EFFECT OF ASCORBIC ACID CONCENTRATION ON
... the cells. Double staining was used to assess dividing cells (figs 3A, B and C). Histochemical method was used to determine of ascorbic acid in the cells (figs. 4A, B, C and D). In survey about apical area in root of Aloe plants grown in medium without exogenous ascorbic acid and comparing them with ...
... the cells. Double staining was used to assess dividing cells (figs 3A, B and C). Histochemical method was used to determine of ascorbic acid in the cells (figs. 4A, B, C and D). In survey about apical area in root of Aloe plants grown in medium without exogenous ascorbic acid and comparing them with ...
Connection of the Mitochondrial Outer and Inner Membranes by
... the ⌬fzo1 deletion mutant which is rho0, that is, it lacks mitochondrial DNA (Rapaport et al., 1998). This strain was crossed with its isogenic wild-type strain YPH500 (Sikorski and Hieter, 1989). The resulting diploid strain was sporulated and tetrads were dissected. One rho⫹ clone was isolated tha ...
... the ⌬fzo1 deletion mutant which is rho0, that is, it lacks mitochondrial DNA (Rapaport et al., 1998). This strain was crossed with its isogenic wild-type strain YPH500 (Sikorski and Hieter, 1989). The resulting diploid strain was sporulated and tetrads were dissected. One rho⫹ clone was isolated tha ...
synthesis: role of the nsP2 protein. convert from minus
... 17) and encodes a nuclear localization signal (38). The phosphoprotein nsP3 is essential, although its role is unknown, for the replicase and transcriptase activities (25, 29, 36, 57). nsP4 contains the conserved GDD sequence motif found in RNA* Corresponding author. Phone: (419) 381-4337 or (419) 3 ...
... 17) and encodes a nuclear localization signal (38). The phosphoprotein nsP3 is essential, although its role is unknown, for the replicase and transcriptase activities (25, 29, 36, 57). nsP4 contains the conserved GDD sequence motif found in RNA* Corresponding author. Phone: (419) 381-4337 or (419) 3 ...
Human red blood cells-1 - Science Publishing Group
... based such as patch-clamp, flow cytometry or fluorescent lifetime cell imaging, because it enables to identify subpopulations of cells that can hardly be addressed by measurement of huge populations of cells such as tracer flux analysis [19, 20]. ...
... based such as patch-clamp, flow cytometry or fluorescent lifetime cell imaging, because it enables to identify subpopulations of cells that can hardly be addressed by measurement of huge populations of cells such as tracer flux analysis [19, 20]. ...
Transport of intravenously-injected ferritin across the guinea
... examined at each point in the time scale. Glutaraldehyde 3 per cent. was immediately injected intra-articularly into both knees to fix the synovium, which was then dissected off under the dissecting microscope; some was processed for light microscopy and stained with haematoxylin and eosin, Perls's ...
... examined at each point in the time scale. Glutaraldehyde 3 per cent. was immediately injected intra-articularly into both knees to fix the synovium, which was then dissected off under the dissecting microscope; some was processed for light microscopy and stained with haematoxylin and eosin, Perls's ...
Internal ribosome entry in the coding region of murine hepatitis virus
... Z5ab N, where the 5'-proximal and 5'-distal ORFs, ORF Z and ORF 5b/bl°/b N, are translated, whilst the internal ORF, ORF 5a, is translationally inactive. Clearly, the upstream ORF Z, provides an effective barrier to scanning ribosomes but does not prevent the initiation of ORF 5b translation. Our co ...
... Z5ab N, where the 5'-proximal and 5'-distal ORFs, ORF Z and ORF 5b/bl°/b N, are translated, whilst the internal ORF, ORF 5a, is translationally inactive. Clearly, the upstream ORF Z, provides an effective barrier to scanning ribosomes but does not prevent the initiation of ORF 5b translation. Our co ...
Six Distinct Nuclear Factors Interact with the 75-Base
... bound over the EcoRV site. Direct-binding studies with labeled fragments showed that this complex was formed on fragment VII but not on fragments IX or XI, again indicating that the site was disrupted by cleavage at EcoRV (data not shown). This correlates well with data generated by Schulze et al. ( ...
... bound over the EcoRV site. Direct-binding studies with labeled fragments showed that this complex was formed on fragment VII but not on fragments IX or XI, again indicating that the site was disrupted by cleavage at EcoRV (data not shown). This correlates well with data generated by Schulze et al. ( ...
Proteomic Approach to Identify Novel
... 25% in primary metabolism such as pyruvate decarboxylation, citric acid cycle, and amino acid and nucleotide metabolism. Five identified proteins represent chaperones; others are involved in molecular transport or protection against O2. Only two identified proteins form part of the mitochondrial pro ...
... 25% in primary metabolism such as pyruvate decarboxylation, citric acid cycle, and amino acid and nucleotide metabolism. Five identified proteins represent chaperones; others are involved in molecular transport or protection against O2. Only two identified proteins form part of the mitochondrial pro ...
