Circulating Human Prostate Cancer Cells from an
... human prostate cancer tumor fragments at orthotopic and ectopic sites to determine their ability to deliver viable malignant cells into the circulation. Using PC-3 prostate cancer cells expressing either green fluorescent protein (GFP) or red fluorescent protein (RFP), we demonstrated that viable ci ...
... human prostate cancer tumor fragments at orthotopic and ectopic sites to determine their ability to deliver viable malignant cells into the circulation. Using PC-3 prostate cancer cells expressing either green fluorescent protein (GFP) or red fluorescent protein (RFP), we demonstrated that viable ci ...
An indelible lineage marker for Xenopus using a
... Many important Xenopus transcription factors are strongly expressed in early development, but cease to be expressed a few hours later (Smith, 1993; Dawid, 1994). To determine the function of such genes, one important experimental route is to express them ectopically in a tissue where they are not no ...
... Many important Xenopus transcription factors are strongly expressed in early development, but cease to be expressed a few hours later (Smith, 1993; Dawid, 1994). To determine the function of such genes, one important experimental route is to express them ectopically in a tissue where they are not no ...
The added value of single-cell gene expression
... populations is the focus area of single-cell studies. Established cell types as well as previously unknown subpopulations have been identified and characterized in detail at single-cell level using qPCR [11–19]. Analysis at single-cell level eliminates many of the factors that confound gene expressi ...
... populations is the focus area of single-cell studies. Established cell types as well as previously unknown subpopulations have been identified and characterized in detail at single-cell level using qPCR [11–19]. Analysis at single-cell level eliminates many of the factors that confound gene expressi ...
A Heat Shows of Two Shock-resistant Mutant of Saccharomyces
... Downloaded from on June 18, 2017 ...
... Downloaded from on June 18, 2017 ...
The Arabidopsis Callose Synthase Gene GSL8 Is
... (Supplemental Table S1), which was gametophytic lethal (Töller et al., 2008; Huang et al., 2009), thus explaining why gsl10 homozygous mutants could not be isolated. To identify the possible functions of different GSL genes in sporophytic callose formation and in cytokinesis, we examined gross and ...
... (Supplemental Table S1), which was gametophytic lethal (Töller et al., 2008; Huang et al., 2009), thus explaining why gsl10 homozygous mutants could not be isolated. To identify the possible functions of different GSL genes in sporophytic callose formation and in cytokinesis, we examined gross and ...
Chapter 22: The Living Cell - Follow “Ironmtn.wordpress.com”
... 58. At the cellular and molecular level, in what key ways are plants and animals the same? In what essential ways are they different? How do these differences give the organism an advantage in its life strategies? Ans: Cellular respiration is the same; plants have chloroplasts, which allows them to ...
... 58. At the cellular and molecular level, in what key ways are plants and animals the same? In what essential ways are they different? How do these differences give the organism an advantage in its life strategies? Ans: Cellular respiration is the same; plants have chloroplasts, which allows them to ...
localization of the succinic dehydrogenase system
... the size of the T N F deposits (Figs. 3 and 4). Some dividing cells (Fig. 5) appeared to have more SDH activity when compared with non-dividing cells. I n rare instances T N F deposits were seen within the nuclear area (Figs. 6 and 9). In cases where the deposition of T N F was not heavy, it was pos ...
... the size of the T N F deposits (Figs. 3 and 4). Some dividing cells (Fig. 5) appeared to have more SDH activity when compared with non-dividing cells. I n rare instances T N F deposits were seen within the nuclear area (Figs. 6 and 9). In cases where the deposition of T N F was not heavy, it was pos ...
THE HEART
... laboratory, stem cells from the bone marrow are exposed to growth factors. Growth factors are proteins that help the stem cells grow and stay healthy until they are put back in the body. In the laboratory, the stem cells multiply and grow to become new heart cells. These new heart cells are then pla ...
... laboratory, stem cells from the bone marrow are exposed to growth factors. Growth factors are proteins that help the stem cells grow and stay healthy until they are put back in the body. In the laboratory, the stem cells multiply and grow to become new heart cells. These new heart cells are then pla ...
Full text in pdf format
... specimens, and less frequently in living cockles, after the highest mortality observed in 1991 and 1992. This mortality occurred only during the summer when the temperature was higher. Signs associated with RSS infection may represent the response to environmental factors, as hypothesized in relatio ...
