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Varicella-Zoster Virus-Infected Human Sensory Neurons Are
Varicella-Zoster Virus-Infected Human Sensory Neurons Are

... Apoptosis or programmed cell death is an energy-dependent process of cell suicide that is characterized by several morphological features, including cell shrinkage, chromatin condensation, DNA fragmentation, membrane asymmetry, and plasma membrane blebbing (72, 73, 78). Apoptosis plays an important ...
Mode Suppressed TEM Cell Design For High Frequency IC
Mode Suppressed TEM Cell Design For High Frequency IC

... is evaluated by using the full wave simulation tool CST Microwave Studio. Figure 7 shows the S parameter results for the modified TEM cell model with only the parallel traces on the walls. For the cell with only the parallel traces, the TE modes still disturb the TEM field in the cell at resonant fr ...
Session 381 Lens physiology and biomechanics II
Session 381 Lens physiology and biomechanics II

... allows quantitative evaluation of the amount of phospholipids and cholesterol in lipid domains of intact fiber-cell plasma membranes isolated from cortical and nuclear regions of eye lenses. This new method will complement the existing method, which is based on an analysis of conventional EPR spectr ...
Immunological Memory: Contribution of Memory B Cells Expressing
Immunological Memory: Contribution of Memory B Cells Expressing

... washed twice in staining buffer (2% FCS in PBS), immediately acquired by flow cytometry using FACSort (Becton Dickinson), and subsequently analyzed by CellQuest FACStation software. For staining of PBMCs or purified T and B cells, an identical approach was used, except for the lysing step. DNA-based ...
Prevention of gastrointestinal tumors based on adenomatous
Prevention of gastrointestinal tumors based on adenomatous

... tumors in animal models (22–25). In most cancers, however, TAAs are not identified. As an approach to load DCs with known or unidentified TAAs, DCs were pulsed with tumor lysates, transfected with tumor cell mRNA, or fused with tumor cells (26–28). Since human cancers develop as a result of the accu ...
Chimie de l`H érédité.
Chimie de l`H érédité.

... genetic   code.   Now,   however,   there   instead   exists   the   prevalent   belief   to which   I   also   subscribe   that   the   main   biochemical   devices   underlying   the transfer of genetic information to polypeptide chains are known. This happy state   of   affairs   is   in   large ...
Progressive divergence of definitive haematopoietic stem cells from
Progressive divergence of definitive haematopoietic stem cells from

... expressed KIT. However, in contrast to both the endothelial and haematopoietic lineages, this fraction was greatly enriched for KIThigh cells. Unlike the haematopoietic population, all endothelial and DP cells expressed low levels of SCA1. AA4.1 and CD34, also associated with the embryonic HSCs phen ...
Fulltext PDF - Indian Academy of Sciences
Fulltext PDF - Indian Academy of Sciences

... genes encoding fermentation enzymes from prokaryotes by means of lateral gene transfer (LGT) (Rosenthal et al 1997; Field et al 2000; Nixon et al 2002a). One of the most important conclusions of whole genome sequences of > 50 prokarytes of diverse types is that sets of genes encoding particular bioc ...
Thuy`s Rho family GTPases review
Thuy`s Rho family GTPases review

... Buchsbaum, R. (2007). Rho activation at a glance. Wittmann, T. and Storer, C. (2001). Cell motility: can Rho GTPases and microtubules point the way? Allen, W. et al. (1998). A role for Cdc42 in Macrophage chemotaxis. Sah, V. et al. (2000). The Role of Rho in G Protein-coupled receptor signal ...
univERsity oF copEnhAGEn
univERsity oF copEnhAGEn

... as the preferred method to visualize and analyse ion fluxes, signaling components, and metabolites, covering an expanding palette of cellular processes. While fluorescent proteins as such are mainly used for localization and expression studies, genetically encoded fluorescent biosensors in addition ...
Translocation of DNA across Bacterial Membranes
Translocation of DNA across Bacterial Membranes

... (153) proposed that the clustered gold label during immunoelectron microscopy derived from a continuous flow of the protein molecules from discrete secretion sites in the inner membrane. Since the periplasm has a gel-like consistency, the proteins do not diffuse through it but remain confined to a s ...
Posttranscriptional Regulation of the Heterochronic Gene lin
Posttranscriptional Regulation of the Heterochronic Gene lin

... site phenotypes (Ambros and Horvitz, 1987). lin-14(lf) alleles cause larvae stage 2 (L2) patterns of cell lineage in a variety of tissues to be executed precociously during the L1 stage (Ambros and Horvitz, 1987). Two lin-14(gf) alleles cause the opposite transformation in temporal cell fate, reiter ...
characterisation of amino acid transport in red blood cells of a
characterisation of amino acid transport in red blood cells of a

