salinity change and cell volume: the response of tissues from the
... Gill, mantle and ventricle tissues were prepared for amino acid or betaine analysis by extraction with perchloric acid (PCA). Tissue pieces were homogenized in distilled water and allowed to extract overnight at 4 °C in 6 % PCA. Samples were then centrifuged at 50 000 g for 20 min. The supernatant w ...
... Gill, mantle and ventricle tissues were prepared for amino acid or betaine analysis by extraction with perchloric acid (PCA). Tissue pieces were homogenized in distilled water and allowed to extract overnight at 4 °C in 6 % PCA. Samples were then centrifuged at 50 000 g for 20 min. The supernatant w ...
thebacterialflagellum
... the conformational change between these forms are relatively small. To study the nature of the conformational change Namba et al. used a model of three consecutive protofilament subunits. The top subunit was fixed and then the lower subunit was pulled down to see what would happen in the middle subu ...
... the conformational change between these forms are relatively small. To study the nature of the conformational change Namba et al. used a model of three consecutive protofilament subunits. The top subunit was fixed and then the lower subunit was pulled down to see what would happen in the middle subu ...
The Second Exon-Encoded Factor XII Region Is Involved in the
... percentage of the total counts added. Under these experimental conditions, no significant binding of labeled proteins to the tubes (less than 0.5%) was observed. The efficiency of the inhibition of the binding was analyzed by computing the regression line of the binding curve and establishing the am ...
... percentage of the total counts added. Under these experimental conditions, no significant binding of labeled proteins to the tubes (less than 0.5%) was observed. The efficiency of the inhibition of the binding was analyzed by computing the regression line of the binding curve and establishing the am ...
Avoidance of Four-way Junctions and
... Figure 2. Antitubulin labeling of isodiametric, premitotic epidermal cells. (a and b) In the upper cell in a, cortical MTs radiate from several points of intense fluorescence (e.g., black arrows). These can be traced in b (e.g., white arrow) to strands radiating from the nucleus. In the lower cell ...
... Figure 2. Antitubulin labeling of isodiametric, premitotic epidermal cells. (a and b) In the upper cell in a, cortical MTs radiate from several points of intense fluorescence (e.g., black arrows). These can be traced in b (e.g., white arrow) to strands radiating from the nucleus. In the lower cell ...
- Wiley Online Library
... probably also the case for plants (Kim et al., 2001; Vermeer et al., 2006). PtdIns4P is the most abundant isomer, making up approximately 80% of the plant PtdInsP pool (Meijer and Munnik, 2003; Meijer et al., 2001; Munnik et al., 1994b). In addition to being important for phospholipase C (PLC) signa ...
... probably also the case for plants (Kim et al., 2001; Vermeer et al., 2006). PtdIns4P is the most abundant isomer, making up approximately 80% of the plant PtdInsP pool (Meijer and Munnik, 2003; Meijer et al., 2001; Munnik et al., 1994b). In addition to being important for phospholipase C (PLC) signa ...
Informatics approaches to understanding TGFb pathway regulation
... We describe how to identify related sequences, how to create an alignment of the sequences, how to generate a tree from the alignment and how to incorporate statistics. BLAST is a program available at the NCBI website that identifies related sequences (Johnson et al., 2008) (http://blast. ncbi.nlm.n ...
... We describe how to identify related sequences, how to create an alignment of the sequences, how to generate a tree from the alignment and how to incorporate statistics. BLAST is a program available at the NCBI website that identifies related sequences (Johnson et al., 2008) (http://blast. ncbi.nlm.n ...
Danon disease - Journal of Cell Science
... cognitive defects and visual problems. Although individuals with Danon disease have been clinically described since the early 1980s, the underlying molecular mechanisms involved in pathological progression remain poorly understood. LAMP-2 is known to be involved in autophagy, and a characteristic ac ...
... cognitive defects and visual problems. Although individuals with Danon disease have been clinically described since the early 1980s, the underlying molecular mechanisms involved in pathological progression remain poorly understood. LAMP-2 is known to be involved in autophagy, and a characteristic ac ...
Doehrty-McMahon 2009
... all invaginated CCPs, and other membrane invaginations observed by this technique, subsequently undergo scission from the plasma membrane. Only with the development of total internal reflection fluorescence microscopy (TIR-FM), which allows the visualization in real time of the behavior of fluorescent ...
... all invaginated CCPs, and other membrane invaginations observed by this technique, subsequently undergo scission from the plasma membrane. Only with the development of total internal reflection fluorescence microscopy (TIR-FM), which allows the visualization in real time of the behavior of fluorescent ...
