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... Na2PO4, 2 MgCl2, 2 CaCl2, 26 NaHCO3, and 10 glucose and for rat slices contained: 125 NaCl, 3 KCl, 1.25 NaH2PO4, 1 MgCl2, 2 CaCl2, 25 NaHCO3, and 25 glucose. Recording pipettes were pulled from borosilicate glass capillaries and had tip resistances of 4 – 8 M⍀ when filled with an intracellular solut ...
... Na2PO4, 2 MgCl2, 2 CaCl2, 26 NaHCO3, and 10 glucose and for rat slices contained: 125 NaCl, 3 KCl, 1.25 NaH2PO4, 1 MgCl2, 2 CaCl2, 25 NaHCO3, and 25 glucose. Recording pipettes were pulled from borosilicate glass capillaries and had tip resistances of 4 – 8 M⍀ when filled with an intracellular solut ...
Cancer stem cells - normal stem cells "Jedi" that went over to the
... lignant transformation of dormant embryonic stem celllike cells corresponds very well with the development of some rare tumors (e.g., teratomas) that usually are encountered in younger patients [41]. Nowadays, however, it is postulated that normal TCSC/PSC are the equivalent of Virchow’s embryonic r ...
... lignant transformation of dormant embryonic stem celllike cells corresponds very well with the development of some rare tumors (e.g., teratomas) that usually are encountered in younger patients [41]. Nowadays, however, it is postulated that normal TCSC/PSC are the equivalent of Virchow’s embryonic r ...
Through the Cell Membrane
... environment vastly different from that outside the cell. For example, it must concentrate nutrients for growth and maintenance inside, and also carry out any specialized functions it may have. In addition, many of the cell’s waste products are highly toxic and must be completely removed from the int ...
... environment vastly different from that outside the cell. For example, it must concentrate nutrients for growth and maintenance inside, and also carry out any specialized functions it may have. In addition, many of the cell’s waste products are highly toxic and must be completely removed from the int ...
Proposal - people.vcu.edu
... epidermal cells during prenatal development. Whittock et al (2002) previously conducted a study in which an individual displaying a skin abnormality was observed from birth into adulthood. The patient was found to have a mutation in desmoplakin, ultimately leading to blistering and an early onset of ...
... epidermal cells during prenatal development. Whittock et al (2002) previously conducted a study in which an individual displaying a skin abnormality was observed from birth into adulthood. The patient was found to have a mutation in desmoplakin, ultimately leading to blistering and an early onset of ...
Fluorescent tagging of an endogenous gene by
... of DSBs using the crRNA-guided Cas9 nuclease. For HDR experiments, selection of the crRNA target site is a balance between the efficiency of the DSB caused by the Cas9 nuclease and the distance of the DSB from the desired HDR insertion/alteration site. It has been reported that mammalian gene conver ...
... of DSBs using the crRNA-guided Cas9 nuclease. For HDR experiments, selection of the crRNA target site is a balance between the efficiency of the DSB caused by the Cas9 nuclease and the distance of the DSB from the desired HDR insertion/alteration site. It has been reported that mammalian gene conver ...
The CENP-O complex requirement varies among different cell types
... died during early embryogenesis. To examine CENP-U requirements for viability at the ES cell level, we generated conditional CENP-U-deficient mouse ES cells by a gene targeting approach. The strategy used to generate conditional CENP-U-deficient ES cells is shown in Fig. 2a, b. By sequential gene ta ...
... died during early embryogenesis. To examine CENP-U requirements for viability at the ES cell level, we generated conditional CENP-U-deficient mouse ES cells by a gene targeting approach. The strategy used to generate conditional CENP-U-deficient ES cells is shown in Fig. 2a, b. By sequential gene ta ...
Dendritic Cell Development in Long
... apart from cytokines combine to drive progenitor cells to differentiate into DCs. While growth factors like Flt3L and GMCSF can support DC development both in vivo and in vitro, these cytokines do not have an essential or even a specific role in lineage commitment [15, 16]. The study of progenitors ...
... apart from cytokines combine to drive progenitor cells to differentiate into DCs. While growth factors like Flt3L and GMCSF can support DC development both in vivo and in vitro, these cytokines do not have an essential or even a specific role in lineage commitment [15, 16]. The study of progenitors ...
ANIMAL KINGDOM 1 EVOLUTIONARY TRENDS and PHYLUM
... unattached end frayed out into several strands. Because of its small size, transparency and habit of contracting down into a little ball when disturbed, the animal is readily overlooked. Yet, hydras are abundant and are the only really successful ones among the few members of their phylum that have ...
... unattached end frayed out into several strands. Because of its small size, transparency and habit of contracting down into a little ball when disturbed, the animal is readily overlooked. Yet, hydras are abundant and are the only really successful ones among the few members of their phylum that have ...