... sugars in addition to /3-amino acids. It is down-regulated by catecholamines and inhibited by loop diuretics (Wolowyk etal. 1989). Similar transport mechanisms are present in skate red cells (Goldstein and Kleinzeller, 1987). Recent studies implicate protein kinase C in the volume regulatory respons ...
Eph receptor signaling in C. elegans
Eph receptor signaling in C. elegans

... couples to the MAPK cascade is incompletely understood, but several downstream mediators have been identified. A genome-wide RNAi screen identified numerous genes that function in oocytes to inhibit MPK-1 phosphorylation (Govindan et al., 2006). Genetic tests are consistent with the model that the D ...
Actin Filaments of Guard Cells Are Reorganized
Actin Filaments of Guard Cells Are Reorganized

... The sample-handling procedures for labeling microtubules of leaf epidermis were very similar to those given for localization of actin filaments except for some differences during fixation and incubation with antibodies: epidermal tissue was fixed at room temperature with 3.7% paraformaldehyde, 0.2% ...
Specific amino acids in the BAR domain allow homodimerization
Specific amino acids in the BAR domain allow homodimerization

... Next, we analysed whether SNX33 is able to form heterodimers with other BAR domain-containing proteins, in particular with its homologues SNX9 and SNX18, but also with the more distantly related SNX1. As a positive control, SNX33 was used. All four proteins were transiently expressed as HA-tagged pr ...
Raven/Johnson Biology 8e Chapter 03
Raven/Johnson Biology 8e Chapter 03

... C. Answer c is incorrect. The linear sequence of the amino acids that makes up a protein is a critical property that determines the folding of the protein through the relative position of the various R-groups. Shape is a critical property that determines protein function, but it is not the only fact ...
Raven/Johnson Biology 8e
Raven/Johnson Biology 8e

... a. the primary level of protein structure b. the secondary level of protein structure c. the tertiary level of protein structure d. all of the above The correct answer is d— A. Answer a is incorrect. Although changing an amino acid does change the primary sequence, this is not the only effect. The c ...
Synaptic Regulation of the Light-Dependent Oscillatory Currents in
Synaptic Regulation of the Light-Dependent Oscillatory Currents in

... these findings, the presynaptic bipolar cells show calciumdependent oscillations of their membrane potential that leads to pulsatile release of transmitter and oscillatory activity of postsynaptic targets (Burrone and Lagnado 1997; Ma and Pan 2003). Interestingly, oscillations have also been reporte ...
Pausing of Golgi Bodies on Microtubules Regulates
Pausing of Golgi Bodies on Microtubules Regulates

... images (Figures 1P and 1Q). In addition to Golgi bodies, we identified a second, relatively rare population (<1 observed per cell per 10 min imaging period) of smaller, homogeneously labeled particles with the same rapid, nonlinear movement as Golgi bodies (Figures 1J and 1K). These particles were i ...
Strategy for Nonenveloped Virus Entry
Strategy for Nonenveloped Virus Entry

... partially uncoated, but still large (⬃70-nm), particle with active transcriptases for viral mRNA synthesis directly into the cytoplasm. This process is likely initiated by a particle form that resembles infectious subvirion particles (ISVPs), disassembly intermediates produced from virions by proteo ...
Myosin-Powered Membrane Compartment Drives Cytoplasmic
Myosin-Powered Membrane Compartment Drives Cytoplasmic

... microtubules and actin microfilaments with associated molecular motors [2–4]. Transport can be achieved by at least two mechanisms: active transport by receptor-mediated attachment of cargo to molecular motors and passive transport with cytosol flow or cytoplasmic streaming. It stands to reason that ...
Repression of viral transcription during herpes simplex virus latency
Repression of viral transcription during herpes simplex virus latency

... Rice et al., 1995). Once IE proteins have been produced in adequate amounts, the infected cell is usually committed to transcription of the remainder of the viral genome, followed by DNA replication and synthesis of progeny virions. Without IE proteins, productive infection cannot occur. Transcripti ...
Slide 1 - Biskit
Slide 1 - Biskit

... efficiently calculated from an exact and parameter-free mathematical model. VSO correlates very well with the protection of residues from itinerant water fluxes, as computed by Mihalek and colleagues (see above) which, in turn, can be considered a measure of residue activity. The calculation of shel ...
Purification, Identification and Characterisation of - DORAS
Purification, Identification and Characterisation of - DORAS

... Km determinations for Z-Gly-Pro-AMC A 300µM stock solution of Z-Gly-ProAMC in 5%v/v MeOH containing 500mM NaCl was prepared. A range of concentrations (0300µM) was prepared from this stock using 100mM potassium phosphate, pH 7.4, containing 5%v/v MeOH and 500mM NaCl as diluant. Purified ZIP was assa ...
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