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Robust mechanisms of ventral furrow invagination require the
Robust mechanisms of ventral furrow invagination require the

... constricted (Costa et al 1993), resulting in their assuming a wedge-like form (basal expansion). This second change in cell shape has been proposed to constitute the final push that drives furrow internalization (Costa et al 1993, Leptin 1995). Additionally, it has been suggested by a number of auth ...
effect of ion concentration changes in t
effect of ion concentration changes in t

... was 10700 μm2 . To keep all geometric parameters of the model cell consistent with surface/volume ratios (Atot /Vcell = 0.729 μm2 /μm3 , Atub /Vcell = 0.403 μm2 /μm3 , Atub /Vcell = = 0.327 μm2 /μm3 ), fractional area of t-tubular membrane (0.56) and mean radius of the t-tubules (210 nm) published b ...
Expanded GAA repeats impair FXN gene expression and reposition
Expanded GAA repeats impair FXN gene expression and reposition

... Figure 1. The expanded GAA repeat FXN transgene associates with the NL more frequently in an FXN-GAA-MS2-Luc cell model. (A) Schematic representation of the pBACFXN-MS2-Luc and pBAC-FXN-GAA-MS2-Luc vectors. Each vector carries either six or ∼310 GAA repeats in intron 1, an array of 24 MBS in exon 2 ...
01 The Human Body: An Orientation
01 The Human Body: An Orientation

... *Please think about the fact that our bodies are made of atoms, the same thing as rocks, plants, stars and comets! By understanding the nature of specific atomic structures, you will become aware of how chemistry provides the basis of living systems. Chemistry is a simple matter of balancing electro ...
Acute renal dysfunction or failure is a common occurrence in the
Acute renal dysfunction or failure is a common occurrence in the

... is used to filter plasma water and solute across a membrane. This fluid is then discarded. The properties of the membrane determine the size and charge of the solute that can be removed and the rate at which water will be filtered for any given driving pressure. This process mimics the function of t ...
Here - New Mexico State University
Here - New Mexico State University

... Mamiko Yajima and Gary Wessel Brown University, Providence RI The process of germ line determination uses many conserved genes yet is highly variable in mechanism. Often closely related animals utilize one or another of the two basic mechanisms: Flies, worms, fish, and frogs use mechanisms based on ...
Inflammation and proliferation – a causal event of host response to
Inflammation and proliferation – a causal event of host response to

... pylori is a multifactorial process and is characterized by gastric epithelial cell injury and infiltration of the mucosa with both acute and chronic inflammatory cells (Smoot et al., 1999; Yang et al., 2012a). The virulence factors produced by H. pylori inflict direct damage on gastric epithelial ce ...
pdf: Rahman et al. 2007.
pdf: Rahman et al. 2007.

... (Stasinopoulos and Hangarter, 1990). To characterize the mechanism of inhibition of root elongation by auxin, we pursued a kinematic approach. First, we evaluated the time course of root elongation rate (Figure 1a–c). Seedlings were grown for 4 days, transplanted onto medium with auxins as indicated ...
Passive transfer of anti-herpes simplex virus type 2
Passive transfer of anti-herpes simplex virus type 2

... Purpose. To investigate whether passive transfer of antibodies to viral glycoproteins would protect against herpes simplex virus type 2-induced stromal keratitis. Methods. Balb/c mice were infected on the scarified cornea with herpes simplex virus types 1 or 2 (HSV-1 and HSV-2, respectively), and mo ...
PDF
PDF

... 2009; Poché et al., 2009), in which endothelial cell (EC) membranes and nuclei were labeled with mCherry and yellow fluorescent protein (YFP), respectively, similar to our previous studies (Udan et al., 2013). For embryos between E8.5 and E9.5, we used agarose cylinders with a 4.7 mm outer diameter ...
Farnesoid X Receptor Ligands Inhibit Vascular Smooth Muscle Cell
Farnesoid X Receptor Ligands Inhibit Vascular Smooth Muscle Cell

... PDGF-BB–stimulated conditions was abolished by 6ECDCA. In slight contrast, PDGF-BB–stimulated but not basal HASMC migration was inhibited by 6ECDCA. This difference most likely reflects that the RASMC cell line is already partially active in culture (as seen by low levels of basally expressed iNOS a ...
E-cadherin controls β-catenin and NF
E-cadherin controls β-catenin and NF

... transfected either to control or to LS174T cells transfected with ΔTCF4 or β-catenin siRNA in the absence of doxycycline. As shown in Fig. 2B, the increase in fibronectin RNA after Snail1 transfection was higher than the increase of LEF1, which probably reflected the lower expression of fibronectin ...
Mating behavior, male sensory cilia, and polycystins
Mating behavior, male sensory cilia, and polycystins

... cilia [4]. The cilia of the olfactory epithelium, in which odor transduction takes place, have a simple morphology, but each cell has a multitude of cilia [5]. Such differences in form and functions of cilia in different cells and tissues suggest that cilia are not all alike, despite the fact that c ...
Biochemical characterization of the vanilloid receptor 1 expressed in
Biochemical characterization of the vanilloid receptor 1 expressed in

