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Bacteria - Ms. Pass's Biology Web Page
Bacteria - Ms. Pass's Biology Web Page

... ability to retain crystal violet dye during solvent treatment. Safranin is added as a mordant to form the crystal violet/safranin complex in order to render the dye impossible to remove. Ethyl-alcohol solvent acts as a decolorizer and dissolves the lipid layer from gram-negative cells. This enhances ...
Bacteria
Bacteria

... ability to retain crystal violet dye during solvent treatment. Safranin is added as a mordant to form the crystal violet/safranin complex in order to render the dye impossible to remove. Ethyl-alcohol solvent acts as a decolorizer and dissolves the lipid layer from gram-negative cells. This enhances ...
Unit II: Bacterial Morphology and Cellular Structures
Unit II: Bacterial Morphology and Cellular Structures

... combine with the same charge cellular components. In fact, these acidic stains are repelled from the cells surface. Because the acidic stain will bind to the glass slide but not the cells, the result is a dark stained background and clear, colorless, cells. This staining technique is very useful bec ...
1-Introduction to histology
1-Introduction to histology

... ---Development of histology deponds on the development of technique. ---Histology studies the microstructures. So, we should have the aid of microscope to study. Several types of microscopes are available. According to the light source used, microscopes can be basally classified as:  light microsco ...
Document
Document

... 5 What is the purpose of Gram staining? In Gram staining, the specimen is treated with a primary stain called crystal violet, followed by iodine as a fixative, or mordant. After iodine treatment, the specimen is flushed with alcohol to dehydrate peptidoglycans and trap the stain. Next, the specimen ...
GelDoc-It Imaging System Using GelRed and GelGreen
GelDoc-It Imaging System Using GelRed and GelGreen

... Traditionally, imaging of EtBr gels using tube based UV transilluminators and film has been the means to detect and document nucleic acid bands in gels. However, newer technologies, such as safer, brighter, and simple to use nucleic acid binding dyes and imaging systems that incorporate GelCam 310 c ...
Chapter 1 Introduction
Chapter 1 Introduction

... ---Development of histology deponds on the development of technique. ---Histology studies the microstructures. So, we should have the aid of microscope to study. Several types of microscopes are available. According to the light source used, microscopes can be basally classified as: light microscope ...
Chapter 1 Introduction
Chapter 1 Introduction

... ---Development of histology deponds on the development of technique. ---Histology studies the microstructures. So, we should have the aid of microscope to study. Several types of microscopes are available. According to the light source used, microscopes can be basally classified as: light microscope ...
EPITHELIUM
EPITHELIUM

...  Insert into basal bodies (1 cilium per 1 body)  Motile processes of microtubules move synchronously  9 +2 microtubule arrangement  Ex.: trachea and oviduct  insert into terminal web (stains eosinophilic – pink)  actin skeleton above intermediate filaments  increase surface area for absorptio ...
Disappearing Blue!
Disappearing Blue!

... MethyleneBlue Plus™ back to its blue form. When the dissolved oxygen has been consumed, the FlashBlue™ or Methylene Blue Plus™ is slowly reduced back to its colorless form by the remaining glucose, and the cycle can be repeated many times by further shaking. A white background helps to make the colo ...
Specification sheet
Specification sheet

... PR178-6ml RTU PR178-3ml RTU CR178-0.1ml Conc CR178-0.5ml Conc HAR178-6ml RTU HAR178-3ml RTU ...
integument - utcom2010
integument - utcom2010

...  Pink cytoplasm, less densely stained  Nucleus off to one side 5 - Reticulocyte  Special stain shows blue particles (rough ER) within cell  No nucleus  Normally only 1% of blood smear 6 - RBC – 7 um Promyelocyte  Large, central nucleus, many nucleoli  Prominent granules in cytoplasm (azurophi ...
What are antibiotics?
What are antibiotics?

