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Data/hora: 03/04/2017 17:22:49 Provedor de dados: 189 País
Data/hora: 03/04/2017 17:22:49 Provedor de dados: 189 País

... the organelles and the cytosol. The Arabidopsis thaliana protein AtFOLT1 encoded by the At5g66380 gene is the closest homolog of the mitochondrial folate transporters (MFTs) characterized in mammalian cells. AtFOLT1 belongs to the mitochondrial carrier family, but GFP-tagging experiments and Western ...
Early transcription in Caenorhabditis elegans embryos
Early transcription in Caenorhabditis elegans embryos

... The time of onset for embryonic transcription in C. elegans has previously been assessed indirectly by two different methods, with somewhat differing results. In situ hybridization with labelled oligo(dT) probes to detect poly(A)+ mRNAs showed the first detectable increase in nuclear labelling at ap ...
A Geminivirus lnduces Expression of a Host DNA Synthesis Protein
A Geminivirus lnduces Expression of a Host DNA Synthesis Protein

... basis of geminivirus replication, we asked whether the TGMV replication proteins AL1 and AL3 are located in a subcellular compartment where plant DNA replication occurs, that is, the nucleus, the plastid, and/or the mitochondrion. In Figure 2, antibodies specific to AL1 or AL3 and secondary antibodi ...
Changes in Cell Morphology and the Cellular Localization of Protein
Changes in Cell Morphology and the Cellular Localization of Protein

... could suppress the dis1 phenotype. They named this novel protein dis1-suppressing protein kinase, or dsk1. Upon further characterization, it has been found that Dsk1 phosphorylates serine/arginine residues, and can autophosphorylate both tyrosine and serine-arginine residues. Phosphorylation states ...
PDF
PDF

... 150 units/mg) in 10 millilitres of medium, and hours or more until they were completely isolated bone cells were washed twice and by the trypan blue exclusion test (usually The cell concentration was determined by and phase contrast microscopy (normally ...
ADME 2
ADME 2

... at the acid pH of the stomach? 5. What can the intestinal microflora do to chemicals? 6. The rate at which foodstuffs pass through the GI tract? GIT motility has a significant effect on GIT absorption of a toxicant. For example, excessively rapid movement of gut contents can reduce absorption by red ...
The MAP Kinase MPK4 Is Required for Cytokinesis
The MAP Kinase MPK4 Is Required for Cytokinesis

... kinase kinase kinase and MAP kinase kinase (MAPKK) plays a key role in the control of plant cytokinesis. We show here that a MAP kinase, MPK4, is required for the formation of the cell plate in Arabidopsis thaliana. Single mutations in MPK4 caused dwarfism and characteristic defects in cytokinesis, ...
Liposome
Liposome

... prepared by extrusion is a function of the number of passes through the polycarbonate membrane of the hydrated lipid suspension. A minimum of eleven passes through the membrane is Liposome size as function of passes through recommended for most lipids to obtain an extruder polycarbonate membrane uni ...
Roles for Rice Membrane Dynamics and Plasmodesmata during
Roles for Rice Membrane Dynamics and Plasmodesmata during

... tissue is optically clear and relatively flat, which facilitates live-cell imaging (Koga et al., 2004). To assess whether major R gene– mediated specificity is maintained in leaf sheaths under our assay conditions, we compared compatible and incompatible interactions mediated by the Pi-ta resistance ...
Guidelines for Building Self-Organizing Maps
Guidelines for Building Self-Organizing Maps

... • Place observation in the cell with the most similar  weight (based on input variables)  (BMU) • Update neighboring cells to weights similar to the  situated cell • Place another observation • Adjust the weights Etc. • Repeat this process many times ...
JEFFERSON COLLEGE CONCEPTS IN BIOLOGY
JEFFERSON COLLEGE CONCEPTS IN BIOLOGY

... CATALOGUE DESCRIPTION Prerequisite: None 3 semester hours credit. Concepts in Biology is a non-laboratory course covering concepts common to all life forms. It focuses on structural and functional human systems. Concepts in Biology will partially fulfill the natural science requirement for the Assoc ...
A balanced pyrimidine pool is required for optimal Chk1 activation to
A balanced pyrimidine pool is required for optimal Chk1 activation to

... reflect a defect in sister chromatid segregation during anaphase. They cannot be stained with conventional DNA dyes or antibodies against histones, but they can be detected with an antibody against Plk1-interacting checkpoint helicase (PICH, also known as ERCC6L), a protein recruited to UFBs (Bauman ...
PDF
PDF

... along with various other cells, five bilateral pairs of stem cells or teloblasts: one mesodermal pair (M) and four ectodermal pairs (N, OiP, O/P, and Q; Fig. 1). Each teloblast produces a chain or bandlet of several dozen much smaller segmental founder cells called primary blast cells. Blast cells a ...
8879.full - The Journal of Neuroscience
8879.full - The Journal of Neuroscience

... blockade of this miRNA by antisense oligonucleotides only resulted in subtle phenotypes, with mild delays in differentiation and no detectable malformation (Cao et al., 2007; Visvanathan et al., 2007; Cheng et al., 2009). Based on these findings, it was concluded, that miR-124 regulates proliferatio ...
Slide PDF - The future of science
Slide PDF - The future of science