WW Domains Provide a Platform for the
... Many signaling proteins contain modular domains that mediate specific protein-protein interactions, frequently through the recognition of short peptide motifs in their binding partners (56). In many cases these interactions are regulated by posttranslational modifications, such as phosphorylation. I ...
... Many signaling proteins contain modular domains that mediate specific protein-protein interactions, frequently through the recognition of short peptide motifs in their binding partners (56). In many cases these interactions are regulated by posttranslational modifications, such as phosphorylation. I ...
to Light and Abscisic Acid
... Department of Life Science (S.-O.E., Y.L.), School of Environmental Engineering (Y.L.), Pohang University of Science and Technology, Pohang, 790-784, Korea; and lnstitute of Molecular Biology, Academia Sinica, Nankang, Taipei, Taiwan 11 529, Republic of China (Y.L.) actin monomers and tubulin dimers ...
... Department of Life Science (S.-O.E., Y.L.), School of Environmental Engineering (Y.L.), Pohang University of Science and Technology, Pohang, 790-784, Korea; and lnstitute of Molecular Biology, Academia Sinica, Nankang, Taipei, Taiwan 11 529, Republic of China (Y.L.) actin monomers and tubulin dimers ...
Enhancers as non-coding RNA transcription units: recent
... eRNAs as signatures of functional enhancers? Annotation solely by epigenomic marks may not be the optimum way to define active enhancers. A recent examination of the high binding density or affinity of TFs and other chromatin regulators (that is, Mediator) defined a group of clustered enhancers in ...
... eRNAs as signatures of functional enhancers? Annotation solely by epigenomic marks may not be the optimum way to define active enhancers. A recent examination of the high binding density or affinity of TFs and other chromatin regulators (that is, Mediator) defined a group of clustered enhancers in ...
1 Electrophysiology and pacemaker function of the - AJP
... canine hearts the pacemaker cells are more concentrated in the proximity of the sinus node artery at the junction of the superior vena cava and the right atrium (12, 94, 99). Studies on the cytological organization of the SAN have revealed a structure composed of a dense array of connective tissue i ...
... canine hearts the pacemaker cells are more concentrated in the proximity of the sinus node artery at the junction of the superior vena cava and the right atrium (12, 94, 99). Studies on the cytological organization of the SAN have revealed a structure composed of a dense array of connective tissue i ...
The hypersensitive response is associated with host and nonhost
... showed HR lesions upon inoculation. In plants with high levels of partial resistance, such as S. berthaultii-11, a high percentage of unsuccessful (aborted) infections was found, but also a low percentage (16%) of slowly growing lesions (1.2 mm/day). Plants with lower levels of partial resistance, s ...
... showed HR lesions upon inoculation. In plants with high levels of partial resistance, such as S. berthaultii-11, a high percentage of unsuccessful (aborted) infections was found, but also a low percentage (16%) of slowly growing lesions (1.2 mm/day). Plants with lower levels of partial resistance, s ...
Primary endothelial cells isolated from the yolk sac
... numbers of macrophage and megakaryocyte progenitors.2 On 8.25 dpc, definitive progenitor cells also emerge in the yolk sac but not the embryo.3,4 By 8.5 dpc, the frequency of EryP diminishes and EryP are absent in the 9.0-dpc yolk sac; however, the frequency of definitive progenitor cells increases ...
... numbers of macrophage and megakaryocyte progenitors.2 On 8.25 dpc, definitive progenitor cells also emerge in the yolk sac but not the embryo.3,4 By 8.5 dpc, the frequency of EryP diminishes and EryP are absent in the 9.0-dpc yolk sac; however, the frequency of definitive progenitor cells increases ...
biotechnology for the production of plant natural products
... the target cell or tissue, which is subsequently in vitro cultured, and regenerated to produce mature transformed plants. The particles are usually of gold or tungsten nature, of small size (0.5-5 μm) but with enough mass to be accelerated and able to penetrate the target cell or tissue carrying the ...
... the target cell or tissue, which is subsequently in vitro cultured, and regenerated to produce mature transformed plants. The particles are usually of gold or tungsten nature, of small size (0.5-5 μm) but with enough mass to be accelerated and able to penetrate the target cell or tissue carrying the ...
A Novel Recombinant Plasma Membrane
... The schematic structure of the novel ATP probe (heretofore referred to as pmeLUC) is shown in Figure 1A. This chimeric protein, thanks to the folate receptor leader sequence, is targeted to the plasma membrane and detects ATP in the extracellular milieu close to the cell surface (Figure 1B). Immunof ...
... The schematic structure of the novel ATP probe (heretofore referred to as pmeLUC) is shown in Figure 1A. This chimeric protein, thanks to the folate receptor leader sequence, is targeted to the plasma membrane and detects ATP in the extracellular milieu close to the cell surface (Figure 1B). Immunof ...
