Replication of Marburg Virus in Human Endothelial Cells
... cells: Dulbecco medium containing 2% fetal calf serum (FCS); HUVEC: Medium 199 containing 20% human serum). Infected cells were incubated for the appropriate time at 370C ( 14, 15). Plaque assay. Confluent monolayers of E6 cells (cultured in six well tissue culture plates, Greiner, Frickenhausen, Ge ...
... cells: Dulbecco medium containing 2% fetal calf serum (FCS); HUVEC: Medium 199 containing 20% human serum). Infected cells were incubated for the appropriate time at 370C ( 14, 15). Plaque assay. Confluent monolayers of E6 cells (cultured in six well tissue culture plates, Greiner, Frickenhausen, Ge ...
Inheritance of biological information
... all within a group of species that are so similar to one another that most people cannot tell them apart.7 The chromosomes have been grossly scrambled, yet the cell is still able to extract the information it needs for survival. If the genes had been in control during such a scrambling process, Darw ...
... all within a group of species that are so similar to one another that most people cannot tell them apart.7 The chromosomes have been grossly scrambled, yet the cell is still able to extract the information it needs for survival. If the genes had been in control during such a scrambling process, Darw ...
The endoplasmic reticulum exerts control over organelle streaming
... streaming was negatively affected in the RHD3 mutants compared with the wild type (Fig. 4), especially at later stages of cell expansion when the presence of RHD3 has a major role in ER streaming (Fig. 1B). These data support that general organelle streaming is not constant during the growth of vege ...
... streaming was negatively affected in the RHD3 mutants compared with the wild type (Fig. 4), especially at later stages of cell expansion when the presence of RHD3 has a major role in ER streaming (Fig. 1B). These data support that general organelle streaming is not constant during the growth of vege ...
Chapter 5 Section 1 Passive Transport
... are not soluble in lipids or are too large (e.g. glucose) to pass through pores in membrane • In facilitated diffusion, a molecule binds to a carrier protein on one side of the cell membrane. • The carrier protein (specific for one type of molecule) then changes its shape and transports the molecule ...
... are not soluble in lipids or are too large (e.g. glucose) to pass through pores in membrane • In facilitated diffusion, a molecule binds to a carrier protein on one side of the cell membrane. • The carrier protein (specific for one type of molecule) then changes its shape and transports the molecule ...
to get the file - Chair of Computational Biology
... In the limiting case, where all constraints on the metabolic network are known, such as the enzyme kinetics and gene regulation, the feasible set may be reduced to a single point. This single point must lie within the feasible set. 15. Lecture WS 2012/13 ...
... In the limiting case, where all constraints on the metabolic network are known, such as the enzyme kinetics and gene regulation, the feasible set may be reduced to a single point. This single point must lie within the feasible set. 15. Lecture WS 2012/13 ...
A Tour of the Cell
... The presence of a central vacuole shifts the more active cytoplasmic areas closer to the plasma membrane, which, being then at a lower distance from the outer member, places less of a burden on the outer cell surface area. ...
... The presence of a central vacuole shifts the more active cytoplasmic areas closer to the plasma membrane, which, being then at a lower distance from the outer member, places less of a burden on the outer cell surface area. ...
Chapter 6 A Tour of the Cell
... C) They are built de novo from cytosol materials. D) They split in two after they are too large. E) The cell synthesizes hydrogen peroxide and encloses it in a membrane. Answer: D Topic: Concept 6.5 Skill: Knowledge/Comprehension ...
... C) They are built de novo from cytosol materials. D) They split in two after they are too large. E) The cell synthesizes hydrogen peroxide and encloses it in a membrane. Answer: D Topic: Concept 6.5 Skill: Knowledge/Comprehension ...
Histological and ultrastructural analysis of the
... (Soares et al., 2014), enabling the development of new varieties more adapted to each environment. Particularly, tissue culture has contributed greatly to plant regeneration of Banana through clonal propagation and micropropagation. Moreover, among the tissue culture techniques the somatic embryogen ...
... (Soares et al., 2014), enabling the development of new varieties more adapted to each environment. Particularly, tissue culture has contributed greatly to plant regeneration of Banana through clonal propagation and micropropagation. Moreover, among the tissue culture techniques the somatic embryogen ...
