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GNOM-LIKE1/ERMO1 and SEC24a/ERMO2 Are
GNOM-LIKE1/ERMO1 and SEC24a/ERMO2 Are

... microscope, we isolated two recessive mutants that had disorganized ER morphology (Figures 1C to 1F) and designated them ermo1 and ermo2. Both ermo1 and ermo2 developed a number of strongly GFP-labeled spherical structures that were ;1 mm in diameter (Figures 1C to 1F; see Supplemental Figures 2 and ...
Respiratory System
Respiratory System

... • The respiratory system is completely lined with mucous membranes – We produce about 1 liter of mucus each day!!! – This forms a barrier and trap system – Respiratory filtering and cilia • Runny noses and sluggish cilia – The folds and hairs in the nasal cavity cause turbulence in the air we breath ...
Formation Costimulation in Immunological Synapse Live
Formation Costimulation in Immunological Synapse Live

... APC and their receptor on T cells, CD28. Numerous studies have shown that B7/CD28 ligation enhances proliferation and IL-2 secretion by CD4⫹ T cells (13, 14). CD28 engagement is linked to cytoskeletal rearrangements upon TCR ligation (5, 15), and cytochalasin D blockade of these rearrangements aboli ...
Terra Nova Live 4/20/00 - Fifth Grade at Gardendale
Terra Nova Live 4/20/00 - Fifth Grade at Gardendale

... Arteries, Veins, Capillaries ...
Human Body Systems DR. I MCSNEER
Human Body Systems DR. I MCSNEER

... hard and dense, but not solid. Small canals run through the compact bone, carrying blood vessels and nerves from the bone’s surface to the living cells within the bone. Just inside the compact bone is a layer of spongy bone, which has many small spaces within it. ...
Barbara McClintock - Nobel Lecture
Barbara McClintock - Nobel Lecture

... In the mid-nineteen-thirties another event inducing chromosome rupture was discovered. It revealed why crossing-over should be suppressed between the centromere and the nucleolus organizer in organisms in which chiasmata terminalize, from the initial location of a crossover to the end of the arm of ...
A partially thrombosed dissecting aneurism of the splenic artery
A partially thrombosed dissecting aneurism of the splenic artery

... treatment is recommended and succesful results have been reported [5,7,8]. For aneurysms with a limited dissection which do not exceed 2 cm in diameter in clinically stable patients, without a perivascular hematoma, annual follow-up is recommended [1,4]. The recent literature reveals that most of th ...
Biological Activities of Compounds Produced by Microorganisms
Biological Activities of Compounds Produced by Microorganisms

... to determine the molecular mechanism of their cytotoxic effect on tumor cells [1]. To determine whether a reduction of viability of tumor cells is associated with apoptosis, the levels of the apoptotic marker - PARP-1 polymerase (Poly ADP-ribose polymerase) were analyzed with the application of the ...
Mader 9e
Mader 9e

... Respiratory control center, located in pons & medulla oblongata of the brain, can change the normal rate according to circumstances. - When a drop in pH is noted (due to increase in CO2) the control center increases rate & depth of breathing. - Normally, O2 concentration in blood has little effect ...
Mechanism of Uptake and Retrograde Axonal Transport of
Mechanism of Uptake and Retrograde Axonal Transport of

... types of membrane during exocytotic transmitter release, vesicle membrane seems to be retrieved selectively (25, 26, 38, 67). Less clear is the origin of synaptic vesicles. Vesicles, especially large dense-core vesicles in adrenergic or serotoninergic neurons, are rapidly transported from the cell b ...
Cell Lineage and Fate Map of the Primary Somatoblast of the
Cell Lineage and Fate Map of the Primary Somatoblast of the

... Because of their stereotypic cleavage patterns and the ability to uniquely identify blastomeres, spiralian embryos are particularly amenable to studies of fate-mapping. In addition to a strict pattern of cleavages, larval fates of identified cells are also largely conserved. For example, each of the ...
The synthesis and migration of nuclear proteins during mitosis and
The synthesis and migration of nuclear proteins during mitosis and

... Hooded rats were given an intraperitoneal injection of 3 H-tyrosine, and killed in pairs 10 min, 30 min, 12 h, 36 h, 7 days, and 30 days later. A piece of skin with white growing hair, and the tongue, were taken from each animal and radioautographs were prepared. Silver grains were counted over whol ...
Bacteria Section 18.2 Summary – pages 484-495
Bacteria Section 18.2 Summary – pages 484-495

... environmental conditions, produce a tiny “escape pod” called an endospore. • An endospore is a tiny structure that contains a bacterium’s DNA and a small amount of its cytoplasm, encased by a tough outer covering that resists drying out, temperature extremes, and harsh chemicals. ...
Targeting lactate metabolism for cancer therapeutics
Targeting lactate metabolism for cancer therapeutics

... poor outcome. Lactate mediates cancer cell intrinsic effects on metabolism and has additional non–tumor cell autonomous effects that drive tumorigenesis. Tumor cells can metabolize lactate as an energy source and shuttle lactate to neighboring cancer cells, adjacent stroma, and vascular endothelial ...
Paul, her is a little story that mite interest you, its how cell therapy
Paul, her is a little story that mite interest you, its how cell therapy

