Steps
... scientist and physician Hans Christian Joachim Gram in 1884. Steps : Heat fixed smear of specimen is stained with 2% crystal violet solution for one minute. ...
... scientist and physician Hans Christian Joachim Gram in 1884. Steps : Heat fixed smear of specimen is stained with 2% crystal violet solution for one minute. ...
Cytologic Sampling Techniques
... and to have them immersed in for at least 20 minutes. Routine Stains For cytological diagnosis, fixed material is usually stained by Papanicolaou Stain (which is preferred) or by Hematoxylin and Eosin (H&E) method. Both employ one of the Hematoxylins for staining of the nuclei. This dye colors the n ...
... and to have them immersed in for at least 20 minutes. Routine Stains For cytological diagnosis, fixed material is usually stained by Papanicolaou Stain (which is preferred) or by Hematoxylin and Eosin (H&E) method. Both employ one of the Hematoxylins for staining of the nuclei. This dye colors the n ...
advanced non-invasive techniques in diagnosis of oral
... examination of cells obtained by their physical ...
... examination of cells obtained by their physical ...
A Definitive, Rapid Alternative to the Gram Stain Assay
... The Gram stain assay differentiates bacteria into two groups. Gram positive organisms have high levels of peptidoglycan in their cell walls, which retain the primary crystal violet stain and appear purple. The Gram negative cell wall contains small levels of peptidoglycan but also contains an outer ...
... The Gram stain assay differentiates bacteria into two groups. Gram positive organisms have high levels of peptidoglycan in their cell walls, which retain the primary crystal violet stain and appear purple. The Gram negative cell wall contains small levels of peptidoglycan but also contains an outer ...
Gram Staining Method
... Attach the clothes pin to one end of your slide Place 2-3 drops of Crystal Violet Primary Stain inside your circle (on your bacteria) Leave the stain on the bacteria sample for one minute Rinse stain off with water (gently) ...
... Attach the clothes pin to one end of your slide Place 2-3 drops of Crystal Violet Primary Stain inside your circle (on your bacteria) Leave the stain on the bacteria sample for one minute Rinse stain off with water (gently) ...
Lab #2
... 1. Place a slide with a bacterial smear on a staining rack. 2. STAIN the slide with crystal violet for 1-2 min. 3. Pour off the stain and rinse with water thoroughly. 4. Flood slide with Gram's iodine for 1-2 min. 5. Pour off the iodine and rinse with water thoroughly. 6. Decolorize by washing the s ...
... 1. Place a slide with a bacterial smear on a staining rack. 2. STAIN the slide with crystal violet for 1-2 min. 3. Pour off the stain and rinse with water thoroughly. 4. Flood slide with Gram's iodine for 1-2 min. 5. Pour off the iodine and rinse with water thoroughly. 6. Decolorize by washing the s ...
Chapter 3
... acid-alcohol, and are usually counterstained (with a different color basic stain) to see them. = blue cells ...
... acid-alcohol, and are usually counterstained (with a different color basic stain) to see them. = blue cells ...
Fluorescent Cell Imaging Activities for Your Classroom - Bio-Rad
... (135-1303EDU) or PureBlu Hoechst 33342 Nuclear Staining Dye (135-1304EDU) and watch the nucleus of the fertilized egg divide numerous times within the cytoplasm, generating a large number of nuclei. Approximately four hours after fertilization, after 13 mitotic divisions, an estimated 6,000 nuclei w ...
... (135-1303EDU) or PureBlu Hoechst 33342 Nuclear Staining Dye (135-1304EDU) and watch the nucleus of the fertilized egg divide numerous times within the cytoplasm, generating a large number of nuclei. Approximately four hours after fertilization, after 13 mitotic divisions, an estimated 6,000 nuclei w ...
Chap. 23 : Bacteria - Fort Thomas Independent Schools
... 4. Fix nitrogen (change N to a form we can use) 5. Used as medicines (antibiotics) ...
... 4. Fix nitrogen (change N to a form we can use) 5. Used as medicines (antibiotics) ...
Cell membrane
... during the short decolorization step and the bacteria remain purple. In contrast, gram-negative bacteria peptidoglycan is very thin, not as highly cross-linked, and has larger pores. Alcohol treatment also may extract enough lipid from the gram-neaative wall to increase its porosity further. For the ...
... during the short decolorization step and the bacteria remain purple. In contrast, gram-negative bacteria peptidoglycan is very thin, not as highly cross-linked, and has larger pores. Alcohol treatment also may extract enough lipid from the gram-neaative wall to increase its porosity further. For the ...
Gram`s staining - Micro-Rao
... The smear is treated with few drop of Gram's Iodine and allowed to act for a minute. This results in formation of a dye-iodine complex in the cytoplasm. Gram's iodine serves as a mordant. The slide is again washed in water and then decolorized in absolute ethyl alcohol or acetone. A mixture of eceto ...
... The smear is treated with few drop of Gram's Iodine and allowed to act for a minute. This results in formation of a dye-iodine complex in the cytoplasm. Gram's iodine serves as a mordant. The slide is again washed in water and then decolorized in absolute ethyl alcohol or acetone. A mixture of eceto ...
ZIEHL-NEELSEN for acid-fast bacteria 04 – 111802/L
... Product for the preparation of cyto-histological samples for optical microscopy. ...
... Product for the preparation of cyto-histological samples for optical microscopy. ...
