CHAPTER 3: Early tissue patterning is recreated by MEFs in... Figure 5: Osteogenic and chondrogenic differentiation of MEFs cultured in
... Moreover, a dose-response inhibitory effect on the 3D-bilateral structure development can be observed when diluted concentrations of Staurosporine are added to the cultures (Figure 2 F). These results indicate that during this morphogenetic event the cell density increases not only by contraction bu ...
... Moreover, a dose-response inhibitory effect on the 3D-bilateral structure development can be observed when diluted concentrations of Staurosporine are added to the cultures (Figure 2 F). These results indicate that during this morphogenetic event the cell density increases not only by contraction bu ...
Shared versus Specialized Glycinergic Spinal Interneurons in Axial
... (⫺12.2 ⫾ 2.7 mV), and fired repetitively in response to depolarizing current injections. Of all 23 cells tested, 8 showed evidence of spike frequency adaptation. The first description of CoLA morphology was based on a retrograde labeling technique and revealed an ascending axon of five segments or l ...
... (⫺12.2 ⫾ 2.7 mV), and fired repetitively in response to depolarizing current injections. Of all 23 cells tested, 8 showed evidence of spike frequency adaptation. The first description of CoLA morphology was based on a retrograde labeling technique and revealed an ascending axon of five segments or l ...
Motilities Swimming, Swarming, and Twitching Adherent Phenotypic
... Pseudomonas aeruginosa is a ubiquitous environmental bacterium capable of forming biofilms on surfaces as a survival strategy. It exhibits a large variety of competition/virulence factors, such as three types of motilities: flagellum-mediated swimming, flagellum-mediated swarming, and type IV pilus- ...
... Pseudomonas aeruginosa is a ubiquitous environmental bacterium capable of forming biofilms on surfaces as a survival strategy. It exhibits a large variety of competition/virulence factors, such as three types of motilities: flagellum-mediated swimming, flagellum-mediated swarming, and type IV pilus- ...
The FluidMosaic Model of the Structure of Cell Membranes
... the major portion of the phospholipids lipid molecules on the average. (ii) None is in bilayer form in a variety of intact of the experiments mentioned above is membranes. For example, differential sufficiently sensitive and quantitative to calorimetry of intact mycoplasma mem- prove whether 100 per ...
... the major portion of the phospholipids lipid molecules on the average. (ii) None is in bilayer form in a variety of intact of the experiments mentioned above is membranes. For example, differential sufficiently sensitive and quantitative to calorimetry of intact mycoplasma mem- prove whether 100 per ...
High levels of tRNA abundance and alteration of tRNA charging by
... 1.2- to 1.7-fold. These results demonstrate that tRNA levels are elevated in MM cells which accommodate for their aberrantly high translation activities. Mitochondrial translation may be less affected in MM as seen by the smaller increase in abundance of mitochondrial tRNA. The relative abundance of ...
... 1.2- to 1.7-fold. These results demonstrate that tRNA levels are elevated in MM cells which accommodate for their aberrantly high translation activities. Mitochondrial translation may be less affected in MM as seen by the smaller increase in abundance of mitochondrial tRNA. The relative abundance of ...
Characterization and Biological Activities of Ocellatin Peptides from
... sequencing and by cDNA cloning. Structural similarities between them and other antimicrobial peptides from the skin secretion of Leptodactylus genus frogs were found. Ocellatins-PT1 to -PT5 (25 amino acid residues) are amidated at the C-terminus, while ocellatins-PT6 to -PT8 (32 amino acid residues) ...
... sequencing and by cDNA cloning. Structural similarities between them and other antimicrobial peptides from the skin secretion of Leptodactylus genus frogs were found. Ocellatins-PT1 to -PT5 (25 amino acid residues) are amidated at the C-terminus, while ocellatins-PT6 to -PT8 (32 amino acid residues) ...
"Monoclonal Antibodies to Human Cell Surface Antigens". In
... of leukocyte surface molecules. The antibodies have been even more useful as markers for cell populations, allowing the counting, separation, and functional study of numerous subsets of cells of the immune system. A series of international workshops were instrumental in coordinating this development ...
... of leukocyte surface molecules. The antibodies have been even more useful as markers for cell populations, allowing the counting, separation, and functional study of numerous subsets of cells of the immune system. A series of international workshops were instrumental in coordinating this development ...
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... with the standard mixture of TRDA and Fast Green. Embryos were then fixed and silver stained at the endpoints described above. After fixation, these experimental embryos were screened to ensure that they contained no cells labelled with FDA, the lineage tracer used for the ablations. Preparation of ...
... with the standard mixture of TRDA and Fast Green. Embryos were then fixed and silver stained at the endpoints described above. After fixation, these experimental embryos were screened to ensure that they contained no cells labelled with FDA, the lineage tracer used for the ablations. Preparation of ...
