Review Article Stem cells for the cell and molecular therapy of type 1
... some genes used to generate iPSCs are oncogenes such as MYC [54, 55]. Recently, additional techniques have been developed to exclude the tumorigenic gene MYC so as to avoid genetic modifications, by use of microribonucleic acids (mir-) [56, 57]. Furthermore, iPSCs have been generated from patients w ...
... some genes used to generate iPSCs are oncogenes such as MYC [54, 55]. Recently, additional techniques have been developed to exclude the tumorigenic gene MYC so as to avoid genetic modifications, by use of microribonucleic acids (mir-) [56, 57]. Furthermore, iPSCs have been generated from patients w ...
Stem cell transplantation in multiple sclerosis
... Mesenchymal stem cells definition Multipotential stromal precursor cells were first isolated from the bone marrow, as the common ancestors of mesenchymal tissues such as cartilage, fat, bone and other connective tissues [18], and commonly termed as mesenchymal stem cells (MSCs). Many other tissues h ...
... Mesenchymal stem cells definition Multipotential stromal precursor cells were first isolated from the bone marrow, as the common ancestors of mesenchymal tissues such as cartilage, fat, bone and other connective tissues [18], and commonly termed as mesenchymal stem cells (MSCs). Many other tissues h ...
Ring, helix, sphere and cylinder: the basic geometry of prokaryotic
... confers rigidity to this structure. These enzymes are penicillinbinding proteins (PBPs) and belong to the SxxK superfamily of serine proteases. Class A SxxK peptidases act independently, whereas class B associate with either glycosyl transferases or acyl transferases. This latter class also associat ...
... confers rigidity to this structure. These enzymes are penicillinbinding proteins (PBPs) and belong to the SxxK superfamily of serine proteases. Class A SxxK peptidases act independently, whereas class B associate with either glycosyl transferases or acyl transferases. This latter class also associat ...
Flies evolved small bodies and cells at high or fluctuating
... of 20.5°C. Thus, at no point in time could natural selection have favored genotypes that perform well at 20.5°C. Instead, populations could have adapted to being held at 25°C in some generations and at 16°C in other generations (hence, to the variance of temperature), as alleles conferring greater f ...
... of 20.5°C. Thus, at no point in time could natural selection have favored genotypes that perform well at 20.5°C. Instead, populations could have adapted to being held at 25°C in some generations and at 16°C in other generations (hence, to the variance of temperature), as alleles conferring greater f ...
Euglena Coloring
... the reservoir. Color the reservoir grey and the flagellum black. The Euglena is unique in that it is both heterotrophic (must consume food) and autotrophic (can make its own food). Chloroplasts within the euglena trap sunlight that is used for photosynthesis, and can be seen as several rod like stru ...
... the reservoir. Color the reservoir grey and the flagellum black. The Euglena is unique in that it is both heterotrophic (must consume food) and autotrophic (can make its own food). Chloroplasts within the euglena trap sunlight that is used for photosynthesis, and can be seen as several rod like stru ...
Tutorial 3: Cells and Organelles
... c. Carries out photosynthesis / where chlorophyll traps solar energy d. Maintain cell shape and protects from mechanical stress ...
... c. Carries out photosynthesis / where chlorophyll traps solar energy d. Maintain cell shape and protects from mechanical stress ...
Cell Biology - WEB . WHRSD . ORG
... kidneys and the liver are closely associated with the circulatory system as they perform the excretory function of removing waste from the blood. Recognize that kidneys remove nitrogenous wastes, and the ...
... kidneys and the liver are closely associated with the circulatory system as they perform the excretory function of removing waste from the blood. Recognize that kidneys remove nitrogenous wastes, and the ...
Supporting Online Material for
... Fig. S5. The high molecular weight forms of Cry2 are polyubiquitylated species of the protein. In vitro ubiquitin ligation assays of recombinant Cry2 protein were conducted in the presence of recombinant SCFFbxl3. Samples were incubated at 30°C for the indicated time, and reactions were then stopped ...
... Fig. S5. The high molecular weight forms of Cry2 are polyubiquitylated species of the protein. In vitro ubiquitin ligation assays of recombinant Cry2 protein were conducted in the presence of recombinant SCFFbxl3. Samples were incubated at 30°C for the indicated time, and reactions were then stopped ...
Blank Jeopardy - Lisle CUSD 202
... The process that moves substances from areas of low concentration to areas of high concentration. ...
