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High-throughput screens for fluorescent dye discovery
... for most proteins. If enough is known about a biological pathway, an investigator can readily create antibodies to proteins known to be expressed differently in the cellular compartment, state or cell type of interest. For instance, an antibody to a well-known marker of mitosis, phosphorylated histo ...
... for most proteins. If enough is known about a biological pathway, an investigator can readily create antibodies to proteins known to be expressed differently in the cellular compartment, state or cell type of interest. For instance, an antibody to a well-known marker of mitosis, phosphorylated histo ...
Bacterial Anatomy
... Intracytoplasmic granules - inclusion bodies or metachromatic granules for storage of energy polymers such as glycogen Ribosomes - ribonucleoprotein complexes upon which protein synthesis occurs ...
... Intracytoplasmic granules - inclusion bodies or metachromatic granules for storage of energy polymers such as glycogen Ribosomes - ribonucleoprotein complexes upon which protein synthesis occurs ...
Compartmentalization of Cyclic GMP
... FMLP stimulated transient focal changes in G-kinase localitation that coincided with transient changes in cell shape. Within 30 seconds intense focal staining of G-kinase was observed along the cell margin and at the trailing end of polarized cells (Fig 2B). After I minute, when most of the neutroph ...
... FMLP stimulated transient focal changes in G-kinase localitation that coincided with transient changes in cell shape. Within 30 seconds intense focal staining of G-kinase was observed along the cell margin and at the trailing end of polarized cells (Fig 2B). After I minute, when most of the neutroph ...
Mycobacterium tuberculosis
... Slow-growing with a generation time of 12 to 18 hours (c.f. 20-30 minutes for Escherichia coli). So grow sloly in branching chains resembling fungal hyphae. Hydrophobic with a high lipid content in the cell wall. Because the cells are hydrophobic and tend to clump together, they are impermeable to t ...
... Slow-growing with a generation time of 12 to 18 hours (c.f. 20-30 minutes for Escherichia coli). So grow sloly in branching chains resembling fungal hyphae. Hydrophobic with a high lipid content in the cell wall. Because the cells are hydrophobic and tend to clump together, they are impermeable to t ...
Classification of Gram
... – Sputum in pneumonic anthrax – Stool in intestinal anthrax (also in food poisoning by B. cereus) ...
... – Sputum in pneumonic anthrax – Stool in intestinal anthrax (also in food poisoning by B. cereus) ...
Counting and imaging bacteria using
... AFM images of bacteria in air often show some sort of “material” adjacent to cells ...
... AFM images of bacteria in air often show some sort of “material” adjacent to cells ...
18.4 – Bacteria and Archaea Prokaryotes are
... that can replicate separately from the main chromosome o Most prokaryotes can move on their own by gliding or using flagella Flagellum – long, whip-like structure outside of a cell that is used for movement o May also have pili – thinner, shorter structures that allow prokaryotes to stick to surfa ...
... that can replicate separately from the main chromosome o Most prokaryotes can move on their own by gliding or using flagella Flagellum – long, whip-like structure outside of a cell that is used for movement o May also have pili – thinner, shorter structures that allow prokaryotes to stick to surfa ...
BD Pharmingen™ Transcription Factor Buffer Set
... Likely no, this is the classic problem of understanding fixation and how it interacts with optics of the cytometer. Cells will appear “smaller” by FSC vs SSC, much like if they have been treated with BD FACS™ lysing solution. Voltages on the detectors are to be increased to gate properly. When compa ...
... Likely no, this is the classic problem of understanding fixation and how it interacts with optics of the cytometer. Cells will appear “smaller” by FSC vs SSC, much like if they have been treated with BD FACS™ lysing solution. Voltages on the detectors are to be increased to gate properly. When compa ...
Let`s move cell health forward together
... (microorganisms or proteins) labeled with pHrodo™ dye are added to cells. Some remain in solution or become nonspecifically attached to cells—they do not fluoresce because of the neutral pH of the extracellular environment. Some are taken up by cells by phagocytosis or endocytosis and become encapsu ...