Lesson Prepared Under MHRD project “National Mission on
... Parenchyma cells may be elongated columnar in shape as found in palisade tissue of the leaf. Stems of plants shows well-developed air spaces (Scirpus, petiole of Canna leaf) with their stretched arms, such parenchymatous cells are called stellate parenchyma. Parenchyma cells can be variously lobed e ...
... Parenchyma cells may be elongated columnar in shape as found in palisade tissue of the leaf. Stems of plants shows well-developed air spaces (Scirpus, petiole of Canna leaf) with their stretched arms, such parenchymatous cells are called stellate parenchyma. Parenchyma cells can be variously lobed e ...
PHYSIOLOGICAL FUNCTIONS OF CLC Cl − CHANNELS
... myotonic goat strain. Myotonia, a symptom found in several genetic diseases, is an impairment of skeletal muscle relaxation after voluntary contraction. It results from an increase in muscle excitability that can be detected in electromyograms in the form of myotonic runs, i.e., long trains of actio ...
... myotonic goat strain. Myotonia, a symptom found in several genetic diseases, is an impairment of skeletal muscle relaxation after voluntary contraction. It results from an increase in muscle excitability that can be detected in electromyograms in the form of myotonic runs, i.e., long trains of actio ...
Identification and developmental expression of the Xenopus laevis
... PCR analysis. Total RNA samples were obtained from 11 different developmental stages of Xenopus embryos (19). cDNA copies of these RNA samples were prepared by reverse transcription with random oligonucleotide priming. High stringency PCR, using primers to the uniformly expressed ornithine decarboxy ...
... PCR analysis. Total RNA samples were obtained from 11 different developmental stages of Xenopus embryos (19). cDNA copies of these RNA samples were prepared by reverse transcription with random oligonucleotide priming. High stringency PCR, using primers to the uniformly expressed ornithine decarboxy ...
Efficient high light acclimation involves rapid
... response mechanisms that allow efficient acclimation. Most signals originate from the chloroplast itself. In addition to very fast photochemical regulation, intensive molecular communication is realized within the photosynthesizing cell, optimizing the acclimation process. Current research has opene ...
... response mechanisms that allow efficient acclimation. Most signals originate from the chloroplast itself. In addition to very fast photochemical regulation, intensive molecular communication is realized within the photosynthesizing cell, optimizing the acclimation process. Current research has opene ...
The Calyptogena magnifica Chemoautotrophic Symbiont Genome
... identify their potential in vivo targets, we examined effects of OspF on the host immune signaling pathways, including those of nuclear factor kB (NFkB) and MAPKs. Transient expression of OspF in human 293T cells efficiently blocked extracellular signal– ...
... identify their potential in vivo targets, we examined effects of OspF on the host immune signaling pathways, including those of nuclear factor kB (NFkB) and MAPKs. Transient expression of OspF in human 293T cells efficiently blocked extracellular signal– ...
Implications for AIDS Simian Immunodeficiency Virus Infection of
... In contrast to HIV-infected humans, naturally SIV-infected sooty mangabeys (SMs) very rarely progress to AIDS. Although the mechanisms underlying this disease resistance are unknown, a consistent feature of natural SIV infection is the absence of the generalized immune activation associated with HIV ...
... In contrast to HIV-infected humans, naturally SIV-infected sooty mangabeys (SMs) very rarely progress to AIDS. Although the mechanisms underlying this disease resistance are unknown, a consistent feature of natural SIV infection is the absence of the generalized immune activation associated with HIV ...
Starting the protein synthesis machine: eukaryotic
... proteins (indicated above the schematic protein sequence). Most of these interaction sites have conserved amino acid and/or structural signatures (named below the eIF4G schematic: MA3, first identified in the mouse MA3 protein; MIF4G, middle portion of eIF4G; W2, named after two conserved tryptophan ...
... proteins (indicated above the schematic protein sequence). Most of these interaction sites have conserved amino acid and/or structural signatures (named below the eIF4G schematic: MA3, first identified in the mouse MA3 protein; MIF4G, middle portion of eIF4G; W2, named after two conserved tryptophan ...
Metabolism of Methanol in Plant Cells. Carbon-13
... C-NMR spectra of perchloric acid extracts obtained from sycamore cells maintained at pH 6.0 for 7 d in a nutrient medium containing 5 mm [13C]methanol. In the 13C-NMR reference spectra (in which methanol was omitted from the nutrient solution), the strongest signals were from glucosyl and fructosyl ...
... C-NMR spectra of perchloric acid extracts obtained from sycamore cells maintained at pH 6.0 for 7 d in a nutrient medium containing 5 mm [13C]methanol. In the 13C-NMR reference spectra (in which methanol was omitted from the nutrient solution), the strongest signals were from glucosyl and fructosyl ...