Role of Membrane Potential in the Regulation of Cell Proliferation
... Resting potentials of various cell types fall within a wide range (generally −10 mV to −90 mV), and cells’ positions along such a Vmem scale generally correspond to their proliferative potential [24]. Somatic cells that have a high degree of polarization (a hyperpolarized Vmem) tend to be quiescent ...
... Resting potentials of various cell types fall within a wide range (generally −10 mV to −90 mV), and cells’ positions along such a Vmem scale generally correspond to their proliferative potential [24]. Somatic cells that have a high degree of polarization (a hyperpolarized Vmem) tend to be quiescent ...
Cells - MCQ topic quiz
... We’d like to know your view on the resources we produce. By clicking on ‘Like’ or ‘Dislike’ you can help us to ensure that our resources work for you. When the email template pops up please add additional comments if you wish and then just click ‘Send’. Thank you. If you do not currently offer this ...
... We’d like to know your view on the resources we produce. By clicking on ‘Like’ or ‘Dislike’ you can help us to ensure that our resources work for you. When the email template pops up please add additional comments if you wish and then just click ‘Send’. Thank you. If you do not currently offer this ...
How much territory can a single E. coli cell control?
... enclose a large enough volume of cytoplasm to present it with major transport problems. This review surveys E. coli cells much longer than those which grow in nature and in usual lab cultures. These include cells mutated in a single gene (metK) which are 2–4× longer than their non-mutated parent. Th ...
... enclose a large enough volume of cytoplasm to present it with major transport problems. This review surveys E. coli cells much longer than those which grow in nature and in usual lab cultures. These include cells mutated in a single gene (metK) which are 2–4× longer than their non-mutated parent. Th ...
Platelet Role in Leukocyte Extravasation
... actually leave the blood vessels, and what role platelets play in both of these processes. The scientists hypothesized that platelets "guide leukocytes to their site of extravasation" by helping them find endothelial cell junctions (Zuchtriegel, 2016). Because inflammation plays a role in many diffe ...
... actually leave the blood vessels, and what role platelets play in both of these processes. The scientists hypothesized that platelets "guide leukocytes to their site of extravasation" by helping them find endothelial cell junctions (Zuchtriegel, 2016). Because inflammation plays a role in many diffe ...
Flat file for auto loading into the CBO 1.0 version. Individual sections
... An account of the resource. Description may include but is not limited to: an abstract, a table of contents, a graphical representation, or a free-text account of the resource. An entity primarily responsible for making the resource. A point or period of time associated with an event in the lifecycl ...
... An account of the resource. Description may include but is not limited to: an abstract, a table of contents, a graphical representation, or a free-text account of the resource. An entity primarily responsible for making the resource. A point or period of time associated with an event in the lifecycl ...
The Wall Becomes Surmountable
... cell wall in response to various types of stresses. Although 1,3--glucan synthases have proven to be recalcitrant to study by conventional biochemistry, Geoffrey V. Fincher (University of Adelaide, Australia) described recent successes that his laboratory has had in characterizing these enzymes by ...
... cell wall in response to various types of stresses. Although 1,3--glucan synthases have proven to be recalcitrant to study by conventional biochemistry, Geoffrey V. Fincher (University of Adelaide, Australia) described recent successes that his laboratory has had in characterizing these enzymes by ...
Memories M. Carrie Miceli May 17, 2002
... – stable pool of memory cells can persist for many years. – accelerated T cell responses seen upon re-exposure to antigen due to: • Increased frequency (5-100 fold increase) • Qualitative changes in memory T cells that allow them to respond faster and develop into effector cells more efficiently tha ...
... – stable pool of memory cells can persist for many years. – accelerated T cell responses seen upon re-exposure to antigen due to: • Increased frequency (5-100 fold increase) • Qualitative changes in memory T cells that allow them to respond faster and develop into effector cells more efficiently tha ...
1-Functional Organization of the Human Body
... (especially hormones) into bloodstream (e.g. pituitary gland, pancreas secretes insulin into the blood ...
... (especially hormones) into bloodstream (e.g. pituitary gland, pancreas secretes insulin into the blood ...
Involvement of the cytoskeleton in Junin virus multiplication
... The distribution of actin-containing microfilaments was examined by using a monoclonal antibody to actin or FITC–phalloidin. Although microfilaments were not resolved efficiently under either conditions in Vero cells, the typical bundles of parallel stress fibres spanning the long axis of the cell w ...
... The distribution of actin-containing microfilaments was examined by using a monoclonal antibody to actin or FITC–phalloidin. Although microfilaments were not resolved efficiently under either conditions in Vero cells, the typical bundles of parallel stress fibres spanning the long axis of the cell w ...