... for VR1 overexpressed in CHO cells as well as in a dorsal root ganglia (DRG) crude membrane fraction. The glycosylation state of VR1 was found to depend on the expression system. Remarkably, endogenous VR1 in rat DRG was not found to be glycosylated [6] in contrast to what would be expected for an i ...
Cell death by autophagy: facts and apparent artefacts
Cell death by autophagy: facts and apparent artefacts

... cellular stress, as caused by starvation and increased metabolism (as in cancer cells), it is not surprising that cells undergoing death after stress also show features of autophagy. For example, cultured cells deprived of survival factors (such as serum) will undergo apoptosis, but as they go throu ...
letters - Cytomorpholab
letters - Cytomorpholab

... eight rounds of division, the syncytium contains approximately 200 nuclei that become partitioned into mononucleate cells by a cytokinetic mechanism that is referred to as cellularization12. Cellularization initiates from the anterior pole, where the embryo develops, and continues towards peripheral ...
Precipitation of calcium, magnesium, strontium and barium in tissues
Precipitation of calcium, magnesium, strontium and barium in tissues

... which is dominant in the vegetation of arid Australia [19], and plays an important role in nutrient, water and carbon cycling in arid ecosystems [20,21]. Many Australian Acacia species are adapted to a range of habitats and soil types [22] and are preferred for land restoration after mining [23]. Bi ...
Understanding Your Test Results - International Myeloma Foundation
Understanding Your Test Results - International Myeloma Foundation

... and SMM) are identified as the result of blood tests routinely ordered as part of an annual medical exam, such as the CBC. The CBC is a blood test that quantifies all the cells that make up the solid parts of blood. (The liquid part of blood, in which the blood cells are suspended, is colorless, and ...
Name: B2 6 Mark Questions Date: Time: Total marks available: Total
Name: B2 6 Mark Questions Date: Time: Total marks available: Total

... (ii) John washes his clothes using a biological washing powder on a 60 °C washing programme. Explain why the clothes were not washed as well as he had hoped. ...
Physiological study of larval fishes: challenges and opportunities
Physiological study of larval fishes: challenges and opportunities

... monitoring flow ejected from the anterior end of the conus arteriosus. Heart rate was derived from the primary blood pressure signal. Such measurements, innovative when Pelster and Bemis (1991) carried them out, are now routine. See text for additional details. ...
Here - Events
Here - Events

... To monitor cell growth over several cell cycles we developed two volume measurement methods. The first method is based on fluorescence-exclusion and enables precise growth rate measurement throughout the cell cycle. The second method uses confinement inside micro-channels and enables inducing asymme ...
Lessons on longevity from budding yeast
Lessons on longevity from budding yeast

... model organisms used in ageing-related research. In comparison with other systems, the relative ease and rapidity with which longevity can be quantified in yeast has allowed rapid progress is defining the molecular mechanisms of ageing in this organism and the identification of dozens of factors tha ...
Immunohistochemical sweat gland profiles
Immunohistochemical sweat gland profiles

... physiologic conditions, CD138 expression is restricted to the epidermis, the outer root sheath of the anagen hair follicle, and the sweat gland epithelium. The present study confirms the presence of CD138 in sweat glands. In addition, we stress the fact that the luminal part of the acrosyringium is ...
Stem Cell Therapy In Pediatric Neurological Disorders
Stem Cell Therapy In Pediatric Neurological Disorders

... for the science of the future to change, if possible, this harsh decree." However, recent progress in regenerative medicine has provided hope that injured CNS can be repaired using stem cells. Stem cells are unspecialized cells with a capacity of self renewal and differentiation into specialized cel ...
SYBR Green Cells-to-CT™ Control Kit
SYBR Green Cells-to-CT™ Control Kit

... Controls for the Power SYBR Green and Fast SYBR Green Cells-to-CT Kits The SYBR Green Cells-to-CT Control Kit is designed for use with SYBR Green-based Cells-to-CT Kits. Refer to the protocol for the appropriate kit for detailed instructions, available under the "Literature/Resources" tab at its web ...
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Organ-on-a-chip

An organ-on-a-chip (OC) is a multi-channel 3-D microfluidic cell culture chip that simulates the activities, mechanics and physiological response of entire organs and organ systems. It constitutes the subject matter of significant biomedical engineering research, more precisely in bio-MEMS. The convergence of labs-on-chips (LOCs) and cell biology has permitted the study of human physiology in an organ-specific context, introducing a novel model of in vitro multicellular human organisms. One day, they will perhaps abolish the need for animals in drug development and toxin testing.Although multiple publications claim to have translated organ functions onto this interface, the movement towards this microfluidic application is still in its infancy. Organs-on-chips will vary in design and approach between different researchers. As such, validation and optimization of these systems will likely be a long process. Organs that have been simulated by microfluidic devices include the heart, the lung, kidney, artery, bone, cartilage, skin and more.Nevertheless, building valid artificial organs requires not only a precise cellular manipulation, but a detailed understanding of the human body’s fundamental intricate response to any event. A common concern with organs-on-chips lies in the isolation of organs during testing. ""If you don’t use as close to the total physiological system that you can, you’re likely to run into troubles"" says William Haseltine, founder of Rockville, Maryland. Microfabrication, microelectronics and microfluidics offer the prospect of modeling sophisticated in vitro physiological responses under accurately simulated conditions.
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