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Expression of Recombinant Myelin
Expression of Recombinant Myelin

... of neurites and were transferred to either new neuronal cultures or to poly-Llysine-coated coverslips. To recover the Schwann cells from a culture, the neuronal cell bodies were first cut out, the culture was treated with collagenase (0.1%, 30 rain, 37°C) followed by trypsin (0.1%, 30 rain, 37°C), a ...
Physical interaction between proliferating cell nuclear antigen and
Physical interaction between proliferating cell nuclear antigen and

... template, human Replication Factor C (RFC) enhanced the PfuPCNA-dependent activity of Pol δ (Ishino et al. 2001).The functional interaction of Thermococcus fumicolans PCNA with calf thymus Pol δ has also been reported (Henneke et al. 2000).These findings further support the idea that the structure-f ...
Cytokinesis in Tobacco BY-2 and Root Tip Cells: A New Model of
Cytokinesis in Tobacco BY-2 and Root Tip Cells: A New Model of

... cell wall between daughter cells. This process is accomplished by the formation of the phragmoplast that not only builds the new plate but spatially orients it within the cell relative to the whole plant or organ axis. The phragmoplast of higher plant cells has been described as consisting of three ...
Alveolar Macrophages Isolated Directly From
Alveolar Macrophages Isolated Directly From

... whether in vivo–differentiated alveolar macrophages could support full HCMV lytic infection. Although it was demonstrated that alveolar macrophages matured in vitro were permissive to HCMV infection [13, 15, 16], in vivo–differentiated alveolar macrophages have not, to our knowledge, been tested. Al ...
Lysosomes and Fas-mediated liver cell death
Lysosomes and Fas-mediated liver cell death

... Lysosome-mediated apoptosis would result from a destabilization of the lysosomal membrane causing a release of cathepsins into the cytosol. These proteases would stimulate the mitochondrial pathway of apoptosis by contributing to the permeabilization of the outer mitochondrial membrane, perhaps as a ...
article in press - MPI
article in press - MPI

... caudal halves of prospective and formed somites, such as myoD, snail1 (van Eeden et al., 1996), ephrinB2 (Durbin et al., 2000), and deltaC (Jiang et al., 2000), are instead expressed ubiquitously throughout the paraxial mesoderm of fss, whereas rostral marker genes such as fgf8, ephA4, deltaD, mespa ...
Review Article Expression and Function of the Endocannabinoid
Review Article Expression and Function of the Endocannabinoid

... Endocannabinoids are important retrograde modulators of synaptic transmission throughout the nervous system. Cannabinoid receptors are seven transmembrane G-protein coupled receptors favoring Gi/o protein. They are known to play an important role in various processes, including metabolic regulation, ...
The sea urchin animal pole domain is a Six3
The sea urchin animal pole domain is a Six3

... hybridizations (our unpublished observations). The genes were sorted into three groups: those represented maximally in the maternal RNA population (Fig. 2A), those strongly upregulated during cleavage stages (Fig. 2B,C) and those upregulated between late cleavage and early gastrula stages (Fig. 2D). ...
Brennan - Journal of Experimental Biology
Brennan - Journal of Experimental Biology

... and Schroeder, 1928). A genetic discontinuity exists between geographically proximate populations along parallel salinity clines that transition between brackish and fresh waters of the eastern US despite no apparent barriers to gene flow. Freshwater populations show an increased capacity to acclima ...
Membrane permeability of the human granulocyte to
Membrane permeability of the human granulocyte to

... mononuclear leukocytes, granulocytes and red blood cells. The band corresponding to the granulocytes (i.e., the second band from the top) was carefully removed from each 15 mL centrifuge tube using a 1 mL micropipette and placed into a single 50 mL centrifuge tube. This resulted in a total volume of ...
Intrusive growth of flax phloem fibers is of intercalary type
Intrusive growth of flax phloem fibers is of intercalary type

... Instead, organelles moved into the very tip region, and cortical microtubules showed transversal and helical configurations as known for cells growing in intercalary way. In addition, pulse-chase experiments with Calcofluor White resulted in a spotted fluorescence in the cell wall all over the lengt ...
Proteomics of Plasma Membranes from Poplar Trees Reveals
Proteomics of Plasma Membranes from Poplar Trees Reveals

... Published, MCP Papers in Press, November 28, 2009, DOI 10.1074/mcp.M900289-MCP200 ...
Test Bank - rsffa.org
Test Bank - rsffa.org

