Tns (H-300): sc-28542 - Santa Cruz Biotechnology
... Tensin (Tns) is an actin filament capping protein localized to various types of adherens junctions in muscle and non-muscle cells. Tensin is involved in the maintenance of cellular structure by anchoring actin filaments at the focal adhesion via F-Actin binding and capping activities. However, tensi ...
... Tensin (Tns) is an actin filament capping protein localized to various types of adherens junctions in muscle and non-muscle cells. Tensin is involved in the maintenance of cellular structure by anchoring actin filaments at the focal adhesion via F-Actin binding and capping activities. However, tensi ...
Molecular cloning, sequence analysis, and function of the intestinal
... with pcDNA3.1, which was cut with the same enzymes. The ligation product was transferred into DH5α, which was spread on an antiampicillin LB plate and cultured at 37°C for 12–16 h. Several clones were then chosen from the plate, and their plasmids were extracted for PCR and enzyme digestion and sequ ...
... with pcDNA3.1, which was cut with the same enzymes. The ligation product was transferred into DH5α, which was spread on an antiampicillin LB plate and cultured at 37°C for 12–16 h. Several clones were then chosen from the plate, and their plasmids were extracted for PCR and enzyme digestion and sequ ...
What is the endocrine system?
... Not all cells can make hormones. Only cells that are part of the endocrine gland or endocrine tissue can secrete or give off hormones. A gland is a structure that secretes hormones or other substances. There are two main kinds of glands: endocrine glands and exocrine glands. Endocrine glands are duc ...
... Not all cells can make hormones. Only cells that are part of the endocrine gland or endocrine tissue can secrete or give off hormones. A gland is a structure that secretes hormones or other substances. There are two main kinds of glands: endocrine glands and exocrine glands. Endocrine glands are duc ...
MICROTUBULE ORGANIZATION 1 Regulates
... chromosomal arrangement (Fig. 4D). Other spindles had short microtubules but normally arranged chromosomes (Fig. 4E), whereas in other instances spindles were fragmented so that one or more pairs of chromosomes was separated from the others (Fig. 4F). Phragmoplasts were typically crooked and fragmen ...
... chromosomal arrangement (Fig. 4D). Other spindles had short microtubules but normally arranged chromosomes (Fig. 4E), whereas in other instances spindles were fragmented so that one or more pairs of chromosomes was separated from the others (Fig. 4F). Phragmoplasts were typically crooked and fragmen ...
RtoA links initial cell type choice to the cell cycle in
... To examine the mechanisms of asymmetric cell division and cell-cycle-dependent cell-type choice, mutants with defects in these processes were identified in a shotgun antisense mutagenesis screen (Spann et al., 1996). Assuming that genes involved in these mechanisms are active at the time of starvati ...
... To examine the mechanisms of asymmetric cell division and cell-cycle-dependent cell-type choice, mutants with defects in these processes were identified in a shotgun antisense mutagenesis screen (Spann et al., 1996). Assuming that genes involved in these mechanisms are active at the time of starvati ...
PCM-1, A 228-kD Centrosome Autoantigen with a Distinct Cell Cycle
... For some experiments, trachea were isolated from recently sacrificed rats and used for the isolation of ciliated cells. Trachea were cut in half longitudinally and then the surfaces were scraped with a razor blade to dislodge epithelial cells. The cells were collected, rinsed twice with PBS, and the ...
... For some experiments, trachea were isolated from recently sacrificed rats and used for the isolation of ciliated cells. Trachea were cut in half longitudinally and then the surfaces were scraped with a razor blade to dislodge epithelial cells. The cells were collected, rinsed twice with PBS, and the ...
49 Localization of enzymes in certain secretory cells of Helix
... globules of Helix. However, in the spermatid of Helix, alkaline phosphatase and TPPase are localized in the Golgi lamellae; acid phosphatase is not present in the spermatids at all, although some is found in the nurse cells. These results indicate that the localization of each of these enzymes may d ...
... globules of Helix. However, in the spermatid of Helix, alkaline phosphatase and TPPase are localized in the Golgi lamellae; acid phosphatase is not present in the spermatids at all, although some is found in the nurse cells. These results indicate that the localization of each of these enzymes may d ...
New Insights of Transmembranal Mechanism and Subcellular
... and cancerous cells, as well as in macrophages, under normal physiological conditions. We also conducted mechanistic studies for the subcellular localization of SWNTs in cells. Through fluorescent labeling of all major subcellular components, we pinpointed the mitochondrial localization of SWNT-PL-P ...