... Tissue/ ...
Human Body Systems Graphic Organizer
Human Body Systems Graphic Organizer

... exhale more forcefully - the maximum amount of air moved in and out of the lungs is called the VITAL CAPACITY. In this activity, you will be measuring the vital capacity and the tidal volume of your own lungs, this actual number can then be compared with a number derived from an equation that measur ...
Tasquinimod Modulates Suppressive Myeloid Cells and Enhances
Tasquinimod Modulates Suppressive Myeloid Cells and Enhances

... of SurVaxM peptide and 100 ng of GM-CSF by s.c. injection, once per week. The tumor size was measured by a caliper twice a week. At the end of the 3- to 4-week experiment, tumors and spleens were collected and analyzed. B16-h5T4 cells were cultured as described above, counted, resuspended, and maint ...
Functional differences between kindlin-1 and kindlin
Functional differences between kindlin-1 and kindlin

... the dermis, and the basement membrane (BM) was marked with laminin-5 (Fig. 3A,A9). In the conditional KO skin sections, b1 was not expressed in the epidermis (asterisk in Fig. 3B9), whereas the dermal expression was still strong and the BM quite disrupted, as judged by laminin-5 staining (Fig. 3B). ...
Examples of Ciliophorans
Examples of Ciliophorans

... Some live in the digestive tracts of hoofed mammals to counterbalance the large populations of bacteria that break down cellulose from the animal’s food. Some ciliates can cause diseases in fish. ...
4-4-05rev
4-4-05rev

... (in the renal cortex) to urea. – As the collecting duct traverses the gradient of osmolarity in the kidney, the filtrate becomes increasingly concentrated as it loses more and more water by osmosis to the hyperosmotic interstitial fluid. – In the inner medulla, the duct becomes permeable to urea, co ...
Characterization of Organelles in the Vacuolar
Characterization of Organelles in the Vacuolar

... Extraction from the transformant cells and immunoblot analysis The transformed BY-2 cells were gently filtered to be packed. Each 1 g of the packed cells was homogenized in 2 ml of the following buffers. The buffer of 10 mM Tris-HCl, pH 7.5, including proteinase inhibitors (Complete Mini, Boehringer ...
Plant Cell Walls: Basics of Structure, Chemistry, Accessibility and the
Plant Cell Walls: Basics of Structure, Chemistry, Accessibility and the

... larger and longer fibrils [9]. The cellulose microfibrils are hydrophobic and can be highly crystalline, features that contribute greatly to the recalcitrance of biomass. For example, the 100 crystal face of a microfibril is more hydrophobic than the other faces and selectively binds the CBD (cellul ...
Pdf - Text of NPTEL IIT Video Lectures
Pdf - Text of NPTEL IIT Video Lectures

... 0.5 to 3 micrometer in diameter. In case of Coccobacillus, that is the oval shape, shaped bacteria, or oval shaped bacteria, its size varies from 1.5 to 4 micrometer in diameter. Vibrio is a comma shaped bacteria, and if we see its size, it ranges from 0.2 to 2 micrometer in diameter and 0.5 to 100 ...
Oxygen supply
Oxygen supply

... amount of oxygen that is combined with haemoglobin in the blood (Figure 4). The light is at two wavelengths, red and infrared (650 nm and 805 nm). Each wavelength is absorbed differently by haemoglobin, depending on whether the haemoglobin is saturated with oxygen or not. By calculating the absorpti ...
Fuel Cell book.indd
Fuel Cell book.indd

... been taught or should be expected to know, but to assess what they understand so far about how a PEM fuel cell works. It serves as a baseline assessment, for comparison with the knowledge they display at the end of the challenge, in their final presentation, as well as on the post-test. 1. Distribu ...
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Organ-on-a-chip

An organ-on-a-chip (OC) is a multi-channel 3-D microfluidic cell culture chip that simulates the activities, mechanics and physiological response of entire organs and organ systems. It constitutes the subject matter of significant biomedical engineering research, more precisely in bio-MEMS. The convergence of labs-on-chips (LOCs) and cell biology has permitted the study of human physiology in an organ-specific context, introducing a novel model of in vitro multicellular human organisms. One day, they will perhaps abolish the need for animals in drug development and toxin testing.Although multiple publications claim to have translated organ functions onto this interface, the movement towards this microfluidic application is still in its infancy. Organs-on-chips will vary in design and approach between different researchers. As such, validation and optimization of these systems will likely be a long process. Organs that have been simulated by microfluidic devices include the heart, the lung, kidney, artery, bone, cartilage, skin and more.Nevertheless, building valid artificial organs requires not only a precise cellular manipulation, but a detailed understanding of the human body’s fundamental intricate response to any event. A common concern with organs-on-chips lies in the isolation of organs during testing. ""If you don’t use as close to the total physiological system that you can, you’re likely to run into troubles"" says William Haseltine, founder of Rockville, Maryland. Microfabrication, microelectronics and microfluidics offer the prospect of modeling sophisticated in vitro physiological responses under accurately simulated conditions.
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