Out of the Mouths of Plants: The Molecular Basis of the Evolution
... upward and outward water movement in plants and cools the plant surface via thermal dissipation. The distribution of stomata is far from random; they are evenly distributed, an adaptation allowing gases to reach interior tissues effectively, and they adhere closely to the one-cell spacing rule (Sach ...
... upward and outward water movement in plants and cools the plant surface via thermal dissipation. The distribution of stomata is far from random; they are evenly distributed, an adaptation allowing gases to reach interior tissues effectively, and they adhere closely to the one-cell spacing rule (Sach ...
Acute kidney injury (AKI)
... – eGFR <30 – Hospital admission, Nephrology consult, Dialysis planning, renal protection – eGFR 30-59 – Discontinue NSAIDs, IV volume expansion, Intra-arterial: isoosmolar, Intravenous: iso-osmolar or low osmolar contrast; limit contrast volume – eGFR >60, Discontinue metformin ...
... – eGFR <30 – Hospital admission, Nephrology consult, Dialysis planning, renal protection – eGFR 30-59 – Discontinue NSAIDs, IV volume expansion, Intra-arterial: isoosmolar, Intravenous: iso-osmolar or low osmolar contrast; limit contrast volume – eGFR >60, Discontinue metformin ...
Methyl Jasmonate-Induced Ethylene Production
... evaporation of the volatile turpentine fractions (Katoh and Croteau, 1997; Phillips and Croteau, 1999). While there is considerable information about the anatomical and biochemical defenses in conifer stems, there is little known about the regulation of constitutive and inducible defenses. Recent wo ...
... evaporation of the volatile turpentine fractions (Katoh and Croteau, 1997; Phillips and Croteau, 1999). While there is considerable information about the anatomical and biochemical defenses in conifer stems, there is little known about the regulation of constitutive and inducible defenses. Recent wo ...
The Arabidopsis Rab GTPase family: another
... The plant endomembrane system comprises several biochemically distinct membrane-bound organelles that are linked by membrane traffic. More than 17% of all Arabidopsis gene products are predicted to enter the endomembrane system [1] and each must be transported to its correct destination. That each c ...
... The plant endomembrane system comprises several biochemically distinct membrane-bound organelles that are linked by membrane traffic. More than 17% of all Arabidopsis gene products are predicted to enter the endomembrane system [1] and each must be transported to its correct destination. That each c ...
Nucleolus: the fascinating nuclear body
... nuclear functional compartmentalization (Strouboulis and WolVe 1996). It is the site of ribosome biogenesis and in addition the nucleolar machineries are distributed in diVerent compartments. When observed by EM, three main nucleolar components (compartments) can be discerned in mammalian cells: the ...
... nuclear functional compartmentalization (Strouboulis and WolVe 1996). It is the site of ribosome biogenesis and in addition the nucleolar machineries are distributed in diVerent compartments. When observed by EM, three main nucleolar components (compartments) can be discerned in mammalian cells: the ...
Cell adhesion and cell mechanics during zebrafish
... to a vast number of cells which subsequently have to share specialized tasks and build complex structures such as eyes, heart or brain (see Fig. 1.1). But how can cells that originate from a single ancestor control all these processes? Surely, an important aspect are differences in gene expression a ...
... to a vast number of cells which subsequently have to share specialized tasks and build complex structures such as eyes, heart or brain (see Fig. 1.1). But how can cells that originate from a single ancestor control all these processes? Surely, an important aspect are differences in gene expression a ...
Xenopus ADAM13 and ADAM19 are Important for Proper
... Ph.D., UNIVERSITY OF MASSACHCUSETTS AMHERST Directed by: Professor Dominique Alfandari ...
... Ph.D., UNIVERSITY OF MASSACHCUSETTS AMHERST Directed by: Professor Dominique Alfandari ...
as a PDF
... Because the anti– a-TIP and anti– d-TIP antibodies frequently were observed to label the same organelles (see below), we performed an additional control experiment to demonstrate their specificity, as shown in Figure 1C. Protein gel blot strips of pea root tip extracts were incubated with the anti–a ...
... Because the anti– a-TIP and anti– d-TIP antibodies frequently were observed to label the same organelles (see below), we performed an additional control experiment to demonstrate their specificity, as shown in Figure 1C. Protein gel blot strips of pea root tip extracts were incubated with the anti–a ...
The Assimilation of Amino-acids by Bacteria
... and entrance into the ‘metabolic pool’ of the cell, without protein formation (Gale & Mitchell, 1947). The protein synthesis takes place only in growing cells and can be eliminated by working with well-washed suspensions of cells. The remaining metabolism (transfer of glutamic acid to the metabolic ...
... and entrance into the ‘metabolic pool’ of the cell, without protein formation (Gale & Mitchell, 1947). The protein synthesis takes place only in growing cells and can be eliminated by working with well-washed suspensions of cells. The remaining metabolism (transfer of glutamic acid to the metabolic ...
