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Further analysis on lidocaine-induced increase in intracellular Zn2+
Further analysis on lidocaine-induced increase in intracellular Zn2+

... The methods for measuring the cellular and membrane parameters by using a flow cytometer equipped with an argon laser (CytoACE-150; JASCO, Tokyo, Japan) and fluorescent probes were similar to those described previously (Chikahisa et al., 1996; Matsui et al., 2008). The fluorescence was analyzed by J ...
Interactions of Annexins with the mu Subunits of the Clathrin
Interactions of Annexins with the mu Subunits of the Clathrin

... whether these motifs in other annexins might also support an interaction with mu2 we subcloned the N-terminal domains of annexins A1, A6, A7, and A11, as well as the intervening domain of annexin A6, as indicated in Figure 1, into the bait vector for the two-hybrid screen. However, no ...
Four-cell stage mouse blastomeres have different developmental
Four-cell stage mouse blastomeres have different developmental

... Since in a major group of embryos (ME embryos) the progeny of individual four-cell blastomeres tends to follow different fates, the question arises as to whether they are equivalent to each other. This could happen, for example, as a consequence of their spatial relationship per se or because they i ...
Upregulation of Alveolar Epithelial Active Na Transport Is
Upregulation of Alveolar Epithelial Active Na Transport Is

Fibronectin and Other Adhesive Glycoproteins
Fibronectin and Other Adhesive Glycoproteins

... The FN1 module is found only in chordates (Tucker and Chiquet-Ehrismann 2009) (see Chap. 1). It has been noted that the N-terminal sub-domain of the VWF type C module of a2 procollagen shows a structural similarity with the fibronectin FN1 module (O’Leary et al. 2004) and suggested that the VWF type ...
with the Cell Wall and Evidence that Peroxidase Does
with the Cell Wall and Evidence that Peroxidase Does

... Peroxidase (EC 1. 11. 1.7) is a heme containing glycopro- a concentration of 9 x 10-' M. The reaction is started by adding tein which utilizes H,02 to catalyze the oxidation of various 5 ,il of a peroxidase solution and measuring the increase in hydrogen donors. This enzyme is found both in the cyto ...
Muscle arm development in Caenorhabditis elegans
Muscle arm development in Caenorhabditis elegans

... neck muscles in each quadrant (Fig. 2A). As development continued, two more distal BWMs posterior to the initial four in each quadrant also expressed Mb::YFP, albeit at lower levels (Fig. 1D,E). We were able to assign unambiguous identities to the 16 BWMs that expressed Mb::YFP brightly from him-4p ...
Host cell interactions of outer membrane vesicle-associated
Host cell interactions of outer membrane vesicle-associated

Development, structure, and maintenance of C
Development, structure, and maintenance of C

... control by zygotically expressed genes (Baugh and Hunter, 2006). In the C and D lineages at least, pal-1 initially acts as a maternal factor for body wall muscle cell fate (Hunter and Kenyon, 1996; Edgar et al., 2001). pal-1 encodes a Caudal-related homeobox transcription factor (Hunter and Kenyon, ...
Auxinas
Auxinas

... Reprinted with permission from Macmillan Publishers, Ltd. Robert, H.S., and Friml, J. (2009) Auxin and other signals on the move in plants. Nat. Chem. Biol. 5: 325-332. Reprinted from Muday, G.K., and DeLong, A. (2001). Polar auxin transport: Controlling where and how much. Trends Plant Sci. 6: 535– ...
Factors Involved in Fruit Calcium Deficiency Disorders
Factors Involved in Fruit Calcium Deficiency Disorders

... avoid toxicity and cell death (White and Broadley 2003). For this reason, Ca2þ concentration inside living phloem cells is extremely low, which makes the phloem vessels incapable of contributing to growing leaves and fruit Ca2þ requirements (Taylor and Locascio 2004; Ho and White 2005). Indeed, it h ...
Cytoskeleton: What Does GTP Do for Septins? Dispatch
Cytoskeleton: What Does GTP Do for Septins? Dispatch

... morphology. Subsequent work suggested that septins are the building blocks of the neck filaments which organize the thin neck of cytoplasm separating the mother and bud. [1]. Septins are also present in metazoan cells, where they are required for cytokinesis in some systems, and implicated in a vari ...
endoglucanase in cellulose biosynthesis is not very clear
endoglucanase in cellulose biosynthesis is not very clear

... chains. Advantages of using a bacterial system for production of cellulose is that the bacterium grows rapidly under controlled conditions and produces cellulose from a variety of carbon sources including glucose, ethanol, sucrose, and glycerol. Moreover, genetic analysis is aided by the isolation o ...
Molecular and cellular requirements for the
Molecular and cellular requirements for the

SCARFACE Encodes an ARF-GAP That Is Required for Normal
SCARFACE Encodes an ARF-GAP That Is Required for Normal

... roles for PIN and MDR/PGP gene products in polar auxin transport in planta remain to be elucidated. Nevertheless, localization of auxin efflux–related proteins, such as PINs and PGPs, is clearly critical for directional auxin movement An important regulator of PIN protein localization is GN/ EMB30, ...
The dehydratase ADT3 affects ROS homeostasis
The dehydratase ADT3 affects ROS homeostasis