... and cancerous cells, as well as in macrophages, under normal physiological conditions. We also conducted mechanistic studies for the subcellular localization of SWNTs in cells. Through fluorescent labeling of all major subcellular components, we pinpointed the mitochondrial localization of SWNT-PL-P ...
Bangor School Department Grade 7 Science
... E3a: Describe the basic functions of organisms carried out within cells including the extracting of energy from food and the elimination of wastes. E3c: Compare the structures, systems, and interactions that allow single-celled organisms and multi-celled plants and animals, including humans to defen ...
... E3a: Describe the basic functions of organisms carried out within cells including the extracting of energy from food and the elimination of wastes. E3c: Compare the structures, systems, and interactions that allow single-celled organisms and multi-celled plants and animals, including humans to defen ...
Growing and Working with Peripheral Neurons
... even to plastic culture dishes (except when methylcellulose is added to the medium; see later). There are several possibilities for treating these surfaces that enhance their capacity to adhere to cells. As with the rest of this chapter, we describe the most commonly used approaches in our laborator ...
... even to plastic culture dishes (except when methylcellulose is added to the medium; see later). There are several possibilities for treating these surfaces that enhance their capacity to adhere to cells. As with the rest of this chapter, we describe the most commonly used approaches in our laborator ...
Cells in Vitro - Cancer Research
... tion of vitamins, antibiotics, salts, glucose, and chilled in a refrigerator for several hours to free the dialyzed human serum was the same as that re cells from the glass. Cell counts showed that the ported for Medium 2 (11), except that inositol was cultures had progressed uniformly within each r ...
... tion of vitamins, antibiotics, salts, glucose, and chilled in a refrigerator for several hours to free the dialyzed human serum was the same as that re cells from the glass. Cell counts showed that the ported for Medium 2 (11), except that inositol was cultures had progressed uniformly within each r ...
Beyond apoptosis: nonapoptotic cell death in physiology and disease
... Additional evidence is available to support the idea that alternative signaling pathways triggered by Fas can lead to necrosis-like PCD. A caspase 8-deficient subline of human Jurkat cells can be killed by the enforced oligomerization of FADD under artificial conditions. Interestingly, the cell deat ...
... Additional evidence is available to support the idea that alternative signaling pathways triggered by Fas can lead to necrosis-like PCD. A caspase 8-deficient subline of human Jurkat cells can be killed by the enforced oligomerization of FADD under artificial conditions. Interestingly, the cell deat ...
Introduction to histopathology
... immune response and the target organ. However a characteristic of many organspecific diseases is that autoantibodies bind to the antigen within the tissue and recruit inflammatory cells. In this case, direct immunofluorescence microscopy can be used to identify the presence of antibodies, which goes ...
... immune response and the target organ. However a characteristic of many organspecific diseases is that autoantibodies bind to the antigen within the tissue and recruit inflammatory cells. In this case, direct immunofluorescence microscopy can be used to identify the presence of antibodies, which goes ...
In vitro control of neuronal polarity by
... 1.5 fig ml" 1 polyornithine were cultured for 3 days, fixed for 1 hour at 4°C with paraformaldehyde (4% in PBS), rinsed in PBS-glycine 5 mM pH 7.4, incubated (1 hour 37°C) with antiMAP2 antibodies (1/400) in PBS plus 10% FCS and 0.01% saponin (buffer A), washed 3 times and further incubated for 1 ho ...
... 1.5 fig ml" 1 polyornithine were cultured for 3 days, fixed for 1 hour at 4°C with paraformaldehyde (4% in PBS), rinsed in PBS-glycine 5 mM pH 7.4, incubated (1 hour 37°C) with antiMAP2 antibodies (1/400) in PBS plus 10% FCS and 0.01% saponin (buffer A), washed 3 times and further incubated for 1 ho ...
Brown and Goldstein: The Cholesterol Chronicles
... the presence of LDL in the culture medium. In striking contrast, no such suppression occurred in skin cells from the homozygous FH patients. Next, Brown and Goldstein set out to find whether skin cells of homozygous FH patients could bind LDL, as did the cells of healthy people. (Although the liver i ...
... the presence of LDL in the culture medium. In striking contrast, no such suppression occurred in skin cells from the homozygous FH patients. Next, Brown and Goldstein set out to find whether skin cells of homozygous FH patients could bind LDL, as did the cells of healthy people. (Although the liver i ...
Immunoglobulin detection
... the administration of antigen. After this, a sharp drop in the number of circulating ASC occurs 4. Memory B cells, on the other hand, need to be stimulated before they produce detectable amounts of antibody. This is typically done in a pre-incubation step where the cells are cultured in the presence ...
