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Reversible translocation of cytidylyltransferase between cytosol and
Reversible translocation of cytidylyltransferase between cytosol and

... 12-myristate 13-acetate, indicating that hydrolysis can be a rapid process [21]. Since the existence of a phosphatidylcholine cycle was recently proposed [22], it seemed conceivable that, for an efficient regulation of cell metabolism, resynthesis should be regulated in the same range of time as hyd ...
Receptor-mediated sorting of soluble vacuolar proteins: myths, facts
Receptor-mediated sorting of soluble vacuolar proteins: myths, facts

... Receptor–ligand complexes are then collected into CCVs, a process involving recognition of sorting signals in the cytosolic domain of the MPR firstly by monomeric adaptors (Golgi-localized, Gamma-ear-containing, ARF-binding proteins [GGAs]) then by tetrameric AP-1 adaptors (Bonifacino, 2004). After ...
O-GlcNAc transferase inhibitors: current tools and
O-GlcNAc transferase inhibitors: current tools and

... recently developed combining elements of both substrates. However, although these large molecules bind OGT with micromolar affinity, they are not cell-permeable and therefore as yet unsuitable for in vivo studies. All the compounds discussed in this review have been employed for several applications ...
ADRENERGIC ANTAGONIST PROPRANOLOL AS A NOVEL, EFFECTIVE SPERMICIDE:  AN NMR  STUDY Research Article 
ADRENERGIC ANTAGONIST PROPRANOLOL AS A NOVEL, EFFECTIVE SPERMICIDE:  AN NMR  STUDY Research Article 

... Although it was  at  one time  widely promoted  as a protective against  sexually transmitted infections including HIV, subsequent studies have  shown that it can in fact increase the risk of infection by damaging the  physical  barriers  of  the  rectum  or  vagina,  especially  if  used  frequentl ...
Part 3 (Archaea - Updates Book)
Part 3 (Archaea - Updates Book)

... b) Crenarcheota – some of these species live at the highest temperatures of any known living things—including in water hot enough to boil! (A variety have recently been discovered growing in soil and water at more moderate temperatures, however). c) Korarcheota are only known from their DNA sequence ...
Paramecium as a bioassay system for elucidation of cytotoxicity and
Paramecium as a bioassay system for elucidation of cytotoxicity and

... hazardous chemical substances by interaction between the metabolic products of culture medium and CNF. At this moment we have no information about chemical compositions of the culture medium. However, one candidate of the hazardous chemical substances is suggested by the following observation. One o ...
Salinity Effects on the Activity of Plasma Membrane H+ and Ca2+
Salinity Effects on the Activity of Plasma Membrane H+ and Ca2+

... treatment resulted in suppression of H ‡ pumping and caused a signi®cant shift towards net H ‡ in¯ux (Fig. 1C). What is even more important, vanadate treatment also prevented NaCl-induced H ‡ extrusion (compare Fig. 1A and C). These results suggest that the ATP-dependent H ‡ -pump (inhibited by vana ...
Electrical Properties of the Pacemaker Neurons in the Heart
Electrical Properties of the Pacemaker Neurons in the Heart

... The variation can probably be explained by the variation of cell size (from 30 to 80 ju in length). A small rectification was always observed in the I-V relation of a cell (Fig. 3). Electrotonic coupling was not observed between cells in the caudal region. In cells in the rostral region, however, co ...
Mochii - Salamander Genome Project
Mochii - Salamander Genome Project

... urodele limb regeneration. Notochord precursor cells accumulate to create a compact cell mass adjacent to the edge of the amputated notochord sheath (Fig. 1B). The cells proliferate and align along the proximal-to-distal direction to make an immature notochord (Fig. 1C), which continues to elongate ...
leishmania spp.: completely defined medium without serum and
leishmania spp.: completely defined medium without serum and

... Abstract. The elimination of serum or of serum-derived macromolecules that supplant the fetal calf serum requirement from Leishmania culture media could decrease costs and improve the feasibility of large-scale production of well-defined parasite material. We report a completely defined medium, with ...
ANALYSIS OF NF-κB, CASPASE-3, MMP-2, MMP
ANALYSIS OF NF-κB, CASPASE-3, MMP-2, MMP

... Classical Hodgkin lymphoma shows bimodal age distribution, with two peaks in young adult (15-35 years) and older age group in industrialized countries, and in childhood and older age groups in developing countries [7]. MCCHL is relatively more frequent in children and older adults and more common in ...
midlife crisis encodes a conserved zinc
midlife crisis encodes a conserved zinc

... A defining characteristic of stem cells is the ability to produce daughters that retain stem cell fate (self-renew) as well as daughters that begin the process of differentiation. The robust adoption of these distinct fates is crucial both for the maintenance of stem cell pool size and for the produ ...
Depletion of intracellular Ca2+ stores enhances flow
Depletion of intracellular Ca2+ stores enhances flow

... (Figure 1a). The diameter of vessels after stopping the flow was 65.671.5% (n ¼ 14) of the initial diameter (before phenylephrine preconstriction), and was comparable to that before the initiation of flow (66.771.3% of the initial diameter, British Journal of Pharmacology vol 147 (5) ...
Archaea Topics in Biodiversity
Archaea Topics in Biodiversity