... animals and humans: embryonic stem cells and non-embryonic "somatic" or "adult" stem cells. The functions and characteristics of these cells will be explained. Scientists discovered ways to derive embryonic stem cells from early mouse embryos nearly 30 years ago, in 1981. The detailed study of the b ...
Coding of Border Ownership in Monkey Visual Cortex
Coding of Border Ownership in Monkey Visual Cortex

... T wo experimental setups were used, setup 1 for animals M12 and M15 and setup 2 for animals M13 and M16 (in the results to be presented below, the first digits of the neuron identification numbers indicate the animal). In setup 1, visual stimuli were generated by an Omnicomp GDS 2000 processor contr ...
Pseudolaric Acid B Induced Cell Cycle Arrest, Au
Pseudolaric Acid B Induced Cell Cycle Arrest, Au

... arrest, autophagy and senescence especially the sequence of events in one experiment model. PKC enzymes were shown to play a role in G2/M transition. The suggested mechanism of PKC was suppression of cdc2 activity. But most of the published data strongly implicated PKC in lamin B phosphorylation and ...
Embryonic stem cells in non-human primates An overview of neural
Embryonic stem cells in non-human primates An overview of neural

... expanded continuously in culture. EC cells were also able to differentiate either in vitro in suspension cultures (Martin and Evans, 1975) or in vivo, upon grafting into histocompatible mice, and were even capable to participate in normal embryogenesis after injection into an early embryo (Papaioann ...
BD Pharmingen™ Transcription Factor Buffer Set
BD Pharmingen™ Transcription Factor Buffer Set

... Where can I “stop” in the protocol? Investigators should determine their own “stop” condition for their application. However, here are a few tips: 1. Storing fix/perm samples: We have had success using the 1X fix/perm buffer and then storing overnight at 2–8°C with no manipulations, and then continu ...
the circulatory system
the circulatory system

... which is involuntary, striated muscle, is the composition of the myocardium, or middle layer, of the walls of the heart. The muscle cells of the heart are striated, but the nucleus is more centrally located than it is in voluntary, striated muscle cells. The muscle fibers of the heart tend to intert ...
1. This cartoon shows Complex I in the ETC, in its two alternative
1. This cartoon shows Complex I in the ETC, in its two alternative

... 4. Phosphofructokinase (PFK) catalyzes a reaction in glycolysis—the transfer of a phosphate group from ATP to fructose 6-phosphate, forming fructose 1,6-bisphosphate. PFK also has a regulatory site where ATP binds, but with lower affinity than the active site. (10 pts) a) A mutation changes the conf ...
The C-terminal end of R-Ras contains a focal adhesion targeting
The C-terminal end of R-Ras contains a focal adhesion targeting

... R-Ras uses the endomembrane transport route To investigate the targeting of R-Ras, we constructed constitutive active (38V) and dominant-negative (43N) mutants of R-Ras and tagged them to EGFP. Western blot analysis of Hela cell extracts harbouring these constructs showed the presence of a 55 kDa pr ...
Mechanics and Modeling of Plant Cell Growth
Mechanics and Modeling of Plant Cell Growth

... along the newly created stress patterns [7]. Enlisting engineering methods such as finite element modeling for plant biological applications [7-9] requires an increased and critical understanding of the mechanical and physical concepts and the challenges associated with their adaptation to plant and ...
1 - Purdue Department of Biological Sciences
1 - Purdue Department of Biological Sciences

... protoxylem undergoes severe stretching and is eventually destroyed. Metaxylem is initiated in the still-growing primary plant body, but matures largely after stem elongation is completed. Metaxylem may have spiral, scalariform, reticulate, or pitted elements. In plants lacking secondary growth, the ...
The Expression of MHC Class II Genes in Macrophages Is Cell
The Expression of MHC Class II Genes in Macrophages Is Cell

... could also affect the translational apparatus and regulate in a CCD way the translation of several genes (7, 8). Therefore, there are genes induced in a CCD fashion that are not involved in cell cycle progression. This is the case of genes that control some cellular activities. For example, it has b ...
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Organ-on-a-chip

An organ-on-a-chip (OC) is a multi-channel 3-D microfluidic cell culture chip that simulates the activities, mechanics and physiological response of entire organs and organ systems. It constitutes the subject matter of significant biomedical engineering research, more precisely in bio-MEMS. The convergence of labs-on-chips (LOCs) and cell biology has permitted the study of human physiology in an organ-specific context, introducing a novel model of in vitro multicellular human organisms. One day, they will perhaps abolish the need for animals in drug development and toxin testing.Although multiple publications claim to have translated organ functions onto this interface, the movement towards this microfluidic application is still in its infancy. Organs-on-chips will vary in design and approach between different researchers. As such, validation and optimization of these systems will likely be a long process. Organs that have been simulated by microfluidic devices include the heart, the lung, kidney, artery, bone, cartilage, skin and more.Nevertheless, building valid artificial organs requires not only a precise cellular manipulation, but a detailed understanding of the human body’s fundamental intricate response to any event. A common concern with organs-on-chips lies in the isolation of organs during testing. ""If you don’t use as close to the total physiological system that you can, you’re likely to run into troubles"" says William Haseltine, founder of Rockville, Maryland. Microfabrication, microelectronics and microfluidics offer the prospect of modeling sophisticated in vitro physiological responses under accurately simulated conditions.
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