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microscopy technique-2
microscopy technique-2

... An inoculum is spread across the surface of an agar plate in a sequential pattern of streak (as indicated by the numbers n arrows) The loop is sterilized between streaks In streak 2,3 and 4, bacteria are picked up from previous streak, diluting the number of cells each time These develop into separa ...
The Endocrine System
The Endocrine System

... considered the most important part of the endocrine system. • It's often called the "master gland" because it makes hormones that control several other endocrine glands. • The production and secretion of pituitary hormones can be influenced by factors such as emotions and seasonal changes. • Among t ...
3-3, 3-4, 3-5 membrane, diff, trans
3-3, 3-4, 3-5 membrane, diff, trans

... Amino acids ...
A-P Chapter 3
A-P Chapter 3

... which water moves from an area of greater water concentration (where there is less osmotic pressure) across a selectively permeable membrane to an area of lower water concentration (where there is greater ...
Companion Cells
Companion Cells

... of most organelles and by developing a parietal anastomosed endoplasmic reticulum system and sieve plates between sieve elements. The result of sieve element differentiation is a system of interconnected, enucleate, living cells with all organelles anchored to the plasma membrane, leaving the central ...
Puberty and the oestrus cycle
Puberty and the oestrus cycle

... is controlled by GnRHs (gonadotropin-releasing hormones) that are secreted from the hypothalamus and conveyed to the anterior pituitary gland through hypothalamo-hypophyseal portal blood circulation. Secretion of GnRHs, and thus of FSH and LH is influenced by visual, olfacrory, auditory and tactile ...
Hyperosmotic stress-induced actin filament reorganization in leaf
Hyperosmotic stress-induced actin filament reorganization in leaf

... hypertonic solution and that plasmolysis is completed within 1±5 min after immersion of the leaf segments in the plasmolytic solution. After 5 min the protoplast volume and shape remain fairly constant (Fig. 1A±D). The same plasmolysis pattern was induced by 0.5 and 0.3 M mannitol solutions. However ...
Lab Test 1 Study Sheet (summer term)
Lab Test 1 Study Sheet (summer term)

... Have an understanding of the following topics: 24. Be able to describe the surface area to volume ratio of cells. 25. Recognize a cheek cell, and know some of the typical cellular organelles. 26. Know the differences in light microscope and electron scope. 27. What are the steps of the scientific me ...
Water movement between epidermal cells of barley leaves – a
Water movement between epidermal cells of barley leaves – a

... cell (cell-1). The probe was then removed, leaving the cell with a puncture (leak). Within the next 1–6 min, a cell (cell2) adjacent to the leaking cell was analysed for turgor and T1/2. In one set of experiments, the probed cell was located laterally to the punctured (and leaking) cell and the latt ...
CELL STRUCTURE AND FUNCTION CHAPTER3
CELL STRUCTURE AND FUNCTION CHAPTER3

... the cell is alive. Thus, the answer to what life is must lie within the cell, because the smallest living organisms are unicellular, while larger organisms are multicellular—that is, composed of many cells. The diversity of cells is exemplified by the many types in the human body, such as muscle cel ...
10-2
10-2

... HUMAN CELLS THAT KEEP DIVIDING ...
Nanomechanics of superbugs and superdrugs
Nanomechanics of superbugs and superdrugs

... movement of the levers (illustrated in Figure 1). By measuring the surface drug-target binding constants on the cantilever arrays, we see that even tiny changes in drug-resistant cell wall structures make it approximately 800-fold harder for the antibiotic to attach itself on to VRE analogues, leavi ...
The role of c-myc in cellular growth control
The role of c-myc in cellular growth control

... types of growth control can be distinguished: (1) `Stringent' control during rapid changes in nutrient availability; (2) `Growth rate controls' during continuous proliferation; and (3) Cell cycle variation in protein synthesis. The existence of the ®rst two di€erent sets of controls is best shown in ...
MCAS Test Questions - Massachusetts Comprehensive Assessment
MCAS Test Questions - Massachusetts Comprehensive Assessment