... The process that moves substances from areas of low concentration to areas of high concentration. ...
The co-ordination of cell division, differentiation and morphogenesis
... (G) Section (7 lm) through the apex of a wild-type tobacco plant which has been hybridized with an anti-sense probe for NTH15, encoding a KNOX1like protein. Signal (purple) is seen throughout the meristem (including the I2 position) but is excluded from the I1 position. (H) Longitudinal section thro ...
... (G) Section (7 lm) through the apex of a wild-type tobacco plant which has been hybridized with an anti-sense probe for NTH15, encoding a KNOX1like protein. Signal (purple) is seen throughout the meristem (including the I2 position) but is excluded from the I1 position. (H) Longitudinal section thro ...
FULV-EX 13-0-5 - Growth Products
... penetrating roots and then quickly transports to the shoots of plants. Fulvic acid relieves oxygen deficiency and increases the vital activity of cells. Fulvic acids improves plant metabolism of carbohydrates, resulting in an accumulation of soluble sugars. These soluble sugars increase the pressure ...
... penetrating roots and then quickly transports to the shoots of plants. Fulvic acid relieves oxygen deficiency and increases the vital activity of cells. Fulvic acids improves plant metabolism of carbohydrates, resulting in an accumulation of soluble sugars. These soluble sugars increase the pressure ...
HIF-2α phosphorylation by CK1δ promotes erythropoietin secretion
... of erythropoietin (EPO) in two hepatic cancer cell lines, Huh7 and HepG2, without affecting levels of HIF-2α protein expression. Furthermore, when CK1δ-dependent phosphorylation of HIF-2α was inhibited, we observed significant cytoplasmic mislocalization of HIF-2α, which was reversed upon addition o ...
... of erythropoietin (EPO) in two hepatic cancer cell lines, Huh7 and HepG2, without affecting levels of HIF-2α protein expression. Furthermore, when CK1δ-dependent phosphorylation of HIF-2α was inhibited, we observed significant cytoplasmic mislocalization of HIF-2α, which was reversed upon addition o ...
A1984SX34500001
... proposed, for example, that histamine's ability to inhibit its own release might constitute the basis of an inhibitory feedback loop that would serve to inhibit the extent or intensity of allergic responses. Subsequent experiments (summarized in reference 2) provided suggestive evidence in favor of ...
... proposed, for example, that histamine's ability to inhibit its own release might constitute the basis of an inhibitory feedback loop that would serve to inhibit the extent or intensity of allergic responses. Subsequent experiments (summarized in reference 2) provided suggestive evidence in favor of ...
Selecting B cells and plasma cells to memory
... cells if they find appropriate survival conditions outside of restimulating secondary lymphoid organs. Thus, similar to plasma cells, there might be shortand long-lived memory B cells likely determined by their affinity for the respective antigen and their survival potency, which is determined by th ...
... cells if they find appropriate survival conditions outside of restimulating secondary lymphoid organs. Thus, similar to plasma cells, there might be shortand long-lived memory B cells likely determined by their affinity for the respective antigen and their survival potency, which is determined by th ...
Arabidopsis WPP-Domain Proteins Are
... 46.7% amino acid identity, respectively, compared with 28.9% identity with WPP3, 27.5% with RanGAP1, and 28.3% with RanGAP2). WPP1 and WPP2 are more closely related to each other (56.1% amino acid identity) than to WPP3 (34.8 and 43.9% amino acid identity, respectively), and all three family members ...
... 46.7% amino acid identity, respectively, compared with 28.9% identity with WPP3, 27.5% with RanGAP1, and 28.3% with RanGAP2). WPP1 and WPP2 are more closely related to each other (56.1% amino acid identity) than to WPP3 (34.8 and 43.9% amino acid identity, respectively), and all three family members ...
Supporting information Highly sensitive and specific protein
... nM in PLA buffer (1 mM D-biotin (B1595, ThermoFisher Scientific), 0.1% BSA, 0.05% Tween 20, 100 nM goat IgG (I5256, Sigma-Aldrich), 0.1 μg/μl salmon sperm DNA (15632-011, ThermoFisher Scientific), 5 mM EDTA in PBS) and incubated at room temperature for 5 min. The antibody-DNA conjugates were then mi ...
