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REVIEWS Structural insights into the function of the
REVIEWS Structural insights into the function of the

Antibodies in plantscience
Antibodies in plantscience

Role of the leader sequence in tobacco pectin methylesterase
Role of the leader sequence in tobacco pectin methylesterase

... 0014-5793/$32.00  2006 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved. ...
Pontin and Reptin regulate cell proliferation in early Xenopus
Pontin and Reptin regulate cell proliferation in early Xenopus

... We then asked whether xPontin or xReptin are required for cell proliferation. For this purpose, antisense morpholino oligonucleotides against xPontin (PMo) and xReptin (RMo) were designed. Corresponding morpholino oligonucleotides with 4 mismatched bases were generated as control (CoMo). Antisense, ...
Polar auxin transport and patterning
Polar auxin transport and patterning

1. The BCL-2 Family Reunion.
1. The BCL-2 Family Reunion.

... (Figure 1A) (Riedl and Salvesen, 2007). Temporally, MOMP indeterminately occurs following proapoptotic stress, but studies suggest that this timing is dependent on the concentrations of diverse cellular proteins (Spencer et al., 2009). After MOMP, caspase activation and apoptosis ensue often within ...
Smooth Muscle Smooth Muscle Structure
Smooth Muscle Smooth Muscle Structure

... Sexual stimulation initiates the release of NO from nerve endings and cells inside the penis. Nitric Oxide then activates specific enzymes which result in increased levels of cGMP. Its actually cGMP that causes the smooth muscle around the penile arteries to relax, allowing these arteries to dilate. ...
Slow Changes in the Availability of Voltage
Slow Changes in the Availability of Voltage

Auxin and other signals on the move in plants
Auxin and other signals on the move in plants

... by one or several different transport mechanisms. Mineral nutrients, such as nitrogen, sulfur, phosphorus, magnesium, calcium and potassium, are taken up from the soil as inorganic materials, assimilated into organic compounds and transported through the plant depending on the balance between nutrie ...
Protein Cross-linkers handbook and selection guide
Protein Cross-linkers handbook and selection guide

... esters or the water soluble, membrane impermeable N-hydroxysulfosuccinimide (sulfo-NHS) esters. Addition of a charged sulfonate (SO3-) on the N-hydroxysuccinimide ring of the sulfo-NHS esters results in their solubility in water (~10mM), but not permeable to plasma membranes. The solubility and impe ...
physiology of ion transport across the tonoplast of higher plants
physiology of ion transport across the tonoplast of higher plants

... It is not surprising that the first plant vacuolar transport protein(s) to be cloned belong to the vacuolar type H+-ATPase (V-ATPase). These primary active transporters represent a ubiquitous class of proton pumps that are found on a variety of cellular organelles including lysosomes, endosomes, sec ...
A molecular framework for the inhibition of
A molecular framework for the inhibition of

... Ioio et al. 2007). In addition to auxin and cytokinin, other hormones have been recognized as modulating root development, such as gibberellins, ethylene and abscisic acid (ABA). It has recently been shown that gibberellins regulate Arabidopsis root growth by promoting cell proliferation (Achard et ...
- RichardWheeler.net
- RichardWheeler.net

Stimulation of Cell Elongation by Tetraploidy in Hypocotyls of Dark
Stimulation of Cell Elongation by Tetraploidy in Hypocotyls of Dark

... autopolyploidy and endopolyploidy, both of which have been correlated with cytoplasmic volume and, hence, cell growth [1]. Endopolyploidy, which is essential for angiosperm development, is controlled by an endoreduplication cycle whereby the genome is duplicated without cellular division. Conversely ...
Actin-dependent vacuolar occupancy of the cell determines auxin
Actin-dependent vacuolar occupancy of the cell determines auxin

... projections (Fig. 1 D–F), suggesting a higher degree of actin filament bundling (14) in entire cells. Our data suggest that auxin signaling also influences actin-dependent processes beyond the plasma membrane, leading to a tighter network of actin filaments. We recently reported that auxin controls ...
Charakterisierung peroxisomaler und Lipid
Charakterisierung peroxisomaler und Lipid

... Mitotracker Orange to stain the mitochondria. The bar represents 1 mm, unless indicated otherwise. L, lipid body; M, mitochondrion; N, nucleus; P, peroxisome; V, vacuole. Taken with modifications from (107). The peroxisomal matrix harbours at least 50 different enzymes that are linked to diverse bio ...
The safe and just space - University of Copenhagen
The safe and just space - University of Copenhagen