... Gram negative: A group of bacteria that do not retain the crystal violet dye after the differential staining procedure known as Gram staining. They appear pink due to the counterstain, safranin. Gram positive appears purple. The difference between Gram negative and Gram positive bacteria is the cell ...
Specification sheet
Specification sheet

... IVD This antibody is intended for use to qualitatively Status identify Calcitonin by light microscopy in formalin fixed, paraffin embedded tissue sections using immunohistochemical detection methodology. Interpretation of any positive or negative staining must be complemented with the evaluation of ...
Electron Microscope
Electron Microscope

... The organella - nucleus (DNA, RNA), and regions of the cytoplasm rich in ◦ ribosomes or another acidic components are stain dark blue; The components stain with hematoxylin are referred to as basophilic. ◦ ...
Lesson 3 WT neisseria infections
Lesson 3 WT neisseria infections

... cells for bacteria and NAD present in ery - factor X a V • Blood agar - Stafylococcus aureus spreads NAD (V factor) – in his environment Haemophilus can grow • Requiremnt of X or V or both factors serve for idnetificatio of heamophilus sp - H.i. – need both XV, H. parainfluenzae only V ...
Microscopy and Cell Structure
Microscopy and Cell Structure

...  Basic dyes carry positive charge and bond to cell structures ...
Wet electron microscopy with quantum dots Winston Timp1, Nicki
Wet electron microscopy with quantum dots Winston Timp1, Nicki

... structures in the cell from one another and from the background. Resolution refers to the minimum separation between objects required to identify them as individual structures. In order to effectively use microscopy to explore the biological landscape, the sample must have sufficient contrast and re ...
Document
Document

... and used to build a composite image replicas of fractured surfaces; (c) or representation. scanning electron microscopy (SEM). Most materials and structures cannot be stained and viewed at the same time; stains are used selectively to give a partial picture, e.g. a stain for mucus counterstained to ...
Bacteria
Bacteria

... – Developed by Hans Christian Gram in 1884 – Helps to identify different types of bacteria (a differential stain) – Stain uses differences in cell wall composition to differentiate between bacteria – Can help determine which type of antibiotics will be most effective against a particular bacteria ...
m5zn_aa487bab657cf4d
m5zn_aa487bab657cf4d

...  preserves overall morphology but not internal structures  chemical fixation – used with larger, more delicate organisms  protects fine cellular substructure and morphology ...
Architecture of dorsal tissue aggregates from frog embryos.
Architecture of dorsal tissue aggregates from frog embryos.

... form of clinically needed tissues together with their proper The dissociated cells are placed in a specially designed function. In tissue engineering today, 3D-printing is the newest microcentrifuge tube with 30 µL of agarose in a micropipette way to generate multicellular tissues for clinical use.[ ...
Lab 9-Proeukaryote
Lab 9-Proeukaryote

... Macroscopic characteristics such as these are used to help identify what kind of bacteria constitute these colonies. What are the most common colony shapes, colony margins and colony surface characteristics found in the species you observed on the demonstration bench? Microscopic observation of bact ...
Exam 1 - web.biosci.utexas.edu
Exam 1 - web.biosci.utexas.edu

... (Colony Forming Units/ ml). Don't forget to divide by the amount plated when you are calculating the original titer of your stock. 3. Pour plate and Spread plate techniques differences and similarities. 4. Micropipettors: P1000, P200, P20 how to read the settings. 5. 30-300 is the statistically sign ...
lec3
lec3

... Cells + fibers + ground substance = connective tissue Cells = fibroblasts Fibers = elastin & collagen Reticular fibers ...
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Staining



Staining is an auxiliary technique used in microscopy to enhance contrast in the microscopic image. Stains and dyes are frequently used in biology and medicine to highlight structures in biological tissues for viewing, often with the aid of different microscopes. Stains may be used to define and examine bulk tissues (highlighting, for example, muscle fibers or connective tissue), cell populations (classifying different blood cells, for instance), or organelles within individual cells.In biochemistry it involves adding a class-specific (DNA, proteins, lipids, carbohydrates) dye to a substrate to qualify or quantify the presence of a specific compound. Staining and fluorescent tagging can serve similar purposes. Biological staining is also used to mark cells in flow cytometry, and to flag proteins or nucleic acids in gel electrophoresis.Simple staining is staining with only one stain/dye. There are various kinds of multiple staining, many of which are examples of counterstaining, differential staining, or both, including double staining and triple staining. Staining is not limited to biological materials, it can also be used to study the morphology of other materials for example the lamellar structures of semi-crystalline polymers or the domain structures of block copolymers.
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