... nM in PLA buffer (1 mM D-biotin (B1595, ThermoFisher Scientific), 0.1% BSA, 0.05% Tween 20, 100 nM goat IgG (I5256, Sigma-Aldrich), 0.1 μg/μl salmon sperm DNA (15632-011, ThermoFisher Scientific), 5 mM EDTA in PBS) and incubated at room temperature for 5 min. The antibody-DNA conjugates were then mi ...
Complex Viruses - HCC Learning Web
... permanently alter its genetic material resulting in cancer – transformation of the cell • Transformed cells have an increased rate of growth, alterations in chromosomes, and the capacity to divide for indefinite time periods resulting in tumors • Mammalian viruses capable of initiating tumors are ca ...
... permanently alter its genetic material resulting in cancer – transformation of the cell • Transformed cells have an increased rate of growth, alterations in chromosomes, and the capacity to divide for indefinite time periods resulting in tumors • Mammalian viruses capable of initiating tumors are ca ...
Ex vivo analysis of splicing assays
... an example of ex vivo splicing analysis. 2. Transfecting adherent cell lines: There are three main kinds of methods to transiently or stably transfect adherent cells. 1. Calcium phosphate based transfection has been used for nearly 40 years to transfect plasmid DNA into cultured cells. DNA is introd ...
... an example of ex vivo splicing analysis. 2. Transfecting adherent cell lines: There are three main kinds of methods to transiently or stably transfect adherent cells. 1. Calcium phosphate based transfection has been used for nearly 40 years to transfect plasmid DNA into cultured cells. DNA is introd ...
Selecting B cells and plasma cells to memory
... cells if they find appropriate survival conditions outside of restimulating secondary lymphoid organs. Thus, similar to plasma cells, there might be shortand long-lived memory B cells likely determined by their affinity for the respective antigen and their survival potency, which is determined by th ...
... cells if they find appropriate survival conditions outside of restimulating secondary lymphoid organs. Thus, similar to plasma cells, there might be shortand long-lived memory B cells likely determined by their affinity for the respective antigen and their survival potency, which is determined by th ...
RNA interference: the new somatic cell genetics?
... residue is required for efficient initiation (Goomer and Kunkel, 1992). Second, one report suggests that adding the leader sequence of 27 nt from the U6 snRNA improves expression (Paul et al., 2002). Third, there is in vitro data to suggest that the mouse U6 promoter may be more active in human cell ...
... residue is required for efficient initiation (Goomer and Kunkel, 1992). Second, one report suggests that adding the leader sequence of 27 nt from the U6 snRNA improves expression (Paul et al., 2002). Third, there is in vitro data to suggest that the mouse U6 promoter may be more active in human cell ...
Classification: BIOLOGICAL SCIENCES: Medical Sciences
... hemoglobin is affected by several factors, such as temperature, pH, Hb variants, and the ratio of 2-3 DPG to hemoglobin. This data, and the rest of the data in this paper unless otherwise stated, was collected at 22˚C and a pH of 5.5, due to the acidic properties of BSA. For the curve plotted in Fig ...
... hemoglobin is affected by several factors, such as temperature, pH, Hb variants, and the ratio of 2-3 DPG to hemoglobin. This data, and the rest of the data in this paper unless otherwise stated, was collected at 22˚C and a pH of 5.5, due to the acidic properties of BSA. For the curve plotted in Fig ...
THP-1 - Cancer Research
... membrane-related processes, studying these changes in mem brane properties during the differentiation and functional matura tion of macrophages is important. Tissue TGase, an intracellular enzyme that catalyzes the covalent cross-linking of proteins via f-(7-glutaminyl)lysine isopeptide bonds (5), a ...
... membrane-related processes, studying these changes in mem brane properties during the differentiation and functional matura tion of macrophages is important. Tissue TGase, an intracellular enzyme that catalyzes the covalent cross-linking of proteins via f-(7-glutaminyl)lysine isopeptide bonds (5), a ...
Cell culture
Cell culture is the process by which cells are grown under controlled conditions, generally outside of their natural environment. In practice, the term ""cell culture"" now refers to the culturing of cells derived from multicellular eukaryotes, especially animal cells, in contrast with other types of culture that also grow cells, such as plant tissue culture, fungal culture, and microbiological culture (of microbes). The historical development and methods of cell culture are closely interrelated to those of tissue culture and organ culture. Viral culture is also related, with cells as hosts for the viruses. The laboratory technique of maintaining live cell lines (a population of cells descended from a single cell and containing the same genetic makeup) separated from their original tissue source became more robust in the middle 20th century.