... Sickle cell anemia is an autosomal recessive genetic disorder that affects thousands of people in the United States and millions worldwide. Sickle cell anemia commonly occurs in groups whose ancestors came from Africa, as well as South America, Cuba, Central America, Saudi Arabia, India, and the Med ...
Jeopardy - Camerado Springs Middle School
Jeopardy - Camerado Springs Middle School

... “Waste disposal plant area” of cells. ...
Effects of excess and deficient boron and niacin
Effects of excess and deficient boron and niacin

... in interstellar dust or during the earth’s early history. As a consequence of its essential role in growing tissues and inherent phloem mobility of B in most plant species, many species are also sensitive to high levels of B in soil and water and growth inhibition has been retarded as a result of ex ...
Introduction: spatial origin of murine hematopoietic stem cells
Introduction: spatial origin of murine hematopoietic stem cells

... sp stage. Rugh39 notes that the heart is beating regularly and the circulation is playing a major role in development by the 13 sp stage (day 9 pc). Therefore, while it is not absolutely clear when forward flow of blood through the yolk sac and embryonic vasculature is accomplished, isolation of the ...
the role of disturbed ph dynamics and the na+/h+ exchanger in
the role of disturbed ph dynamics and the na+/h+ exchanger in

... intracellular pH (pHi) in cancer cells involved the use of NIH-3T3 cells transformed with the E7 oncoprotein of the malignant human papilloma virus type 16 under the control of a tetracycaline (tet) inducible promoter 9. The use of this inducible expression system permitted a finely controlled study ...
Student Edition Sample Chapter (3MB PDF)
Student Edition Sample Chapter (3MB PDF)

... The nucleus is The most visible organelle in a eukaryotic cell is the nucleus. The the control center nucleus is covered with a membrane that allows materials to pass in and out. It’s often called the “control center” of the cell because it contains DNA. As you have learned, DNA is the hereditary ma ...
Journal of Bacteriology
Journal of Bacteriology

... were prepared as described above. The cells were contrasted by the glutaraldehyde-ruthenium red-uranyl acetate (G/RR/ UA) procedure (24). In some cases, bacteria were pretreated for 30 min at room temperature with immunoaffinity-purified trifoliin A (12) coupled with colloidal gold (20) before treat ...
Mycoplasma
Mycoplasma

...  These organisms, called L-forms, can also be formed by many species when cell wall synthesis is impaired by antibiotic treatment or high salt concentration. ...
The mechanism of leaf morphogenesis
The mechanism of leaf morphogenesis

... variable. Cells in a meristem are maintained at a particular size, but as cells are removed from the meristem this balance between size and division is shifted. Moreover, this shift occurs in a tissue-specific manner (in fact, in some ways defines tissue identity). The mechanism controlling this shift ...
Vascular cell adhesion molecule-1 induced by human T
Vascular cell adhesion molecule-1 induced by human T

... cells. To determine whether VCAM-1 induced by Tax is involved in the proliferation of CD3-stimulated Tlymphocytes, we performed blocking experiments in which Tax-expressing Jurkat cells were pre-treated with 10 µg\ml VCAM-1-blocking MAb for 30 min at 4 mC, fixed and then cocultivated with CD3-stimul ...
Microsoft Word 97
Microsoft Word 97

... reached the ends of their life spans. Our overall body conditions at particular times depend upon cells reproducing and the rates at which they do so. For a body to grow or for body functioning to be maintained, there must be a continual production of new cells. Our individual survivals are based on ...
WLC4 91-92
WLC4 91-92

...  If we use 1000 channels for the whole city with one Base station then obviously 1000 people can talk at the same time.  Then, how many people are actually speaking simultaneously with their phones in Tehran? ...
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Cell culture



Cell culture is the process by which cells are grown under controlled conditions, generally outside of their natural environment. In practice, the term ""cell culture"" now refers to the culturing of cells derived from multicellular eukaryotes, especially animal cells, in contrast with other types of culture that also grow cells, such as plant tissue culture, fungal culture, and microbiological culture (of microbes). The historical development and methods of cell culture are closely interrelated to those of tissue culture and organ culture. Viral culture is also related, with cells as hosts for the viruses. The laboratory technique of maintaining live cell lines (a population of cells descended from a single cell and containing the same genetic makeup) separated from their original tissue source became more robust in the middle 20th century.
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