... cotyledons enter an aberrant developmental program that results in abnormal morphology ...
NCAM Polypeptides in Heart Development: Association with Z Discs
NCAM Polypeptides in Heart Development: Association with Z Discs

... that contains large amounts of polysialic acid (Cunningham ctal., 1987). Several variant forms of N C A M are produced by alternative m R N A splicing from a single gene. The best known of these variants are three forms of brain N C A M that differ in their attachment to the plasma membrane. One for ...
Artk96_CyclGMPReview_2016 - Pure
Artk96_CyclGMPReview_2016 - Pure

... coding affects Ca2+ binding proteins that show different affinities. For example, Ca2+ dependent kinases with different binding constants will be activated in response to different levels of cytoplasmic Ca2+. For the cyclic nucleotides 3’,5’ cAMP and 3’,5’ cGMP different signal amplitudes have been ...
Cytokinins regulate vascular morphogenesis in the Arabidopsis
Cytokinins regulate vascular morphogenesis in the Arabidopsis

... forms a continuous network of strands, called vascular bundles, which functionally connect the organs of a plant. In dicotyledonous species, vascular bundles are composed of several types of tissues: the meristematic tissues, procambium or vascular cambium, and the transporting tissues the xylem and ...
The Swimming Setae of Daphnia carinata By W. E. AGAR
The Swimming Setae of Daphnia carinata By W. E. AGAR

... segment T it divides into three branches which run into the bases of the terminal setae. A little lower down, about the middle of T, ganglion cells are inserted on its course, usually arranged in an upper group of three, and a lower one of two, cells. In the dorsal ramus only, a twig from the main n ...
PDF
PDF

... The establishment of cell type-specific dendritic arborization patterns is a key phase in the assembly of neuronal circuitry that facilitates the integration and processing of synaptic and sensory input. Although studies in Drosophila and vertebrate systems have identified a variety of factors that ...
PDF
PDF

... cytosolic material within the double membrane-bound autophagosome. The source of lipid contributing to this membrane growth has not been established. (C) Fusion of the autophagosomal outer membrane with lysosomes results in hydrolytic digestion of the inner membrane and the sequestered material, and ...
Chitin and Chitosan in Fungi - Wiley-VCH
Chitin and Chitosan in Fungi - Wiley-VCH

... readers who are not familiar with these aminoglucans should consult also Chapter 15, this volume (Chitin and Chitosan from Animal Sources). ...
Biofilms  are  described  as  surface ... et al.,
Biofilms are described as surface ... et al.,

... nutrients and accumulation of toxin byproducts generated by primary colonizers may limit the species diversity within a biofilm (Marsh, 1995). 2.2.2 The secondary stage The secondary stage involves the anchoring of bacteria to the surface by molecular mediated binding between specific adhesins and t ...
Inositol trisphosphate and calcium oscillations
Inositol trisphosphate and calcium oscillations

... The main feature of the luminal loading Ca2+ oscillation model is that the timing of each Ca2+ spike is determined by the periodic loading of the endoplasmic reticulum with Ca2+ (Figure 1). The best way of describing how this oscillator might function is to follow what happens when cells are stimula ...
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Endomembrane system

The endomembrane system is composed of the different membranes that are suspended in the cytoplasm within a eukaryotic cell. These membranes divide the cell into functional and structural compartments, or organelles. In eukaryotes the organelles of the endomembrane system include: the nuclear membrane, the endoplasmic reticulum, the Golgi apparatus, lysosomes, vesicles, endosomes and the cell membrane. The system is defined more accurately as the set of membranes that form a single functional and developmental unit, either being connected directly, or exchanging material through vesicle transport. Importantly, the endomembrane system does not include the membranes of mitochondria or chloroplasts.The nuclear membrane contains two lipid bilayers that encompass the contents of the nucleus. The endoplasmic reticulum (ER) is a synthesis and transport organelle that branches into the cytoplasm in plant and animal cells. The Golgi apparatus is a series of multiple compartments where molecules are packaged for delivery to other cell components or for secretion from the cell. Vacuoles, which are found in both plant and animal cells (though much bigger in plant cells), are responsible for maintaining the shape and structure of the cell as well as storing waste products. A vesicle is a relatively small, membrane-enclosed sac that stores or transports substances. The cell membrane, is a protective barrier that regulates what enters and leaves the cell. There is also an organelle known as the Spitzenkörper that is only found in fungi, and is connected with hyphal tip growth.In prokaryotes endomembranes are rare, although in many photosynthetic bacteria the plasma membrane is highly folded and most of the cell cytoplasm is filled with layers of light-gathering membrane. These light-gathering membranes may even form enclosed structures called chlorosomes in green sulfur bacteria.The organelles of the endomembrane system are related through direct contact or by the transfer of membrane segments as vesicles. Despite these relationships, the various membranes are not identical in structure and function. The thickness, molecular composition, and metabolic behavior of a membrane are not fixed, they may be modified several times during the membrane's life. One unifying characteristic the membranes share is a lipid bilayer, with proteins attached to either side or traversing them.
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