... specimens, and less frequently in living cockles, after the highest mortality observed in 1991 and 1992. This mortality occurred only during the summer when the temperature was higher. Signs associated with RSS infection may represent the response to environmental factors, as hypothesized in relatio ...
Pluripotent Stem Cell Handbook
... reprogramming vectors because they carry viral elements that allow the prolonged and controlled expression of reprogramming factors, but they can be transfected into cells without the need for viral packaging. ...
... reprogramming vectors because they carry viral elements that allow the prolonged and controlled expression of reprogramming factors, but they can be transfected into cells without the need for viral packaging. ...
The role of desmoplakin during epidermal development
... as an important structural link to the cytoskeleton (Figure 3) Without it, the desmosome fails to function properly with the rest of the cell (Garrod & Chigdey, 2008). b) The use of Xenopus laevis in studying desmosome function during development Xenopus laevis, also known as the African clawed frog ...
... as an important structural link to the cytoskeleton (Figure 3) Without it, the desmosome fails to function properly with the rest of the cell (Garrod & Chigdey, 2008). b) The use of Xenopus laevis in studying desmosome function during development Xenopus laevis, also known as the African clawed frog ...
Chaotic expression dynamics implies pluripotency: when theory and
... progressively decreases the number of cell types they can potentially become. Pluripotent stem cells can differentiate into several types of cells, but terminally differentiated cells cannot differentiate any further. A fundamental problem in stem cell biology is the characterization of the differen ...
... progressively decreases the number of cell types they can potentially become. Pluripotent stem cells can differentiate into several types of cells, but terminally differentiated cells cannot differentiate any further. A fundamental problem in stem cell biology is the characterization of the differen ...
File
... Protein channels that assist the diffusion of substances through the cell membrane do so by facilitated diffusion. Facilitated diffusion works in two directions. As long as a molecule or ion fits into the channel, it is free to pass through in either direction. Each kind of molecule or ion diffuses ...
... Protein channels that assist the diffusion of substances through the cell membrane do so by facilitated diffusion. Facilitated diffusion works in two directions. As long as a molecule or ion fits into the channel, it is free to pass through in either direction. Each kind of molecule or ion diffuses ...
Viruses
... 5. Is the following sentence true or false? Each virus can enter only a few types of cells in a few specific species. ___________________________________ 6. Is the following sentence true or false? All viruses have the same shape. ...
... 5. Is the following sentence true or false? Each virus can enter only a few types of cells in a few specific species. ___________________________________ 6. Is the following sentence true or false? All viruses have the same shape. ...
Alteration of the Cytoplasmic Domain of the Membrane
... screening of a large number of clones by placing the antibody solution (5 ~tl) directly on top of the coverchip. Immunofluorescence of MDCK cells grown on filters was carried out by the technique of Fuller et al. (1984) with the following modifications: (a) 10 % new born calf serum was used in place ...
... screening of a large number of clones by placing the antibody solution (5 ~tl) directly on top of the coverchip. Immunofluorescence of MDCK cells grown on filters was carried out by the technique of Fuller et al. (1984) with the following modifications: (a) 10 % new born calf serum was used in place ...
Plant Cells Contain Two Functionally Distinct
... al., 1992) cells. The processed mature form of aleurain colocalized with vacuolar enzyme markers in cell fractionation experiments (Holwerda et al., 1992). The enzymes responsible for processing proaleurain in barley aleurone cells have an acidic pH optimum (Holwerda et al., 1990), and aleurain puri ...
... al., 1992) cells. The processed mature form of aleurain colocalized with vacuolar enzyme markers in cell fractionation experiments (Holwerda et al., 1992). The enzymes responsible for processing proaleurain in barley aleurone cells have an acidic pH optimum (Holwerda et al., 1990), and aleurain puri ...
Cellular and Antitumor Activity of a New Diethylene Glycol
... tubes had a central wavelength of 450 6 10 nm and a full width half maximum of 40 6 10 nm. Light intensity was measured before each experiment with a National Biological Corporation power meter (model NBM447C, Twinsburg, OH). A light intensity of 7.2 mW/cm2 was used, resulting in a total dose of 4 J ...
... tubes had a central wavelength of 450 6 10 nm and a full width half maximum of 40 6 10 nm. Light intensity was measured before each experiment with a National Biological Corporation power meter (model NBM447C, Twinsburg, OH). A light intensity of 7.2 mW/cm2 was used, resulting in a total dose of 4 J ...
