Paramecium trichocysts isolated with their membranes are stable in
... fraction of Paramecium secretory vesicles consisting of both crystalline contents and limiting membrane. Our primary motivation in isolating intact vesicles was to develop a biochemical approach to the characterization of the products of the genes involved in the different steps of the secretory pat ...
... fraction of Paramecium secretory vesicles consisting of both crystalline contents and limiting membrane. Our primary motivation in isolating intact vesicles was to develop a biochemical approach to the characterization of the products of the genes involved in the different steps of the secretory pat ...
Penium margaritaceum: A Unicellular Model Organism for
... The utilization of new research methods and strategies emerging from technologies in molecular genetics, immuno-binding/cytochemical labeling, high resolution microscopy and spectroscopy have recently provided significant insight into deciphering the complexities of the plant cell wall [1–6]. The cu ...
... The utilization of new research methods and strategies emerging from technologies in molecular genetics, immuno-binding/cytochemical labeling, high resolution microscopy and spectroscopy have recently provided significant insight into deciphering the complexities of the plant cell wall [1–6]. The cu ...
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... The utilization of new research methods and strategies emerging from technologies in molecular genetics, immuno-binding/cytochemical labeling, high resolution microscopy and spectroscopy have recently provided significant insight into deciphering the complexities of the plant cell wall [1–6]. The cu ...
... The utilization of new research methods and strategies emerging from technologies in molecular genetics, immuno-binding/cytochemical labeling, high resolution microscopy and spectroscopy have recently provided significant insight into deciphering the complexities of the plant cell wall [1–6]. The cu ...
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... clones of early arising blast cells contributing to anterior segments and later born clones to more posterior segments (Figs. 1B, C). Previous work indicated that some early progeny of the M teloblasts make anterior non-segmental contributions (Zackson, 1982; Gleizer and Stent, 1993; Gline et al., 2 ...
... clones of early arising blast cells contributing to anterior segments and later born clones to more posterior segments (Figs. 1B, C). Previous work indicated that some early progeny of the M teloblasts make anterior non-segmental contributions (Zackson, 1982; Gleizer and Stent, 1993; Gline et al., 2 ...
How and why cells grow as rods Open Access Fred Chang
... micron-scale cells of specific shapes and sizes remains an outstanding question in biology. How are cell shapes generated? Are there reasons why cells have adopted certain shapes over others? Although many shape-determining factors have been identified across divergent organisms, mere characterizati ...
... micron-scale cells of specific shapes and sizes remains an outstanding question in biology. How are cell shapes generated? Are there reasons why cells have adopted certain shapes over others? Although many shape-determining factors have been identified across divergent organisms, mere characterizati ...
RNA interference screening in Drosophila primary cells for genes
... Fig. 1. Myogenesis in primary cultures derived from Drosophila embryos. (A-F) Fluorescence micrographs of freshly dissociated cells obtained from Drosophila gastrulating embryos carrying rp298-lacZ immediately following plating. Cells are stained using DAPI for nuclei (A, and blue in C), and antibod ...
... Fig. 1. Myogenesis in primary cultures derived from Drosophila embryos. (A-F) Fluorescence micrographs of freshly dissociated cells obtained from Drosophila gastrulating embryos carrying rp298-lacZ immediately following plating. Cells are stained using DAPI for nuclei (A, and blue in C), and antibod ...
Unique Eomes+ NK Cell Subsets Are Present in Uterus
... ILC1. Here, we show that both in humans and mice the majority of group 1 ILC in endometrium/uterus and decidua express Eomesodermin (Eomes), thus suggesting that they are developmentally related to conventional NK cells. However, they differ from peripheral NK cells. In humans, Eomes+ decidual NK (d ...
... ILC1. Here, we show that both in humans and mice the majority of group 1 ILC in endometrium/uterus and decidua express Eomesodermin (Eomes), thus suggesting that they are developmentally related to conventional NK cells. However, they differ from peripheral NK cells. In humans, Eomes+ decidual NK (d ...
Biology: Cells the Fundamental Unit of Life
... basic components of cells. It uses several instructional approaches that are meant to engage students in the process of understanding this complex and exciting material. In order to understand the basic functions of human life, students need to understand how cells work, how basic cells function, an ...
... basic components of cells. It uses several instructional approaches that are meant to engage students in the process of understanding this complex and exciting material. In order to understand the basic functions of human life, students need to understand how cells work, how basic cells function, an ...
Efficient and simple electro-transformation of intact cells for the
... in resistance resulted in an increase in the number of hygromycin-tolerant colonies. With 800 Ω resistance ...
... in resistance resulted in an increase in the number of hygromycin-tolerant colonies. With 800 Ω resistance ...