Origin and shaping of the laterality organ in zebrafish
... Fig. 2. Apical attachment of the dorsal forerunner cell cluster to the enveloping layer and subsequent detachment to form a cellular rosette during gastrulation. (A-B⬘) Confocal images of Tg(β-actin:HRASEGFP) zebrafish embryos immunolabelled with an antiaPKC-ζ antibody. GFP (green) and aPKC-ζ (red) ...
... Fig. 2. Apical attachment of the dorsal forerunner cell cluster to the enveloping layer and subsequent detachment to form a cellular rosette during gastrulation. (A-B⬘) Confocal images of Tg(β-actin:HRASEGFP) zebrafish embryos immunolabelled with an antiaPKC-ζ antibody. GFP (green) and aPKC-ζ (red) ...
Cell Proliferation Kinetics and Drug Sensitivity of
... The 1st possibility was depletion of essential nutrients. When medium containing stationary cells was adjusted to pH 7.2 and then enriched by adding amino acids, vitamins, glucose, or fetal calf serum, growth still ceased at about 2 x 106/ml. The 2nd possible reason was production of toxic environme ...
... The 1st possibility was depletion of essential nutrients. When medium containing stationary cells was adjusted to pH 7.2 and then enriched by adding amino acids, vitamins, glucose, or fetal calf serum, growth still ceased at about 2 x 106/ml. The 2nd possible reason was production of toxic environme ...
The cancer stem cell: premises, promises and challenges
... provided a ready explanation for the relentless advance toward ever cancer cells, and the two fractions showed equal cell cycle kinetics24, more malignant behavior within established tumors. yet the tumors seemed hierarchically organized when tested functionally. This paper was rapidly followed by s ...
... provided a ready explanation for the relentless advance toward ever cancer cells, and the two fractions showed equal cell cycle kinetics24, more malignant behavior within established tumors. yet the tumors seemed hierarchically organized when tested functionally. This paper was rapidly followed by s ...
1. Introduction 2. Fundamentals 3. Glycosylation 4
... Glycosylation of therapeutic proteins As mentioned earlier, several hundred proteins have been expressed recombinanly in the last couple of years in yeast. By now, the host system wasn’t able to do some of the most critical modifications so a lot of highly interesting proteins such as some antibodie ...
... Glycosylation of therapeutic proteins As mentioned earlier, several hundred proteins have been expressed recombinanly in the last couple of years in yeast. By now, the host system wasn’t able to do some of the most critical modifications so a lot of highly interesting proteins such as some antibodie ...
NNF1 is an essential yeast gene required for proper spindle
... for nuclear envelope proteins. The antisera recognized several proteins by immunoblot analysis and was used to screen a yeast λgt11 expression library. Several positive clones were isolated, including one that contained part of an ORF with 201 amino acids (Fig. 1). We named this gene NNF1 (necessary ...
... for nuclear envelope proteins. The antisera recognized several proteins by immunoblot analysis and was used to screen a yeast λgt11 expression library. Several positive clones were isolated, including one that contained part of an ORF with 201 amino acids (Fig. 1). We named this gene NNF1 (necessary ...
The Physiology of Gibberellin-Induced Elongation
... growth in response to GA treatment is not accompanied by proton secretion. These data contrast with the observations of Hebard et al. (25), who have shown that acid production accompanies GA treatment of Avena stem segments. If protons are not responsible for changing the tensile properties of the c ...
... growth in response to GA treatment is not accompanied by proton secretion. These data contrast with the observations of Hebard et al. (25), who have shown that acid production accompanies GA treatment of Avena stem segments. If protons are not responsible for changing the tensile properties of the c ...
Role of reactive oxygen species in cell signalling pathways Abstract
... catalyses the formation of hypochlorous acid (HOCl), while superoxide may also react with nitric oxide (NOd) to form another relatively ...
... catalyses the formation of hypochlorous acid (HOCl), while superoxide may also react with nitric oxide (NOd) to form another relatively ...
AP Biology - TeacherWeb
... _____________________ loosely bound to surface of membrane ex: cell surface identity marker (________) ...
... _____________________ loosely bound to surface of membrane ex: cell surface identity marker (________) ...