... absence of extracellular calcium. The ionophore renders membranes permeable to calcium, thereby releasing intracellular stores of calcium in the absence of a physiological stimulus. Treatment with the calcium ionophore mimicked the effect of IP3, thus implicating calcium as an intermediary in the IP ...
Proteomic Approach to Identify Novel
Proteomic Approach to Identify Novel

Analysis of the Juxtamembrane Dileucine Motif in the Insulin Receptor
Analysis of the Juxtamembrane Dileucine Motif in the Insulin Receptor

... To investigate the importance of the dileucine pair at positions 986 and 987 in the juxtamembrane domain of the insulin receptor, we mutated the LeuLeu residues to MetSer and AlaAla, and expressed the various recombinant receptors in NIH-3T3 cells. Metabolic labeling studies confirmed that the mutan ...
K transport in plants: Physiology and molecular biology Mark W. Szczerba
K transport in plants: Physiology and molecular biology Mark W. Szczerba

... Bloom, 2005; Gierth and Mäser, 2007; Lebaudy et al., 2007; Britto and Kronzucker, 2008). The high-affinity transport system (HATS) is a saturable system catalyzing the thermodynamically active uptake of K+ at low concentrations (o1 mM). The active influx of K+ is thought to be coupled to the passive ...
Mitochondrial Proton Leak and the Uncoupling Proteins
Mitochondrial Proton Leak and the Uncoupling Proteins

... amino acid identity to UCP1. An important feature of UCP2 was that it appeared to be expressed in nearly all cell types and could thus be a candidate for the mechanism of the ubiquitous basal proton conductance. This initial interpretation has since been revised somewhat. For example, UCP2 mRNA orig ...
A Difference between Rods and Cones in the Renewal of Outer
A Difference between Rods and Cones in the Renewal of Outer

... component of newly assembled membranous discs, which are then gradually displaced sclerally. In cones, however, no such concentration of radioactive protein has been observed. On the contrary, protein delivered to the outer segment becomes diffusely distributed throughout that structure. TJuis, in t ...
Bacterial chromosome segregation
Bacterial chromosome segregation

... defined time in the cell cycle and at the single, defined site on the chromosome, the replication origin oriC. The oriC region usually contains binding sites for the initiator protein DnaA (DnaA boxes) and repeats of an AT-rich sequence (13-mers) (reviewed by Thomas & Jagura-Burdzy, 1991). The chrom ...
Diatoms
Diatoms

... Most of the time, diatoms reproduce asexually by mitotic cell divisions. Each daughter cell receives half the cell wall of the original diatom and ...
PDF
PDF

... Mammalian lenses develop from a surface ectoderm-derived vesicle. The anterior cells of the vesicle differentiate into lens epithelial cells, while the cells that make up the posterior half of the vesicle differentiate into primary fiber cells. A single layer of epithelial cells lines the anterior h ...
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Endomembrane system

The endomembrane system is composed of the different membranes that are suspended in the cytoplasm within a eukaryotic cell. These membranes divide the cell into functional and structural compartments, or organelles. In eukaryotes the organelles of the endomembrane system include: the nuclear membrane, the endoplasmic reticulum, the Golgi apparatus, lysosomes, vesicles, endosomes and the cell membrane. The system is defined more accurately as the set of membranes that form a single functional and developmental unit, either being connected directly, or exchanging material through vesicle transport. Importantly, the endomembrane system does not include the membranes of mitochondria or chloroplasts.The nuclear membrane contains two lipid bilayers that encompass the contents of the nucleus. The endoplasmic reticulum (ER) is a synthesis and transport organelle that branches into the cytoplasm in plant and animal cells. The Golgi apparatus is a series of multiple compartments where molecules are packaged for delivery to other cell components or for secretion from the cell. Vacuoles, which are found in both plant and animal cells (though much bigger in plant cells), are responsible for maintaining the shape and structure of the cell as well as storing waste products. A vesicle is a relatively small, membrane-enclosed sac that stores or transports substances. The cell membrane, is a protective barrier that regulates what enters and leaves the cell. There is also an organelle known as the Spitzenkörper that is only found in fungi, and is connected with hyphal tip growth.In prokaryotes endomembranes are rare, although in many photosynthetic bacteria the plasma membrane is highly folded and most of the cell cytoplasm is filled with layers of light-gathering membrane. These light-gathering membranes may even form enclosed structures called chlorosomes in green sulfur bacteria.The organelles of the endomembrane system are related through direct contact or by the transfer of membrane segments as vesicles. Despite these relationships, the various membranes are not identical in structure and function. The thickness, molecular composition, and metabolic behavior of a membrane are not fixed, they may be modified several times during the membrane's life. One unifying characteristic the membranes share is a lipid bilayer, with proteins attached to either side or traversing them.
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