Global regulator H-NS and lipoprotein NlpI influence production of
... Hha is a global regulator [30] with nonspecific DNA binding [31] that alters the production of multiple proteins [32] and which forms a complex with H-NS that binds DNA [31]. To evaluate if the H-NS regulation of eDNA occurs through its interaction with Hha, we evaluated via qPCR the eDNA produced by ...
... Hha is a global regulator [30] with nonspecific DNA binding [31] that alters the production of multiple proteins [32] and which forms a complex with H-NS that binds DNA [31]. To evaluate if the H-NS regulation of eDNA occurs through its interaction with Hha, we evaluated via qPCR the eDNA produced by ...
DNA polymerase alpha, a component of the replication initiation
... Cells deleted for polymerase alpha undergo defective mitosis To investigate the role of DNA polymerase alpha in the checkpoint operating in S-phase we investigated the phenotype of germinating spores that had been disrupted for the pol1 gene by insertion of a single copy of the ura4 gene (Singh and ...
... Cells deleted for polymerase alpha undergo defective mitosis To investigate the role of DNA polymerase alpha in the checkpoint operating in S-phase we investigated the phenotype of germinating spores that had been disrupted for the pol1 gene by insertion of a single copy of the ura4 gene (Singh and ...
BIOSYNTHESIS IN ISOLATED ACETABULARIA CHLOROPLASTS I
... for periods of 6-8 hr, tracer was found in essentially all amino acid species of their hydrolyzed protein Phenylalanine labeling was not detected, probably due to technical problems, and hydroxyproline labeling was not tested for The incorporation of 14CO2 into the amino acids is driven by light and ...
... for periods of 6-8 hr, tracer was found in essentially all amino acid species of their hydrolyzed protein Phenylalanine labeling was not detected, probably due to technical problems, and hydroxyproline labeling was not tested for The incorporation of 14CO2 into the amino acids is driven by light and ...
Hydrocortisone perturbs the cell proliferation pattern during feather
... decreased in the interplumar areas (compare Rg. 3F and Rg. 3B-D). Serial longitudinal sections showed that more developed rudiments displayed features of E 6.5 feather buds, i.e., unlabeled epidermal placodes and labeled aggregating dermal cells (Fig. 4C). Both low and high magnifications corroborat ...
... decreased in the interplumar areas (compare Rg. 3F and Rg. 3B-D). Serial longitudinal sections showed that more developed rudiments displayed features of E 6.5 feather buds, i.e., unlabeled epidermal placodes and labeled aggregating dermal cells (Fig. 4C). Both low and high magnifications corroborat ...
Oriented Asymmetric Divisions That Generate the
... area) were not observed to divide and were scored as pavement cells. These cells were often shaped like jigsaw puzzle pieces with wavy margins. Smaller, less sinuous cells displayed three different behaviors (Figure 1, i to iii, top left). Most differentiated into pavement cells; some divided symmet ...
... area) were not observed to divide and were scored as pavement cells. These cells were often shaped like jigsaw puzzle pieces with wavy margins. Smaller, less sinuous cells displayed three different behaviors (Figure 1, i to iii, top left). Most differentiated into pavement cells; some divided symmet ...
laboratory science
... a bandwidth of 60 nm, 8 mW/cm2) RPE that had previously accumulated A2E underwent marked cell death. In the present experiments, 41.1% ⫾ 4.1% (3 experiments) of the A2E-laden cells in a field of illumination became nonviable after blue light exposure in the absence of an IOL (Figure 1). When the yel ...
... a bandwidth of 60 nm, 8 mW/cm2) RPE that had previously accumulated A2E underwent marked cell death. In the present experiments, 41.1% ⫾ 4.1% (3 experiments) of the A2E-laden cells in a field of illumination became nonviable after blue light exposure in the absence of an IOL (Figure 1). When the yel ...
The Hydra polyp: Nothing but an active stem cell community
... 1996). In 2000, pioneering studies in germinal development in Drosophila uncovered the niche structure in vivo for the first time and showed that stem cell populations are established in such ‘niches’(Xie & Spradling 2000). Subsequently, stem cell niches have been located and characterized in a numb ...
... 1996). In 2000, pioneering studies in germinal development in Drosophila uncovered the niche structure in vivo for the first time and showed that stem cell populations are established in such ‘niches’(Xie & Spradling 2000). Subsequently, stem cell niches have been located and characterized in a numb ...
bioinformatics - Noble Research Lab
... These methods include the yeast two-hybrid screen and methods based on mass spectrometry (see von Mering et al., 2002 and references therein). The data obtained by these methods are partial: each experimental assay can identify only a subset of the interactions, and it has been estimated that for th ...
... These methods include the yeast two-hybrid screen and methods based on mass spectrometry (see von Mering et al., 2002 and references therein). The data obtained by these methods are partial: each experimental assay can identify only a subset of the interactions, and it has been estimated that for th ...