First description of giant Archaea (Thaumarchaeota)
... This bacteria-free phenotype occurs much less frequently than the bacteria associated one (F. Muller and O. Gros, personal observations). The cell walls of both archaeal cell genotypes are considerably different from bacterial cell walls described for Gram-negative or Gram-positive marine bacteria ( ...
... This bacteria-free phenotype occurs much less frequently than the bacteria associated one (F. Muller and O. Gros, personal observations). The cell walls of both archaeal cell genotypes are considerably different from bacterial cell walls described for Gram-negative or Gram-positive marine bacteria ( ...
Tonoplast Intrinsic Protein Isoforms as Markers for Vacuolar Functions
... Because the anti– a-TIP and anti– d-TIP antibodies frequently were observed to label the same organelles (see below), we performed an additional control experiment to demonstrate their specificity, as shown in Figure 1C. Protein gel blot strips of pea root tip extracts were incubated with the anti–a ...
... Because the anti– a-TIP and anti– d-TIP antibodies frequently were observed to label the same organelles (see below), we performed an additional control experiment to demonstrate their specificity, as shown in Figure 1C. Protein gel blot strips of pea root tip extracts were incubated with the anti–a ...
Production of Outer Membrane Vesicles in a Clinical
... tryptone soy agar (TSA) and incubated at 37°C for 24h. An isolated colony was used to obtain OMVs by inoculation into 5 ml of tryptone soy broth (TSB) and incubation at 37°C overnight. One hundred plates with Craig agar (30 g/l of CAS-amino acids, 4 g/l yeast extract, 15 g/l bacteriological agar, 0. ...
... tryptone soy agar (TSA) and incubated at 37°C for 24h. An isolated colony was used to obtain OMVs by inoculation into 5 ml of tryptone soy broth (TSB) and incubation at 37°C overnight. One hundred plates with Craig agar (30 g/l of CAS-amino acids, 4 g/l yeast extract, 15 g/l bacteriological agar, 0. ...
dravhandling-hafte - DUO
... kidney cortex and provides an excellent model system to study epithelial cell polarity. In fact, it is the most commonly studied epithelial cell line which, when grown on permeable supports, forms well polarized monolayers that can be accessed from both the apical and basolateral surfaces (Zegers an ...
... kidney cortex and provides an excellent model system to study epithelial cell polarity. In fact, it is the most commonly studied epithelial cell line which, when grown on permeable supports, forms well polarized monolayers that can be accessed from both the apical and basolateral surfaces (Zegers an ...
Canadian Journal of Microbiology 47
... but has not yet been confirmed for P. myrsinacearum. However, Azospirillum is known to produce small quantities of acids, like indole-3-acetic acid and others (Baca et al. 1994) that might dissolve the thin alginate layers (0.3 µm or less) initially separating the microalgal and the bacterial coloni ...
... but has not yet been confirmed for P. myrsinacearum. However, Azospirillum is known to produce small quantities of acids, like indole-3-acetic acid and others (Baca et al. 1994) that might dissolve the thin alginate layers (0.3 µm or less) initially separating the microalgal and the bacterial coloni ...
PDF
... established transgenic lines using Gal4-VP16 cannot be regulated by these new methods (see Faucherre and Lopez-Schier, 2011). As an example, we show temporal control over GFP expression, mediated by regulating the activator Gal4, in early developing primary motoneurons (PMNs), but not later developi ...
... established transgenic lines using Gal4-VP16 cannot be regulated by these new methods (see Faucherre and Lopez-Schier, 2011). As an example, we show temporal control over GFP expression, mediated by regulating the activator Gal4, in early developing primary motoneurons (PMNs), but not later developi ...
Epigenetic and transcriptional regulation of early mouse development
... Figure 12: Transcriptome analysis of deregulated genes at gastrulation ....................................... 31 Figure 13: Ratio of expected and observed genotypes of ESCs........................................................ 32 Figure 14: Pluripotency markers are expressed in mutant ESCs ...... ...
... Figure 12: Transcriptome analysis of deregulated genes at gastrulation ....................................... 31 Figure 13: Ratio of expected and observed genotypes of ESCs........................................................ 32 Figure 14: Pluripotency markers are expressed in mutant ESCs ...... ...
Mitochondrial dysfunction and Parkinson`s disease genes: insights
... studies from rare genetic mutations give significant insights into addressing the pathological mechanism of not only familial PD, but also sporadic PD. Recent PD research focuses on generating genetic mutant animal models that recapitulate the features of human PD patients. Significant advances in P ...
... studies from rare genetic mutations give significant insights into addressing the pathological mechanism of not only familial PD, but also sporadic PD. Recent PD research focuses on generating genetic mutant animal models that recapitulate the features of human PD patients. Significant advances in P ...