Ultrastructural Evidences for Endothelial Cell - Leukocytes
... marginate on the acceptor molecules expressed by endothelium and extravasate in response to a chemotactic stimulus (Colditz, 1985). During lymphocyte recirculation high endothelial venules represent a primary site for lymphocyte migration to lymph nodes, tonsils mucosa associated lymphoid tissues an ...
... marginate on the acceptor molecules expressed by endothelium and extravasate in response to a chemotactic stimulus (Colditz, 1985). During lymphocyte recirculation high endothelial venules represent a primary site for lymphocyte migration to lymph nodes, tonsils mucosa associated lymphoid tissues an ...
Interaction of oxygen-sensitive luminescent probes Ru(phen) and
... crystals of LiPc. The spatial resolution of EPR oxygen images obtained in phantoms and tissues is too low for imaging individual cells [4]. NMR approaches appear to be non-invasive but also offer limited resolution [7]. In contrast, approaches based on the use of luminescent probes like ruthenium(II ...
... crystals of LiPc. The spatial resolution of EPR oxygen images obtained in phantoms and tissues is too low for imaging individual cells [4]. NMR approaches appear to be non-invasive but also offer limited resolution [7]. In contrast, approaches based on the use of luminescent probes like ruthenium(II ...
The DNA Damage Response Signaling Cascade
... components to dikaryon formation varies among species characterized thus far, a central element common to all of them is the activation of a specific transcriptional cascade controlled by a heterodimeric homeodomain transcription factor, with components derived from the MAT locus of each parent. Dik ...
... components to dikaryon formation varies among species characterized thus far, a central element common to all of them is the activation of a specific transcriptional cascade controlled by a heterodimeric homeodomain transcription factor, with components derived from the MAT locus of each parent. Dik ...
Chapter 8: Cellular Transport and the Cell Cycle
... Cells in a hypotonic solution In the hypotonic solution in Figure 8.3A, the concentration of dissolved substances is lower in the solution outside the cell than the concentration inside the cell. Therefore, there is more water outside the cell than inside. Cells in a hypotonic solution experience os ...
... Cells in a hypotonic solution In the hypotonic solution in Figure 8.3A, the concentration of dissolved substances is lower in the solution outside the cell than the concentration inside the cell. Therefore, there is more water outside the cell than inside. Cells in a hypotonic solution experience os ...
DHY111 - Wayne County Community College District
... Upon completion of this course, the student will be familiar with: • Name the four basic tissues in the body and give an example of where each type is found. • List and give the relative size of various subdivisions of the meter down to the angstrom and give the range of human cell sizes. • Give the ...
... Upon completion of this course, the student will be familiar with: • Name the four basic tissues in the body and give an example of where each type is found. • List and give the relative size of various subdivisions of the meter down to the angstrom and give the range of human cell sizes. • Give the ...
Mission Debriefing for a science class and bacteria
... or RNA) surrounded by a protein coat called a capsid. Some viruses have a structure outside of capsid called an envelope that is formed from the membrane of the cell the virus infects. Method of Reproduction Bacteria and viruses differ in their method of reproduction in one very important way. Bacte ...
... or RNA) surrounded by a protein coat called a capsid. Some viruses have a structure outside of capsid called an envelope that is formed from the membrane of the cell the virus infects. Method of Reproduction Bacteria and viruses differ in their method of reproduction in one very important way. Bacte ...
Gene Section PLA2G2A (phospholipase A2, group IIA (platelets, synovial fluid))
... tract, COX-2 is best known for its promotion of inflammation, proliferation of cancer cells and resistance to apoptosis. Although the mechanisms through which sPLA2s stimulate COX-2 induction have not been completely resolved, sPLA2-mediated generation of lysophosphatidylcholine (lyso-PC) may play a ...
... tract, COX-2 is best known for its promotion of inflammation, proliferation of cancer cells and resistance to apoptosis. Although the mechanisms through which sPLA2s stimulate COX-2 induction have not been completely resolved, sPLA2-mediated generation of lysophosphatidylcholine (lyso-PC) may play a ...
Cell culture
Cell culture is the process by which cells are grown under controlled conditions, generally outside of their natural environment. In practice, the term ""cell culture"" now refers to the culturing of cells derived from multicellular eukaryotes, especially animal cells, in contrast with other types of culture that also grow cells, such as plant tissue culture, fungal culture, and microbiological culture (of microbes). The historical development and methods of cell culture are closely interrelated to those of tissue culture and organ culture. Viral culture is also related, with cells as hosts for the viruses. The laboratory technique of maintaining live cell lines (a population of cells descended from a single cell and containing the same genetic makeup) separated from their original tissue source became more robust in the middle 20th century.