Super-resolution microscopy of mitochondria
... throughout the mitochondrial network. In humans, the mtDNA encodes 13 proteins, which are essential for the function of OXPHOS. An important and still not conclusively answered question is whether a single nucleoid contains one or multiple mtDNA copies [57]. This issue has been addressed by a number ...
... throughout the mitochondrial network. In humans, the mtDNA encodes 13 proteins, which are essential for the function of OXPHOS. An important and still not conclusively answered question is whether a single nucleoid contains one or multiple mtDNA copies [57]. This issue has been addressed by a number ...
Cell death in Leishmania induced by stress and differentiation
... forms, similar morphological features of apoptosis could be induced, in vitro, with concanavalin A treatment,24,25 or under oxidative stress.26 Additionally, Leishmania amazonensis promastigotes display DNA laddering and chromatin condensation in dense clusters (leading to the breakdown of the nucle ...
... forms, similar morphological features of apoptosis could be induced, in vitro, with concanavalin A treatment,24,25 or under oxidative stress.26 Additionally, Leishmania amazonensis promastigotes display DNA laddering and chromatin condensation in dense clusters (leading to the breakdown of the nucle ...
Action Potential Transfer in Cell Pairs Isolated From Adult Rat and
... electrical properties and the topology of cell arrangements (e.g., see Fozzard1)- A change in each of these parameters is expected to modify the conduction velocity in the heart. In fact, the influence of membrane excitability on conduction velocity has long been recognized, while the importance of ...
... electrical properties and the topology of cell arrangements (e.g., see Fozzard1)- A change in each of these parameters is expected to modify the conduction velocity in the heart. In fact, the influence of membrane excitability on conduction velocity has long been recognized, while the importance of ...
NIH Public Access
... The decision for progenitors to differentiate is made at the G1 phase of the cell cycle where Dtype cyclins and cyclin-dependent kinases inhibitors (CKIs) play an important role in regulating cell cycle exit. The CKIs regulate cell cycle progression by blocking phosphorylation of the retinoblastoma ...
... The decision for progenitors to differentiate is made at the G1 phase of the cell cycle where Dtype cyclins and cyclin-dependent kinases inhibitors (CKIs) play an important role in regulating cell cycle exit. The CKIs regulate cell cycle progression by blocking phosphorylation of the retinoblastoma ...
The Mechanisms of hsp27 Antibody-Mediated Apoptosis in Retinal
... (Fig. 1 A). Using control mouse antibodies, anti-IgG or anticalbindin-D, immunogold labeling was negative in retinal neuronal cells (Fig. 1 B,C). The internalization of exogenously applied hsp27 antibody exhibited a time-dependent pattern. In retina incubated with hsp27 antibody for 30 min, immunogo ...
... (Fig. 1 A). Using control mouse antibodies, anti-IgG or anticalbindin-D, immunogold labeling was negative in retinal neuronal cells (Fig. 1 B,C). The internalization of exogenously applied hsp27 antibody exhibited a time-dependent pattern. In retina incubated with hsp27 antibody for 30 min, immunogo ...
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... B) Glucose entering the cell down its concentration gradient provides energy for uptake of sodium ions against the electrochemical gradient. C) Sodium ions can move down their electrochemical gradient through the cotransporter whether or not glucose is present outside the cell. D) The cotransporter ...
... B) Glucose entering the cell down its concentration gradient provides energy for uptake of sodium ions against the electrochemical gradient. C) Sodium ions can move down their electrochemical gradient through the cotransporter whether or not glucose is present outside the cell. D) The cotransporter ...
Chapter 4 Test Cell Structure Answer Section
... d. All of the above 32. The organelles associated with photosynthesis are the a. Golgi apparatus. b. vacuoles. c. mitochondria. d. chloroplasts. 33. In which of the following organelles is a cell’s ATP (energy) produced? a. Golgi apparatus b. endoplasmic reticulum c. mitochondrion d. lysosome 34. On ...
... d. All of the above 32. The organelles associated with photosynthesis are the a. Golgi apparatus. b. vacuoles. c. mitochondria. d. chloroplasts. 33. In which of the following organelles is a cell’s ATP (energy) produced? a. Golgi apparatus b. endoplasmic reticulum c. mitochondrion d. lysosome 34. On ...
unresponsive to cell division control by polypeptide mating hormone
... Polypeptide hormones are produced by each of the two mating types, a-factor by MA Th cells (1) and a-factor by MA Ta cells (29); these hormones arrest division in the cell of opposite mating type at start (3, 31) and, together, they synchronize the cell cycles of the two partners in conjugation (10) ...
... Polypeptide hormones are produced by each of the two mating types, a-factor by MA Th cells (1) and a-factor by MA Ta cells (29); these hormones arrest division in the cell of opposite mating type at start (3, 31) and, together, they synchronize the cell cycles of the two partners in conjugation (10) ...