... the administration of antigen. After this, a sharp drop in the number of circulating ASC occurs 4. Memory B cells, on the other hand, need to be stimulated before they produce detectable amounts of antibody. This is typically done in a pre-incubation step where the cells are cultured in the presence ...
What are the basic functions of microfilaments? Insights from studies
... During the last few years, a large number of genes encoding proteins important for microfilament organization and function, many related to those in animal cells, have been identified in yeast. Analysis of the functions of these proteins is still in its infancy, but roles for microfilaments in vesic ...
... During the last few years, a large number of genes encoding proteins important for microfilament organization and function, many related to those in animal cells, have been identified in yeast. Analysis of the functions of these proteins is still in its infancy, but roles for microfilaments in vesic ...
The Benzophenanthridine Alkaloid Fagaronine Induces
... growth phase. Cell cultures were incubated in a 5 % CO2 humidified atmosphere at 37 8C during 72 hours. Growth inhibition and cell viability were evaluated as previously described [3]. After 3 days of treatment, the number of erythroid differentiated cells was determined by scoring benzidine-positiv ...
... growth phase. Cell cultures were incubated in a 5 % CO2 humidified atmosphere at 37 8C during 72 hours. Growth inhibition and cell viability were evaluated as previously described [3]. After 3 days of treatment, the number of erythroid differentiated cells was determined by scoring benzidine-positiv ...
Visualizing the actin cytoskeleton in living plant cells using a photo
... similar photo-convertible proteins. For example, in situations where EosFP or similar proteins are being used to monitor organelle fusion a partial conversion can easily create a yellow colour. This can be misinterpreted as accumulation of green and red fluorescent species within an organelle and su ...
... similar photo-convertible proteins. For example, in situations where EosFP or similar proteins are being used to monitor organelle fusion a partial conversion can easily create a yellow colour. This can be misinterpreted as accumulation of green and red fluorescent species within an organelle and su ...
A novel role for 14–3–3s in regulating epithelial
... hepatocyte growth factor (HGF) stimulation, the 14–3–3s knockdown epithelial cells also exhibited an eightfold reduction in formation of polarized epithelial structures, and the structures they formed were more depolarized when compared with the controls (Fig. 3B,C). On the other hand, under the tre ...
... hepatocyte growth factor (HGF) stimulation, the 14–3–3s knockdown epithelial cells also exhibited an eightfold reduction in formation of polarized epithelial structures, and the structures they formed were more depolarized when compared with the controls (Fig. 3B,C). On the other hand, under the tre ...
True or False Questions - TDSB School Web Site List
... 3.these carbohydrate and protein combinations known as phospholipids.___ 4.the phospholipid bilayer is composed of two rows or layers of pholipid molecules.___ 5.composed of cell walls with the compound lignin attached, is as strong as it is.____ 6. the molecules are placed randomly and there is on ...
... 3.these carbohydrate and protein combinations known as phospholipids.___ 4.the phospholipid bilayer is composed of two rows or layers of pholipid molecules.___ 5.composed of cell walls with the compound lignin attached, is as strong as it is.____ 6. the molecules are placed randomly and there is on ...
Supporting online material for Elowitz et al. report
... For further repression of reporter genes (such as in Fig. 3, B and C), plasmid pREP4 (Qiagen) was introduced into cells. pREP4 carries the lacIq promoter mutation for high-level expression of lac repressor. For experiments with the Repressilator, we replaced the ampR gene on the original plasmid (S5 ...
... For further repression of reporter genes (such as in Fig. 3, B and C), plasmid pREP4 (Qiagen) was introduced into cells. pREP4 carries the lacIq promoter mutation for high-level expression of lac repressor. For experiments with the Repressilator, we replaced the ampR gene on the original plasmid (S5 ...
The Antiglobulin Test
... The electrical potential between the red cell surface and the outer ionic cloud surrounding it is the zeta potential. ...
... The electrical potential between the red cell surface and the outer ionic cloud surrounding it is the zeta potential. ...
Cell culture
Cell culture is the process by which cells are grown under controlled conditions, generally outside of their natural environment. In practice, the term ""cell culture"" now refers to the culturing of cells derived from multicellular eukaryotes, especially animal cells, in contrast with other types of culture that also grow cells, such as plant tissue culture, fungal culture, and microbiological culture (of microbes). The historical development and methods of cell culture are closely interrelated to those of tissue culture and organ culture. Viral culture is also related, with cells as hosts for the viruses. The laboratory technique of maintaining live cell lines (a population of cells descended from a single cell and containing the same genetic makeup) separated from their original tissue source became more robust in the middle 20th century.