Electron Microscopic Observations on the Excretion of Cell
... secretory process of the rabbit apocrine sweat glands (Kurosumi, 1962). This process has some similarity with the excretory activity of V. cholerae, as postulated in this study, but in detailed analysis many differences may again be detected, some of which could be due to the differences between the ...
... secretory process of the rabbit apocrine sweat glands (Kurosumi, 1962). This process has some similarity with the excretory activity of V. cholerae, as postulated in this study, but in detailed analysis many differences may again be detected, some of which could be due to the differences between the ...
Unit 2 - Cells and Systems Learning Pack (Science In Action 8
... or reinforce concepts, covered in the Alberta Science Curriculum, and included in the Grade 8 Science Final Exam in June. There are circumstances in which an entire unit may be missed and covering the concepts from that unit (for the final exam) can be difficult. This can happen for a number of reas ...
... or reinforce concepts, covered in the Alberta Science Curriculum, and included in the Grade 8 Science Final Exam in June. There are circumstances in which an entire unit may be missed and covering the concepts from that unit (for the final exam) can be difficult. This can happen for a number of reas ...
Exit from dormancy in microbial organisms
... presence of conditions that support microbial growth. Consistent with the information provided by this signal, peptidoglycan derived from growing cells is a much more effective inducer of germination than that derived from cells in extended periods (~24 h) of stationary phase15. This effect is remin ...
... presence of conditions that support microbial growth. Consistent with the information provided by this signal, peptidoglycan derived from growing cells is a much more effective inducer of germination than that derived from cells in extended periods (~24 h) of stationary phase15. This effect is remin ...
Tissues - Trisha Hanka`s VTI site
... • Thin and delicate and branched into complicated networks. • Form support for around other cellular organs like endocrine glands. • _____________________: • Composed primarily of _____________. • Are branched to form networks • Lack tensile strength of collagenous fibers. • Can stretch and contract ...
... • Thin and delicate and branched into complicated networks. • Form support for around other cellular organs like endocrine glands. • _____________________: • Composed primarily of _____________. • Are branched to form networks • Lack tensile strength of collagenous fibers. • Can stretch and contract ...
Cytoskeleton remodelling of confluent epithelial cells cultured on
... fate of eukaryotic cells to a much larger extent than initially expected. In particular, environmental cues such as those emanating from the substrate itself, like topography, elasticity or surface functionalization, govern a large number of cellular responses encompassing cell growth, gene expressi ...
... fate of eukaryotic cells to a much larger extent than initially expected. In particular, environmental cues such as those emanating from the substrate itself, like topography, elasticity or surface functionalization, govern a large number of cellular responses encompassing cell growth, gene expressi ...
EXP 501 Hallmark Pap
... dependent pathways in which Calcineurin (Cn) signals other factors that play a crucial role in hypertrophic response (2). Hormones are long-distance, long-acting communicators of the body. They usually have one or more of three different effects on a cell: 1) Alter membrane transport of the cell. 2) ...
... dependent pathways in which Calcineurin (Cn) signals other factors that play a crucial role in hypertrophic response (2). Hormones are long-distance, long-acting communicators of the body. They usually have one or more of three different effects on a cell: 1) Alter membrane transport of the cell. 2) ...
Expression of PSA-RP2, an alternatively spliced
... could be detected, despite high levels of PSA (Figure 1D). This was unexpected as PSA-RP2 retains the N-terminal signal peptide required for secretion of PSA. In addition, previous results (Heuze-Vourc’h et al., 2001) had demonstrated secretion of recombinant PSA-RP2 from Cos-7 cells. To resolve thi ...
... could be detected, despite high levels of PSA (Figure 1D). This was unexpected as PSA-RP2 retains the N-terminal signal peptide required for secretion of PSA. In addition, previous results (Heuze-Vourc’h et al., 2001) had demonstrated secretion of recombinant PSA-RP2 from Cos-7 cells. To resolve thi ...
Cell culture
Cell culture is the process by which cells are grown under controlled conditions, generally outside of their natural environment. In practice, the term ""cell culture"" now refers to the culturing of cells derived from multicellular eukaryotes, especially animal cells, in contrast with other types of culture that also grow cells, such as plant tissue culture, fungal culture, and microbiological culture (of microbes). The historical development and methods of cell culture are closely interrelated to those of tissue culture and organ culture. Viral culture is also related, with cells as hosts for the viruses. The laboratory technique of maintaining live cell lines (a population of cells descended from a single cell and containing the same genetic makeup) separated from their original tissue source became more robust in the middle 20th century.