... (microorganisms or proteins) labeled with pHrodo™ dye are added to cells. Some remain in solution or become nonspecifically attached to cells—they do not fluoresce because of the neutral pH of the extracellular environment. Some are taken up by cells by phagocytosis or endocytosis and become encapsu ...
mcb101_exam-1_F`07
... 42) In the gelatinase test, the plate is flooded with ammonium sulfate after incubation. 43) Iodine forms a blue-black complex when it binds to lipids found in egg yolk. 44) In theMPN test, a positive response for coliforms can be produced by the presence of as few as one viable E. coli cell in the ...
... 42) In the gelatinase test, the plate is flooded with ammonium sulfate after incubation. 43) Iodine forms a blue-black complex when it binds to lipids found in egg yolk. 44) In theMPN test, a positive response for coliforms can be produced by the presence of as few as one viable E. coli cell in the ...
Chapter 5 - Anatomy and Physiology Period 9
... 5.4 Types of Membranes Epithelial membranes – thin, sheetlike structures composed of epithelium and underlying connective tissue covering body surfaces and lining body cavities Serous – secrete serous fluid Mucus – goblet cells ...
... 5.4 Types of Membranes Epithelial membranes – thin, sheetlike structures composed of epithelium and underlying connective tissue covering body surfaces and lining body cavities Serous – secrete serous fluid Mucus – goblet cells ...
Histology
... • Functions: protection, secretion, absorption, excretion. Cancer originating in epithelium? Carcinoma *90% of human cancers *Most begin on surfaces in contact with external environment. ...
... • Functions: protection, secretion, absorption, excretion. Cancer originating in epithelium? Carcinoma *90% of human cancers *Most begin on surfaces in contact with external environment. ...
Microbial Ecology
... ester (FLUOS) (Boehringer Mannheim, Penzberg, Germany), purified as described by Amann et al. [2] and stored at −20°C. For immunological staining of the bacterial cells, strain-specific mAbs for A. brasilense strains Sp7 and Wa3 and a species-specific pAs for Herbaspirillum seropedicae were used. mA ...
... ester (FLUOS) (Boehringer Mannheim, Penzberg, Germany), purified as described by Amann et al. [2] and stored at −20°C. For immunological staining of the bacterial cells, strain-specific mAbs for A. brasilense strains Sp7 and Wa3 and a species-specific pAs for Herbaspirillum seropedicae were used. mA ...
Approach to blue stain fungi on ISPM 15-certified wood
... Actioning of ISPM 15-certified wood packaging that presented with active blue stain fungi was ceased by the Department of Agriculture, Fisheries and Forestry in June 2012. This was because only a small proportion of wood packaging was being inspected, blue stain fungi may be present in wood packagin ...
... Actioning of ISPM 15-certified wood packaging that presented with active blue stain fungi was ceased by the Department of Agriculture, Fisheries and Forestry in June 2012. This was because only a small proportion of wood packaging was being inspected, blue stain fungi may be present in wood packagin ...
Assessment of Aging in Saccharomyces Cerevisiae Yeast Mutants
... increased in conjunction with reduced SOD2 expression . The number of dead cells is one of the parameters that make it possible to determine culture viability, yet it does not provide information on all of the changes that take place in a yeast cell culture. For this reason other symptoms of aging, ...
... increased in conjunction with reduced SOD2 expression . The number of dead cells is one of the parameters that make it possible to determine culture viability, yet it does not provide information on all of the changes that take place in a yeast cell culture. For this reason other symptoms of aging, ...
Cartilage - UTCOM2013
... many more nuclei per specific area in tendon Nervous: Nerve fibers: black, fibers entering muscle---supplying electrical signals for the command structure; voluntary command starts in cerebral cortex, travels through ventral horn, Nissl bodies visible in slides stained for ribosomes (the dendrites w ...
... many more nuclei per specific area in tendon Nervous: Nerve fibers: black, fibers entering muscle---supplying electrical signals for the command structure; voluntary command starts in cerebral cortex, travels through ventral horn, Nissl bodies visible in slides stained for ribosomes (the dendrites w ...
Content of Lectures Given in Pharmaceutical Microbiology II (PHT 313)
... characterize the spores of Clostridium. Nagler's reaction and Sormy clot formation to identify Clostridium. Gram stains to characterize Gram-negative bacilli. Oxidase test, nitrate reductase test and oxidative fermentative test to identify Enterobacteriaceae. Use of common selective and differential ...