... ____ 14. A cell in a multicellular organism must be able to carry out all of the functions necessary for life in order for the organism to survive. _________________________ ____ 15. There is a division of labor among the cells of multicellular organisms. _________________________ Completion Complet ...
Monitoring metabolic responses to chemotherapy initiator mass spectrometry (NIMS) imaging
Monitoring metabolic responses to chemotherapy initiator mass spectrometry (NIMS) imaging

... in lymphoid cells by LC-MS/MS [13]. Still, sample preparation for such methods requires tissue extraction, sacrificing anatomical resolution for analytical sensitivity [14,15], highlighting the need for improved metabolomics methodologies. Advancements in mass spectrometry imaging (MSI) and profilin ...
“Understanding dissemination of Mycobacterium tuberculosis from
“Understanding dissemination of Mycobacterium tuberculosis from

... alveolar barriers in triplicate for each strain. Inocula CFUs were verified by plating serial ...
From segment to somite: Segmentation to
From segment to somite: Segmentation to

... gradient specifies the chemical prepattern. Cells of the PSM that lie anterior to the determination front will follow a specific developmental pathway, which cannot be altered by subsequent perturbation of the clock or wavefront. At the anterior end of the PSM, cells undergo changes in their morpholog ...
Aerobic Fermentation and the Depletion of the Amino Acid Pool in
Aerobic Fermentation and the Depletion of the Amino Acid Pool in

... from the shaker and 2.5 ml samples of the suspension were drawn off for analysis of the levels of the amino acid pools and, in certain instances, of the amino acids which leaked out of the cells. T h e zero-time samples were taken from 10 ml cell suspensions to which 0.5 ml of water had been added. ...
the process of selection of erythromycin
the process of selection of erythromycin

... this pair are presented in Figs. 4-10. The genetical results are summarized in Table 1. The method of numbering the cells is indicated on Fig. 1. 1st day. Cell 14a (Fig. 4). After 24 h in ERY the first mitochondrial alterations appear: beginning with loss of cristae, reduction of mitochondrial diame ...
Enzyme Activities Associated with Carbohydrate
Enzyme Activities Associated with Carbohydrate

... seen, but such blastospores did not form germ tubes. Thus enzyme activity at 4 h is of particular interest in relation to the maximal production of germ tubes at this time. Enzyme activities. These have been determined in both growth forms at 3, 4, 5 , 10, 14 and 18 h after inoculation and in the ce ...
Cell Structure and Function
Cell Structure and Function

... 1. All organisms are made of cells 2. All existing cells are produced by other living cells 3. The cell is the most basic unit of life ...
Enhancer timing of Hox gene expression: deletion of
Enhancer timing of Hox gene expression: deletion of

... by retinoic acid (RA) prematurely (Bel-Vialar et al., 2000). These studies suggest that in the initiation phase, a repressive regulatory mechanism is involved in regulating the correct temporal activation of Hox genes by modulating chromatin structure. Therefore, Hox genes must release sequentially ...
Effector-triggered defence against apoplastic fungal pathogens
Effector-triggered defence against apoplastic fungal pathogens

... PTI is rapidly activated by extracellular host recognition of PAMPs by pattern recognition receptors (PRRs); most PRRs are receptor-like kinases with an extracellular receptor domain, a transmembrane domain, and typically a cytoplasmic kinase domain. Pathogens produce effectors to suppress PTI and c ...
how proteins move lipids and lipids move proteins
how proteins move lipids and lipids move proteins

... and membrane dynamics. Its intricacy is illustrated by the known involvement of close to a hundred (isoforms of) kinases and phosphatases in the production and inactivation of signalling inositol lipids (BOX 1), and by the specific recognition of these lipids by receptor domains in at least another ...
Chapter 4
Chapter 4

... a number of fundamental chemical reactions in cells, including formation of peptide bonds between amino acids during protein synthesis. Deoxyribonucleic acid (DNA) contains all the information required to build the cells and tissues of an organism. The exact replication of this information in any sp ...
control of cell division of the intracellular chlorella symbionts in
control of cell division of the intracellular chlorella symbionts in

... Green hydra exercises strict control over the number and reproduction of its intracellular algal symbionts. This control appears to be exercised at the level of the host digestive cell. Contrary to the suggestion of Pardy & Heacox (1976), the head appears to have no influence on algal reproduction i ...
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