The Plant Cell: Peeping into Potatoes, Peppers, and Pears
... Remove a leaf from the young tip of an Elodea sprig and place on a slide with a drop of water. The cells on the upper surface of the leaf will be bigger and easier to examine. Add a coverslip and place under the scope. 2. Focus up and down through the layers of the cells. Estimate how many layers th ...
... Remove a leaf from the young tip of an Elodea sprig and place on a slide with a drop of water. The cells on the upper surface of the leaf will be bigger and easier to examine. Add a coverslip and place under the scope. 2. Focus up and down through the layers of the cells. Estimate how many layers th ...
Paraffin Histology
... Formalin is a commonly used fixative. Other fixatives may contain one or more of the following - mercuric chloride, picric acid, acetic acid, ethanol, chloroform, and potassium dichromate. These may be used singly, together, or sequentially. Fixation may also be carried out using microwaves. ALL of ...
... Formalin is a commonly used fixative. Other fixatives may contain one or more of the following - mercuric chloride, picric acid, acetic acid, ethanol, chloroform, and potassium dichromate. These may be used singly, together, or sequentially. Fixation may also be carried out using microwaves. ALL of ...
Introduction to Pathology Course
... of iron in blood cells. • Small amounts of ferric iron are found normally in the spleen and bone marrow. Excessive amounts are present in hemochromatosis and ...
... of iron in blood cells. • Small amounts of ferric iron are found normally in the spleen and bone marrow. Excessive amounts are present in hemochromatosis and ...
Path review
... comes into lungs, bacteria grows, and it starts to have the consistency of liver 6-arrows- alveoli filled w/ neutrophils and edema Bronchopneumonia -only involves segmental bronchi 1- abscesses- lighter area on left of lung is where the pneumonia infection is taking place 3- Abscesses forming 2-lost ...
... comes into lungs, bacteria grows, and it starts to have the consistency of liver 6-arrows- alveoli filled w/ neutrophils and edema Bronchopneumonia -only involves segmental bronchi 1- abscesses- lighter area on left of lung is where the pneumonia infection is taking place 3- Abscesses forming 2-lost ...
Correlation of Conjunctival and Corneal Staining With Elevated
... correlates significantly with ocular surface staining • This supports evidence that there is an underlying inflammatory process ongoing in the ocular surface of these eyes • Detection of ocular surface inflammation can be used clinically to guide anti-inflammatory therapy in dry eye patients ...
... correlates significantly with ocular surface staining • This supports evidence that there is an underlying inflammatory process ongoing in the ocular surface of these eyes • Detection of ocular surface inflammation can be used clinically to guide anti-inflammatory therapy in dry eye patients ...
Vascular tissue microscopy - teacher notes
... Fresh celery from a supermarket is good and many different plant stems can be found around the fruit and veg., section as well as from the cut flowers and pot plants section. We advise against using plant material with very tough stems as sectioning may become more hazardous. For best results keep p ...
... Fresh celery from a supermarket is good and many different plant stems can be found around the fruit and veg., section as well as from the cut flowers and pot plants section. We advise against using plant material with very tough stems as sectioning may become more hazardous. For best results keep p ...
Idioblastic mucilage - Modern Phytomorphology
... constitution (mucilage), as histochemical tests showed. Idioblastic cells, therefore, correspond to typical mucilage cells. Mucilage seems to play a crucial role in the adaptation of the plant to unfavourable environmental conditions. Key words: Teucrium polium, mucilage cells, anatomy, histochemist ...
... constitution (mucilage), as histochemical tests showed. Idioblastic cells, therefore, correspond to typical mucilage cells. Mucilage seems to play a crucial role in the adaptation of the plant to unfavourable environmental conditions. Key words: Teucrium polium, mucilage cells, anatomy, histochemist ...
Histology - Introduction
... suitable fixative must be added. These prevent the protein enzymes from functioning. The most famous fixative used is Formalin (an aqueous solution of formaldehyde) which is used to preserve ...
... suitable fixative must be added. These prevent the protein enzymes from functioning. The most famous fixative used is Formalin (an aqueous solution of formaldehyde) which is used to preserve ...
Gram Staining and Cell Wall Structure
... Most bacteria secrete a covering for themselves which we call a cell wall, However, bacterial cell walls are a totally different thing than the cell walls we talk about plants having. Bacterial cell walls do NOT contain cellulose like plant cell walls do. Bacterial cell walls are made mostly of a ch ...
... Most bacteria secrete a covering for themselves which we call a cell wall, However, bacterial cell walls are a totally different thing than the cell walls we talk about plants having. Bacterial cell walls do NOT contain cellulose like plant cell walls do. Bacterial cell walls are made mostly of a ch ...
Staining
Staining is an auxiliary technique used in microscopy to enhance contrast in the microscopic image. Stains and dyes are frequently used in biology and medicine to highlight structures in biological tissues for viewing, often with the aid of different microscopes. Stains may be used to define and examine bulk tissues (highlighting, for example, muscle fibers or connective tissue), cell populations (classifying different blood cells, for instance), or organelles within individual cells.In biochemistry it involves adding a class-specific (DNA, proteins, lipids, carbohydrates) dye to a substrate to qualify or quantify the presence of a specific compound. Staining and fluorescent tagging can serve similar purposes. Biological staining is also used to mark cells in flow cytometry, and to flag proteins or nucleic acids in gel electrophoresis.Simple staining is staining with only one stain/dye. There are various kinds of multiple staining, many of which are examples of counterstaining, differential staining, or both, including double staining and triple staining. Staining is not limited to biological materials, it can also be used to study the morphology of other materials for example the lamellar structures of semi-crystalline polymers or the domain structures of block copolymers.