Zygotic expression of the pebble locus is required for cytokinesis
... growth of membranes from the periphery of the embryo to enclose the nuclei, resulting in the formation of the cellular blastoderm. The 14th mitoses are radically different from all preceding mitoses. They are the first cellular mitoses and they occur asynchronously, groups of cells dividing in a com ...
... growth of membranes from the periphery of the embryo to enclose the nuclei, resulting in the formation of the cellular blastoderm. The 14th mitoses are radically different from all preceding mitoses. They are the first cellular mitoses and they occur asynchronously, groups of cells dividing in a com ...
Measuring Electrical and Mechanical Properties of Red Blood Cells
... is divided once and recombined using polarizer beam-splitters (BP1 and BP2), as shown in Figure 1. There is a halfwave plate (H1) before the first beam-splitter to distribute the power for each beam. Before the second beam-splitter there is another half-wave plate (H2) to control the power of one of ...
... is divided once and recombined using polarizer beam-splitters (BP1 and BP2), as shown in Figure 1. There is a halfwave plate (H1) before the first beam-splitter to distribute the power for each beam. Before the second beam-splitter there is another half-wave plate (H2) to control the power of one of ...
Intra-epithelial mast cells in human airway epithelium
... levels demonstrates many of the typical features of mast cells-namely, metachromatic granules on staining with toluidine blue at light microscopic level, surface microvillous processes, and numerous dense intracytoplasmic granules with a whorled substructure at electron microscopy. Furthermore, we h ...
... levels demonstrates many of the typical features of mast cells-namely, metachromatic granules on staining with toluidine blue at light microscopic level, surface microvillous processes, and numerous dense intracytoplasmic granules with a whorled substructure at electron microscopy. Furthermore, we h ...
Shroom3-mediated recruitment of Rho kinases to the apical cell
... were incubated with the appropriate antibodies at 4°C for 2 hours. Then protein G-Sepharose beads (GE Healthcare) were added and incubation was continued for an additional 30 minutes. After washing the beads, bound proteins were subjected to SDS-PAGE and then western blotting. Signals on the bots we ...
... were incubated with the appropriate antibodies at 4°C for 2 hours. Then protein G-Sepharose beads (GE Healthcare) were added and incubation was continued for an additional 30 minutes. After washing the beads, bound proteins were subjected to SDS-PAGE and then western blotting. Signals on the bots we ...
Slide 1
... An unit cell unit of the structure [is a line segment in 1D, a parallelogram in 2D and a parallelepiped in 3D], such that lattice points are at the ends of the line segment (1D) and at the vertices of the parallelogram (2D) or parallelepiped (3D); which when repeated by the translational symmetry ...
... An unit cell unit of the structure [is a line segment in 1D, a parallelogram in 2D and a parallelepiped in 3D], such that lattice points are at the ends of the line segment (1D) and at the vertices of the parallelogram (2D) or parallelepiped (3D); which when repeated by the translational symmetry ...
Shroom3-mediated recruitment of Rho kinases to the apical cell
... were incubated with the appropriate antibodies at 4°C for 2 hours. Then protein G-Sepharose beads (GE Healthcare) were added and incubation was continued for an additional 30 minutes. After washing the beads, bound proteins were subjected to SDS-PAGE and then western blotting. Signals on the bots we ...
... were incubated with the appropriate antibodies at 4°C for 2 hours. Then protein G-Sepharose beads (GE Healthcare) were added and incubation was continued for an additional 30 minutes. After washing the beads, bound proteins were subjected to SDS-PAGE and then western blotting. Signals on the bots we ...
Cell culture
Cell culture is the process by which cells are grown under controlled conditions, generally outside of their natural environment. In practice, the term ""cell culture"" now refers to the culturing of cells derived from multicellular eukaryotes, especially animal cells, in contrast with other types of culture that also grow cells, such as plant tissue culture, fungal culture, and microbiological culture (of microbes). The historical development and methods of cell culture are closely interrelated to those of tissue culture and organ culture. Viral culture is also related, with cells as hosts for the viruses. The laboratory technique of maintaining live cell lines (a population of cells descended from a single cell and containing the same genetic makeup) separated from their original tissue source became more robust in the middle 20th century.