Locus-Specific Control of DNA Methylation by the
... mail.jhmi.edu; fax 410-955-2926. The author responsible for distribution of materials integral to the findings presented in this article in accordance with the policy described in the Instructions for Authors (www.plantcell.org) is: Judith Bender ([email protected]). W Online version contains We ...
... mail.jhmi.edu; fax 410-955-2926. The author responsible for distribution of materials integral to the findings presented in this article in accordance with the policy described in the Instructions for Authors (www.plantcell.org) is: Judith Bender ([email protected]). W Online version contains We ...
Role of cholesterol in SNARE-mediated trafficking on intracellular
... (LE/MVBs), where CEs are hydrolyzed. Free cholesterol is then distributed to other sites (see below), whereas the LDL particle is degraded in lysosomes (Lys) (3). In addition, recycling of cholesterol from EEs, SEs and REs (4) or the LE/MVB (5) to the plasma membrane, or its retrograde transport fro ...
... (LE/MVBs), where CEs are hydrolyzed. Free cholesterol is then distributed to other sites (see below), whereas the LDL particle is degraded in lysosomes (Lys) (3). In addition, recycling of cholesterol from EEs, SEs and REs (4) or the LE/MVB (5) to the plasma membrane, or its retrograde transport fro ...
Word - The Open University
... Actin is a highly conserved protein. Most organisms have several genes encoding actin; in humans there are six principal isoforms, four of which are found in different types of muscle and the other two (β and γ) in all non-muscle cells. (The term ‘isoform’ describes variants of a protein. These may ...
... Actin is a highly conserved protein. Most organisms have several genes encoding actin; in humans there are six principal isoforms, four of which are found in different types of muscle and the other two (β and γ) in all non-muscle cells. (The term ‘isoform’ describes variants of a protein. These may ...
Nucleolytic processing of aberrant replication intermediates by an
... force of malignant transformation (4,21). Oncogene activation causes replication fork stalling that leads to overreplication, fork collapse and DNA breaks formation (22– 24). In pre-cancerous lesions, the DNA damage response is thought to act as a barrier to tumor progression by driving cells with u ...
... force of malignant transformation (4,21). Oncogene activation causes replication fork stalling that leads to overreplication, fork collapse and DNA breaks formation (22– 24). In pre-cancerous lesions, the DNA damage response is thought to act as a barrier to tumor progression by driving cells with u ...
figure 1 - Proceedings of the Royal Society B
... different cellular processes remains poorly understood. By examining the elemental composition of major functional classes of proteins in four multicellular eukaryotic model organisms, we find that the catabolic machinery shows substantially lower N content than the anabolic machinery and the rest o ...
... different cellular processes remains poorly understood. By examining the elemental composition of major functional classes of proteins in four multicellular eukaryotic model organisms, we find that the catabolic machinery shows substantially lower N content than the anabolic machinery and the rest o ...
Defects in mesoderm, neural tube and vascular development in
... fragment including the FN 5 ′ exon and first three FN type I repeat exons was subcloned into Bluescript SK- (Stratagene). The neo/ G418-resistance gene, under control of the mouse PGK-1 promoter and PGK poly(A) addition site (McBurney et al., 1991), was isolated from plasmid pKJ1 by digestion with E ...
... fragment including the FN 5 ′ exon and first three FN type I repeat exons was subcloned into Bluescript SK- (Stratagene). The neo/ G418-resistance gene, under control of the mouse PGK-1 promoter and PGK poly(A) addition site (McBurney et al., 1991), was isolated from plasmid pKJ1 by digestion with E ...
Signatures of nitrogen limitation in the elemental composition of the
... different cellular processes remains poorly understood. By examining the elemental composition of major functional classes of proteins in four multicellular eukaryotic model organisms, we find that the catabolic machinery shows substantially lower N content than the anabolic machinery and the rest o ...
... different cellular processes remains poorly understood. By examining the elemental composition of major functional classes of proteins in four multicellular eukaryotic model organisms, we find that the catabolic machinery shows substantially lower N content than the anabolic machinery and the rest o ...
Reprogramming the Genetic Code: From Triplet to Quadruplet Codes
... may be poorly accommodated in the ribosome and efforts to increase the efficiency of quadruplet decoding in natural translation by extended anticodon tRNAs may lead to missynthesis of the proteome, toxicity, and cell death. A solution to the efficient decoding of quadruplet codons comes from our wor ...
... may be poorly accommodated in the ribosome and efforts to increase the efficiency of quadruplet decoding in natural translation by extended anticodon tRNAs may lead to missynthesis of the proteome, toxicity, and cell death. A solution to the efficient decoding of quadruplet codons comes from our wor ...