The constant beat: cardiomyocytes adapt their forces by equal
... case sarcomere alignment was reduced in comparison to the former. Additionally, cellular forces were reported to be highest on 10 kPa substrates and reduced on softer and stiffer materials (Jacot et al., 2008; Jacot et al., 2010). For neonatal cardiomyocytes grown on elastomeric micropillars, twitch ...
... case sarcomere alignment was reduced in comparison to the former. Additionally, cellular forces were reported to be highest on 10 kPa substrates and reduced on softer and stiffer materials (Jacot et al., 2008; Jacot et al., 2010). For neonatal cardiomyocytes grown on elastomeric micropillars, twitch ...
Molecular Identification and Physiological
... connecting transmembrane domains 6 and 7 (Figure 1). This loop spans ;320 amino acid residues in length, which is nearly four to five times larger than the corresponding structures in all other known monosaccharide transporters from prokaryotes and eukaryotes, and is not present in the Synechocystis ...
... connecting transmembrane domains 6 and 7 (Figure 1). This loop spans ;320 amino acid residues in length, which is nearly four to five times larger than the corresponding structures in all other known monosaccharide transporters from prokaryotes and eukaryotes, and is not present in the Synechocystis ...
Review THE SELECTION OF APTAMERS SPECIFIC FOR
... applications, e.g. in targeted drug delivery. Recent papers have contained some fragmentary review information on aptamers with membrane molecular targets ...
... applications, e.g. in targeted drug delivery. Recent papers have contained some fragmentary review information on aptamers with membrane molecular targets ...
Knockdown of zebrafish hoxb1 genes - Development
... infer the mechanisms that have facilitated retention of specific Hox gene duplicates in the zebrafish. Gene duplication has long been thought to play an important role in evolution by providing new genetic material for selection to act upon (Ohno, 1970). Classical models have assumed that after a du ...
... infer the mechanisms that have facilitated retention of specific Hox gene duplicates in the zebrafish. Gene duplication has long been thought to play an important role in evolution by providing new genetic material for selection to act upon (Ohno, 1970). Classical models have assumed that after a du ...
Trafficking of the human transferrin receptor in plant cells: effects of
... an Alexa 488-labelled secondary antibody. Samples were then analysed by confocal microscopy. As shown in Figure 2, hTfR was found mainly in vesicular structures distributed throughout the cytoplasm, and only partially at the plasma membrane. To test for cell surface expression of the receptor and fo ...
... an Alexa 488-labelled secondary antibody. Samples were then analysed by confocal microscopy. As shown in Figure 2, hTfR was found mainly in vesicular structures distributed throughout the cytoplasm, and only partially at the plasma membrane. To test for cell surface expression of the receptor and fo ...
the 36th NPS abstract book
... Arbuscular mycorrhizas (AM) are symbiotic associations involving up to 80% of terrestrial plants and symbiotic fungi belonging to Glomeromycota. By colonizing the root through the development of inter- and intracellular hyphae and the formation of the highly branched structures, called arbuscules, A ...
... Arbuscular mycorrhizas (AM) are symbiotic associations involving up to 80% of terrestrial plants and symbiotic fungi belonging to Glomeromycota. By colonizing the root through the development of inter- and intracellular hyphae and the formation of the highly branched structures, called arbuscules, A ...
Phospholipase D promotes Arcanobacterium haemolyticum
... mammalian plasma membrane [17]. SM, with cholesterol and GPI-anchored proteins, predominantly partitions to lipid rafts, which are tightly packed, membrane micro-domains that act to compartmentalize cellular processes on the outer leaflet of the plasma membrane [18]. Lipid rafts are also implicated ...
... mammalian plasma membrane [17]. SM, with cholesterol and GPI-anchored proteins, predominantly partitions to lipid rafts, which are tightly packed, membrane micro-domains that act to compartmentalize cellular processes on the outer leaflet of the plasma membrane [18]. Lipid rafts are also implicated ...
New immunotherapies targeting the
... changes at the PD-1 locus [27]. Other epigenetic modifications have been described including regulation of PD-1 by DNA methylation. Viral infection leads to a loss of this methylation in CD8 T cells which then allows transcription of PD-1 [26,33]. This demethylation is directly related to the streng ...
... changes at the PD-1 locus [27]. Other epigenetic modifications have been described including regulation of PD-1 by DNA methylation. Viral infection leads to a loss of this methylation in CD8 T cells which then allows transcription of PD-1 [26,33]. This demethylation is directly related to the streng ...
Chromoplast Differentiation: Current Status and
... plastoglubuli containing small amounts of carotenoids are also present, although in small size and number (Kim et al. 2010). Mango fruit chromoplasts contain large and numerous globules as well as a network of tubular membranes. They can be considered as both the globular and reticulotubular types ( ...
... plastoglubuli containing small amounts of carotenoids are also present, although in small size and number (Kim et al. 2010). Mango fruit chromoplasts contain large and numerous globules as well as a network of tubular membranes. They can be considered as both the globular and reticulotubular types ( ...