introductory Biology
Basic Theories for Introductory Biology

... 2013 for teachers to use, at the request of Jane Jackson, Co-Director of the Modeling Instruction Program at ASU. For personal use only. Permission is not granted for any other use. Do not post on a website.] A note by Anton “Tony” Lawson: My definition of a theory is an explanation for a broad clas ...
Distinct Cellular and Subcellular Patterns of Expression Imply
Distinct Cellular and Subcellular Patterns of Expression Imply

... maintenance of microvilli, cell-cell adhesion, and cell-substrate adhesion (Takeuchi et al., 1994). Interest in the ERM family has been stimulated by the discovery that human neurofibromatosis 2 (NF2) is caused by mutations in merlin (moesin-ezrin-radixin-like protein), a novel member of the ERM fam ...
Ch3-4.Embryology.Tissues.Lecture
Ch3-4.Embryology.Tissues.Lecture

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Cell Organelles and Features
Cell Organelles and Features

... Copyright © by Holt, Rinehart and Winston. All rights reserved. ...
MARKER GENE TECHNOLOGIES, Inc
MARKER GENE TECHNOLOGIES, Inc

... groups of compounds for the study of live cells. Modification of carboxylic acids with AM ester groups results in an uncharged molecule that can permeate cell membranes. Once inside the cell, the lipophilic blocking groups are cleaved by nonspecific esterases, resulting in a charged form that leaks ...
Review guide – Trimester 1 Finals General Biology – 2012 Chapter
Review guide – Trimester 1 Finals General Biology – 2012 Chapter

... Explain with a diagram or words how and why molecules will diffuse across a membrane or through a medium. How do proteins work to facilitate diffusion? When does osmosis occur and how is it different or similar to diffusion? Compare and contrast the response of animal and plant cells to immersion in ...
Chapter 12: Neural Tissue
Chapter 12: Neural Tissue

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Receptor-Mediated Endocytosis in Plant Cells
Receptor-Mediated Endocytosis in Plant Cells

... fluorescence image of the same field showing the location of the fluorescent elicitor (b, d, f, h, j, I, n, p, and r). As seen in the micrographs, soon after addition of the elicitor, sufficient amounts of the ligand accumulated at the cell surface to form a fluorescent outline surrounding each cell ...
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Chapter 35.

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Tutorial 8 – Cytoskeleton
Tutorial 8 – Cytoskeleton

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Fluorescent probes for plant imaging
Fluorescent probes for plant imaging

... for pollen grains and cultured plant cells.57,58 Although not tested in our labs, calcein AM, a cell-permeant dye that has been tested in many eukaryotic cells, is likely to work in plants as well. In addition, other AM ester dyes have the potential to be used in plants for viability staining. For v ...
Spherical Solar Cells Solve Issue of 3
Spherical Solar Cells Solve Issue of 3

... on the opposite side of a light source compared with its output measured without a reflection plate. This means that there is ample room for a spherical micro solar cell to improve its output by devising effective ways of collecting reflected light. The light-electricity conversion efficiency of a t ...
The nucleolar structure and nucleolar proteins as indicators of cell
The nucleolar structure and nucleolar proteins as indicators of cell

... mammals and yeast (Guiltinan et al. 1988, Cerdido and Medina 1995). Studies on the fibrillarin gene after its molecular cloning in Arabidopsis, have demonstrated that its expression is regulated by hormones, such as abscisic acid, and it is also modulated by aging (Pih et al. 2000). The levels of fi ...
The Plant Endomembrane System—A Complex
The Plant Endomembrane System—A Complex

... The levels and distribution of the plant hormone auxin are critical in regulating multiple developmental processes in plants (Mockaitis and Estelle 2008, Vanneste and Friml 2009). Auxin transporters of the PIN-FORMED (PIN) family localize asymmetrically at the PM and mediate directional intercellula ...
Mitochondrial inheritance is mediated by microtubules in
Mitochondrial inheritance is mediated by microtubules in

pLV-RNAi - Biosettia
pLV-RNAi - Biosettia

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Cell culture



Cell culture is the process by which cells are grown under controlled conditions, generally outside of their natural environment. In practice, the term ""cell culture"" now refers to the culturing of cells derived from multicellular eukaryotes, especially animal cells, in contrast with other types of culture that also grow cells, such as plant tissue culture, fungal culture, and microbiological culture (of microbes). The historical development and methods of cell culture are closely interrelated to those of tissue culture and organ culture. Viral culture is also related, with cells as hosts for the viruses. The laboratory technique of maintaining live cell lines (a population of cells descended from a single cell and containing the same genetic makeup) separated from their original tissue source became more robust in the middle 20th century.
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