... Stereochemistry of the glycerol group is the reverse of that found in other organisms. This implies that archaea use wholely different enzymes for synthesizing phospholipids than bacteria and eukaryotes. Such enzymes developed in very ancient geological times, suggesting an early split from the othe ...
measuring force in the developing zebrafish embryo using
measuring force in the developing zebrafish embryo using

... In addition to large-scale movement of tissue in sheets, changes in force are also capable of driving migration of cells acting as independent entities. This is particularly evident in a phenomenon called differential adhesion. According to the differential adhesion hypothesis, cells segregate base ...
The Cytoskeleton as a Regulator and Target of
The Cytoskeleton as a Regulator and Target of

... attain aggressive traits or lose beneficial ones. Plants must thus constantly refine existing defenses and develop new strategies to maintain an upper hand in their interactions with other organisms. Changes in the organization of the plant cytoskeleton during plant interactions with microbial and o ...
respiratory system
respiratory system

... is the space in which the gas exchange is not taking place. Some of the air that a person breathes never reaches the gas exchange areas but instead goes to fill the respiratory passages. The respiratory passages where no gas exchange takes place is called the anatomical dead space (which consist of ...
Apago PDF Enhancer
Apago PDF Enhancer

... There are three genera and about 65 living species of gnetophytes (phylum Gnetophyta). They are the only gymnosperms with vessels in their xylem. Vessels are a particularly efficient conducting cell type that is a common feature in angiosperms. The members of the three genera differ greatly from one ...
IL-10–producing NKT10 cells are a distinct regulatory invariant
IL-10–producing NKT10 cells are a distinct regulatory invariant

- Wiley Online Library
- Wiley Online Library

... any change in level between all three samples (Figure 2 and Table S3). These results suggest that SCs and GCs have almost identical histone expression patterns, which are significantly different from those of VCs. Identification of histone variants and isoforms To reveal histone variants expressed i ...
Neuronal polarity: an evolutionary perspective
Neuronal polarity: an evolutionary perspective

... facilitates directional information flow in complex vertebrate nervous systems. The topic we address here is when the key aspects of neuronal polarity evolved. All neurons have a central cell body with thin processes that extend from it to cover long distances, and they also all rely on voltage-gate ...
solvent selection for whole cell biotransformations in organic media
solvent selection for whole cell biotransformations in organic media

... for this purpose in microbiology. The effectiveness of the permeabilisation of E. coli  cells by toluene has been studied by Deutscher (1974), who found the time of  exposure, temperature, pH and growth stage all to be of importance. Using E. coli it  was demonstrated that the amount of protein rele ...
The FluidMosaic Model of the Structure of Cell Membranes
The FluidMosaic Model of the Structure of Cell Membranes

... the major portion of the phospholipids lipid molecules on the average. (ii) None is in bilayer form in a variety of intact of the experiments mentioned above is membranes. For example, differential sufficiently sensitive and quantitative to calorimetry of intact mycoplasma mem- prove whether 100 per ...
Motilities Swimming, Swarming, and Twitching Adherent Phenotypic
Motilities Swimming, Swarming, and Twitching Adherent Phenotypic

... Pseudomonas aeruginosa is a ubiquitous environmental bacterium capable of forming biofilms on surfaces as a survival strategy. It exhibits a large variety of competition/virulence factors, such as three types of motilities: flagellum-mediated swimming, flagellum-mediated swarming, and type IV pilus- ...
High levels of tRNA abundance and alteration of tRNA charging by
High levels of tRNA abundance and alteration of tRNA charging by

... 1.2- to 1.7-fold. These results demonstrate that tRNA levels are elevated in MM cells which accommodate for their aberrantly high translation activities. Mitochondrial translation may be less affected in MM as seen by the smaller increase in abundance of mitochondrial tRNA. The relative abundance of ...
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Organ-on-a-chip

An organ-on-a-chip (OC) is a multi-channel 3-D microfluidic cell culture chip that simulates the activities, mechanics and physiological response of entire organs and organ systems. It constitutes the subject matter of significant biomedical engineering research, more precisely in bio-MEMS. The convergence of labs-on-chips (LOCs) and cell biology has permitted the study of human physiology in an organ-specific context, introducing a novel model of in vitro multicellular human organisms. One day, they will perhaps abolish the need for animals in drug development and toxin testing.Although multiple publications claim to have translated organ functions onto this interface, the movement towards this microfluidic application is still in its infancy. Organs-on-chips will vary in design and approach between different researchers. As such, validation and optimization of these systems will likely be a long process. Organs that have been simulated by microfluidic devices include the heart, the lung, kidney, artery, bone, cartilage, skin and more.Nevertheless, building valid artificial organs requires not only a precise cellular manipulation, but a detailed understanding of the human body’s fundamental intricate response to any event. A common concern with organs-on-chips lies in the isolation of organs during testing. ""If you don’t use as close to the total physiological system that you can, you’re likely to run into troubles"" says William Haseltine, founder of Rockville, Maryland. Microfabrication, microelectronics and microfluidics offer the prospect of modeling sophisticated in vitro physiological responses under accurately simulated conditions.
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