... characterize the spores of Clostridium. Nagler's reaction and Sormy clot formation to identify Clostridium. Gram stains to characterize Gram-negative bacilli. Oxidase test, nitrate reductase test and oxidative fermentative test to identify Enterobacteriaceae. Use of common selective and differential ...
Microscopy and Immunoassays
... • Most common of all light scopes • Light is transmitted through specimen • Specimen appears darker than surrounding field • Typical use: Gram Stains ...
... • Most common of all light scopes • Light is transmitted through specimen • Specimen appears darker than surrounding field • Typical use: Gram Stains ...
Animal Tissues
... determines classification of connective tissues two components – ground substance – fibers ...
... determines classification of connective tissues two components – ground substance – fibers ...
Automated Signal Counting for SISH, Dual CISH
... Affymetrix ViewRNA Example In this example the algorithm is tuned for the Affymetrix ViewRNA stain colors. It detects nuclei (blue), cytoplasmic regions (yellow), and RNA signals (red). ...
... Affymetrix ViewRNA Example In this example the algorithm is tuned for the Affymetrix ViewRNA stain colors. It detects nuclei (blue), cytoplasmic regions (yellow), and RNA signals (red). ...
Lab 6 – Bacterial motility
... 3. Flagella stain • Flagella are too thin to be seen by the ordinary light microscope. • Flagella should be amplified (enlarged). Use a stain that is specifically deposited on Flagella thus increasing diameter. • Some flagellar stains employ rosaniline dyes and a mordant, applied to a bacterial sus ...
... 3. Flagella stain • Flagella are too thin to be seen by the ordinary light microscope. • Flagella should be amplified (enlarged). Use a stain that is specifically deposited on Flagella thus increasing diameter. • Some flagellar stains employ rosaniline dyes and a mordant, applied to a bacterial sus ...
Intradermal Delivery of Methylene Blue using hMTS for Sentinel
... as a new standard for staging breast cancer. Methylene blue (MB) is routinely used as a dye for mapping the sentinel lymph node associated with a tumor. Among available delivery routes, intradermal delivery is of interest due to the lymphatic capillaries that perfuse the intradermal compartment. How ...
... as a new standard for staging breast cancer. Methylene blue (MB) is routinely used as a dye for mapping the sentinel lymph node associated with a tumor. Among available delivery routes, intradermal delivery is of interest due to the lymphatic capillaries that perfuse the intradermal compartment. How ...
Human Tissues I
... c. Next, the specimen is embedded d. You section the specimine with microtome e. Finally, you can stain the specimen with various dyes f. Hematoxylin and Eosin (HE) is most common g. Hematoxylin is blue and stains acidic materials, Eosin is pink and stains basic materials h. If you stained a cell wi ...
... c. Next, the specimen is embedded d. You section the specimine with microtome e. Finally, you can stain the specimen with various dyes f. Hematoxylin and Eosin (HE) is most common g. Hematoxylin is blue and stains acidic materials, Eosin is pink and stains basic materials h. If you stained a cell wi ...
Staining
![](https://commons.wikimedia.org/wiki/Special:FilePath/Microscope_with_stained_slide.jpg?width=300)
Staining is an auxiliary technique used in microscopy to enhance contrast in the microscopic image. Stains and dyes are frequently used in biology and medicine to highlight structures in biological tissues for viewing, often with the aid of different microscopes. Stains may be used to define and examine bulk tissues (highlighting, for example, muscle fibers or connective tissue), cell populations (classifying different blood cells, for instance), or organelles within individual cells.In biochemistry it involves adding a class-specific (DNA, proteins, lipids, carbohydrates) dye to a substrate to qualify or quantify the presence of a specific compound. Staining and fluorescent tagging can serve similar purposes. Biological staining is also used to mark cells in flow cytometry, and to flag proteins or nucleic acids in gel electrophoresis.Simple staining is staining with only one stain/dye. There are various kinds of multiple staining, many of which are examples of counterstaining, differential staining, or both, including double staining and triple staining. Staining is not limited to biological materials, it can also be used to study the morphology of other materials for example the lamellar structures of semi-crystalline polymers or the domain structures of block copolymers.